IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu 22-Jan-2008: Contacted by JYOTI. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Accepted 2007-DEC-8 by DAVET. 22-Jan-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 davet SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu 22-Jan-2008: Contacted by JYOTI. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Accepted 2007-DEC-8 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 davet SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu 22-Jan-2008: Contacted by JYOTI. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Accepted 2007-DEC-8 by DAVET. 22-Jan-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 davet SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu 22-Jan-2008: Contacted by JYOTI. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Accepted 2007-DEC-8 by DAVET. 22-Jan-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 davet hfi1_yeast Transcriptional coactivator HFI1/ADA1 YPL254W HFI1 ADA1 SUP110 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAIQSPAPKPLQPTYPAASPASTNAYMKPGLIGSPAVSNHTEPNNGNNETAEPQGPNQRIDLGAMIEELTSLLGKESWTKYAQIISLFILGKLSRKELSNELELVFSPSAASLEKSNTNHHHSLVRLHNQLLLGIFANSLRENPLGRNGNESSWGFGNGSNNPNNKLKRINKHNSQIEVYKKIVMSLPLNDRNRLKMITKEAGKRGFIFCSVFQARLNNIPKIPIVTNPESLKRVKSNNLKTPLEWSQDIMNGFNVPLASESHSLPDTDSFYLRMVGIAREHGLVGTVDARCVELISLALDQYLKNIIEFTIDTVRYRRKKYSDYYDLNESGLYKSVSEMAADKRDAKIKQLDDDKNEDECADEAKSINNGNNSSKDDIGDISMSSITKAGEAVNEELHENRTISLTNEDIYDSLSIFPNLVEPSGSYYALTNLGLVNDDELVDMKSNIDDLPDFLNEKPTFTPLDERNVGTRHELNWLIKGILTED B3ACF3C6101AB541 B3ACF3C6101AB541 spt8_yeast Transcription factor SPT8 L2144 YLR055C SPT8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDEVDDILINNQVVDDEEDDEEMLSGLENDSKQDLEGNDDGGEDEEDDDDDDEDDDDDEDEREDDDEQEDDDGEDDAARMDKTATPTNEHQHDEQKAAAAGAGGAGDSGDAVTKIGSEDVKLSDVDGGVGSREASSSTHEASANGEVYEYYKHMLNAAQIADSYNIYPTAAIPIQTHVNALAVSRGLKYLFLGGSDGYIRKYDLLNTLEGKLSLTILQKHSLAESIQNAGILQSYWENEIPQKKSEMKLSANKTDYEPKVSPVHSLEVQSECLFILSGLQNGGITMQGVRYMEGSIAHYFKGRNGHTQIVNILRLNGQEDRFLSGSWDKRLLEWDLQTGDIVNEFKKSRSELSSLEMRPLYSSVDVSGNVNSGKENENADDDMDSLFGDEDEDEKQDAGNEPVETGDGSNGEENKEQISEESLNIVYDESVFMTSGLNGSVHIWDRRMTQSPALSLERGAGVPPWCLSACWGVDGDHVYAGRRNACVEQFDLKMPSKPIHNLKLPSISGPVSCVKAMPNNKHLLCASRDNIRLYNVEIAVDASNSTTKSSKVPFLIVPGHHGGIISNLYLDPTSRFIISTSGNRGWQGNSTDTTLIYDIDLE DDE88155E7638B46 DDE88155E7638B46 spt7_yeast Transcriptional activator SPT7 YBR0739 YBR081C SPT7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTERIPIKNYQRTNAKALLKLTEKLFNKNFFDLYLTSQQLVVLEYLLSISSEEDKLKAWDYFLKGNIALNVEKSFPLTQEEEHHGAVSPAVDTRSDDVSSQTIKDNNNTNTNTSISNENHVENEIEDKGDNAIANEDNFVNNDESDNVEEDLFKLDLEDLKQQISGTRFIGNLSLKIRYVLWQCAIDYIYCDRNEFGDENDTEYTLLDVEEKEEEEIGKNEKPQNKEGISKFAEDEDYDDEDENYDEDSTDVKNVDDPPKNLDSISSSNIEIDDERRLVLNISISKETLSKLKTNNVEEIMGNWNKIYHSFEYDKETMIKRLKLEESDKMIEKGKKKRSRSDLEAATDEQDRENTNDEPDTNQKLPTPEGSTFSDTGNKRPKQSNLDLTVNLGIENLSLKHLLSSIQQKKSQLGISDYELKHLIMDVRKNRSKWTSDERIGQEELYEACEKVVLELRNYTEHSTPFLNKVSKREAPNYHQIIKKSMDLNTVLKKLKSFQYDSKQEFVDDIMLIWKNCLTYNSDPSHFLRGHAIAMQKKSLQLIRMIPNITIRNRADLEKEIEDMEKDKDYELDEEEEVAGSGRKGLNMGAHMLAKENGKVSEKDSSKTVKDEAPTNDDKLTSVIPEGEKEKDKTASSTVTVHENVNKNEIKENGKNEEQDMVEESSKTEDSSKDADAAKKDTEDGLQDKTAENKEAGENNEEEEDDDDEDEDEDMVDSQSYLLEKDDDRDDLEISVWKTVTAKVRAEICLKRTEYFKNGKLNSDSEAFLKNPQRMKRFDQLFLEYKEQKALESYRQKIEQNSIMKNGFGTVLKQEDDDQLQFHNDHSLNGNEAFEKQPNDIELDDTRFLQEYDISNAIPDIVYEGVNTKTLDKMEDASVDRMLQNGINKQSRFLANKDLGLTPKMNQNITLIQQIRHICHKISLIRMLQSPLSAQNSRSNPNAFLNNHIYNYTIIDDSLDIDPVSQLPTHDYKNNRELIWKFMHKNISKVAMANGFETAHPSAINMLTEIAGDYLSNLIKTLKLHHETNSLNRGTNVEMLQTTLLENGINRPDDLFSYVESEFGKKTKKLQDIKQKLESFLRALLRPTLQELSERNFEDESQSFFTGDFASELTGEDFFGFRELGLEKEFGVLSSSVPLQLLTTQFQTVDGETKVQAKKIQPEESDSIVYKKITKGMLDAGSFWNTLLPLLQKDYERSKAYIAKQSKSSANDKTSMTSTEDNSFALLEEDQFVSKKTATKARLPPTGKISTTYKKKPIASAFILPEEDLENDVKADPTTTVNAKVGAENDGDSSLFLRTPQPLDPLDMDDAFDDTNMGSNSSFSLSLPRLNQ 083B63624669244F 083B63624669244F gcn5_yeast Histone acetyltransferase GCN5 YGR252W GCN5 ADA4 SWI9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTKHQIEEDHLDGATTDPEVKRVKLENNVEEIQPEQAETNKQEGTDKENKGKFEKETERIGGSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEFRVVNNDNTKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGGITYRPFDKREFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAIGYFKKQGFTKEITLDKSIWMGYIKDYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRRKIRTISKSHIVRPGLEQFKDLNNIKPIDPMTIPGLKEAGWTPEMDALAQRPKRGPHDAAIQNILTELQNHAAAWPFLQPVNKEEVPDYYDFIKEPMDLSTMEIKLESNKYQKMEDFIYDARLVFNNCRMYNGENTSYYKYANRLEKFFNNKVKEIPEYSHLID 3200730DDC7EF70D 3200730DDC7EF70D spt3_yeast Protein SPT3 SPT3 YDR392W D9509.12 Positive regulator of Ty transcription protein protein yeast Saccharomyces cerevisiae Baker's yeast MMDKHKYRVEIQQMMFVSGEINDPPVETTSLIEDIVRGQVIEILLQSNKTAHLRGSRSILPEDVIFLIRHDKAKVNRLRTYLSWKDLRKNAKDQDASAGVASGTGNPGAGGEDDLKKAGGGEKDEKDGGNMMKVKKSQIKLPWELQFMFNEHPLENNDDNDDMDEDEREANIVTLKRLKMADDRTRNMTKEEYVHWSDCRQASFTFRKNKRFKDWSGISQLTEGKPHDDVIDILGFLTFEIVCSLTETALKIKQREQVLQTQKDKSQQSSQDNTNFEFASSTLHRKKRLFDGPENVINPLKPRHIEEAWRVLQTIDMRHRALTNFKGGRLSSKPIIM E0F60B61AB155C17 E0F60B61AB155C17 taf12_yeast Transcription initiation factor TFIID subunit 12 TAF12 TAF61 TAF68 YDR145W YD8358.02 TBP-associated factor 12 TBP-associated factor 61 kDa TAFII-61 TAFII-68 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNPENSGVNANNNTGTGNADAITGAQQNMVLQPRQLQEMAAKFRTLLTEARNVGETTPRGKELMFQAAKIKQVYDALTLNRRRQQAAQAYNNTSNSNSSNPASIPTENVPNSSQQQQQQQQQTRNNSNKFSNMIKQVLTPEENQEYEKLWQNFQVRHTSIKEKETYLKQNIDRLEQEINKQTDEGPKQQLQEKKIELLNDWKVLKIEYTKLFNNYQNSKKTFYVECARHNPALHKFLQESTQQQRVQQQRVQQQQQQQQQQQQQQQQQQQQQQQRQGQNQRKISSSNSTEIPSVTGPDALKSQQQQQNTITATNNPRGNVNTSQTEQSKAKVTNVNATASMLNNISSSKSAIFKQTEPAIPISENISTKTPAPVAYRSNRPTITGGSAMNASALNTPATTKLPPYEMDTQRVMSKRKLRELVKTVGIDEGDGETVIDGDVEELLLDLADDFVTNVTAFSCRLAKHRKSDNLEARDIQLHLERNWNIRIPGYSADEIRSTRKWNPSQNYNQKLQSITSDKVAAAKNNGNNVASLNTKK A8FC84E46F8370D3 A8FC84E46F8370D3 tra1_yeast Transcription-associated protein 1 TRA1 p400 kDa component of SAGA YHR099W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLTEQIEQFASRFRDDDATLQSRYSTLSELYDIMELLNSPEDYHFFLQAVIPLLLNQLKEVPISYDAHSPEQKLRNSMLDIFNRCLMNQTFQPYAMEVLEFLLSVLPKENEENGILCMKVLTTLFKSFKSILQDKLDSFIRIIIQIYKNTPNLINQTFYEAGKAEQGDLDSPKEPQADELLDEFSKNDEEKDFPSKQSSTEPRFENSTSSNGLRSSMFSFKILSECPITMVTLYSSYKQLTSTSLPEFTPLIMNLLNIQIKQQQEAREQAESRGEHFTSISTEIINRPAYCDFILAQIKATSFLAYVFIRGYAPEFLQDYVNFVPDLIIRLLQDCPSELSSARKELLHATRHILSTNYKKLFLPKLDYLFDERILIGNGFTMHETLRPLAYSTVADFIHNIRSELQLSEIEKTIKIYTGYLLDESLALTVQIMSAKLLLNLVERILKLGKENPQEAPRAKKLLMIIIDSYMNRFKTLNRQYDTIMKYYGRYETHKKEKAEKLKNSIQDNDKESEEFMRKVLEPSDDDHLMPQPKKEDINDSPDVEMTESDKVVKNDVEMFDIKNYAPILLLPTPTNDPIKDAFYLYRTLMSFLKTIIHDLKVFNPPPNEYTVANPKLWASVSRVFSYEEVIVFKDLFHECIIGLKFFKDHNEKLSPETTKKHFDISMPSLPVSATKDARELMDYLAFMFMQMDNATFNEIIEQELPFVYERMLEDSGLLHVAQSFLTSEITSPNFAGILLRFLKGKLKDLGNVDFNTSNVLIRLFKLSFMSVNLFPNINEVVLLPHLNDLILNSLKYSTTAEEPLVYFYLIRTLFRSIGGGRFENLYRSIKPILQVLLQSLNQMILTARLPHERELYVELCITVPVRLSVLAPYLPFLMKPLVFALQQYPDLVSQGLRTLELCIDNLTAEYFDPIIEPVIDDVSKALFNLLQPQPFNHAISHNVVRILGKLGGRNRQFLKPPTDLTEKTELDIDAIADFKINGMPEDVPLSVTPGIQSALNILQSYKSDIHYRKSAYKYLTCVLLLMTKSSAEFPTNYTELLKTAVNSIKLERIGIEKNFDLEPTVNKRDYSNQENLFLRLLESVFYATSIKELKDDAMDLLNNLLDHFCLLQVNTTLLNKRNYNGTFNIDLKNPNFMLDSSLILDAIPFALSYYIPEVREVGVLAYKRIYEKSCLIYGEELALSHSFIPELAKQFIHLCYDETYYNKRGGVLGIKVLIDNVKSSSVFLKKYQYNLANGLLFVLKDTQSEAPSAITDSAEKLLIDLLSITFADVKEEDLGNKVLENTLTDIVCELSNANPKVRNACQKSLHTISNLTGIPIVKLMDHSKQFLLSPIFAKPLRALPFTMQIGNVDAITFCLSLPNTFLTFNEELFRLLQESIVLADAEDESLSTNIQKTTEYSTSEQLVQLRIACIKLLAIALKNEEFATAQQGNIRIRILAVFFKTMLKTSPEIINTTYEALKGSLAENSKLPKELLQNGLKPLLMNLSDHQKLTVPGLDALSKLLELLIAYFKVEIGRKLLDHLTAWCRVEVLDTLFGQDLAEQMPTKIIVSIINIFHLLPPQADMFLNDLLLKVMLLERKLRLQLDSPFRTPLARYLNRFHNPVTEYFKKNMTLRQLVLFMCNIVQRPEAKELAEDFEKELDNFYDFYISNIPKNQVRVVSFFTNMVDLFNTMVITNGDEWLKKKGNMILKLKDMLNLTLKTIKENSFYIDHLQLNQSIAKFQALYLRFTELSERDQNPLLLDFIDFSFSNGIKASYSLKKFIFHNIIASSNKEKQNNFINDATLFVLSDKCLDARIFVLKNVINSTLIYEVATSGSLKSYLVEDKKPKWLELLHNKIWKNSNAILAYDVLDHHDLFRFELLQLSAIFIKADPEIIAEIKKDIIKFCWNFIKLEDTLIKQSAYLVTSYFISKFDFPIKVVTQVFVALLRSSHVEARYLVKQSLDVLTPVLHERMNAAGTPDTWINWVKRVMVENSSSQNNILYQFLISHPDLFFNSRDLFISNIIHHMNKITFMSNSNSDSHTLAIDLASLILYWENKTLEITNVNNTKTDSDGDVVMSDSKSDINPVEADTTAIIVDANNNSPISLHLREACTAFLIRYVCASNHRAIETELGLRAINILSELISDKHWTNVNVKLVYFEKFLIFQDLDSENILYYCMNALDVLYVFFKNKTKEWIMENLPTIQNLLEKCIKSDHHDVQEALQKVLQVIMKAIKAQGVSVIIEEESPGKTFIQMLTSVITQDLQETSSVTAGVTLAWVLFMNFPDNIVPLLTPLMKTFSKLCKDHLSISQPKDAMALEEARITTKLLEKVLYILSLKVSLLGDSRRPFLSTVALLIDHSMDQNFLRKIVNMSRSWIFNTEIFPTVKEKAAILTKMLAFEIRGEPSLSKLFYEIVLKLFDQEHFNNTEITVRMEQPFLVGTRVEDIGIRKRFMTILDNSLERDIKERLYYVIRDQNWEFIADYPWLNQALQLLYGSFNREKELSLKNIYCLSPPSILQEYLPENAEMVTEVNDLELSNFVKGHIASMQGLCRIISSDFIDSLIEIFYQDPKAIHRAWVTLFPQVYKSIPKNEKYGFVRSIITLLSKPYHTRQISSRTNVINMLLDSISKIESLELPPHLVKYLAISYNAWYQSINILESIQSNTSIDNTKIIEANEDALLELYVNLQEEDMFYGLWRRRAKYTETNIGLSYEQIGLWDKAQQLYEVAQVKARSGALPYSQSEYALWEDNWIQCAEKLQHWDVLTELAKHEGFTDLLLECGWRVADWNSDRDALEQSVKSVMDVPTPRRQMFKTFLALQNFAESRKGDQEVRKLCDEGIQLSLIKWVSLPIRYTPAHKWLLHGFQQYMEFLEATQIYANLHTTTVQNLDSKAQEIKRILQAWRDRLPNTWDDVNMWNDLVTWRQHAFQVINNAYLPLIPALQQSNSNSNINTHAYRGYHEIAWVINRFAHVARKHNMPDVCISQLARIYTLPNIEIQEAFLKLREQAKCHYQNMNELTTGLDVISNTNLVYFGTVQKAEFFTLKGMFLSKLRAYEEANQAFATAVQIDLNLAKAWAQWGFFNDRRLSEEPNNISFASNAISCYLQAAGLYKNSKIRELLCRILWLISIDDASGMLTNAFDSFRGEIPVWYWITFIPQLLTSLSHKEANMVRHILIRIAKSYPQALHFQLRTTKEDFAVIQRQTMAVMGDKPDTNDRNGRRQPWEYLQELNNILKTAYPLLALSLESLVAQINDRFKSTTDEDLFRLINVLLIDGTLNYNRLPFPRKNPKLPENTEKNLVKFSTTLLAPYIRPKFNADFIDNKPDYETYIKRLRYWRRRLENKLDRASKKENLEVLCPHLSNFHHQKFEDIEIPGQYLLNKDNNVHFIKIARFLPTVDFVRGTHSSYRRLMIRGHDGSVHSFAVQYPAVRHSRREERMFQLYRLFNKSLSKNVETRRRSIQFNLPIAIPLSPQVRIMNDSVSFTTLHEIHNEFCKKKGFDPDDIQDFMADKLNAAHDDALPAPDMTILKVEIFNSIQTMFVPSNVLKDHFTSLFTQFEDFWLFRKQFASQYSSFVFMSYMMMINNRTPHKIHVDKTSGNVFTLEMLPSRFPYERVKPLLKNHDLSLPPDSPIFHNNEPVPFRLTPNIQSLIGDSALEGIFAVNLFTISRALIEPDNELNTYLALFIRDEIISWFSNLHRPIIENPQLREMVQTNVDLIIRKVAQLGHLNSTPTVTTQFILDCIGSAVSPRNLARTDVNFMPWF AE3588676F5D5777 AE3588676F5D5777 taf5_yeast Transcription initiation factor TFIID subunit 5 TAF5 TAF90 YBR198C YBR1410 TBP-associated factor 5 TBP-associated factor 90 kDa TAFII-90 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQKQSTNQNQNGTHQPQPVKNQRTNNAAGANSGQQPQQQSQGQSQQQGRSNGPFSASDLNRIVLEYLNKKGYHRTEAMLRAESGRTLTPQNKQSPANTKTGKFPEQSSIPPNPGKTAKPISNPTNLSSKRDAEGGIVSSGRLEGLNAPENYIRAYSMLKNWVDSSLEIYKPELSYIMYPIFIYLFLNLVAKNPVYARRFFDRFSPDFKDFHGSEINRLFSVNSIDHIKENEVASAFQSHKYRITMSKTTLNLLLYFLNENESIGGSLIISVINQHLDPNIVESVTAREKLADGIKVLSDSENGNGKQNLEMNSVPVKLGPFPKDEEFVKEIETELKIKDDQEKQLNQQTAGDNYSGANNRTLLQEYKAMNNEKFKDNTGDDDKDKIKDKIAKDEEKKESELKVDGEKKDSNLSSPARDILPLPPKTALDLKLEIQKVKESRDAIKLDNLQLALPSVCMYTFQNTNKDMSCLDFSDDCRIAAAGFQDSYIKIWSLDGSSLNNPNIALNNNDKDEDPTCKTLVGHSGTVYSTSFSPDNKYLLSGSEDKTVRLWSMDTHTALVSYKGHNHPVWDVSFSPLGHYFATASHDQTARLWSCDHIYPLRIFAGHLNDVDCVSFHPNGCYVFTGSSDKTCRMWDVSTGDSVRLFLGHTAPVISIAVCPDGRWLSTGSEDGIINVWDIGTGKRLKQMRGHGKNAIYSLSYSKEGNVLISGGADHTVRVWDLKKATTEPSAEPDEPFIGYLGDVTASINQDIKEYGRRRTVIPTSDLVASFYTKKTPVFKVKFSRSNLALAGGAFRP B42315B8C752D0B6 B42315B8C752D0B6 ngg1_yeast Chromatin-remodeling complexes subunit NGG1 NGG1 ADA3 YDR176W YD9395.09 SWI7 Transcriptional adapter 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPRHGRRGKLPKGEKLPKKEGGDNTPSKLLSSMLKTLDLTFERDIGMLNGKSVRSIPNKKTLLELQSQLDSLNEILGTIARGDQETIEALRKIRDSKNEKQANDEKQETSNADGQHESSTATEETNIIDKGVQSPPKPPPSNEISGTIENDVESIKQAADNMAKEEINEDKDLQVHRDQPREKRPFDSETENRATENENTQRPDNKKQKIDVDKMENDPTVKNPKSEFVVSQTLPRAAAALGLFNEEGLESTGEDFLKKKYNVASYPTNDLKDLLPGELPDMDFSHPKPTNQIQFNTFLAFVENFFKDLSDDNLKFLKMKYIIPDSLQFDKTYDPEVNPFIIPKLGPLYTDVWFKDENDKNSAYKKPSPYSNDASTILPKKSANELDDNALESGSISCGPLLSRLLSAVLKDDNDKSELQSSKIIRDGGLPRTGGEDDIQSFRNNNNDTVDMTLSQENGPSVQTPDNDIDEEASFQAKLAENKGSNGGTTSTLPQQIGWITNGINLDYPTFEERLKRELKYVGIYMNLPKDENNPNSDDPDWVTGREDDEISAELRELQGTLKQVTKKNQKRKAQLIPLVERQLAWQEYSSILEDLDKQIDQAYVKRIRVPKKRKKHHTAASNNVNTGTTSQIAQQKAANSSLKSLLDKRQRWINKIGPLFDKPEIMKRIPNESVFKDMDQEEDEDEADVFAQNTNKDVELN 489016BFD1C095D4 489016BFD1C095D4 spt20_yeast Transcription factor SPT20 SPT20 ADA5 YOL148C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSANSPTGNDPHVFGIPVNATPSNMGSPGSPVNVPPPMNPAVANVNHPVMRTNSNSNANEGTRTLTREQIQQLQQRQRLLLQQRLLEQQRKQQALQNYEAQFYQMLMTLNKRPKRLYNFVEDADSILKKYEQYLHSFEFHIYENNYKICAPANSRLQQQQKQPELTSDGLILTKNNETLKEFLEYVARGRIPDAIMEVLRDCNIQFYEGNLILQVYDHTNTVDVTPKENKPNLNSSSSPSNNNSTQDNSKIQQPSEPNSGVANTGANTANKKASFKRPRVYRTLLKPNDLTTYYDMMSYADNARFSDSIYQQFESEILTLTKRNLSLSVPLNPYEHRDMLEETAFSEPHWDSEKKSFIHEHRAESTREGTKGVVGHIEERDEFPQHSSNYEQLMLIMNERTTTITNSTFAVSLTKNAMEIASSSSNGVRGASSSTSNSASNTRNNSLANGNQVALAAAAAAAAVGSTMGNDNNQFSRLKFIEQWRINKEKRKQQALSANINPTPFNARISMTAPLTPQQQLLQRQQQALEQQQNGGAMKNANKRSGNNATSNNNNNNNNLDKPKVKRPRKNAKKSESGTPAPKKKRMTKKKQSASSTPSSTTMS 3D67937B65F9AA6A 3D67937B65F9AA6A ada2_yeast Transcriptional adapter 2 ADA2 D9461.33 YDR448W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNKFHCDVCSADCTNRVRVSCAICPEYDLCVPCFSQGSYTGKHRPYHDYRIIETNSYPILCPDWGADEELQLIKGAQTLGLGNWQDIADHIGSRGKEEVKEHYLKYYLESKYYPIPDITQNIHVPQDEFLEQRRHRIESFRERPLEPPRKPMASVPSCHEVQGFMPGRLEFETEFENEAEGPVKDMVFEPDDQPLDIELKFAILDIYNSRLTTRAEKKRLLFENHLMDYRKLQAIDKKRSKEAKELYNRIKPFARVMTAQDFEEFSKDILEELHCRARIQQLQEWRSNGLTTLEAGLKYERDKQARISSFEKFGASTAASLSEGNSRYRSNSAHRSNAEYSQNYSENGGRKKNMTISDIQHAPDYALLSNDEQQLCIQLKILPKPYLVLKEVMFRELLKTGGNLSKSACRELLNIDPIKANRIYDFFQSQNWM 9637E1EDBBED0AC3 9637E1EDBBED0AC3 taf6_yeast Transcription initiation factor TFIID subunit 6 TAF6 TAF60 YGL112C G2985 TBP-associated factor 6 TBP-associated factor 60 kDa protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTQQQSYTIWSPQDTVKDVAESLGLENINDDVLKALAMDVEYRILEIIEQAVKFKRHSKRDVLTTDDVSKALRVLNVEPLYGYYDGSEVNKAVSFSKVNTSGGQSVYYLDEEEVDFDRLINEPLPQVPRLPTFTTHWLAVEGVQPAIIQNPNLNDIRVSQPPFIRGAIVTALNDNSLQTPVTSTTASASVTDTGASQHLSNVKPGQNTEVKPLVKHVLSKELQIYFNKVISTLTAKSQADEAAQHMKQAALTSLRTDSGLHQLVPYFIQFIAEQITQNLSDLQLLTTILEMIYSLLSNTSIFLDPYIHSLMPSILTLLLAKKLGGSPKDDSPQEIHEFLERTNALRDFAASLLDYVLKKFPQAYKSLKPRVTRTLLKTFLDINRVFGTYYGCLKGVSVLEGESIRFFLGNLNNWARLVFNESGITLDNIEEHLNDDSNPTRTKFTKEETQILVDTVISALLVLKKDLPDLYEGKGEKVTDEDKEKLLERCGVTIGFHILKRDDAKELISAIFFGE 14B590E02C16F289 14B590E02C16F289 ada2-ada3-1 2 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey association association 1 B ada2-ada3-5 2 7 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey association association 1 B SALSA, as compared to SAGA, lacks Spt8 and also contains a truncated form of Spt7. ada2-ada3-6 3 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1 B SALSA, as compared to SAGA, lacks Spt8 and also contains a truncated form of Spt7. ada2-ada3-7 3 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association 1 B spt3-spt8 4 14 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association 4 These SAGA proteins were abundant in fractions 38-39, whereas a very small amount of SALSA Spt20 and Spt3 were present about seven fractions earlier. However, in a spt7-217 strain the SALSA subunits were predominant. spt3-spt8-1 1 9 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association 4 These SAGA proteins were not abundant, whereas SALSA Spt20 and Spt3 were present about seven fractions earlier were abundant. However, in a strain with wild-type Spt7 the SAGA subunits were predominant. spt3-spt20 4 14 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association 4 The SAGA proteins were abundant in fractions 38-39, whereas a very small amount of the SALSA Spt20 and Spt3 were present about seven fractions earlier. However, in a spt7-217 strain the SALSA subunits were predominant. spt3-spt20-1 1 14 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association 4 The SAGA proteins were not abundant, whereas SALSA Spt20 and Spt3 were present about seven fractions earlier were abundant. However, in a strain with wild-type Spt7 the SAGA subunits were predominant. ada2-ada3-8 3 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5 A These proteins apart Spt8 are coimmunoprecipitated with Ada-2-2FLAG in cells expressing Spt7Delta213C (aa 1- 1119) (like spt7-217), indicating a structural and functional relationship between the Spt7 C-terminus and the participitation of Spt8 in the complex. ada2-ada3-9 3 7 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey association association 5 A Spt8 is not coimmunoprecipitated with Ada-2-2FLAG in cells expressing Spt7Delta213C (aa 1- 1119) (like spt7-217), indicating a structural and functional relationship between the Spt7 C-terminus and the participitation of Spt8 in the complex. ada2-ada3-10 3 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5 A These proteins were coimmunoprecipitated from extracts of cells expressing Spt7Delta400C (aa 1-932) or Spt7 deletion mutants. spt3-ada3 3 16 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5 B spt20-ada1 3 13 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5 B spt8-ada1 3 16 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association SAGA/SALSA complex proteins could be pulled down by Spt8 in the presence of wild-type Spt7 but not Delta213C Spt7 (aa 1-1119). 5 B