UniProt UniProt http://www.uniprot.org The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. J. Cell. Sci. (0021-9533) 2004 Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp Accepted 2008-FEB-23 by CDEROW. 03-Mar-2009: Contacted by JYOTI. J. Cell. Sci. (0021-9533) 2004 The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. J. Cell. Sci. (0021-9533) 2004 Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp Accepted 2008-FEB-23 by CDEROW. 03-Mar-2009: Contacted by JYOTI. A commercially supplied S. pombe cDNA library (Clontech) J. Cell. Sci. (0021-9533) 2004 The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. J. Cell. Sci. (0021-9533) 2004 Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp Accepted 2008-FEB-23 by CDEROW. 03-Mar-2009: Contacted by JYOTI. Clontech S.pombe cDNA library. J. Cell. Sci. (0021-9533) 2004 The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. J. Cell. Sci. (0021-9533) 2004 Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Niccoli T., Yamashita A., Nurse P., Yamamoto M. The tubulin/ssm3 costaining was not annotated as there are 2 alpha tubulin isotypes in S. pombe, both of which may react with the anti tubulin antibody used. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp Accepted 2008-FEB-23 by CDEROW. 03-Mar-2009: Contacted by JYOTI. J. Cell. Sci. (0021-9533) 2004 The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. J. Cell. Sci. (0021-9533) 2004 Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp Accepted 2008-FEB-23 by CDEROW. 03-Mar-2009: Contacted by JYOTI. J. Cell. Sci. (0021-9533) 2004 The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast. J. Cell. Sci. (0021-9533) 2004 Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Niccoli T., Yamashita A., Nurse P., Yamamoto M. paul.nurse@mail.rockefeller.edu,myamamot@ims.u-tokyo.ac.jp Accepted 2008-FEB-23 by CDEROW. 03-Mar-2009: Contacted by JYOTI. Library from pat1 deletion strain undergoing synchronous meiosis. J. Cell. Sci. (0021-9533) 2004 tip1_schpo Tip elongation protein 1 tip1 noc1 SPAC3C7.12 Protein noc1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFPLGSVVEVITGERGFVRYAGEVENRKGVYVGLELLPEFAEFGKNRGVVDGREYFKTKNNEKTGIFVPFDKCKLASSISSSPSPKIDGTAASIGMGFPPMSPNLQSSIPRLTNVSSSSNLSMNTISSTALTPTEKILQKRIEDLLYERQNHQQQLEEVLATVDQLQSLVTNFNDQQDEVDELRERITLKEERIQQMRNEASQRRFEFKTTIECLEESSNRAIETYENRIAELEAQLEMYMSGKSEDDLLFSLQQERDYALNQVEILQERVDTLMKQKANSSTANEKLSHMESSSPTLTNASFESPKRGKGSNDLPENHPQRRQTLEFYEIEIEVLREKVEKLQALSDEKDFYISKLEKSLDRNDTTPVPSDEKLSNYAAEKENLVSRISELEHTIEQLTINNERDNERMSPAEFELETTQEVEENDSDSHDDEETWCEVCETNNHSLQECPTVFGSTDEA 66CCDB96146F86D6 66CCDB96146F86D6 dyhc_schpo Dynein heavy chain, cytoplasmic dhc1 SPAC1093.06c SPAC30C2.01c Dynein heavy chain, cytosolic protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDKNDNSQCQLSTVSDEILIFLKKLLSLSVSDNLDDICETIALQSTFVDTFRSFINDEYYTVIYLYGSPCLTSSPTSPDSCTFGNMTFQWTSLLQDVFSGTSAFAIFKRFPLTDTPLTLQNMLYINQLPILKGGHKSNEIPERPINAIFLYTKYVMSCYFTAYLAMESVDDRSTIDLNSPSKGKELELTCQKFADFERSFTFFCREYQNSDTILQHHPLILSTIKHAEENNLDLSVRLLPSKVLSDSEFYKSLSNLVNVWLKTTRSLIKLFHDQISKTALEEFNFWQFYYRSLSRLNDQLHSRPVLFVLDILAFGKRFHTIASFNSETNIQCFVDKVGKIDALFKEISLDIFLSSSTLESLQLSAALLYSTFSKKWRNTGYPETRVLDFINFITEDLLKLISRLLPALGALSLSNVDFSHRTAVSSDILSLCYIRLKDFLRISGSLKEEQSYYGLKNSIKQIKAFENKLKYIQSFHEKHQQLIGALSEVYGLTHLTELEILEHLNKKEHVFNILTVFKDLQSLNVLDISLKGVNAWNSLETSYYNCMTVLEDEVIAQLKSLLQYSKTSSQMFTTLMRFQPLFFRTRVRTSISDCLHLLVNRIKQELDLLKTRFTEDVSDTELIAMNELRNLPMASSAIIWATQLKNRLHEYTKNINIIFGEDWNNFPDGFELKVECITLQKRLDTNLIFTNWINDVSSRNLNFDFDSKIFYLTQSESAESPLRLSVSIDFDPCSFCKEIRTLAHLGYNIPSQLMELASCLQRIQLIAMCLIDSVQSFNDVSFEISKTEEERFLLQEYELAVRQHIVTGLFISWNDFIVGNLSTPPKCAIGKRNFLKNIHPNVENYAYQFSSLTSLLMNKRNAISHTYMQIQEQLFQLDICEYSGDIFLTIQRKLQDLIDLLYVNGYSNLPPFVRALNLRFQDLLISRCRKFLSFFKTTILTSGNENNDLKSKFSSDMYEKLRGFLKPTNLTIQRNIIEFDPPVYRKKEDSIYLLDMCLQSVVNIPLLSIKTTAQRNCTLIGFFPVINRLESEILGIFESLLFHFDSILGYQNYWKKVEPFLNLNSLNLLLKSELFQLDQCYSLSLYLIHLKSEVDEIGKVTDFKIFSVNNTEFKSQVYLYLREWINALFDRFTFLLGKESEHLLNELDDTHSSLSTVDFTVNNTESLINSLKIFKKAGCYKLNVEHKIITYQNYEMTFNDCDAFSEFNFSLLKDITSKWKDLLESFECRRLKLENNKDEILRNFSEFAKRVNTETLSLISEWCASSLSLIKANYDEFSSTVDDFLFRFSKATEQCLMVKYIKKDLEIEIEESCDFSIQTEEIHLYKKFKDVISSNLEFIVEIKNTRWKLFDTATLSVQTTHQINALESVHTSFQHFKLFTNTKQSLNQLKDCTLLLQKLKSCPLKPVHWISLFEITKSTEQLDFEKLLVSDILGIDLQAHESFITTLLNSAVVEANLENQFNEVHSFWKNSYFSFKSFKGRNYIVVGCQELIDAVEKNMDSLNLIKTSRHFKDGDMNITDLQSKMKIIVKFLNIWKEIQQIWTHLSAIFYESTYIQQLLPELAASFFNSSKTYMHLVTLLKERSYLYKVSNIPSLLESAAKLSTTLEDSKKSLLKYFELQRHKISRLYFLGDDDLMELISNPCDPFVINKQIIKLYPGIRSLIVDTENTNINGCTTNEGNELLFDNPICLLDNTQPLHWISSLEPFLKATLFQLFSTSFQQIRDFYYNKSRNVFCKEWFLRYPSQITLLSLRCTLCHEIETGIADCCLDAVFNFINDGISSLVLLADENELSIKKKVTLMFNELLHFKETVGLLCKNSFNNYFWSREVKAFYREDHDDEAVVIKMFSLEFIYAFEYSELDDPIVYTDLTRNCFSVLLHSIASNLGGSPIGPAGTGKTETVKAVSAYLGKNVFVFNCDNAFNYKTIQRILSGLAQIGTYICFDEFNRLDSGTLSAISYDIQRIQSLVSHSDGLCQSPILLDAPTIFVTMNPGYLGRFKLPSNLKKLFRPIWMGSPDNKKICEILFLSFGFKESSLLSQVLDSFFLCCSGSLSNCLHYDFGLRAMKVVIKAAKRIKGFLKKKNTICQELEILWYAIREVLYPSLIYQDIPLFFKAEESYFNFPAVKANAFIDPDNFEVNIEQTLSKNFFGNNQYLKLKIMQLYQMSEAYNGIILLGKTGSGKSQIFRILQSALLNIGIDCIVYVISPKALTKESLFGSMNMDTREWTDGVFTKLLRKTRDSCYYKRYMFVFDDELSPEWVEAMNSLLDDNKTLTLSNGERIALQPYVKIFFEADSVASLTRATISRCGLICISNIDDNILSSTDKMLSFTSGATNYPLGSSNDEFSTVFSKVLTDEVMMNLISSCYKFSVDLQHIMNFTKQRFFTTFYSLLDQTKLFTRSSNITESLSFKELCNYLKKKICYILAWCCTGDTDAKSRERFTHWLMQNASVDLPEIKDFEHVSILDFDVSLETQSWYPIAGKTLKSSALKYAGNTVIPTLDTVRYAEFLNFSLTKNRCVIFCGPPGSGKSMLMLGTLRSRQDVEVIALNFSISTSSKSVVSFLEQSTVYYRSTGMTIMCPKNHEKVLVLFCDEINLPRSRNCLAEDVICFLRHMLEHQGFWHPLHKEWVTIKNIFVCGACNPSTDIGRNDFPERFLRRTVLIFVDYPESYSLVTIYNALLEKSALINQYKTIILNIVKASVKFYQVLRENFKSSTQGYVYTPRDLTRWLISFKNYAESYAETNNLSLIKVWYHEACRVLLDRLVSQKECSWGMTELQKVIVTDFGEFEVSVIFEKQIIFTDILKNGLEFLDFASLRPKLESLYKKFYSSHPNNTLVFVDETITHILRFHRILNNSGMHALLQGSVGLGQKAVVEFVCWLNSFSLFELQKNQTYSIEDFEDNLKSILILAGTTNCKACLAINESIAGVPGFLDLLNNLLTNSEVSNFFDQNDWAEIKKNLNKLNEFQPLKFDSEESVTEIFMNNVFQNLCVVFYVYTSADVDFQTNSLSPALLNRCTIDYYHSWDYHSMLQIANEVLQETISLNALDHDNPNLKNIKGSSIYDAVAQAVVNTHTSIVWEFKHLGKTSYFSCLHFIRFLNTFCLIFGRDANKLSKEKSRIENGFKKIKETSQGIDKFKEALSDQQNVLFSKTKTANDRLQCIIQTKQAVEAKKVYSLQAEASLQKKSFLLNEKKNSVMKEVSYAKPAVIEARKSVSDIKKAHLIELRSLSRPPMAIRITMEVVCKLLGFSATDWKNVQQLLKRDDFIPKILNYNLEKELSINLRRKIEQDYFSNPIFTFDSVNRASKACGPLLLWIKSICNYSKVLEKLEPLNSEVDRLKLEQKNAEECIQETIAACKDLDEKLLQLQEEYASMISEIHSMELQMDEVKCKMQRSIEVITDLSIERNEWSGFLNLYPKRMWNLVGESLMEASFVVYAGNLDPSMRIFLRNKCEPIISSFGFPISKSAVRTNIERCVQTSIESKYYKNLTDYSLENIYIIQENKSPLLIIDPSSQILDILPSLYKGKASDLISFSNKSFQNQIKLALLSGSAIIIKDAELWDVSIEPLLKPEFFTGSGEVQTTFAKDTITITLPLNIIFFSEVQSNELENKASKFMNVVNFTLSISLLETQMLKSVISVQEPGVFKQKDNCFTLKLSIERQIRSLQEQLLKTLCSSNENIVGTDEIVVLLKNLKEKHETIRLAYSESQSINRKVDELIRRYKLSIKSFLSVVVVFQHFISLKKSYSFSFNFIWSTFHQMLNVVLENRNQDFKSLIMDALRDLIRRCFLYIFPEDRVLFLFLLMFFFFPKETESLRKLLIVNGKTLELEQSYLNFFETCSDSNERGGLESLFFKTHASNIQNFCTEVLANTHCEEDCLKLLYDLWSSAFKVEFSNIKYDFLKIINDESESRMPTIVYLMENCEIDSLLQNAKIPQNIKKLTVSLGSAENESLADSYLKLASTEPLWLFINNIHLSTPWAEKLPSKMSNHLHKNSRIVCLSEIHNQLPHQLLCISRSIVFNKQTSFKNNLLNLLELLPTMTHTLPHNRFRLFFFLSWLHATLAEIYCFTCSSWKEPCYFDDSDFYFGTKILCNILYRNVHLEEFSWGTFKDLLLNVVYGPKVSASSDFIALDKILKRLIAQFKTQISSNILLTDNFKFILPYEITFSSAKEVIGQLPDEIPPGWLDIPENSKRKRTDIYFSMCI 8F10AE370184FC0C tba2_schpo Tubulin alpha-2 chain tub1 alp2 atb2 SPBC800.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSMEVDYMEEEY D5FBA90418FC8493 sad1_schpo Spindle pole body-associated protein sad1 sad1 SPBC12D12.01 SPBC16H5.01c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFTNTPVGGKRERQNGAHPAWSTLGANSAQIHQNTADLASKMHKLRYTKIRSPPTRVSIESITPKQRFPAPNFEQAYHSNIRYEQEESDNEEFENVVKNGHEASTNVFYESDGDDEEFVNEEYENSIDEESDDEGYSLNEDTTATNASFRYPMNQRSTRKSQFYSSKFKPLLWFGITLFSTLLIITLLHKGQEFYSRSFSSDNSQPSNSPVPNIPPASNDTKTSLKPDIIKDFTDSPSKVGGNEEFDYSTGDLITKKEFDKILQQKVEQLKQSLKEEMSNYKSSVPFEVELNDDWKFFIESTVRKYLTDPVSMPNFALLSTGAEVLPALTSKRYVRRPSAFIPRFTSYFFDSLVVRGHEPSIALTPNNAVAMCWSFQGSEGQLGISLSRPVYVTNVTIEHVQHKIAHDLSSAPKDFELWVQGMSSKMFVLLGKARYSLTEDSIQTFSFESSNYIVAEPIQNVILKIKSNWGNPNYTCLYQVRVHGTVPNADEQPIPSLGEKAESTAENTGQDSS DCA4CAC1E1F98F0D dylt_schpo Dynein light chain Tctex-type TCTEX-1 protein homolog dlc1 SPAC1805.08 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSCPIDSKKLEEICLEAAQPVLKASEYDGDKTAEMNQSVIYAVLNALNKETQSYKWIVSSTLVQKLPEDHPSRGVHAAHAACWNCEKDGMTTIKESGEAIDVVLSIMWISI 8F7E424079D2FF6A ssm4_schpo Microtubule-associated protein ssm4 ssm4 SPAC27D7.13c SPAC637.01c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSYLSVGDEVLIRGELGIVRFAGSTDFESGIWLGVELLNGKGKNDGSVKGKRYFSCEKGKGIFVRACSSNVMKRPSVVKSRKKGSENISNFMEKTKAIKQKSRREPSKFERSLARPLCITPIDSSTPTKTATFYTSSTTENLDELNFSTEELSSFDTTLLNSDTSKLSGLDDSSFMEEEFVWQVDNVLQECEKKFTPHSKGSYLKENLKSELRKGRLDELMCENTALKEKIDKLNKELEKVEPQLTFLRSKNSIEKPRNFRREKFLKKFLAMQKEIKYLRKRKLQIRKIPNYKYSDRSLNSKTPKSQDNWTTQVTPSSLLGVSEVSKVLQLKQVQVDITELVKIPKNPFSEKLTISNVNRYLNIVPGSLDLQFSLTNENFVHWNSTVYQELLNLKSNNSSVDGVKTRRQLLEENALLSHKVLKLTEEIQDLETLNQLNTEIEARQSEKLNEVQEETQRLSQLLISSQPALTEVKHLKLCLSDSQEELLQLNAKLEKANIVIDELNSAKLKLSKQVEEESSMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTARMKLADKYPQGDNTSENIDWLKHSLRDSNTENSIPSPLTFACKEIRKLVADIKPVSVEKQLALNWKKDIERPSFHHNQQLFNYCQLTDILSKKC 011B7D740211B57C ssm4-dhc1-1 5 8 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role bait bait c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 3B dhc1-ssm4-1 5 12 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 3B ssm4-dlc1-1 3 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association tub1-ssm4-1 1 12 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component n-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Fig 3D sad1-ssm4-1 4 12 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 5A tip1-dhc1-1 1 8 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 unspecified role unspecified role neutral component neutral component c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig 6F ssm4-dlc1-2 6 12 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association ssm4-dlc1-3 2 12 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Fig 3C