IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ FTZ-Factor1 and Fushi tarazu interact via conserved nuclear receptor and coactivator motifs. FTZ-Factor1 and Fushi tarazu interact via conserved nuclear receptor and coactivator motifs. EMBO J. (0261-4189) 2001 imex curation Schwartz CJ., Sampson HM., Hlousek D., Percival-Smith A., Copeland JW., Simmonds AJ., Krause HM. h.krause@utoronto.ca 12-Oct-2007: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag visualisation tag visualisation Schwartz CJ., Sampson HM., Hlousek D., Percival-Smith A., Copeland JW., Simmonds AJ., Krause HM. h.krause@utoronto.ca Accepted 2007-OCT-27 by ORCHARD. EMBO J. (0261-4189) 2001 orchard Only protein-protein interactions imex curation ftzf1_drome Nuclear hormone receptor FTZ-F1 ftz-f1 NR5A3 CG4059 FTZ-F1 alpha Nuclear receptor subfamily 5 group A member 3 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDTFNVPMLAESSNTNYATEATSNHHHLQHQHQQQHSHQQQQQQQLLMPHHHKDQMLAAGSSPMLPFYSHLQLQQKDATATIGPAAAAAAVEAATTSANADNFSSLQTIDASQLDGGISLSGLCDRFFVASPNPHSNSNMTLMGTATAATTTTTNNNNNNNTNNNNNNNVEAKTVRPSNGNSVIIESVTMPSFANILFPTHRSANECIDPALLQKNPQNPNGNNSSIIVPPVEYHQLKPLEVNSSTSVSTSNFLSSTTAQLLDFEVQVGKDDGHISTTTTTGPGSGSASGSGSGSGSGSGSIARTIGTATPTTTTSMSNTANPTRSSLHSIEELAASSCAPRAASPNSNHTSSASTTPQQQQQQQHHMQSGNHSGSNLSSDDESMSEDEFGLEIDDNGGYQDTTSSHSQQSGGGGGGGGGNLLNGSSGGSSAGGGYMLLPQAASSSGNNGNPNAGHMSSGSVGNGSGGAGNGGAGGNSGPGNPMGGTSATPGHGGEVIDFKHLFEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNKKVYTCVAERSCHIDKTQRKRCPYCRFQKCLEVGMKLEAVRADRMRGGRNKFGPMYKRDRARKLQVMRQRQLALQALRNSMGPDIKPTPISPGYQQAYPNMNIKQEIQIPQVSSLTQSPDSSPSPIAIALGQVNASTGGVIATPMNAGTGGSGGGGLNGPSSVGNGNSSNGSSNGNNNSSTGNGTSGGGGGNNAGGGGGGTNSNDGLHRNGGNGNSSCHEAGIGSLQNTADSKLCFDSGTHPSSTADALIEPLRVSPMIREFVQSIDDREWQTQLFALLQKQTYNQVEVDLFELMCKVLDQNLFSQVDWARNTVFFKDLKVDDQMKLLQHSWSDMLVLDHLHHRIHNGLPDETQLNNGQVFNLMSLGLLGVPQLGDYFNELQNKLQDLKFDMGDYVCMKFLILLNPSVRGIVNRKTVSEGHDNVQAALLDYTLTCYPSVNDKFRGLVNILPEIHAMAVRGEDHLYTKHCAGSAPTQTLLMEMLHAKRKG 6A2240934830290D ftz_drome Segmentation protein fushi tarazu ftz CG2047 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MATTNSQSHYSYADNMNMYNMYHPHSLPPTYYDNSGSNAYYQNTSNYQGYYPQESYSESCYYYNNQEQVTTQTVPPVQPTTPPPKATKRKAEDDAASIIAAVEERPSTLRALLTNPVKKLKYTPDYFYTTVEQVKKAPAVSTKVTASPAPSYDQEYVTVPTPSASEDVDYLDVYSPQSQTQKLKNGDFATPPPTTPTSLPPLEGISTPPQSPGEKSSSAVSQEINHRIVTAPNGAGDFNWSHIEETLASDCKDSKRTRQTYTRYQTLELEKEFHFNRYITRRRRIDIANALSLSERQIKIWFQNRRMKSKKDRTLDSSPEHCGAGYTAMLPPLEATSTATTGAPSVPVPMYHHHQTTAAYPAYSHSHSHGYGLLNDYPQQQTHQQYDAYPQQYQHQCSYQQHPQDLYHLS 517154F3DAF8262F ftz-ftz_f1-1 1 2 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 1, 3 Ftz_drome constructs with deletions of 112-118 and 257-265 show decreased interaction suggesting that they are both involved in the interaction. a far western blot containing several FTZ deletion constructs probed with 35S-labeled FTZ-F1. The full-length FTZ polypeptide is recognized well by the radiolabeled FTZ-F1 probe ftz-ftz_f1-2 1 3 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind range range range range direct interaction direct interaction 5 The C-terminal half of FTZ-F1 (A+LBD) binds well to the FTZ probe (Figure 5A, lane 1), as previously shown in Figure 3B.