UniProt UniProt http://www.uniprot.org Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling. Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling. Dev. Biol. (0012-1606) 2001 imex curation Wharton KA., Zimmermann G., Rousset R., Scott MP. keith.wharton@utsouthwestern.edu 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wharton KA., Zimmermann G., Rousset R., Scott MP. keith.wharton@utsouthwestern.edu keith.wharton@utsouthwestern.edu Accepted 2007-NOV-15 by ORCHARD. Dev. Biol. (0012-1606) 2001 orchard Only protein-protein interactions imex curation Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling. Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling. Dev. Biol. (0012-1606) 2001 imex curation Wharton KA., Zimmermann G., Rousset R., Scott MP. keith.wharton@utsouthwestern.edu 13-Nov-2007: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Wharton KA., Zimmermann G., Rousset R., Scott MP. keith.wharton@utsouthwestern.edu keith.wharton@utsouthwestern.edu Accepted 2007-NOV-15 by ORCHARD. 13-Nov-2007: Contacted by JYOTI. Dev. Biol. (0012-1606) 2001 orchard Only protein-protein interactions imex curation dvl2_mouse Segment polarity protein dishevelled homolog DVL-2 Dvl2 DSH homolog 2 protein protein mouse Mus musculus Mouse MAGSSAGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDTPQPEVAPPAHESRTELVPPPPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRDRPRRRDSSEHGAGGHRPGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRMERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGAFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHMDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESELSSRGGSLRRGGEPGGTGDGGPPPSRGSTGAPPNLRALPGLHPYGAPSGMALPYNPMMVVMMPPPPPPVSTAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein Q60838 original sequence version: 132 DIP protein Q60838 original sequence version: 63 DIP protein Q60838 original sequence version: 125 DIP protein Q60838 original sequence version: 69 DIP protein Q60838 original sequence version: 91 928A527143BBBBB0 nkd_drome Protein naked cuticle nkd CG11614 dNkd protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAGNIVKWWKHKILGGYKQFSVQECTTDSEELMYHQVRASSSCSAPPDLLLVSERDNNIQLRSPVVNIITTPPGNASGAGSKQQSHHQTNHHSSGRSHPGHTAHPQDVSSGGSHSKHLRISSTSNGKHGKYSNMQQQLPQDEDVVDAAATMQQQQHTGHAHSRHLHHHKEERIRLEEFTCDVSVEGGKSSQPLQFSFTFYDLDGHHGKITKDDIVGIVYTIYESIGKSVVVPHCGSKTINVRLTVSPEGKSKSQPVVPVPVAAGFSSSHASKLKKLPTGLAAMSKPLAGGGVGSGGASALTTSAGNRRQHRYRPRKLIKSDDEDDDSNSEKEKDAAHAPAADQPSGSGTKATGKSHHHQSQSARYHQKNNSRAEQCCTEQNTPDNGHNTYENMLNLKCCKPEVDQVDCPSHRQHHQSHPNHQMRQQDIYMKQATQRVKMLRRARKQKYQDHCLETRQRSLSVGNDSACPNRHLQLQQPPVGHPQPQSLNHKSASGSPPLGVGGGGDMMLDGVQLRQPRPHSLTPHQHQQQNQQQQQQQRKSAECWKSALNRNDLISIIRESMEKNRLCFQLNGKPQANVSPIRQPAAQQQPQQQQRQRCNTGSKIPTLITNHSPVAQQSPLSCSPPTAEPTTPSIPAAPPAIEVNGQQHHPTHPTHPSHHNHHEHPQPHIPIYHQQLAINPAVLAAQQTHNTAHNKLNLCGYDSFLHATICGGGAAAHSPPATPSNVATVQPIPKKSQKNLLQGYQRLEQSQQQQQQQRSSKDYKNYGNLIYAKLSEQLQQKDREQRRQRHKQQQHQMLQDQPKDASRSEQRPPTSNSSSAGSKIYGDAVECAHLLASEEEDLPPSPQLTSTPSKVVSTDTLIDLNDDVGEAVAEAVTEGGKQSLEAEESGQQVEVELDTSASSSMIHRYVHEHIHHHYHHFKEQQDV 4510732C7A77AA5F dvl3_mouse Segment polarity protein dishevelled homolog DVL-3 Dvl3 DSH homolog 3 protein protein mouse Mus musculus Mouse MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPEPAPFCADNPSELPPSMERTGGIGDSRPPSFHPHASGGSQENLDNDTETDSLVSAQRERPRRRDGPEHAARLNGTTKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLTSSLRSHHTHPSYGPPGVPPLYGPPMLMMTPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMGNPSEFFVDVM 552A5BE4BEA801F0 dsh_drome Segment polarity protein dishevelled dsh CG18361 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDADRGGGQETKVIYHIDDETTPYLVKIPIPSAQVTLRDFKLVLNKQNNNYKYFFKSMDADFGVVKEEIADDSTILPCFNGRVVSWLVSADGTNQSDNCSELPTSECELGMGLTNRKLQQQQQQHQQQQQQQQQQHQQQQQQQQQQVQPVQLAQQQQQQVLHHQKMMGNPLLQPPPLTYQSASVLSSDLDSTSLFGTESELTLDRDMTDYSSVQRLQVRKKPQRRKKRAPSMSRTSSYSSITDSTMSLNIITVSINMEAVNFLGISIVGQSNRGGDGGIYVGSIMKGGAVALDGRIEPGDMILQVNDVNFENMTNDEAVRVLREVVQKPGPIKLVVAKCWDPNPKGYFTIPRTEPVRPIDPGAWVAHTQALTSHDSIIADIAEPIKERLDQNNLEEIVKAMTKPDSGLEIRDRMWLKITIPNAFIGADAVNWVLENVEDVQDRREARRIVSAMLRSNYIKHTVNKLTFSEQCYYVVNEERNPNLLGRGHLHPHQLPHGHGGHALSHADTESITSDIGPLPNPPIYMPYSATYNPSHGYQPIQYGIAERHISSGSSSSDVLTSKDISASQSDITSVIHQANQLTIAAHGSNKSSGSSNRGGGGGGGGGGNNTNDQDVSVFNYVL 0BA24ED350666CF5 dvl1_mouse Segment polarity protein dishevelled homolog DVL-1 Dvl1 Dvl DSH homolog 1 protein protein mouse Mus musculus Mouse MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESMVSHRRERARRRNRDEAARTNGHPRGDRRRDLGLPPDSASTVLSSELESSSFIDSDEEDNTSRLSSSTEQSTSSRLVRKHKCRRRKQRLRQTDRASSFSSITDSTMSLNIITVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTIPRADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAITRTSSSSLTSSVPGAPQLEEAPLTVKSDMSAIVRVMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSMLKHGFLRHTVNKITFSEQCYYVFGDLCSNLASLNLNSGSSGASDQDTLAPLPHPSVPWPLGQGYPYQYPGPPPCFPPAYQDPGFSCGSGSAGSQQSEGSKSSGSTRSSHRTPGREERRATGAGGSGSESDHTVPSGSGSTGWWERPVSQLSRGSSPRSQASAVAPGLPPLHPLTKAYAVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM DIP protein P51141 original sequence version: 147 FA21ACAC48FF71E0 nkd1_mouse Protein naked cuticle homolog 1 Nkd1 protein protein mouse Mus musculus Mouse MGKLHSKPAAVCKRRESPEGDSFAVSAAWARKGIEEWIGRQRCPGSVSGPRQLRLAGTVGRGTRELVGDTSREALGEEDEDDFPLEVALPPEKIDSLGSGDEKRMERLSEPGQASKKQLKFEELQCDVSVEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKTLRVKLTVAPDGSQSKRSVLFNHTDLQSTRPRADTKPAEELRGWEKKQRAPLRFQGDSHLEQPDCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVAQKSELPPRISNPTRSRSHEPEAAHIPHRRPQGVDPGSFHLLDTPFAKASELQQRLRGTQDGSKHFVRSPKAQGKNMGMGHGARGARSKPPLVPTTHTVSPSAHLATSPALLPTLAPLGHKKHKHRAKESQASCRGLQGPLAAGGSTVMGREQVRELPAVVVYESQAGQAVQRHEHHHHHEHHHHYHHFYQP 3E85E77B382F5C3B nkd2_mouse Protein naked cuticle homolog 2 Nkd2 protein protein mouse Mus musculus Mouse MGKFQSKHAAAACKRRESPEGDSFVVPAYGSGRRGAEETDRRAGSGVEHRSRDKQELLNGDPKEGPFWDDKGSLEVVLPPEKSEGHEGQGQLFSTDDGEKAASREGPLRLSKKHLNIDALQCDVSVEEDNRQEWTFTLYDFDNSGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKKECPLTGQDREPTRGRTEIELTDEPRVADRRLSAYSRKPNADPQPCSVRVPYCVDENTERRNHYLDLAGIENYTSKFGPGSPPEQARQEHHGRATHIPSRSRSQESDAHAIHHRRSQVLAEHVIPANEPATRALAAQPRIKGQEKQFLRSPKGPGKPLGTPGSGKPGKALSYCLQAVPLPQSAQDGHHLPQPPPQPPPQPYGHKRYRQKAREGHSPLKGHGQPTMVEHEVVRDLPPMLGPEGYVMPVVQRHEHHHHHEHHHHHHHHHFHPS 7C3B550024E87D27 nkd1-dvl1-1 1 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd2-dvl1-1 1 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd-dvl1-1 1 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A dsh-nkd-3 2 6 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig 3B In vitro translated mNkd1, similar to fly Nkd, specifically bound glutathione sepharose beads to which a GST-Dsh fusion protein was attached (Fig. 3B). nkd1-dvl2-1 1 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd1-dvl3-1 1 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd1-dsh-2 1 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd-dvl2-1 1 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd-dvl3-1 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd-dsh-6 1 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd2-dvl2-1 1 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd2-dvl3-1 1 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A nkd2-dsh-1 1 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3A dsh-nkd1-1 2 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 6 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 3B In vitro translated mNkd1, similar to fly Nkd, specifically bound glutathione sepharose beads to which a GST-Dsh fusion protein was attached (Fig. 3B).