IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins. Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins. J. Exp. Bot. (0022-0957) 2002 Kim BH., Schoffl F. friedrich.scheoffl@zmbp.uni-tubingen.de orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim BH., Schoffl F. friedrich.scheoffl@zmbp.uni-tubingen.de Accepted 2007-DEC-6 by ORCHARD. J. Exp. Bot. (0022-0957) 2002 orchard hfa1a_arath Heat stress transcription factor A-1a HSF1 dl4910c FCAALL.107 HSFA1A At4g17750 Heat shock factor protein 1 Heat shock transcription factor 1 HSF13 AtHsf-13 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFVNFKYFSFFIRTKMDGVTGGGTNIGEAVTAPPPRNPHPATLLNANSLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQIMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLREDSTAATESNSHSHSLEASDGQIVKYQPLRNDSMMWNMMKTDDKYPFLDGFSSPNQVSGVTLQEVLPITSGQSQAYASVPSGQPLSYLPSTSTSLPDTIMPETSQIPQLTRESINDFPTENFMDTEKNVPEAFISPSPFLDGGSVPIQLEGIPEDPEIDELMSNFEFLEEYMPESPVFGDATTLENNNNNNNNNNNNNNNNNNNNTNGRHMDKLIEELGLLTSETEH 2F0DE55252B5682A hs704_arath Heat shock 70 kDa protein 4 HSP70-4 HSC70-4 HSP70 Heat shock cognate 70 kDa protein 4 Heat shock cognate protein 70-4 Heat shock protein 70-4 MED37C MED37_2 T16H11.7 T2E22.11 At3g12580 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPTNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVSGPGEKPMIVVNHKGEEKQFSAEEISSMVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLFEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMVQEAEKYKAEDEEHKKKVDAKNALENYAYNMRNTIKDEKIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEFEDKMKELESLCNPIIARMYQGAGPDMGGAGGMDDDTPAGGSGGAGPKIEEVD 33381E896461C5B9 hs701_arath Heat shock 70 kDa protein 1 HSP70-1 ERD2 HSC70-1 Heat shock cognate 70 kDa protein 1 Heat shock cognate protein 70-1 Heat shock protein 70-1 MED37E MED37_4 Protein EARLY-RESPONSIVE TO DEHYDRATION 2 T22P11.90 At5g02500 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQAGPADKPMIYVEYKGEEKEFAAEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMDDDAPPASGGAGPKIEEVD BD689BA0C936A420 hsf1-hsp70 1 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 2 However, the interaction between full-length HSF and HSP70/HSC70 resulted in much higher levels of �-galactosidase activity The authors concluded that both N-terminal (1-146) and C-terminal (421-491) regions of the HSF1 protein were required for the interaction. hsf1-hsc70 1 4 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait c-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 2 However, the interaction between full-length HSF and HSP70/HSC70 resulted in much higher levels of �-galactosidase activity The authors concluded that both N-terminal (1-146) and C-terminal (421-491) regions of the HSF1 protein were required for the interaction.