IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J. Virol. (0022-538X) 2004 Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 3 hybrid three hybrid 3-hybrid tri hybrid tri-hybrid assay 3 hybrid predetermined predetermined participant predetermined Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr Accepted 2007-JUL-20 by JYOTI. 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. J. Virol. (0022-538X) 2004 RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J. Virol. (0022-538X) 2004 Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr Accepted 2007-JUL-20 by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. J. Virol. (0022-538X) 2004 RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J. Virol. (0022-538X) 2004 Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr Accepted 2007-JUL-20 by JYOTI. 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. J. Virol. (0022-538X) 2004 RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J. Virol. (0022-538X) 2004 Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. no Interaction shown by the authors to be indirect - requires viral protein as a bridging protein - so put in separate expt to prevent UniProt/GO export. Cells were infected with rotavirus RF at an MOI of 10 PFU/cell. poncet@vms.cnrs-gif.fr Accepted 2007-JUL-20 by JYOTI. 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. J. Virol. (0022-538X) 2004 RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J. Virol. (0022-538X) 2004 Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. Cells were infected with rotavirus RF at an MOI of 10 PFU/cell. poncet@vms.cnrs-gif.fr Accepted 2007-JUL-20 by JYOTI. 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. J. Virol. (0022-538X) 2004 RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J. Virol. (0022-538X) 2004 Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr Accepted 2007-JUL-20 by JYOTI. 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. J. Virol. (0022-538X) 2004 RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J. Virol. (0022-538X) 2004 Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. poncet@vms.cnrs-gif.fr 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. African green monkey kidney CV-1 cDNA. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vitour D., Lindenbaum P., Vende P., Becker MM., Poncet D. Cells were infected with rotavirus RF at an MOI of 10 PFU/cell. poncet@vms.cnrs-gif.fr Accepted 2007-JUL-20 by JYOTI. 29-OCT-2007: Contacted by JYOTI. 2007-06-11: Pierre Lindenbaum, Integragen. J. Virol. (0022-538X) 2004 z3h7b_cerae_protein Zinc finger CCCH domain-containing protein 7B protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to Q9UGR2 if4g1_human Eukaryotic translation initiation factor 4 gamma 1 p220 EIF4G1 EIF4G EIF4GI EIF4F protein protein human Homo sapiens Human MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN DIP protein Q04637 original sequence version: 68 DIP protein Q04637 original sequence version: 166 DIP protein Q04637 original sequence version: 160 DIP protein Q04637-1 original sequence version: 94 DIP protein Q04637 original sequence version: 115 DIP protein Q04637 original sequence version: 119 DIP protein Q04637 original sequence version: 146 DIP protein Q04637-1 original sequence version: 148 DIP protein Q04637 original sequence version: 150 324088B60863DA34 nsp3_rots4 Non-structural protein 3 Non-structural RNA-binding protein 34 NCVP4 protein protein rots4 Rotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) Simian Agent 11 (strain 4F) MLKMESTQQMAVSIINSSFEAAVVAATSALENMGIEYDYQDIYSRVKNKFDFVMDDSGVKNNLIGKAITIDQALNNKFGSAIRNRNWLADTSRPAKLDEDVNKLRMMLSSKGIDQKMRVLNACFSVKRIPGKSSSIIKCTKLMRDKLERGEVEVDDSFVDEKMEVDTIDWKSRYEQLEQRFESLKSRVNEKYNNWVLKARKMNENMHSLQNVISQQQAHIAELQVYNNKLERDLQNKIGSLTSSIEWYLRSMELDPEIKADIEQQINSIDAINPLHAFDDLESVIRNLISDYDKLFLMFKGLIQRCNYQYSFGCE 9E5D39FDE6FCE73B if4g1_cerae_protein Eukaryotic translation initiation factor 4 gamma 1 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to Q04637 z3h7b_human Zinc finger CCCH domain-containing protein 7B Rotavirus 'X'-associated non-structural protein ZC3H7B KIAA1031 protein protein human Homo sapiens Human MERQKRKADIEKGLQFIQSTLPLKQEEYEAFLLKLVQNLFAEGNDLFREKDYKQALVQYMEGLNVADYAASDQVALPRELLCKLHVNRAACYFTMGLYEKALEDSEKALGLDSESIRALFRKARALNELGRHKEAYECSSRCSLALPHDESVTQLGQELAQKLGLRVRKAYKRPQELETFSLLSNGTAAGVADQGTSNGLGSIDDIETDCYVDPRGSPALLPSTPTMPLFPHVLDLLAPLDSSRTLPSTDSLDDFSDGDVFGPELDTLLDSLSLVQGGLSGSGVPSELPQLIPVFPGGTPLLPPVVGGSIPVSSPLPPASFGLVMDPSKKLAASVLDALDPPGPTLDPLDLLPYSETRLDALDSFGSTRGSLDKPDSFMEETNSQDHRPPSGAQKPAPSPEPCMPNTALLIKNPLAATHEFKQACQLCYPKTGPRAGDYTYREGLEHKCKRDILLGRLRSSEDQTWKRIRPRPTKTSFVGSYYLCKDMINKQDCKYGDNCTFAYHQEEIDVWTEERKGTLNRDLLFDPLGGVKRGSLTIAKLLKEHQGIFTFLCEICFDSKPRIISKGTKDSPSVCSNLAAKHSFYNNKCLVHIVRSTSLKYSKIRQFQEHFQFDVCRHEVRYGCLREDSCHFAHSFIELKVWLLQQYSGMTHEDIVQESKKYWQQMEAHAGKASSSMGAPRTHGPSTFDLQMKFVCGQCWRNGQVVEPDKDLKYCSAKARHCWTKERRVLLVMSKAKRKWVSVRPLPSIRNFPQQYDLCIHAQNGRKCQYVGNCSFAHSPEERDMWTFMKENKILDMQQTYDMWLKKHNPGKPGEGTPISSREGEKQIQMPTDYADIMMGYHCWLCGKNSNSKKQWQQHIQSEKHKEKVFTSDSDASGWAFRFPMGEFRLCDRLQKGKACPDGDKCRCAHGQEELNEWLDRREVLKQKLAKARKDMLLCPRDDDFGKYNFLLQEDGDLAGATPEAPAAAATATTGE BE3F8AEEC89D096D zc3h7b-s7-1 2 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association Discovery of RoXaN by interaction with NSP3 in a yeast two-hybrid assay. eif4g1-s7-2 2 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association zc3h7b-s7-2 6 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 25 23 physical association physical association Localization of NSP3 and RoXaN I interaction domains by yeast two-hybrid assay. Sequence length of Q00721 is 315, not 313 as stated in the paper, so deletion mutants have been reannoted to sequence as given. 2A zc3h7b-s7-3 6 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 30 28 physical association physical association Localization of NSP3 and RoXaN I interaction domains by yeast two-hybrid assay. Sequence length of Q00721 is 315, not 313 as stated in the paper, so deletion mutants have been reannoted to sequence as given. 2A zc3h7b-s7-4 5 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Cells were lysed 6 h after infection and immunoprecipitated with a Flag-specific MAb, and then NPS3 was detected by immunoblotting with the NSP3-specific MAb ID3 (Fig. 3A). 3A zc3h7b-s7-5 7 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait physical association physical association Transfected and infected cell lysates were immunoprecipitated with either an NSP1- or NSP3-specific MAb, and Flag-RoXaN I:1-387 was detected by immunoblotting using the Flag-specific MAb (Fig. 3B). 3B zc3h7b-s7-6 7 12 unspecified role unspecified role bait bait n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey physical association physical association Cell lysates were immunoprecipitated with an NSP3-specific MAb, and RoXaN I was detected by immunoblotting using a T7tag-specific MAb (Fig. 3C). 3C Q9UGR2 has a length of 993 amino acids, not 977 as given in the paper zc3h7b-s7-7 3 12 unspecified role unspecified role bait bait n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q9UGR2:p.Leu252_Asp254delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain LDD AAA Fri Jul 22 10:51:26 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_leu_asp268-270ala_ala_ala' Wed May 02 16:31:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_asp_asp268-270ala_ala_ala' Fri Feb 01 16:53:27 GMT 2019 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu268_Asp270delinsAlaAlaAla' Tue Jun 30 18:02:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu252_Asp254delinsAlaAlaAla' 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 51 48 physical association physical association GFP-NSP3:150-313 also coimmunoprecipitated with RoXaN I:1-279, but not with RoXaN I:1-255 or RoXaN I:1-173 (Fig. 4, lanes 2, 3, and 4, respectively), suggesting that aa 256 to 279 of RoXaN I are important for the interaction with NSP3. 4 and 7 Q00721 has a length of 315 amino acids, not 313 as given in the paper s7-1 6 10 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 5 zc3h7b-s7-8 1 10 unspecified role unspecified role ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association When NSP3:88-313 was expressed as the bridging protein in combination with DBD-eIF4G I:120-330 and AD-RoXaN I:1-387, the transformed yeast colonies were able to grow in the absence of histidine (Fig. 5, lane 3), demonstrating that NSP3, eIF4G I, and RoXaN I can form a multiprotein complex. Deletion of the NSP3/PABP-binding domain of eIF4G I (eIF4G I:142-548) impaired the formation of this complex, and yeast colonies were unable to grow on selective medium (Fig. 5, lane 6). Sequence length of Q00721 is 315, not 313 as stated in the paper, so deletion mutants have been reannoted to sequence as given. 5 zc3h7b-eif4g1-1 4 9 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association To investigate the presence of a RoXaN I-NSP3-eIF4G I complex during rotavirus infection, COS-7 cells were transfected with T7tag-RoXaN I and infected with rotavirus RF, and the cell lysates were immunoprecipitated with an eIF4G I-specific antiserum (Fig. 6). 6 Interaction only occurs in virally infected cells, even when the human proteins are highly over-expressed. zc3h7b-eif4g1-2 3 10 unspecified role unspecified role ancillary ancillary n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Interaction of RoXaN I with eIF4G I is dependent on the two protein-binding domains of NSP3. 7 Interaction only occurs in virally infected cells, even when the human proteins are highly over-expressed.