UniProt UniProt http://www.uniprot.org The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. J. Biol. Chem. (0021-9258) 2002 Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca 22-Jan-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca Accepted 2007-Not a month!-3 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. cDNA library ATCC 87289 J. Biol. Chem. (0021-9258) 2002 orchard The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. J. Biol. Chem. (0021-9258) 2002 Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca 22-Jan-2008: Contacted by JYOTI. orchard schpm Schizosaccharomyces pombe (fission yeast) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca Accepted 2007-Not a month!-3 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2002 orchard The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. J. Biol. Chem. (0021-9258) 2002 Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca 22-Jan-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca Accepted 2007-Not a month!-3 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2002 orchard The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein. J. Biol. Chem. (0021-9258) 2002 Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca 22-Jan-2008: Contacted by JYOTI. orchard schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Taricani L., Tejada ML., Young PG. youngpg@biology.queensu.ca Accepted 2007-Not a month!-3 by ORCHARD. J. Biol. Chem. (0021-9258) 2002 orchard nu211_schpo Nucleoporin nup211 Nuclear pore protein nup211 nup211 SPCC162.08c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MHDSSWTEADILGVCSFLDIPKTKIDPLLQVDAFTSILIPLISKSKDYESIKNDRIVTEVNYEQQLRNSEKKLLQSNERYDLLEDERKLLENELSQIKEYLREKSSSYDTVLHDCSSLKSVNEALKQAQDQNLKQTAQLQNLLSDKEKEVEKKITIIKDLKDALASSTHQVLELQHTQQEKASLQTNYEFELQKLTQKNSILENNNTWLSRELQGVNDKLLSLHQEASLEKSQLSSQLSDAVLEKDALQRKVSSLSQQFTESNLRYQNIVAELSEMRKQYEFSQVSFEKEISSQKQISELWMEKCEDCSLRLKELQNSNGELEKLLEAAQSSFEEQLESHKEAEASLKSQINFLEKEVSSLESQLKLANERLRHYDEIEISDMSELKYSNLLNNSMKGFKGQSSVSDLYSERLYYKQKYEQTCQEVERLQRSYNHVMEEANLQHPLVKEQFKRFAHMQREIVAMSEQYQKSLEDCQKAKSRYEQLETLFKDKCTENKHYEQETKDLARQVQVLLHELDLCENGIVLGVDSRKKINSYVEKSLTEDETDTDQIISSRLVVFRNIRELQQQNQNLLSAVHELADRMEKDEKPDLDGAEIQEETLIKANETIDQLTKMLEEVSDQLRYSLKERDFFRSLVQENEKLLDMAPATPNSKLNTNLIEQTSYQRSLIRLEQLTNELESLKSISRNKEKKFEEAISSLQLEKSNIQLQLTSLTSERSLALEKLNDLEKSLVLSERSKDELDESYKSLQEQLASKKIEVQNVSSQLSICNSQLEQSNHIVDNLKSENLLLTSVKDKLKADLSNLESKLSSLQQDNFHMKAQIESSNQEYTATVDSMNSRILELSNDLRVANSKLSECSDDVRRLTLQNSFDLREHQTLVLQLQSNITELKQDITLQRTVRNQLEIQTTELKERLKFMEERQENLQSKLIAANKDTTQNPDNVEVEAISIELERTKEKLRMAELEKSNIQQKYLASEKTLEMMNETHEQFKHLVESEISTREEKITSLRSELLDLNKRVEVLKEEKESSSKELAKQLEDAVREKDSALSFKKDYEKIRSDADRVITSLKEDIEKERSLMKECHSNYESEIVSHGRTTQKLRDLRTEFDEVNTKYLKLKANFEQQHSGLSGAEKDWNIQRKAMEDEISSLKDYILGLENQNKLLHSQFDSLSQQITVLQQNSSENLNISANLEAVQDNDLRELVSYLRHEKEIMDNKYELTILDNRGLNQQVKSLQSTVDSLQLELNRLQSLPVSNDQTDTPIISGSQEVQLLYESNSVLRKDNDAKLGKIQELEKEVEKLNASLNPLQTEINELKAEIGAKTASLNLMKEYNSRWKLRFQSVLNKYERVDPTQLEELKKNCEALEKEKQELETKLQETAKETDTFKQQVNSLNEEVENLKKEVEQANTKNTRLAAAWNEKCENLKKSSLTRFAHLKQELTNKNKELTSKNAENEAMQKEIESLKDSNHQLQESASSDAEQITKEQFEQLKSEKERTEKELADSKNELEHLQSEAVDADGKTEISNLEKEIHELRSDKEGLVQQVQNLSAELAALREHSPTQGSLENADEIARLRSQLESTKQYYEKEKETEILAARSELVAEKEKTKEELENQLNEKSQRIKELEEQAQKNSSENTHDNIDDMIKQQVEEKLKENSANFDVKLKKVVAETEFRSKAKISVYEKKTRDLQNKITQLEETIENLNKQLSNPEKTDESTSSVTETKPVTSKPTASKADVGQNATEASSAKREPSGKSLSARLQGTGKQKGVQRPAVSRPVPMKPDSGKLSITGASKRIATSKNAAQNAKELSSTAKSGSLKRQRDDANKGGSSSNQKKAK 1574A467D781E442 rlf2_schpo Chromatin assembly factor 1 subunit rlf2 rlf2 SPBC29A10.03c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNSESVDSDVAASTSNKGNELCSSSTDITSLSVSSPNESVIHSSHSASEADEYVCKLSYEGNRKKRIYNGSAEAGKEKKLQKQRAQEERIRQKEAERLKREKERQQREQEKKLREQEKIAAKKMKELEKLEKERIRLQEQQRRKEERDQKLREKEEAQRLRQEQILNKERQQLKLNNFFTKGVEKRIAPNENFVADKTDELNEFEKEFRPFFIKHQMSLSKYPSPNESDSFLDEVLSTSKSYPLKLNDIFTPSDAVSSANSLGVSNRNSENEVRQLMSAYQDPSVSKPQEILSCLSQIPIKFIFFYQDVRPPYFGSYTKTHSHGSNVLLNPWLEDEDIDYTYDSEAEWVADEEDDGEDLESEDEEVDNSDDIVEDGDNAFVDDEDDDKDSVNASNTHRSSGPLEVIVEGPVWDSKFLPDFNCLSLIEPISSFSASTYLQIDPKEDLWASQDTAPASSGMTIGPTSSLSDDLQVRFPSEDIPKFIEYVRNSHDNKVFLIENLRHMFPYVTKNIISETLGKVAVRKGKSVSDGWIIKENFASLLSS B7413523836A9C4A B7413523836A9C4A imb4_schpo Probable importin subunit beta-4 Karyopherin subunit beta-4 Karyopherin-123 Importin-123 kap123 SPBC14F5.03c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDAQFTLELTQLLFQSIAPDTTQITEATRALETKYLKEPGSLLSLFHIMGTCENPQVRQLAAIEARKLCHKYWSSVDADVQNQIRSNLLDITLKEPESIVRHAFGRVIAALAKLDLPEGKWNELSAFLVQATMDQNDSIREMAVYVLYSIAETVDLDNKLLLDFVNLFSQTITDSSRTVRVTSVQGLGAIAEVLESDDKKLLHAYRATLPGMLLVLQDVVQVGDVDASKQVFDVFNTFLIASGAIISKALGNIIEIITGIANSKQVDDEIRCMALSFIISCIRFKSRKLQALKLGKPLVLTLMEVATEETTDDIDEDCPARLALRSIDLLSTHLSPSQVFYPMFEAACAFSQSPQASYRKAALLSIGVAVEGSSESVAGNLPNIFPIIINGLCDNDMDVRQAALLALSQIAVEIPTEVSKHHAQLLPLVFELMSTQGVKVGKSACNCIDALLEGLDKSEISGYLPMLMERLVGLLEFSDTPDIKSCVAAAIGSAAFAAQDDFIPYFERTMASLSQCLHTTDDDEGYELRGTVMDTLGAIANAVGKQAFLPYTEQLIQLAYEGIQIDHSRLRECSFCFYAVLARVYKEEFAPFLEHIVPALFKSIDQDESDILSERIGAPTAEEISQLLDSVETNEEENDEELEKAMGVNSAIAMEKEIAADALGEICMYVGAPFTPYLEPTVEKLVACTTHFYEGVRKSALSSLWRCATTYYKVCNVPQWQPGLPLKVPVPDTVKNIFEAVRKCTFDTLEEEYEKTVATDILRNFAESIKTCGPVVLGDDYEKLCEVVMEVLQKQHIVQAGDVFDDDFEEEDIVSNEEVDDTEQDALLIDSACDVVIALAVALGGSFADSFKVFYPQIVKYYMSKNGNERAMAVACVGEVAGGIESAITPFTRDVFSLFMAALEDSEGEVRSNAAYSMGLLCQFSTEDLSSEYLNILQKLQPFFTQEVFRTALDNAIGCISRLILHNQNAIPVDQVLPIVFSKLPLKEDYLENAPLYHMILALYRQQNPCLVQHLGELIPVFASVLTGSPEQLNDELRSELLSMVKEIAPQYESVVSNYPQLVALLQ 0D90A3CB4DE13BB6 0D90A3CB4DE13BB6 rs6a_schpo 40S ribosomal protein S6-A rps601 rps6 rps6a SPAC13G6.07c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKLNISYPANGTQKLIEIDDDRRLRVFMEKRMGQEVPGDSVGPEFAGYVFKITGGNDKQGFPMFQGVLLPHRVRLLLRAGHPCYRPRRDGERKRKSVRGCIVGQDLAVLALAIIKQGEQDIPGLTDVTVPKRLGPKRASKIRRFFNLSKEDDVRQFVIRREVVPKKEGKKPYTKAPKIQRLVTPRTLQHKRHRFALKRRQAEKNREEAAEFAQLMAKRVAEAKQKREVVKARRASSLKK 3C65751DFA2D9E16 cbpy_schpo Carboxypeptidase Y cpy1 pcy1 SPAC19G12.10c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLMKQTFLYFLLTCVVSAQFNGYVPPEQNGGDIVVPKDFYEKFGEDFIREQEESSAPLMNPVPERDEAEAPHHPKGHHEFNDDFEDDTALEHPGFKDKLDSFLQPARDFLHTVSDRLDNIFDDDEDEHVREKRPHDSADEDAPRRKHGKCKGKGKHHKGKHAKGKGKKSHPKPEDDSVFFDDERPKHHEFDDEDREFPAHHEPGEHMPPPPMHHKPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPFKHHELEEHEGPEHHRGPEDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHKGPKDKEHHQGPKEKHNERPEQNMQSSHELLVIEAFADLINSVPVEEIAEEFSRFLDTLGIEYYGNIPVHIQENAPKDSSIPPLFEFDDDLELSDLTPEQFAYLEMLKAEGIDPMTAFRDQSHPAKPSNAQPADSSRPYAVFSQEENGEHVNLKAFPDHTLRVKDSKPESLGIDTVKQYTGYLDVEDDRHLFFWFFESRNDPENDPVVLWLNGGPGCSSLTGLFMELGPSSINIETLKPEYNPHSWNSNASVIFLDQPINTGFSNGDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAGESYAGHYIPQFAKEIMEHNQGANFFVASGYEMEKQYINLKSVLIGNGLTDPLVQYYFYGKMACESPYGPIMSQEECDRITGAYDTCAKLITGCYQTGFTPVCIGASLYCNNAMIGPFTKTGLNIYDIREECRDQEHLCYPETGAIESYLNQEFVQEALGVEYDYKGCNTEVNIGFLFKGDWMRKTFRDDVTAILEAGLPVLIYAGDADYICNYMGNEAWTDALEWAGQREFYEAELKPWSPNGKEAGRGKSFKNFGYLRLYEAGHMVPFNQPEASLEMLNSWIDGSLFA 4A8D81CFDAB2D854 abp1_schpo ARS-binding protein 1 abp1 SPBC1105.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGKIKRRAITEHEKRALRHYFFQLQNRSGQQDLIEWFREKFGKDISQPSVSQILSSKYSYLDNTVEKPWDVKRNRPPKYPLLEAALFEWQVQQGDDATLSGETIKRAAAILWHKIPEYQDQPVPNFSNGWLEGFRKRHILHAINEQPTESVVLNNTEPPNDPLSRVYDVTRLTNINDIFTMQETGLFWKLVPNGTPEVEDIKGITRFKARITLTVCCNASGTERLPLWVIGYSQSPRVFRAANVKPEVMNFKWRSNGKASMTTAIMEEWLRWFDACMEGRKVILLIDSYTPHLRAVENIRNSGNDLRNTTVITLPSTSASISQPCSEGVIYALKACYRKHWVQYILEQNELGRNPYNTTNVLRAILWLVKAWTTDISPEIIENAFNLSGVLGLFNESAVTSRALDEMIHPLRELVSEFSVQAAMRIEDFISPSEENIVDSSEDIINQIASQYMDDRAFETDEEESTEFQITTKDAMKAIELLLNYEAQQPDGNPAITISLLNYQKLLEARGGNVNLSRLRST 8D50078EA9E2233F cet1_schpo mRNA-capping enzyme subunit beta mRNA 5'-phosphatase mRNA 5'-triphosphate monophosphatase SPAC644.04 pct1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDLKGLLHEENELPSIEKRKIDENAVEHDKVERKRTKSVAVPKIEMNFLNKPIVPDTTKVISNFLTHYLITEPVEHVEIEAKLGTLIDLETQNRFEFPVMNETILNPEFNLRTRFESDMTASEHKYLNEFLNQAFRDSQKPGRLPFAYKHTKQVDLFYETEDNSRDKIRVSKNQSDNQVLACVKKRRVADLFLYCPNDAFDIRISISDELPVSMPSGNQQPSLTRLKDRVGYVHQEIKIDLTKTTQNDPVYDTTERHELEVEFGNIADLRDRAQKAKDGMEAPLFRRVQLFMDNVRILRREHS 51A5A112CE927AED nuf2_schpo Kinetochore protein nuf2 NMS complex subunit nuf2 nuf2 SPAC27F1.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MARKHTFPSLKRAEILECIDGLGIPFTAKELDQPTSKAVIPLYEEFLDLFMGLTRQNLEEKVNSLQDSVENFEIIHESLRFTVFYQILSQFMQNICFHDFTIQDLLKPDRNRLQLILSAVINFAKLREERLQQFDDDIQKRESLLETYTLLDAQRKDLEEKVLLSQDRKLESEAIIKQNEERNEEMFQSLIEDKRLCSQVRTEYDRIRMEASELKIRYHNVDSLMASTLEEIEKLQSSIVHSPEKLKGKIADTSLRIQNDRSQQVELDKKSKILHTKLNSLQLIEGDLNACLKVLEECLVELDKLEHATVLLSTNQELCDQIEINKKKLEFRKEQLLKQLSNAQEKLEHEQHSRNQKLEAAKQRMDNIREEYKVITQERNKKIQETEKKNAMIEMTEQKIAGMREELESQISSITMEFEKLKSHVELYIAELLRNLRSSNS D716FE83223BD16E D716FE83223BD16E reb1_schpo DNA-binding protein reb1 reb1 SPBC1198.11c SPBC660.01c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDTSVLNPELQIHGFIGVDSLQSSRKRKNDFDDFPLNKGLKTNNNDYSGSIEPKFSPALSIKEDGKNDRNFEALMSLQAQDSNLSEQNTSIHLDALASSSIALGNDNVDSSAFLSKVNKGVNAMRNSTSNQTNDSILISPSEITNMDPFLKGSARWTAEHWDYLERRMQNFCQTYSLDHTQVADSLHEKRLHGPLSSLVKLLVQEMPSFTRRTILRHLRALYNIPGYEKYSRKNSSGRGDFGVQETAIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYSNLYKKLSHRDAKSIYHHVRRAYNPFEDRCVWSKEEDEELRKNVVEHGKCWTKIGRKMARMPNDCRDRWRDVVRFGDKLKRNAWSLEEETQLLQIVAELRNREDLSSDINWTLVAQMLGTRTRLQCRYKFQQLTKAASKFELQENVWLLERIYDSLLNNGGKIHWENIVKEANGRWTRDQMLFQFINLKKMIPSYDNLPLLEATKSAIDDFKVVLSGFSN 667C45F453E4F916 rpn6_schpo Probable 26S proteasome regulatory subunit rpn6 rpn6 SPAC23G3.11 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSKSSLELANNAVKSNDIEKAIDLYKEVLNKGVSKDEKVANEQEQALTNLSDLYVRENRHNDLAQLVQQSRPLMANFSKAKSAKIVRTLIDKFSGEKKSLPLQIEVANDCIKWAIKEKRTFLRQALETKLISLYYDNSSYTDAINLINTLLSELKRMDDKMLLTEVHLLESKVYHAIRNIPKARASLTAAKTSANAVYCPPMLQGNLDLQSGILHADDMDFKTAYSYFYEAYEGFTSLDDDKKALSSLKYMLLSQIMLNSVSEVKSLLTGKHAIRYAGRDIEAMRAIAQAHENRSLADFEKALQDYKPELASDPIIRSHLSSLYDNLLEQNLLRVVEPFSRVEVSHIAELIGLSTVQVEGKLSQMILDKIFYGILDQGSGCLIVYDEPQQDKTYEAALEVIKNMGTVVDLLIENKASALL A2FF8429385D9CE1 snx4_schpo Sorting nexin-4 Autophagy-related protein 24 snx4 atg24 SPAC6F6.12 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSDSVNLDEPSTNSTHFLQCLVTEPRKELQGSRDTHVSYLIITKTNLSIFTRAECKVRRRFSDFVKLQEILSRMNEDCVVPPLPAKHKLEYIKGGRFSDNFINRRAKLLNRYITRCALHPVLHQSPHFIAFLENPNWNNYVRFFIQPKLNNTSKLDEISDSLLNAFSKLKEEPTEFDIQRDHVQQFMFGISNLEGSIQKLLRLEKALESDYEDVSIQFDRLASLDQALDVPIESIQNALQQTGTEYANLTEKLTLLLDTIKDVESYAHSLKELLKRRDQKQQDVEALQEYSAKLSLERDKISSGGSNGFSLSKTLDDLRGIDHNDTRLKRLEHVQSELQAVEQAIQEASAVHDAFNQRVREESKLFDSVRQSEMLSAISDYANVHVEFFTNIRDLWIRVKQ 1732E3D08B85157C yg06_schpo Uncharacterized protein C1604.06c SPBC1604.06c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIKDLENEIYKSRKNLNNILVLFDYIDLSNHSIDEVNDAAAALCRVYCYLSRNGLLKRPKEDDSSANAQVKNWVCDNYINYTEKLTEIFSMANVEALQVSFLTMTMRLCKAESQMDENGTFRNQFYIRFCLELLSSSQLSDICIKDFVTSYLVPYDDVRFFFYKNSKKVISSLIESSKTDDPMANLDIVAFNTIRILSAIPSPLPSSSTSSWADEPSPSSTETSSIKRAFQESWLSALSLPLSVNLYKQVLNVIHKRVIPFLQKPNLLMDFLTDAYNSHHAVSLLALNGLFTLMISHNLDYPLFYPKLYALLDRNLLYLKTRSRFFRLLDLFLSSTHLPATLIASFIKRLARLALTAPPGAIAIVIPFIYNCLQRHPTCMQMLHRSSAESGDSFDFDQPDPLLTGAIESSLWELSTLQNHYYSNIASLASIMSQKFTKPRYELEDFLDHGYATMCDAELRRPLKNEPPIEFEKRTLASGLEKSWI D65BADD2BE91C4AA skb1_schpo Protein arginine N-methyltransferase skb1 Type II protein arginine N-methyltransferase rmt5 SPBC16H5.11c Shk1 kinase-binding protein 1 skb1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLLRDGRIPQYSIYPLPVPPMNSKTPTLGIVCSEGTISLSLEEGFEFVGVPLSGEGLKLRVEALAPSERLQEFLDDEVAYHPEENVHKVVGLSSAWLELDSEDTLIADRSEEVLLKEASYASYCGLSSIILNGPTSPMNVMRYARAVSSALNSTMNLKFLVQLAIESGHEDYFETWKMWDTIRSACGYHPRLKVALELPPACSPPIELVNRWYAEPIEMITMSCMAFVPNPNGYPVLGRKLRAIYALYLRLNPRILLWDNDAPEKIGDSPDYSIYMKHLFDSQPPAPLVEDLADSYKDYLQVPLQPLSYNLENITYEIFERDPVKYAQYEQAIFSALMDRDESSVTRIAVVGAGRGPLVDCALRAAISSSRTVDMIALEKNPNAFSMLLMRNRQDWAGKVTLVFGDMRTWNPDYKIDILVSELLGSMGDNELSPECLDGVQHVLDEETGICIPSSYISYVTPIMSPKLWSEARNMNDPNAFERQYVVLMNSFDFLAADDEFRFQSLWSFHHPNKDSEVYTKNLHNKRFASVRFQASSPGILHGFAGYFEATLYKDISLSIMPATMEAKSPDMFSWFPIYMPIKKPMYVPENSQLEFHMWRLTDGMRVWFEWCANAYLVLRNGSQIKLSSTEVHNISGKAFSCNMY 288FEE7BDF61558D syf1_schpo Pre-mRNA-splicing factor cwf3 Complexed with cdc5 protein 3 cwf3 syf1 SPBC211.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGDVIPLKVNFDLINVDDEPFELELLRDPYSLKSWLRYIKTHEGSTLEKRVLLFERACSELPGSYKIWKSYLELRVAHVEHLNPYFHAEAFASVNDCFERSLILLHKMPVIWKLYLQFLMKQPNVTKIRCTFNSALRALPVTQHDDIWDMFTKYAEDIGGLFCIHVYRRYIQVEPRAIENYIEILCKLGLWNEAARQYEDILNRPVFLSAKRKSNYQIWLEFSELVVQHPDHTQNIDVEKVFRAGIKRFSDQAGKLWTYLAQYYIRIGDYEKARSTFYEGMNNIMTVRNFTIIFDAFVEFEEQWLSARVEASSGNANDELSIDFHMAWLEKILDKRPLYINDVLLRQNINNVDEWLRRVKFLEDDSEKVVQVYTDAIKNVNPKLAHGSLGKLFSEFARFYENFDDLEQSRIIFEKATHVPYKTVNELAQVWIDWAEMELRHQNFDAARKLIGDAVHAPRKSHISFFDESLSPQVRLHKSSKIWMYYLDLEESVGTIETTRKLYDRVFELKIATPQVVVNYANLLEENAYFEDSFKIYERGVALFSYPVAFELWNLYLTKFVKRYQGTHMERTRDLFEQALEGCPPEFSKSIYLLYADFEEKFGKAKRSISILEKAADKVKTADRLAIYNVLLVKVALNYGVLATRTVYEKAIESLSDSEVKDMCLRFAEMETKLGEIDRARLIYIHGSQYCDPRVETDYWKAWQEFEIRYGNPEETVKEMLRIKRSVQTKFSTDSLHIAKRAAKIESAAAPMDPMEQLEMEKSEGPKALAGFVLSKSNPQETSKITGEEN 4915D160DD83F324 hat2_schpo Histone acetyltransferase type B subunit 2 Kinetochore protein mis16 mis16 SPCC1672.10 hat2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSEEVVQDAPLENNELNAEIDLQKTIQEEYKLWKQNVPFLYDLVITHALEWPSLTIQWLPDKKTIPGTDYSIQRLILGTHTSGNDQNYLQIASVQLPNFDEDTTEFTPSTIRRAQATGSYTIEISQKIPHDGDVNRARYMPQKPEIIATMGEGGNAYIFDTTCHDALTTGEALPQAVLKGHTAEGFGLCWNPNLPGNLATGAEDQVICLWDVQTQSFTSSETKVISPIAKYHRHTDIVNDVQFHPQHEALLASVSDDCTLQIHDTRLNPEEEAPKVIQAHSKAINAVAINPFNDYLLATASADKTVALWDLRNPYQRLHTLEGHEDEVYGLEWSPHDEPILASSSTDRRVCIWDLEKIGEEQTPEDAEDGSPELLFMHGGHTNRISEFSWCPNERWVVGSLADDNILQIWSPSRVIWGRDHVQVSPRDLE 606A7D009E60EA20 tsc1_schpo Tuberous sclerosis 1 protein homolog tsc1 SPAC22F3.13 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPLQSLVKALWNVLHEEESEGYPDLTELIAEVESYQQRYPKQNPTNSQKIRHILDEIYEKTPFNNTRRRILWLAVLKTVIPLLILDRQAVGEWWDQIFFPFLNSPTQLKPVFSDLKSILFYILIFHDEDEWGGDLRRECAEETITRLVDLYVSKAIENLGDVESQEQRNQTIECLVNVLVHYGIQRPKELSSCFCHHFLNPPTRIPILSVMVEVIRRQGPRLYEIPQTGFYDLVLKCAEFDTSPILLSYALSFILMILSHICNSLDDSLYRLFCIYLRFSMIDPTSGFPSSTASGNWEVFHDFMSTYASTTTSQTDSSYNDVHDIVGSSQPDYLESLDYSQLFSILYALYPINFLEFLRDPKLYASKHNFQIRYSFNQELLSTKSDGLLGRHLAHSNFLKYTAETELTDKSRWTRLDSIAVVALCNSLNAVGIAESVMDPFGGKLPTTYEETSSATGLLAYPNESHDIASEPFSISWPQNPSISGSVHSATTFDKAQLSNTEDSYDNISHGTSYSEGVSSIHMVKGERGSNNLELTSESLSSTNDTIRRLQRDLLFLQNELRFEKFVRQQHLQNIGKLHREHILDMAVESERQKLLLTNKRYKAQIELLNSEIDKHRSESQAALNRRVKWENDFNNKIKALREEKKAWKSEESELKSSIESLISQLESIRNSQIDIAFSKNQLELKLQLYETKLKEYEQHLSCVNISKKQVSSSSDTSFGNTKMDSSMILSNSEAVSDEQERELIESEKHRMKLESENLHLQANIELLKKDLEAINVVYEAKIFDLEKRLSSEANAPELHNPVNLNYDAQLSKISEIKENYDELLTRYRELEGKFLESQAEVEELKNFQKPLVDTGSSIHSSPGLQQSKFIIRNDSLHPKVGPPRRQSTDTSRSTFRQY 03D7E9CC8866ECAB ima2_schpo Importin subunit alpha-2 Karyopherin subunit alpha-2 Importin-1 imp1 SPBC1604.08c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MESRYLSDRRSRFKSKGVFKADELRRQREEQQIEIRKQKREESLNKRRNLNAVLQNDIDVEEEADQSQVQMEQQMKDEFPKLTADVMSDDIELQLGAVTKFRKYLSKETHPPIDQVIACGVVDRFVQFLESEHHLLQFEAAWALTNIASGTTDQTRIVVDSGAVPRFIQLLSSPEKDVREQVVWALGNIAGDSSACRDYVLGNGVLQPLLNILQSSASDVSMLRNATWTLSNLCRGKNPPPNWSTISVAVPILAKLLYSEDVEIIVDACWAISYLSDGPNEKIGAILDVGCAPRLVELLSSPSVNIQTPALRSVGNIVTGTDAQTQIIIDCGALNAFPSLLSHQKENIRKEACWTISNITAGNTQQIQAIIESNLIPPLVHLLSYADYKTKKEACWAISNATSGGLGQPDQIRYLVSQGVIKPLCDMLNGSDNKIIQVALDAIENILKVGEMDRTMDLENINQYAVYVEEAGGMDMIHDLQSSGNNDIYLKAYSIIEKYFSDEDAVEDLAPETENGAFTFGNGPQAQGEFKFDSQDMAM FFD4919E936E0941 ess2_schpo Stress response protein bis1 bis1 Splicing factor ESS-2 homolog SPCC364.02c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLAKKIDDDEKQLVKPVNKEQTYKKPIPLEEDDYIEGLSYIIQQQYFPDLPKLKAEVVLESEEVGSFDAQNESRDEKLKYLIAKNSEDPLRKRLPSLAIHEITKAQLDGENKPISVASYQNKFTSEDNASFGELMEDESRLRAEQHKRRFGVHSQQPSNSIQTIGYSNSDAIKSIAWKEKDKSIKTWNYQPKNALMYTPETNHSSSLSQIKKQSTEIQADATGLSQSFLEAANQPSTDPIVPPSVNETDASVNGYPLVDVNFGQAVGSSSKSYFNIPERPRRERLHAMRVRDIRSHSTNTTITSVDSASTALNSYSTPNSVSRKLTNLTPAARRLVARSYLRSPLHGSSPSASRHTALRTSIPKFSWTPTPRVKSTAPTPKRV 0DC9D851B655C695 mcm6_schpo DNA replication licensing factor mcm6 mcm6 mis5 SPBC211.04c Minichromosome maintenance protein 6 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSLASQGNNASTPAYRYGFQTSEVGDRPTVSMPSQPESSAMLEDDGMVKRKPFAAIGEAIPKVIDTTGESVREAFEEFLLSFSDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDYKHLTSYNDVLALAIVEQYYRFSPFLLRALQKLIEKFEPEYYRSSLSRENASLSPNFKASDKTFALAFYNLPFRSTIRDLRTDRIGRLTTITGTVTRTSEVRPELAQGTFICEECHTVVSNVEQAFRYTEPTQCPNELCANKRSWRLNISQSSFQDWQKVRIQENSNEIPTGSMPRTLDVILRGDIVERAKAGDKCAFTGILIAVPDVSQLGIPGVKPEAYRDSRNFGGRDADGVTGLKSLGVRDLTYKLSFLACMVQPDDANDKSGADVRGDGSQGIEEQDEFLQSLSQEEIDDLRAMVHSDHIYSRLTNSLAPSVYGHEIIKKGILLQLMGGVHKLTPEGINLRGDLNICIVGDPSTSKSQFLKYVCNFLPRAIYTSGKASSAAGLTAAVVKDEETGDFTIEAGALMLADNGICAIDEFDKMDLSDQVAIHEAMEQQTISIAKAGIQATLNARTSILAAANPIGGRYNRKTTLRNNINMSAPIMSRFDLFFVVLDECNESVDRHLAKHIVDIHRLRDDAMQPEFSTEQLQRYIRYARTFKPKLNTESCAEIVKKYKQLRMDDAQGAGKNSYRITVRQLESMIRLSEAIARANCVDDITPAFVNEAYSLLRQSIIHVERDDIEVEEDDAEAQELENDNTNTTNGNDNVSSEEALQKPKVKITYDKYVSIMNGILQVLRQRSTEGVDGVPAGDLVQSYLELREDQFHTEEDIIYEVGLVRKVLTRLVHESIIMEIQNLTDSAVRLPFEERVFSIHPNCDIDALLSNGDVPN 3A00DB55B2EE08B7 pli1_schpo E3 SUMO-protein ligase pli1 pli1 E3 SUMO-protein transferase plil SPAC1687.05 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNQANFLQELPNVLKRLETGLIIPQLKDILRVFGLRLSGTKAELITRIKQLIERIAIENNTTSWEALKKAIDGDVTSAVCILKYNTYQIYSAAAPIAPPSSASGNRSYSRPFAPVVHSRIRFRKSPFYDILEQFNAPFVVPACVGTRNTISFSFHVTPPALSKLLNDPKQYRVYLFSTPSETIGFGNCLMEFPTPQMELRINNQVAHANYRRLKGKPGTTNPADITDLVSKYAGPPGNNVVIYYMNSTKSYSVVVCFVKVYTIENLVDQIKSRKAESKEKIIERIKNDNQDADIIATSTDISLKCPLSFSRISLPVRSVFCKHIQCFDASAFLEMNKQTPSWMCPVCASHIQFSDLIIDGFMQHILESTPSNSETITVDPEGNWKLNTFDEPVESSEDEFVPKEKVIELSDGEGISTMANKSNDQPTRRASTHNSGPPAKRKRESLVIDLTISDDDENVATSTTESPSNATKENSLSRNVQSPNIDTAISNRSTNVRHGHPGFKDYTVENSPASRERSTSESAQSSVHMGYAGEGGLLSGALRAPSQQNNNNSNTQHSINLHTIVPSPYEPPLSVTPSTAITNLSIPESNRTNSSASSKSFTMNDLILPPLHLKNTTQTNNAHEDAQSSNLSQNHSLFYERIPQRPSYRIEKQNKGIYEDENEQSISAMPIPRAHPQLPKNLLSQTAGPLWDEQQDAQVDWNSELQSNNSYHNSGFEGTGNTFQSID 56228A04888DD235 56228A04888DD235 lsm3_schpo Probable U6 snRNA-associated Sm-like protein LSm3 lsm3 SPBC9B6.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MESAQAVAEPLDLVRLSLDEIVYVKLRGDRELNGRLHAYDEHLNMVLGDAEEIVTIFDDEETDKDKALKTIRKHYEMLFVRGDSVILIAPPRN 86874A78670B1DCE us107_schpo U1 snRNP-associated protein usp107 usp107 snu71 SPBC839.10 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQRQNTQAAGMPMMPQVPMVGNGVPYVVPIQPVFAPLPPDYRSLYKKLYGQGAFLVDNPVEASSPYDFSQPILKFGKLPIKQVLRDNESQQKDRKNLPRNQKSNEIQEKQTFQTPSSEKSTTERESRPFVPPNSQQMRRMLFIGNIPKELDDFWMDKILRLSGKLASWRRVADADNSMTSFGFAEFESNEQFSRALEALNDFVVPPLYEGGPSTRLSLITDVENEGLYREWQTSRYARNKQKEINILQQIRFNLERICQDIGNFDVRSRIERAARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQSAMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISRIPMSTVNAFLRAEDSIPESFSDEQAYYEEKRRKDQLEAEEYYARERRWMNREKARTAALEREAAREEEERVNNTSFGTYLSEKLASFDDDEEARVSRDEYFVDRAAWIRHRAVARAREEDADALDRKEEERELRTRGEGATVETENYVENGKLVTSEMPQHENGPFKIKIQTKKPAVPSERREFGLPERLLLEEEDEEPQGYSPNPQKPKPAMEENDAEKTKRLRSLIEKIPVEAESLWALPIDWSKVTEDLLKEEMQAFVTKKIIEYIGIQEDSLITFTIDHIRQHKGAEQLVSELDLALAEDAPEFVSKVYRYLHVLLILRSEA 5B56816D3CEE47BA ish1_schpo Stress response protein ish1 ish1 isp1 SPBC365.12c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MRSRSVASLGVLLAIVFYIFTYGFSTYRKNDTSAVKTWLEEHSIPYGSRSSPSDFQQFISESYDSLVPNLDKWSGHNLNSWLGKKTESSYLDAISNKIRKTGRKLSGSVEDARDATQEAWESQKASLFESWSDSQLRAFLARHSPQFAAKEKKGILEKLSPASLRETAAKEYDALTSKLGNTGDWIYDTWSDNELRTWLHDVGVPISSHESTRSHLLRKLKNYISTKADEAQPSVEAVKGKASEKAKQAGEFVSDKAGDAKELVNEKSSEAGQYAGQKMEEGGELLQEISKRRGRFGNWWANSGLKAYFDAHGIPAYQPSPIDQFYAHLRRQYYLKTNGYQALKSKAYEEASNVADSASSIASNVASGATEAYQGAASGASRFTEGAKTAAESVTSAFEHNKDTATSKAKQMKGKAASLGSTASEKVGEAADYAAETASKVASKAASKVRETIDETIIERWQDSKLKEFLFLRGVPVPQRSTKDQLLDLVRKHFNKGAVPNWAAYFDTLSSKELSAWIKEYKKHYHGKLHPSKDREHLFQNACNIYRAIAEKADKSVLQSIQSKFPKATVPGYDSWSNEDLKSALKEYGDSIGKVFNRKDAIERLKRHDILFYGPVVADKAKSGVSGFLRRVASFMGFGTRDVAEIKLGENIQKVKDTASKASSAVSSAGDYVETNVKKAQRVL 884CE69714BC7FAB gblp_schpo Guanine nucleotide-binding protein subunit beta-like protein Receptor of activated protein kinase C rkp1 cpc2 SPAC6B12.15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPEQLVLRATLEGHSGWVTSLSTAPENPDILLSGSRDKSIILWNLVRDDVNYGVAQRRLTGHSHFVSDCALSFDSHYALSASWDKTIRLWDLEKGECTHQFVGHTSDVLSVSISPDNRQVVSGSRDKTIKIWNIIGNCKYTITDGGHSDWVSCVRFSPNPDNLTFVSAGWDKAVKVWDLETFSLRTSHYGHTGYVSAVTISPDGSLCASGGRDGTLMLWDLNESTHLYSLEAKANINALVFSPNRYWLCAATGSSIRIFDLETQEKVDELTVDFVGVGKKSSEPECISLTWSPDGQTLFSGWTDNLIRVWQVTK 4E14707164E68ACD rrp41_schpo Exosome complex component ski6 Ribosomal RNA-processing protein 41 SPAC3G9.10c ski6 rrp41 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSHFEILSLEGLRNDGRRWDEMRNFQCRIGIEPSENGSAFIELGNTKVLCIVDGPSEPVIKSKARADRTFVNVEINIASFSTIDVKKRFKSDRRIQLQCLALQNTFEEIIQTELYPRSQISVYLHVLQDDGAVMASCINATTLALIDAGIPVKDFVCCSTAGIVESDMLLDLNSLEESALSWLTVAVLGNIKKVVYMQLETSMHLDYLESVMNMAIAGSEHIYNTMQSAVRQSAKPALASLS 0EE46F5F994584A9 bis1-ish1-1 4 22 unspecified role unspecified role bait bait c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 27 unspecified role unspecified role prey prey c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 5C, 5D Fig 5A specifies amino acids 34-580 of ish1, while materials and methods specifies amino acids 1-580. ish1-ish1-1 3 27 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 27 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 40 38 physical association physical association Table III The Ish1-Ish1 interaction is largely unaffected by the N-terminal deletion. ish1-bis1-1 3 22 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 27 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 43 45 physical association physical association Table III ish1-rps6a-1 3 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 27 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 50 48 physical association physical association Table III ish1-bis1-2n 2 22 unspecified role unspecified role neutral component neutral component c-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 27 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig 6D, 6E When Bis1-YFP and Ish1-GFP are co-overexpressed in wild type cells, they display partial overlap in their localization at the nuclear envelope (Fig. 6, D and E). bis1-snx4-1 1 15 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 60 58 physical association physical association Table IV bis1-pct1-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 63 65 physical association physical association Table IV bis1-rkp1-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 28 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 70 68 physical association physical association Table IV bis1-mcm6-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 73 75 physical association physical association Table IV bis1-skb1-1 1 17 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table IV bis1-rpn6-1 1 14 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table IV bis1-rrp41-1 1 29 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 90 88 physical association physical association Table IV bis1-abp1-1 1 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table IV bis1-lcm3-1 1 25 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table IV bis1-nup211-1 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 105 103 physical association physical association Table IV bis1-imp1-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 21 unspecified role unspecified role prey prey physical association physical association Table IV bis1-cpy1 -1 1 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 114 112 physical association physical association Table IV bis1-kap123 -1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 117 119 physical association physical association Table IV bis1-nuf2-1 1 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table IV bis1-pli1-1 1 24 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 129 127 physical association physical association Table IV bis1-spbc1604_06c-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 132 134 physical association physical association Table IV bis1-spbc839_10-1 1 26 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table IV bis1-reb1-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey physical association physical association Table IV bis1-tsc1-1 1 20 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 148 146 physical association physical association Table IV bis1-hat2-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 151 153 physical association physical association Table IV bis1-cwf3-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 18 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table IV bis1-rlf2-1 1 22 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 163 161 physical association physical association Table IV ish1-bis1-2 1 22 unspecified role unspecified role prey prey 27 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table III ish1-ish1-2 1 27 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 27 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 172 170 physical association physical association Table III The Ish1-Ish1 interaction is largely unaffected by the N-terminal deletion. ish1-rps6a-2 1 27 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey physical association physical association Table III