IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7. Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7. Plant Mol. Biol. (0167-4412) 2003 imex curation Ziemienowicz A., Haasen D., Staiger D., Merkle T. thomas.merkle@biologie.uni-freiburg.de 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ziemienowicz A., Haasen D., Staiger D., Merkle T. thomas.merkle@biologie.uni-freiburg.de Accepted 2007-DEC-6 by ORCHARD. Plant Mol. Biol. (0167-4412) 2003 orchard Only protein-protein interactions imex curation tnpo1_arath Transportin-1 Importin beta-2 Karyopherin beta-2 F12A24.13 At2g16950 TRN1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAATAVVWQPRDDGLAEICSLLEQQISPSSVVDKSQIWKQLQHFSQFPDFNNYLVFILVRAEGKSVEVRQAAGLLLKNNLRGAYPSMTQENQKYIKSELLPCLGAADRNIRTTVGTIISVIVNIEGVSGWHELLPALVTCLDSNDLNHMDGAMDALSKICEDIPHVLDTEVPGLAERPINIFLPRLLQFFQSPHASLRKLALGSVNQYIIIMPAALYNSLDKYLQGLFVLANDPVPEVRKLVCAAFVHLTEVLPSSIEPHLRNVMEYMLQVNRDPDEEVSLEACEFWSAYCDAQLPPENLKEFLPRLIPVLLENMAYADDDESLLDAEEDESQPDRDQDLKPRFHTSRLHGSEDFDDDDDDSFNVWNLRKCSAAAIDVLSNVFGDEILPALMPLIQKNLSASGDEAWKQREAAVLALGAIAEGCMNGLYPHLSEIVAFLLPLLDDKFPLIRSISCWTLSRFGKYLIQESGNPKGYEQFEKVLMGLLRRLLDTNKRVQEAACSAFATVEEDAAEELVPHLGVILQHLMCAFGKYQRRNLRIVYDAIGTLADSVREELNKPAYLEILMPPLVAKWQQLSNSDKDLFPLLECFTSISQALGVGFAPFAQPVFQRCMDIIQLQQLAKVNPASAGAQYDREFIVCSLDLLSGLAEGLGSGIESLVQQSNLRDLLLNCCIDEAADVRQSAFALMGDLARVFPVYLQPRLLDFLEIASQQLSANLNRENLSVANNACWAIGELAVKVRQEVSPIVAKVVSSLGLILQHGEGVNKALVENSAITLGRLAWIRPDLVAPHMDHFMKPWCMALSMVRDDIEKEDAFRGLCAVVKVNPSGGVSSLVFICQAIASWHEIRSEDVQTEVSQVLNGYKHMLGNSWAECLSALDPPVKERLARYQV 91F5A6D6A66692D0 rnp1_arath Heterogeneous nuclear ribonucleoprotein 1 FCAALL.156 dl3190w At4g14300 RNP1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSDQGKLFVGGISWETDEDKLREHFTNYGEVSQAIVMRDKLTGRPRGFGFVIFSDPSVLDRVLQEKHSIDTREVDVKRAMSREEQQVSGRTGNLNTSRSSGGDAYNKTKKIFVGGLPPTLTDEEFRQYFEVYGPVTDVAIMYDQATNRPRGFGFVSFDSEDAVDSVLHKTFHDLSGKQVEVKRALPKDANPGGGGRSMGGGGSGGYQGYGGNESSYDGRMDSNRFLQHQSVGNGLPSYGSSGYGAGYGNGSNGAGYGAYGGYTGSAGGYGAGATAGYGATNIPGAGYGSSTGVAPRNSWDTPASSGYGNPGYGSGAAHSGYGVPGAAPPTQSPSGYSNQGYGYGGYSGSDSGYGNQAAYGVVGGRPSGGGSNNPGSGGYMGGGYGDGSWRSDPSQGYGGGYNDGQGRQGQ 18143301651FB62F ran1_arath GTP-binding nuclear protein Ran-1 RAN1 Ras-related nuclear protein 1 F28I16.160 At5g20010 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALPNQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDQNLHFVETPALAPPEVHIDIADQQKNEAELLQAAAQPLPDDDDDIFE 672097A226E51A64 nab2_yeast Nuclear polyadenylated RNA-binding protein NAB2 YGL122C NAB2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQEQYTENLKVIVAEKLAGIPNFNEDIKYVAEYIVLLIVNGGTVESVVDELASLFDSVSRDTLANVVQTAFFALEALQQGESAENIVSKIRMMNAQSLGQSDIAQQQQQQQQQQQPDIAQQQPQQQPQQQPQQQPQQQPQQQPQQQPQQQPQQQPQLQPLQPQLGTQNAMQTDAPATPSPISAFSGVVNAAAPPQFAPVDNSQRFTQRGGGAVGKNRRGGRGGNRGGRNNNSTRFNPLAKALGMAGESNMNFTPTKKEGRCRLFPHCPLGRSCPHAHPTKVCNEYPNCPKPPGTCEFLHPNEDEELMKEMERTREEFQKRKADLLAAKRKPVQTGIVLCKFGALCSNPSCPFGHPTPANEDAKVIDLMWCDKNLTCDNPECRKAHSSLSKIKEVKPISQKKAAPPPVEKSLEQCKFGTHCTNKRCKYRHARSHIMCREGANCTRIDCLFGHPINEDCRFGVNCKNIYCLFRHPPGRVLPEKKGAAPNSNVPTNERPFALPENAIIENAPPQTSFTHQEQDTEMN 40335C3D4658DD91 40335C3D4658DD91 rbg7_arath Glycine-rich RNA-binding protein 7 RBG7 AtRBG7 CCR2 GR-RBP7 GRP7 Glycine-rich protein 7 Protein COLD, CIRCADIAN RHYTHM, AND RNA BINDING 2 F2G1.7 At2g21660 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASGDVEYRCFVGGLAWATDDRALETAFAQYGDVIDSKIINDRETGRSRGFGFVTFKDEKAMKDAIEGMNGQDLDGRSITVNEAQSRGSGGGGGHRGGGGGGYRSGGGGGYSGGGGSYGGGGGRREGGGGYSGGGGGYSSRGGGGGSYGGGRREGGGGYGGGEGGGYGGSGGGGGW 3E1025477F9CF4C4 roa1_human Heterogeneous nuclear ribonucleoprotein A1 HNRNPA1 HNRPA1 Helix-destabilizing protein Single-strand RNA-binding protein hnRNP core protein A1 protein protein human Homo sapiens Human MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF DIP protein P09651 original sequence version: 176 A06683571C6C109F trn1-rnp1 1 3 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2 To delineate the transportin-interacting domain of AtRNP1, we assayed the glycine-rich C-terminus without the RRMs (AtRNP1[183-411]; Figure 2A, C), which still interacted strongly with AtTRN1. In contrast, deletion of 75 (AtRNP1[1-336]; Figure 2A, C) or 156 (AtRNP1[1-255]; not shown) amino acid residues from the C-terminus abolished the interaction. trn1-grp7 1 2 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2 In case of AtGRP7, deletion of 24 amino acid residues from the C-terminus had little effect on the interaction with AtTRN1 (not shown), whereas further deletion (AtGRP7[1�116]; Figure 2A, C) considerably diminished it. trn1-hnrnp1_a1 1 2 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association 2 Full-length human hnRNP A1 and Nab2p[159�348] strongly interacted with AtTRN1 trn1-nab2-1 1 2 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2 Full-length human hnRNP A1 and Nab2p[159�348] strongly interacted with AtTRN1 trn1-ran1 1 4 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2 To delineate the transportin-interacting domain of AtRNP1, we assayed the glycine-rich C-terminus without the RRMs (AtRNP1[183-411]; Figure 2A, C), which still interacted strongly with AtTRN1. In contrast, deletion of 75 (AtRNP1[1-336]; Figure 2A, C) or 156 (AtRNP1[1-255]; not shown) amino acid residues from the C-terminus abolished the interaction.