IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular characterization of the cytoplasmic interacting protein of the receptor kinase IRK expressed in the inflorescence and root apices of Arabidopsis. Molecular characterization of the cytoplasmic interacting protein of the receptor kinase IRK expressed in the inflorescence and root apices of Arabidopsis. Biosci. Biotechnol. Biochem. (0916-8451) 2004 Hattan J., Kanamoto H., Takemura M., Yokota A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp 22-Jan-2008: Contacted by JYOTI. Arabidopsis inflorescence cDNA library yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Hattan J., Kanamoto H., Takemura M., Yokota A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp Accepted 2007-DEC-20 by cderow Arabidopsis inflorescence cDNA library Biosci. Biotechnol. Biochem. (0916-8451) 2004 Molecular characterization of the cytoplasmic interacting protein of the receptor kinase IRK expressed in the inflorescence and root apices of Arabidopsis. Molecular characterization of the cytoplasmic interacting protein of the receptor kinase IRK expressed in the inflorescence and root apices of Arabidopsis. Biosci. Biotechnol. Biochem. (0916-8451) 2004 Hattan J., Kanamoto H., Takemura M., Yokota A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp 22-Jan-2008: Contacted by JYOTI. Arabidopsis inflorescence cDNA library in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Hattan J., Kanamoto H., Takemura M., Yokota A., Kohchi T. tkohchi@lif.kyoto-u.ac.jp Accepted 2007-DEC-20 by cderow 22-Jan-2008: Contacted by JYOTI. Arabidopsis inflorescence cDNA library Biosci. Biotechnol. Biochem. (0916-8451) 2004 irki_arath IRK-interacting protein IRKI T24H18.70 At5g12900 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASSETRMNKAREKEEAIANGVKLRALQASLMQMKSSPSSNYSLRNPSSSSAASPASRPLPNLSAHDYPVFTPSYEDEPVSAFHHKNLTLSETWDEDGVGLVDGDTYLSDSYKTSTSRKTVMMPHQDSHHHVYTMSDALRSPPLHFYTTGRSNCGSVDFRSVSSCNDYNKQKGFDTKSLKSSNLVVPLTDSHSAVVSSQPRNRGGRVMSWLFPKLKKKQKSNSIFNSPSITEKSEEVSEVLKDSGSGVEKLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNLKKALREATEVVSQENSGGRSSGKKNSEMPVSEEVMVEGFLQIVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLLQPHNLSFTSKYSKIIQYHLEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEENREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFYVLDRVLRCKVLCRYKSLG CFDB25EC6ED529F0 irk_arath Probable LRR receptor-like serine/threonine-protein kinase IRK IRK Inflorescence and root apices receptor-like kinase T5P19.20 At3g56370 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYKALIFTVLLVSAVAPVRSLDPPLNDDVLGLIVFKADLRDPEQKLASWNEDDYTPCSWNGVKCHPRTNRVTELNLDGFSLSGRIGRGLLQLQFLHKLSLSNNNLTGIINPNMLLSLVNLKVVDLSSNGLSGSLPDEFFRQCGSLRVLSLAKNKLTGKIPVSISSCSSLAALNLSSNGFSGSMPLGIWSLNTLRSLDLSRNELEGEFPEKIDRLNNLRALDLSRNRLSGPIPSEIGSCMLLKTIDLSENSLSGSLPNTFQQLSLCYSLNLGKNALEGEVPKWIGEMRSLETLDLSMNKFSGQVPDSIGNLLALKVLNFSGNGLIGSLPVSTANCINLLALDLSGNSLTGKLPMWLFQDGSRDVSALKNDNSTGGIKKIQVLDLSHNAFSGEIGAGLGDLRDLEGLHLSRNSLTGPIPSTIGELKHLSVLDVSHNQLNGMIPRETGGAVSLEELRLENNLLEGNIPSSIKNCSSLRSLILSHNKLLGSIPPELAKLTRLEEVDLSFNELAGTLPKQLANLGYLHTFNISHNHLFGELPAGGIFNGLSPSSVSGNPGICGAVVNKSCPAISPKPIVLNPNATFDPYNGEIVPPGAGHKRILLSISSLIAISAAAAIVVGVIAITVLNLRVRASTVSRSAVPLTFSGGDDFSRSPTTDSNSGKLVMFSGEPDFSTGTHALLNKDCELGRGGFGAVYRTVIRDGYPVAIKKLTVSSLVKSQDEFEREVKKLGKLRHSNLVKLEGYYWTTSLQLLIYEFLSGGSLYKQLHEAPGGNSSLSWNDRFNIILGTAKCLAYLHQSNIIHYNIKSSNVLLDSSGEPKVGDYGLARLLPMLDRYVLSSKIQSALGYMAPEFACRTVKITEKCDVYGFGVLVLEVVTGKKPVEYMEDDVVVLCDMVREALEDGRADECIDPRLQGKFPVEEAVAVIKLGLICTSQVPSSRPHMGEAVNILRMIRCPSGSSDELGSS 3A3D17F5A7C26FFC irk-irk1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association I cannot cut and paste out source text for this pdf - I leave this annotation blank. Table 1 irk-irk1-1 2 4 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association I cannot cut and paste out source text for this pdf - I leave this abnnotation blank. 2