UniProt UniProt http://www.uniprot.org Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. J. Biol. Chem. (0021-9258) 2003 imex curation Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. Proteins were purified by Nickel chromatography prior to Mono Q. claes.gustafsson@mednut.ki.se 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. A high stringency protocol was used which included 0.1% Nonidet-P40 in the wash buffers. claes.gustafsson@mednut.ki.se Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. J. Biol. Chem. (0021-9258) 2003 imex curation Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. Proteins were purified by Nickel chromatography prior to Mono Q. claes.gustafsson@mednut.ki.se 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. claes.gustafsson@mednut.ki.se Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Antibody 8WG16 was used which recognizes the RNA polymerase II CTD repeat YSPTSPS. J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. J. Biol. Chem. (0021-9258) 2003 imex curation Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. Proteins were purified by Nickel chromatography prior to Mono Q. claes.gustafsson@mednut.ki.se 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. A low stringency protocol was used which included 0.01% Nonidet-P40 in the wash buffers. claes.gustafsson@mednut.ki.se Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro. J. Biol. Chem. (0021-9258) 2003 imex curation Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. Proteins were purified by Nickel chromatography prior to Mono Q. claes.gustafsson@mednut.ki.se 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom weight by comassie molecular weight estimation by coomasie staining weight by comassie Spahr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen CO., Hermand D., Makela TP., Holmberg S., Gustafsson CM. Proteins were purified by Nickel chromatography prior to Mono Q. claes.gustafsson@mednut.ki.se Accepted 2007-OCT-25 by JYOTI. J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation rpb4_schpo DNA-directed RNA polymerase II subunit rpb4 rpb4 SPBC337.14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPRAIFEEDAAQLKLGPEFENEDMLTVSEAKILIETVLAQRARETNGEIPMTDVMKKTVAYFNVFARFKTAEATYACERILGNRFHKFERAQLGTLCCEDAEEARTLIPSLANKIDDQNLQGILDELSTLRKFQD 61C98593A01C1E04 tfb3_schpo RNA polymerase II transcription factor B subunit 3 RNA polymerase II transcription factor B 38 kDa subunit RNA polymerase II transcription factor B p38 subunit CDK-activating kinase assembly factor MAT1 homolog RING finger protein pmh1 pmh1 mcr1 tfb3 SPBC776.18c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDDEGARKVDEKCPLCQADRYLNPNMKLLINPECYHKMCESCVDRIFTTGPAQCPTPGCNKILRKAKFREQTFEDAQIEREVDVRKRISRIFNKGQQEFDSLQAYNDYLEEVEILTFNLIYKIDVEETEEKVKQYEKQNRDSIAANSARAAAEARILAQNEILLKRQKQEAREAAIREHQKEKERREQVEQQIIFDLATSGKDPNKIIQLSDSLKKQQENIASSVSNISRSSSILLSDVQQVAEDTTPFSPLAGEKDGSKYFSYSKNTYQDLYLEKVSHEPGRKCGFRIEDCHLRCLYEAFSGIDYDLESLKKLEVAS FF8472C61268565B rad15_schpo General transcription and DNA repair factor IIH helicase subunit XPD rad15 rhp3 SPAC1D4.12 DNA repair helicase RAD3 homolog rad5 DNA repair helicase rad15 RNA polymerase II transcription factor B subunit rad15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKFYIDDLPILFPYPRIYPEQYQYMCDLKHSLDAGGIALLEMPSGTGKTISLLSLIVSYQQHYPEHRKLIYCSRTMSEIDKALAELKRLMAYRTSQLGYEEPFLGLGLTSRKNLCLHPSVRREKNGNVVDARCRSLTAGFVREQRLAGMDVPTCEFHDNLEDLEPHSLISNGVWTLDDITEYGEKTTRCPYFTVRRMLPFCNVIIYSYHYLLDPKIAERVSRELSKDCIVVFDEAHNIDNVCIESLSIDLTESSLRKASKSILSLEQKVNEVKQSDSKKLQDEYQKLVRGLQDANAANDEDQFMANPVLPEDVLKEAVPGNIRRAEHFIAFLKRFVEYLKTRMKVLHVIAETPTSFLQHVKDITFIDKKPLRFCAERLTSLVRALQISLVEDFHSLQQVVAFATLVATYERGFILILEPFETENATVPNPILRFSCLDASIAIKPVFERFRSVIITSGTLSPLDMYPKMLQFNTVMQESYGMSLARNCFLPMVVTRGSDQVAISSKFEARNDPSVVRNYGNILVEFSKITPDGLVAFFPSYLYLESIVSSWQSMGILDEVWKYKLILVETPDPHETTLALETYRAACSNGRGAVLLSVARGKVSEGVDFDHHYGRAVIMFGIPYQYTESRVLKARLEFLRDTYQIREADFLTFDAMRHAAQCLGRVLRGKDDHGIMVLADKRYGRSDKRTKLPKWIQQYITEGATNLSTDMSLALAKKFLRTMAQPFTASDQEGISWWSLDDLLIHQKKALKSAAIEQSKHEDEMDIDVVET A21025C298A28F08 rpb1_schpo DNA-directed RNA polymerase II subunit rpb1 DNA-directed RNA polymerase III largest subunit rpb1 SPBC28F2.12 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSGIQFSPSSVPLRRVEEVQFGILSPEEIRSMSVAKIEFPETMDESGQRPRVGGLLDPRLGTIDRQFKCQTCGETMADCPGHFGHIELAKPVFHIGFLSKIKKILECVCWNCGKLKIDSSNPKFNDTQRYRDPKNRLNAVWNVCKTKMVCDTGLSAGSDNFDLSNPSANMGHGGCGAAQPTIRKDGLRLWGSWKRGKDESDLPEKRLLSPLEVHTIFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPAHIVSEYEQLLQFHVATYMDNEIAGQPQALQKSGRPLKSIRARLKGKEGRLRGNLMGKRVDFSARTVITGDPNLSLDELGVPRSIAKTLTYPETVTPYNIYQLQELVRNGPDEHPGAKYIIRDTGERIDLRYHKRAGDIPLRYGWRVERHIRDGDVVIFNRQPSLHKMSMMGHRIRVMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPVMGIVQDTLAGVRKFSLRDNFLTRNAVMNIMLWVPDWDGILPPPVILKPKVLWTGKQILSLIIPKGINLIRDDDKQSLSNPTDSGMLIENGEIIYGVVDKKTVGASQGGLVHTIWKEKGPEICKGFFNGIQRVVNYWLLHNGFSIGIGDTIADADTMKEVTRTVKEARRQVAECIQDAQHNRLKPEPGMTLRESFEAKVSRILNQARDNAGRSAEHSLKDSNNVKQMVAAGSKGSFINISQMSACVGQQIVEGKRIPFGFKYRTLPHFPKDDDSPESRGFIENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKAMEDVMVRYDGTVRNAMGDIIQFAYGEDGLDATLVEYQVFDSLRLSTKQFEKKYRIDLMEDRSLSLYMENSIENDSSVQDLLDEEYTQLVADRELLCKFIFPKGDARWPLPVNVQRIIQNALQIFHLEAKKPTDLLPSDIINGLNELIAKLTIFRGSDRITRDVQNNATLLFQILLRSKFAVKRVIMEYRLNKVAFEWIMGEVEARFQQAVVSPGEMVGTLAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPSLTIYLMPWIAANMDLAKNVQTQIEHTTLSTVTSATEIHYDPDPQDTVIEEDKDFVEAFFAIPDEEVEENLYKQSPWLLRLELDRAKMLDKKLSMSDVAGKIAESFERDLFTIWSEDNADKLIIRCRIIRDDDRKAEDDDNMIEEDVFLKTIEGHMLESISLRGVPNITRVYMMEHKIVRQIEDGTFERADEWVLETDGINLTEAMTVEGVDATRTYSNSFVEILQILGIEATRSALLKELRNVIEFDGSYVNYRHLALLCDVMTSRGHLMAITRHGINRAETGALMRCSFEETVEILMDAAASGEKDDCKGISENIMLGQLAPMGTGAFDIYLDQDMLMNYSLGTAVPTLAGSGMGTSQLPEGAGTPYERSPMVDSGFVGSPDAAAFSPLVQGGSEGREGFGDYGLLGAASPYKGVQSPGYTSPFSSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS 15A4F0B59E60E570 rpab5_schpo DNA-directed RNA polymerases I, II, and III subunit RPABC5 rpb10 SPAC1B3.12c DNA-directed RNA polymerases I, II, and III 8.3 kDa polypeptide ABC10-beta RPC8 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIIPIRCFSCGKVIGDKWDTYLTLLQEDNTEGEALDKLGLQRYCCRRMILTHVDLIEKLLCYNPLSKQKNL 8B9EEE8FD59A2458 ercc3_schpo General transcription and DNA repair factor IIH helicase subunit XPB ptr8 DNA repair helicase ptr8 Poly(A)+ RNA transport protein 8 RNA polymerase II transcription factor B subunit ercc3 ercc3 SPAC17A5.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLKRKNNAREGTPDEDLEEYSDYSDVDNYGEEDDDSYKPAPRIRINNNKTKAQTTTNSNEARQSGISAMFGQNDFSNLLGLKLDHTARPLWINPIDGRIILEAFSPLAEQAIDFLVTISEPVSRPAFIHEYRITAYSLYAAVSVGLKTEDIIAVLDRLSKTPIPPSIVDFIRACTVSYGKVKLVLKKNRYFIESGDASVLRLLLRDPVIGPLRIDYSTQSSKQKSSKPSNEDNVEDKKDITNDSSKETAEKSSSDELFSAVVGLQEEEDDEDAVHLFEIKHSSVETIKKRCAEIDYPLLEEYDFRNDNINPDLPIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWSNIKPDHIAVFTADHKERFHSEAGVVVSTYSMVANTRNRSYDSQKMMDFLTGREWGFILLDEVHVVPAAMFRRVVTTIAAHTKLGLTATLVREDDKIDDLNFLIGPKMYEANWMDLAQKGHIAKVQCAEVWCAMTTEFYNEYLRENSRKRMLLYIMNPKKFQACQFLIDYHEKRGDKIIVFSDNVYALRAYAIKLGKYFIYGGTPQQERMRILENFQYNELVNTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSSKRQAFLIDQGYAFKVITNLKGMENLPNLAYASKAERLELLQEVLLQNEEAADLDDGEDTSFGSRSLSRAPAKAKRSSGSLSTLAGADNMAYVEYNKSANKQLKKDSKEHHALFRKHLYTKRR 093AC203A3115614 t2ea_schpo Transcription initiation factor IIE subunit alpha tfa1 SPAC458.07 SPAPYUG7.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSNAPEIVQRLIKMIMRAFYETRHIIFMDAILRHSALTDEQTALLMGIPIKECRFIAGKLREDRLLAIQSRTEMKEGQQRQYHTTYFYIDFCSTIDSIKWRMHQLVKTVEDRMRNDFDSKGYVCPFCNKKFSSLDVLSLVTNEGTFACNVCGTELKDDEESAEMMSSQKRLGKLMGQVNGIIDALKRVDEIVVPQNNFQSALEHAVPVSLDTQNLSQQNLSKSNSDVRLSTSSPSITVDFSADKETDEKRERNCDKQVKAAQNILPEWHATSTISGSITRAGAKDAALHSFRTETVNEVQDTKTDITSEKSALDAYYATLRAKQKEESEFMDSENVDDEEDDDFLDVTTATSLQNKSTDYGSVKRKTENLNSDSDIQNKRTKSIEENNSLPPIVSTNGITDGDTEMQESKKNVIINGFNEDDEDDEDEADFEDV F8D4108A7254F249 tfh47_schpo General transcription and DNA repair factor IIH subunit ssl1 ssl1 RNA polymerase II transcription factor B subunit ssl1 Suppressor of stem-loop protein 1 homolog TFIIH basal transcription factor complex p47 subunit tfh47 SPCC1682.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNENQKSFDSDKSESEDEQKNGRVKVRSRKTDDNEGYTWEGEYQRSWDIVQEDAEGSLVGVIAGLIQSGKRKRLLRDTTPLQRGIIRHMVLVLDLSNSMEERDFHHKRFDLQIKYASEFVLEFFEQNPISQLSIIGVMDGIAHRITDLHGNPQSHIQKLKSLRDCSGNFSLQNALEMARASLSHIASHGTREVLIIFGSILSSDPGDIFKTIDALVHDSIRVRIVGLAAEVAICKEICNKTNSSTKNAYGVVISEQHFRELLLESTIPPATDSAKTTDASLVMMGFPSKVVEQLPSLCACHSIPSRGGFHCPRCKAKVCTLPIECPSCSLVLILSTHLARSYHHLFPLKNWSEIPWSANPKSTHCFACQLPFPKPPVSPFDESTSSMRYACPSCKNHFCLDCDVFAHEQLHECYGCQCSGN 4EB838661170AE05 rpb3_schpo DNA-directed RNA polymerase II subunit RPB3 DNA-directed RNA polymerase II 33 kDa polypeptide rpb3 SPCC1442.10c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDSETHITIRNISKNSVDFVLTNTSLAVANSLRRVVLAEIPTVAIDLVEINVNTSVMPDEFLAHRLGMIPLDSSNIDEPPPVGLEYTRNCDCDQYCPKCSVELFLNAKCTGEGTMEIYARDLVVSSNSSLGHPILADPKSRGPLICKLRKEQEISLRCIAKKGIAKEHAKWSPTSAVAFEYDPWNKLQHTDYWFENDADAEWPKSKNADWEEPPREGEPFNFQEEPRRFYMDVESVGSIPPNEIMVQGLRILQEKLAVLVRDLDEEQPTQLSANELNMEENAEMNWSPYQNGEENTW 94F639734B162DDF t2fb_schpo Transcription initiation factor IIF subunit beta tfg2 SPBC1198.13c SPBC660.03c TFIIF-beta TFIIF medium subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSEEKPTVRTEEDDRYEDDAGDLDLGQIGSRVWLVKIPKFLMDKWNSIPEDDAANLGCVRVKNDEIQLLLQNSPENADVPKIYNLRVMNKFVRNSYVFRESETSSSMKSTALVGTVAHECNVSPVINDDYRRVMQKRALAASAPKRKVQMIDDRGGSLLAPGTLGSRSRSTTSFIRNVKPRTGEGLKNSRIPRNELLDILFKCFEDYEYWTLKGLREYVKQPEVYLKEVLDSIAILNKRGPYALKYSLKPEYKGTMDAASVELRNQQASQSESSSIDHTGKNTSPDNPGTNAEEDEDDDGVEMIDVV 23CF468C3F81E101 rpb7_schpo DNA-directed RNA polymerase II subunit rpb7 rpb7 SPACUNK4.06c SPAPYUK71.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPFFLKELSLTISLHPSYFGPRMQDYLKAKLLADVEGTCSGQYGYIICVLDSNTIDIDKGRVVPGQGFAEFEVKYRAVLWRPFRGEVVDAIVTTVNKMGFFANIGPLNVFVSSHLVPPDMKFDPTANPPNYSGEDQVIEKGSNVRLKIVGTRTDATEIFAIATMKEDYLGVL 0AECC46B9499FD33 t2eb_schpo Transcription initiation factor IIE subunit beta tfa2 SPCC1672.08c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSLSDQLSSFKKKVANQPIYAKPQPRQPASPTPTAYLNSNDGHSSAASSPGSYSLKKKRSKTSLVYSQPADSGVGTHYLSQLHYAVEYLKERNEPKTAEEIASYLSTPLTPMLLNLLKKNNRIYYDERNETFTFKPLHNIRSGAGLLAYLDSQKTHVGMSIKELRDGWPNVTVELEELEKQGEVLLLRTRKDGVPKMVWRNDKSCDCHVDKEFQQVWHEIPIPPTLDLASELGKYGLKPTSVDPSTVKRAGHNQTPKQKKPKTRRGKITNTHLNILRDYSSMKP 6FAC4EE32302733D rpab1_schpo DNA-directed RNA polymerases I, II, and III subunit RPABC1 rpb5 SPAC23C4.15 RPC24B protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSAEEKNIVRVFRAWKTAHQLVHDRGYGVSQAELDLTLDQFKAMHCGMGRNLDRTTLSFYAKPSNDSNKGTIYIEFAKEPSVGIKEMRTFVHTLGDHNHKTGILIYANSMTPSAAKIIATVTGQFTIETFQESDLIVNITHHELVPKHILLSPDEKKELLDRYKLRETQLPRIQLADPVARYLGLKRGEVVKIVRRSETSGRYNSYRICA EB74C07B9F048762 rpb2_schpo DNA-directed RNA polymerase II subunit RPB2 DNA-directed RNA polymerase II 138 kDa polypeptide rpb2 SPAC23G3.01 SPAC521.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSYEDYQYNETLTQEDCWTVISSFFEETSLARQQLFSFDEFVQNTMQEIVDDDSTLTLDQYAQHTGAQGDVTRRYEINFGQIYLSRPTMTEADGSTTTMFPQEARLRNLTYSSPLYVDMRKKVMVAADSNVPIGEEEWLVEEEDEEPSKVFIGKIPIMLRSTFCILNGVSDSELYDLNECPYDQGGYFIINGSEKVIIAQERSAANIVQVFKKAAPSPIAYVAEIRSALERGSRLISSMQIKLMARNTENSGQTIRATLPYIRSDIPIVIVFRALGVVPDRDILEHICYDPNDFQMLEMMKPCIEEAFVIQDKDIALDYIGKRGSTTGVTREKRLRYAHDILQKELLPHITTMEGFETRKAFFLGYMIHRMLLCALERREPDDRDHFGKKRLDLAGPLLASLFRMLFRKMTRDVYKYMQKCVETNREFNLTLAVKSNIITNGLRYSLATGNWGDQKRSMVNRVGVSQVLNRYTFASTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGMVCPAETPEGQACGLVKNLSLMSYVSVGSPSAPIIEFLEEWGLETLEDYNPSASPNATKVFVNGVWLGVHRDPAHLTETLRSLRRRLDISAEVSIVRDIREKELRLFTDAGRICRPLFIVDNNPNSERRGELCIRKEHIQQLIEDKDRYDIDPEQRFGWTALVSSGLIEYLDAEEEETVMIAMSPEDLEASRQMQAGYEVKEELDPAQRVKPAPNPHVHAWTHCEIHPAMILGILASIIPFPDHNQSPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRSMEYLKFRELPAGQNAIVAILCYSGYNQEDSIIMNQASIDRGLFRSIFYRTYTDQEKKIGMTVMEEFERPVRSTTLRMKHGTYDKLEDDGLIAPGTRVSGEDIIIGKTAPIPLDHEELGQRTQLHAKRDVSTPLRSTESGIVDQVMVTTNQEGLKFVKVRMRSTRIPQIGDKFASRHGQKGTIGMTYRHEDMPFSAQGIVPDIIINPHAIPSRMTVAHLVECQLSKVSALSGFEGDATPFTDVTVEAVSKLLRSHGFQSRGFEVMYHGHTGRKLVAQVFLGPTYYQRLKHLVDDKIHARARGPVQILTRQPVEGRSRDGGLRFGEMERDCQISHGCSSVLRERLFDCSDAYRVIVCDICGLIAIASYKKDSYECRSCQNRTRFSQVYLPYAAKLLFQELMSMNIAPRLFTKNHK C5D3794A743494CC tfb1_schpo General transcription and DNA repair factor IIH subunit tfb1 tfb1 RNA polymerase II transcription factor B p73 subunit RNA polymerase II transcription factor B 73 kDa subunit RNA polymerase II transcription factor B subunit 1 SPAC16E8.11c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGDRVEALAIFRKKQGVLSIDSRLKWTGEGKTTPSVDIAFDAISNLQTTPASNPKVMIRVFIVVKEGEDPTSLVFHFTGTPNARENCDMITNELRNAIQRQREGSQSKPQGANVDRVNSTNLEKDIDLQESLLTNNPDLLQTFKEAVMKGHLSNEQFWSTRLHLLRAHAVERSQQRGPYNVLSTIKPKTVDNQMKVSLTGQQIHDMFEQHPLLRKVYDKHVPPLAEGEFWSRFFLSKLCKKLRGDRITPMDPSDDIMDKYLKDNEEVTKVSDEPIASHLFDLEGNDQNANVIAELRPDITMRIDKEALPFMKNINQLSERLLEKSLGNSKRFNNENEETYLKESGFHDLEEEASDSKVVLKIKGQDQLLENNFVPSKNQVGLEPLPSLEALQHLYQEDSISLNHIEHDSDSLAEAAMQLTQSMREKHEFETHGTASNLPIDIKNEIVLCHTVTIEFLHQFWNALLNTTFPDKKAMAPLQNALLNSKKRVEAIASQAQEQNVDPKLVYELVSCTLTSIDTSISEYQRRMSYPPA A042EFFA0B4B469D tfb2_schpo General transcription and DNA repair factor IIH subunit tfb2 tfb2 RNA polymerase II transcription factor B subunit 2 SPBC13G1.13 SPBC31F10.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQAEFKSSINDFLEQLPNHARLYQKPAACLAVFRLLPILARQYVMSMLFNPMPVALSDFDLWIKLSSKVYQSESFNKLVRMHIFQFDGQYITLNSEFRKQFITALTGGGNHNSFGVPCTDEDKHLVTVDFLDAYAKETWETILHFMVGTPEAKFPGEGVLSLLKRGGLMSGPKNQLRITRAGFQFLLQDINAQIWTLLLDYLKLSEDTHMDPVQVLHFLFMLGSLDLGRAYSVDFLTDTQQIMLEDLREYGLVYQRKITSKRFYPTRLATGLTTDYRSLHGKQSENDDDKGFIIVETNYRLYAYTSSPLQIAIIGLFANLRARFSNLVVGVITRDSIRRALMNGIAAEQIITYLTTHAHPQMRSNVPLLPPTLVDQIYLWELEKNRLRATPGILFRDFLTDSGFDQAVEYAKELGVLVWDSSLKRMFFITTTGAQPMIAYLKRRAVK 2090A6187E393F40 taf14_schpo Transcription initiation factor TFIID subunit 14 tfg3 taf14 SPAC22H12.02 TBP-associated factor 14 TBP-associated factor 30 kDa Transcription initiation factor TFIIF 30 kDa subunit Transcription factor G 30 kDa subunit SWI/SNF chromatin-remodeling complex subunit tfg3 SWI/SNF complex subunit tfg3 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTTVKRTVRLITDQNVLPGGEAAVLNDQSFPVREWSIKLVCLNPQGEETDASFVDRVTYKLHPTFQNPTRTIRKPPFQIKEQGWGEFEMEIIIYYADKGGEHRFLHYLHFQQEHYHEDIELNINATRPGLLKALTATGEVPGYSDEGEEARKDKRKNESEVGAGKKKAKAKPVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDLYTLPDNLLLLLYSFCAKRVTM 278FAF8B67883D46 278FAF8B67883D46 cgm2_schpo Cyclin mcs2 Mitotic catastrophe suppressor 2 mcs2 SPBP16F5.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSADKFRDSTHYRDWIFTEEDLSKTRAKVNEKFTNIVRERMLEELSLQNKEASLEVLPPTLTVEEELELVNYYSFQLNALSSALSLPTHIRSTAILFFKRFYLINSVMEYSPKIISFTSLFLATKCNDHYISIEQFCKNMPKTTPEEVLEYEFNVCQSLKWDLYVWLPFRPLQGFLLDCQTVLPKVAVEKFYECHDLSKKFLIETLHSDIYFLHSPSIIALGAIYHTNPTICLQYIEAKKIPELQPLIISISANLKATKKFKIEKKKAQDYGRKLYFCMNPLRNKSSALYLKRKAEEESTNNNKWAKKFSTSSNVLDKNPFE 09B8DDB46563727C t2fa_schpo Transcription initiation factor IIF subunit alpha SPCC1620.09c tfg1 TFIIF large subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDSQETKVFENVKQENPDEKKPKVEEPDSQNNASTQSQQKFLDFQLSWCPESERKTTKYHLLKFHSNKAIDPSKSFVPPIKMQRRDPNAPSSSNGEQNAEQGSSSNVNIASIAPYGGAQNMKQNAFKRKTRQVVKVDPQARRLQEEELSPWIMEDFEGKNTWVSTMEGGQSSAYVLFMFSENGFKVIPTDRFYRFNQRNNFQTLSIDEAEAKMNKKTPIPRWFMKKESEENLAEAGSASPMYKLKTVPNARPVTGPRASGSDDELDYDEEFADDEEAPIMEGNEEDNKKLKDKIKKEMLTANLFGEADQDVDLEEENDRQMSREGKKLQRYLKLLEKNLAYESDEEDEDPYASSHDASSEEEVLQEEEELQKREEKLKSRFSANASKTNTPRPLERTPSSVSPVKASSQLQSPNTSIQIRPQEQLINKPGYIILRLSSEKLSRFANDFPRMVPSMGSTETSVGDQVEVVGDTTNVPIDDSNLITEAEVMKALRAGPISIKDLVHLFQRKIRADNRNRLGIQKIIRKVARFENKLLVLKNY D2E94C9192819D2F rpb11_schpo DNA-directed RNA polymerase II subunit RPB11 rpb11 SPAC3A12.07 DNA-directed RNA polymerase II 14.1 kDa polypeptide protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNQPERYELIELMGLPKVTYELDSKSPNAAVVTLEKEDHTLANMLANQLLSDERVLFAGYKVPHPLNHNFILRVQTVEDCSPKQVIVDAAKSLITHLEEIKVNFMREWELKMISVEGVEMEFS 73FAFDF2F468386E tfb4_schpo General transcription and DNA repair factor IIH subunit tfb4 tfb4 RNA polymerase II transcription factor B subunit 4 SPBC30B4.07c pi078 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDEFQTLKQNASWDISEDANDTPSLLVVILDANPASWYSLSKKVPVSKVLADITVFLNAHLAFHHDNRVAVLASHSDKVEYLYPSIAPEQKVAEVDPTKEANTYRKFREVDDLVLSGMKRLMSSTDKVSRKTMISGALSRALAYINQVQNKNTLRSRILIFSLTGDVALQYIPTMNCIFCAQKKNIPINVCNIEGGTLFLEQAADATGGIYLKVDNPKGLLQYLMMSLFPDQNLRKHLNTPNQANVDFRATCFCHKKVLDIGFVCSVCLSIFCEPRVHCSTCHTKFTVEPMSKKLHT 70A88655E58D2D02 crk1_schpo Serine/threonine-protein kinase crk1 Mitotic catastrophe suppressor 6 crk1 mcs6 mop1 SPBC19F8.07 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDIEKSDKWTYVKERKVGEGTYAVVFLGRQKETNRRVAIKKIKVGQFKDGIDISALREIKFLRESRHDNVIELVDVFSTKSNLNIILEFLDSDLEMLIKDKFIVFQPAHIKSWMVMLLRGLHHIHSRFILHRDLKPNNLLISSDGVLKLADFGLSRDFGTPSHMSHQVITRWYRPPELFMGCRSYGTGVDMWSVGCIFAELMLRTPYLPGESDLDQLNVIFRALGTPEPEVIKSMQQLPNYVEMKHIPPPNGGMEALFSAAGHEEIDLLKMMLDYNPYRRPTAQQALEHHYFSALPKPTHPSLLPRKGGEEGIKHVSSDLQRQNNFPMRANIKFV 8AD88A491ACB1671 tfg1-tfg2-2 4 14 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component physical association physical association Fig 2B rpb1-rpb2-1 2 9 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey association association Fig 2C pmh1-mcs2-1 1 22 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig 3A, Table 1 pmh1-mcs2-2 3 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 26 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey association association Fig 3B, Table 1 Ef1a and actin were identified as contaminating proteins. tfb2-tfb1-1 1 12 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait c-terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 25 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey association association Fig 3C, Table 1 tfa1-tfa2-1 4 16 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component physical association physical association Fig 4B