IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Intra- and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays. Intra- and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays. Structure (0969-2126) 2002 imex curation Reinders A., Schulze W., Thaminy S., Stagljar I., Frommer WB., Ward JM. BNPAGE analysis of the SUT1 reconstituted transporter (Figure 6), was not curated since the authors do not show the presence of the N terminal region of SUT1 in the complex but assume it is present. frommer@zmbp.uni-tuebingen.de 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Reinders A., Schulze W., Thaminy S., Stagljar I., Frommer WB., Ward JM. BNPAGE analysis of the SUT1 reconstituted transporter (Figure 6), was not curated since the authors do not show the presence of the N terminal region of SUT1 in the complex but assume it is present. frommer@zmbp.uni-tuebingen.de Accepted 2007-DEC-14 by JYOTI. Structure (0969-2126) 2002 Only protein-protein interactions imex curation q40167_sollc LeSUT1 protein protein sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato MENGTKGKLTVSSSLQIEQPLPPSKLWKIIVVASIAAGVQFGWALQLSLLTPYVQLLGIPHRFASFIWLCGPISGMIVQPVVGYYSDNCSSRFGRRRPFIAAGAALVTIAVFLIGFAADLGHASGDPLGKGSKPRAIAVFVVGFWILDVANNMLQGPCRALLADLSGGKSGKMRTANAFFSFFKAVGNILGYAAGSYSRLFKVFPFSKTKACDMYRANLKSCFFIAIFLLLSLTTLALTLVRENELPEKEELEIDEKLSGAGKSKVPFFGEIFGALKDLPRPMWILLLVTCLNWIAWFPFFLYDTDWMAKEVFGGQVGDAKLYDLGVRAGALGLLLQSVVLGFMSLGVEFLGKKIGGAKRLWGILNFVLAICLAMTILVTKMAEKSRGHDAAGTLMGPTPGVKIGALLLFAALGIPLAVTFSIPFALAS 62024DC53BC72B1C q43653_soltu SUT1 protein protein soltu Solanum tuberosum (Potato) Potato MENGTKREGLGKLTVSSSLQVEQPLAPSKLWKIIVVASIAAGVQFGWALQLSLLTPYVQLLGIPHKFASFIWLCGPISGMIVQPVVGYYSDNCSSRFGRRRPFIAAGAALVMIAVFLIGFAADLGHASGDTLGKGFKPRAIAVFVVGFWILDVANNMLQGPCRALLADLSGGKSGRMRTANAFFSFFMAVGNILGYAAGSYSHLFKVFPFSKTKACDMYCANLKSCFFIAIFLLLSLTTIALTLVRENELPEKDEQEIDEKLAGAGKSKVPFFGEIFGALKELPRPMWILLLVTCLNWIAWFPFFLYDTDWMAKEVFGGQVGDARLYDLGVRAGAMGLLLQSVVLGFMSLGVEFLGKKIGGAKRLWGILNFVLAICLAMTILVTKMAEKSRQHDPAGTLMGPTPGVKIGALLLFAALGIPLAATFSIPFALASIFSSNRGSGQGLSLGVLNLAIVVPQMLVSLVGGPWDDLFGGGNLPGFVVGAVAAAASAVLALTMLPSPPADAKPAVAMGLSIK 4FD06C095E49A377 sut1 1 3 unspecified role unspecified role prey prey n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 3 The results indicate a physical interaction between StSUT1-I and StSUT1-II sut1-1 1 2 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1552101]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 510-510, sequence length 429 [EBI-1552101]183-510 n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3 Comparable results were obtained when using the orthologous protein from tomato, LeSUT1 (Figures 1 and 3B). Interestingly, the N-terminal half of LeSUT1 not only interacted with the C-terminal half of StSUT1, but also with a C-terminal fragment encompassing membrane spans V�XII (LeSUT1-IIV�XII) The length of the UniProt protein Q40167 is 429 amino acids, Reinders et al state that the protein used in their study is 510 amino acids in length. sut1-sut1 1 2 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role prey prey c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain n-terminal CubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 8 the N-terminal half of LeSUT2 was fused to CubPLV. LeSUT2-I-CubPLV interacted with the second half of StSUT1