UniProt UniProt http://www.uniprot.org SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat. Cell Biol. (1465-7392) 2005 imex curation Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Pierre.Gonczy@isrec.unil.ch 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Pierre.Gonczy@isrec.unil.ch Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 davet Only protein-protein interactions imex curation SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat. Cell Biol. (1465-7392) 2005 imex curation Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Pierre.Gonczy@isrec.unil.ch 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Pierre.Gonczy@isrec.unil.ch Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 davet Only protein-protein interactions imex curation SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat. Cell Biol. (1465-7392) 2005 imex curation Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Pierre.Gonczy@isrec.unil.ch 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Interactions in 7c, 7e and 7f-7j not annotated as you cannot precisely determine the protein concerned: for instance the gamma-tubulin antibody used is GTU-88 which recognizes TUBG1 and TUBG2. Pierre.Gonczy@isrec.unil.ch Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 davet Only protein-protein interactions imex curation SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat. Cell Biol. (1465-7392) 2005 imex curation Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Pierre.Gonczy@isrec.unil.ch 01-Feb-2008: Contacted by JYOTI. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Leidel S., Delattre M., Cerutti L., Baumer K., Gonczy P. Pierre.Gonczy@isrec.unil.ch Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 davet Only protein-protein interactions imex curation sas6_caeel Spindle assembly abnormal protein 6 sas-6 Y45F10D.9 protein protein caeel Caenorhabditis elegans MTSKIALFDQTLIASLLQPLSLNQPDFKAYKTKVKLKISEQRNETSGEKELKFEISRSDDFEFLFSETLNNEKYQILARDHDLTVDFDAFPKVIIQHLLCKNIVKNLEEDGEVDARKKAGYHSIADPGKPTEINIILDAEKNFCSFELFSKTPISKGKIFSIKLHAVRGDHLISHLLKICSSQAVKLSTFYKSADELASLRQKCGDLEKQVEKLSGVKEEFEEMSEKFKELEDEVELVKEERENIRLLVEDKEDEVADLKQDTESLQKQLEENQEELEIVGNMLREEQGKVDQLQKRNVAHQKEIGKLRAELGTAQRNLEKADQLLKRNSQQQNQQSLDMRKLGELEADLKEKDSMVESLTETIGILRKELENEKLKAAENMDSFEKLSMENENLKEKIAHYRAQRFSPAPSGLPGLQTGLTNRLTPSFKPVLGPHTPYGANLNSRTPFRDNTTLNFQNSTIATPHAFRFNSQLIADETTGSSVTNTPPAQR 118ED9B5EF6357A6 sas5_caeel Spindle assembly abnormal protein 5 sas-5 F35B12.5 protein protein caeel Caenorhabditis elegans MNNYDDLPCSIYFKKPTVQEFVDQPRVFEDSEVPAFQEVLQLPQERTKPPVPSTQPIVAAVEVAKKKSCLSAPKPRKEPPSHPALRQKTVAFGKTVNVSQTVEGTSRNSKKVLASTMSAQNTTTTEEQAAENWRDAMKTELQTIRTEIQEETARRQEELNAQNLVKMQELMSNFFQKITIPKQQAIEPVEKDKENFHESPRQSRQQKPASKIASAREVIKRDGVIPPEALTIIEQRLRSDPMFRQQIDNVLADAECDANRAAYSPPPPMSEVRYGSGVNPALMRETLTVERSIRYDNGLASIDSRQWTNERRDNRAPDSYRTYEPDQPCHSLYQKGQSISYYPSEAAGKTTARNNRTGYYVEDSSDHEEDVVVNKRGQNYHEQAVPETPAERERRIREKYARRK D86EBE73413E5DAE sas4_caeel Spindle assembly abnormal protein 4 sas-4 F10E9.8 protein protein caeel Caenorhabditis elegans MASDENIGADGEQKPSRPFLRKGQGTARFRMPRNNKTSAGAPPTSELSSASSPSINVPRFSLSNALPNSARTVDSGISNEDETRPPTTASLPMDQPSLSSSPENRLNPAPSVAEEHGHSGQHAEEEEDNDTDEVSAMPSFVPDEPSTLVNSDHELSDDALKYKNAAAEFKAFERRMDSMRSASTITTSLATPSSCAPSNSSEPPTRSTPIMNDLGVGPNNHNWPSSMQELSGISLETPQARPLGSNRINQLVRSEAQTGISLLQHHERPTVTAPLRRNDMMNSSRQNPQNGNVQDENRPEHVYDQPIHVPGSSLDRQKLEIEIRRHRNLNIQLRDTIAHLDYAEESVHTTKRQLEEKISEVNNFKKELIEEFKKCKKGVEEEFEKKFEKIKEDYDELYEKLKRDQRDLERDQKILKKGTGERNKEFTETIATLRDKLRASETKNAQYRQDIRVRDEKLKKKDEEIEKLQKDGNRLKSTLQTLEKRVKQLRTEKERDDKEKEMFAKVAMNRKTSNPVPPVLNQSVPISITSNGPSRHPSSSSLTTFRKPSTSNRERGVSWADEPNEQSLEAVPQEFLMMPVKEMPGKFGKCTIYRDSLGETSKVTDTIANGLLFEYSNGDLRWVNRQNAVNIYISAVDKTVRIDLPTYNISIIHTFQRQVEVLRPGNNITLISIKRREVRTDLIYQNGMYKTEIFNRDGRYVTKDFSNQEVSRKYNPGTHTYRDNQCRYVLVTDYNDFELVEPEFRLRWYQGDPTGLNNQYILKIIGRPECSEKTLRLEVNLSTCEGTLETAEMIGDKRRKTTLFQWKK 722C4509D3ACA97B cp250_human Centrosome-associated protein CEP250 Centrosomal protein 2 Centrosomal Nek2-associated protein 1 CEP250 CEP2 CNAP1 250 kDa centrosomal protein protein protein human Homo sapiens Human METRSPGLNNMKPQSLQLVLEEQVLALQQQMAENQAASWRKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMIKALRETVEILETNHTELMEHEASLSRNAQEEKLSLQQVIKDITQVMVEEGDNIAQGSGHENSLELDSSIFSQFDYQDADKALTLVRSVLTRRRQAVQDLRQQLAGCQEAVNLLQQQHDQWEEEGKALRQRLQKLTGERDTLAGQTVDLQGEVDSLSKERELLQKAREELRQQLEVLEQEAWRLRRVNVELQLQGDSAQGQKEEQQEELHLAVRERERLQEMLMGLEAKQSESLSELITLREALESSHLEGELLRQEQTEVTAALARAEQSIAELSSSENTLKTEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEEENQSVCSRMEAAEQARNALQVDLAEAEKRREALWEKNTHLEAQLQKAEEAGAELQADLRDIQEEKEEIQKKLSESRHQQEAATTQLEQLHQEAKRQEEVLARAVQEKEALVREKAALEVRLQAVERDRQDLAEQLQGLSSAKELLESSLFEAQQQNSVIEVTKGQLEVQIQTVTQAKEVIQGEVRCLKLELDTERSQAEQERDAAARQLAQAEQEGKTALEQQKAAHEKEVNQLREKWEKERSWHQQELAKALESLEREKMELEMRLKEQQTEMEAIQAQREEERTQAESALCQMQLETEKERVSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGILQTQLQEAQRELKEAARQHRDDLAALQEESSSLLQDKMDLQKQVEDLKSQLVAQDDSQRLVEQEVQEKLRETQEYNRIQKELEREKASLTLSLMEKEQRLLVLQEADSIRQQELSALRQDMQEAQGEQKELSAQMELLRQEVKEKEADFLAQEAQLLEELEASHITEQQLRASLWAQEAKAAQLQLRLRSTESQLEALAAEQQPGNQAQAQAQLASLYSALQQALGSVCESRPELSGGGDSAPSVWGLEPDQNGARSLFKRGPLLTALSAEAVASALHKLHQDLWKTQQTRDVLRDQVQKLEERLTDTEAEKSQVHTELQDLQRQLSQNQEEKSKWEGKQNSLESELMELHETMASLQSRLRRAELQRMEAQGERELLQAAKENLTAQVEHLQAAVVEARAQASAAGILEEDLRTARSALKLKNEEVESERERAQALQEQGELKVAQGKALQENLALLTQTLAEREEEVETLRGQIQELEKQREMQKAALELLSLDLKKRNQEVDLQQEQIQELEKCRSVLEHLPMAVQEREQKLTVQREQIRELEKDRETQRNVLEHQLLELEKKDQMIESQRGQVQDLKKQLVTLECLALELEENHHKMECQQKLIKELEGQRETQRVALTHLTLDLEERSQELQAQSSQIHDLESHSTVLARELQERDQEVKSQREQIEELQRQKEHLTQDLERRDQELMLQKERIQVLEDQRTRQTKILEEDLEQIKLSLRERGRELTTQRQLMQERAEEGKGPSKAQRGSLEHMKLILRDKEKEVECQQEHIHELQELKDQLEQQLQGLHRKVGETSLLLSQREQEIVVLQQQLQEAREQGELKEQSLQSQLDEAQRALAQRDQELEALQQEQQQAQGQEERVKEKADALQGALEQAHMTLKERHGELQDHKEQARRLEEELAVEGRRVQALEEVLGDLRAESREQEKALLALQQQCAEQAQEHEVETRALQDSWLQAQAVLKERDQELEALRAESQSSRHQEEAARARAEALQEALGKAHAALQGKEQHLLEQAELSRSLEASTATLQASLDACQAHSRQLEEALRIQEGEIQDQDLRYQEDVQQLQQALAQRDEELRHQQEREQLLEKSLAQRVQENMIQEKQNLGQEREEEEIRGLHQSVRELQLTLAQKEQEILELRETQQRNNLEALPHSHKTSPMEEQSLKLDSLEPRLQRELERLQAALRQTEAREIEWREKAQDLALSLAQTKASVSSLQEVAMFLQASVLERDSEQQRLQDELELTRRALEKERLHSPGATSTAELGSRGEQGVQLGEVSGVEAEPSPDGMEKQSWRQRLEHLQQAVARLEIDRSRLQRHNVQLRSTLEQVERERRKLKREAMRAAQAGSLEISKATASSPTQQDGRGQKNSDAKCVAELQKEVVLLQAQLTLERKQKQDYITRSAQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRRLDESLTQSLTSPGPVLLHPSPSTTQAASR DIP protein Q9BV73 original sequence version: 129 BC2B8A36E07B8272 cetn3_human Centrin-3 CETN3 CEN3 protein protein human Homo sapiens Human MSLALRSELVVDKTKRKKRRELSEEQKQEIKDAFELFDTDKDEAIDYHELKVAMRALGFDVKKADVLKILKDYDREATGKITFEDFNEVVTDWILERDPHEEILKAFKLFDDDDSGKISLRNLRRVARELGENMSDEELRAMIEEFDKDGDGEINQEEFIAIMTGDI EF67724B2D6C2680 sas6_human Spindle assembly abnormal protein 6 homolog SASS6 SAS6 protein protein human Homo sapiens Human MSQVLFHQLVPLQVKCKDCEERRVSIRMSIELQSVSNPVHRKDLVIRLTDDTDPFFLYNLVISEEDFQSLKFQQGLLVDFLAFPQKFIDLLQQCTQEHAKEIPRFLLQLVSPAAILDNSPAFLNVVETNPFKHLTHLSLKLLPGNDVEIKKFLAGCLKCSKEEKLSLMQSLDDATKQLDFTRKTLAEKKQELDKLRNEWASHTAALTNKHSQELTNEKEKALQAQVQYQQQHEQQKKDLEILHQQNIHQLQNRLSELEAANKDLTERKYKGDSTIRELKAKLSGVEEELQRTKQEVLSLRRENSTLDVECHEKEKHVNQLQTKVAVLEQEIKDKDQLVLRTKEAFDTIQEQKVVLEENGEKNQVQLGKLEATIKSLSAELLKANEIIKKLQGDLKTLMGKLKLKNTVTIQQEKLLAEKEEKLQKEQKELQDVGQSLRIKEQEVCKLQEQLEATVKKLEESKQLLKNNEKLITWLNKELNENQLVRKQDVLGPSTTPPAHSSSNTIRSGISPNLNVVDGRLTYPTCGIGYPVSSAFAFQNTFPHSISAKNTSHPGSGTKVQFNLQFTKPNASLGDVQSGATISMPCSTDKENGENVGLESKYLKKREDSIPLRGLSQNLFSNSDHQRDGTLGALHTSSKPTALPSASSAYFPGQLPNS 6D19696BC748849F tacc1_caeel Transforming acid coiled-coil-containing protein 1 2P40 tac-1 Y54E2A.3 protein protein caeel Caenorhabditis elegans MSLNTTFTKEDGTEVVIPFNGSQNGHPENEEPEVEEAAEPSSSVETLCGATRGDIIVMKHTTKALTELIERLLHSDEFEVRRCSNGQIISQGRCNGTTPGNGIGGGGASSEELEKALKDRDAARAEADKLHANYATLFASFNTVREAANDIRGEYEDARDKLKLAAAEVDEWQAKFLAVKDNANSELERASVEYDDLLRSHDENTKGLRLRVKRQEIELSSKNDEIKVLTNRVSELSQICDQLLNDVDVSDGMSVISTDA 31D20FF3E5AF562B sas6-sas4-1 2 7 unspecified role unspecified role neutral component neutral component n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 3d sas6-sas5-1 2 6 unspecified role unspecified role neutral component neutral component n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 3e sas6-sas4-2 2 7 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig 3f, 3g sas6-tac1-1 2 11 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig 5a-5d, 5h sas6-sas5-2 4 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey Q20010:p.Arg397Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R C Wed May 02 14:31:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg397cys' Tue Jun 30 17:36:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg397Cys' physical association physical association Fig 6a, 6b sas5-sas6-1 4 6 unspecified role unspecified role bait bait Q20010:p.Arg397Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R C Wed May 02 16:45:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg397cys' Tue Jun 30 18:53:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg397Cys' 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 6a, 6b sas6-sas5-3 1 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig 6e sass6-cep250-1 3 10 unspecified role unspecified role neutral component neutral component n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 7a, 7b sass6-cetn3-1 3 10 unspecified role unspecified role neutral component neutral component n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 7d