IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis NPH3: A NPH1 photoreceptor-interacting protein essential for phototropism. Arabidopsis NPH3: A NPH1 photoreceptor-interacting protein essential for phototropism. Science (0036-8075) 1999 imex curation Motchoulski A., Liscum E. liscumm@missouri.edu 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Motchoulski A., Liscum E. liscumm@missouri.edu Accepted 2007-DEC-19 by JYOTI. Science (0036-8075) 1999 Only protein-protein interactions imex curation Arabidopsis NPH3: A NPH1 photoreceptor-interacting protein essential for phototropism. Arabidopsis NPH3: A NPH1 photoreceptor-interacting protein essential for phototropism. Science (0036-8075) 1999 imex curation Motchoulski A., Liscum E. liscumm@missouri.edu 22-Jan-2008: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Motchoulski A., Liscum E. liscumm@missouri.edu Accepted 2007-DEC-19 by JYOTI. 22-Jan-2008: Contacted by JYOTI. Science (0036-8075) 1999 Only protein-protein interactions imex curation phot1_arath Phototropin-1 Non-phototropic hypocotyl protein 1 Root phototropism protein 1 PHOT1 JK224 NPH1 RPT1 T6D9_110 At3g45780 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEPTEKPSTKPSSRTLPRDTRGSLEVFNPSTQLTRPDNPVFRPEPPAWQNLSDPRGTSPQPRPQQEPAPSNPVRSDQEIAVTTSWMALKDPSPETISKKTITAEKPQKSAVAAEQRAAEWGLVLKTDTKTGKPQGVGVRNSGGTENDPNGKKTTSQRNSQNSCRSSGEMSDGDVPGGRSGIPRVSEDLKDALSTFQQTFVVSDATKPDYPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSGTDADELAKIRETLAAGNNYCGRILNYKKDGTSFWNLLTIAPIKDESGKVLKFIGMQVEVSKHTEGAKEKALRPNGLPESLIRYDARQKDMATNSVTELVEAVKRPRALSESTNLHPFMTKSESDELPKKPARRMSENVVPSGRRNSGGGRRNSMQRINEIPEKKSRKSSLSFMGIKKKSESLDESIDDGFIEYGEEDDEISDRDERPESVDDKVRQKEMRKGIDLATTLERIEKNFVITDPRLPDNPIIFASDSFLELTEYSREEILGRNCRFLQGPETDLTTVKKIRNAIDNQTEVTVQLINYTKSGKKFWNIFHLQPMRDQKGEVQYFIGVQLDGSKHVEPVRNVIEETAVKEGEDLVKKTAVNIDEAVRELPDANMTPEDLWANHSKVVHCKPHRKDSPPWIAIQKVLESGEPIGLKHFKPVKPLGSGDTGSVHLVELVGTDQLFAMKAMDKAVMLNRNKVHRARAEREILDLLDHPFLPALYASFQTKTHICLITDYYPGGELFMLLDRQPRKVLKEDAVRFYAAQVVVALEYLHCQGIIYRDLKPENVLIQGNGDISLSDFDLSCLTSCKPQLLIPSIDEKKKKKQQKSQQTPIFMAEPMRASNSFVGTEEYIAPEIISGAGHTSAVDWWALGILMYEMLYGYTPFRGKTRQKTFTNVLQKDLKFPASIPASLQVKQLIFRLLQRDPKKRLGCFEGANEVKQHSFFKGINWALIRCTNPPELETPIFSGEAENGEKVVDPELEDLQTNVF DIP protein O48963 original sequence version: 137 C9B68812F1B3A04E rpt3_arath Root phototropism protein 3 RPT3 JK218 NPH3 BTB/POZ domain-containing protein RPT3 Non-phototropic hypocotyl protein 3 MSJ1.17 At5g64330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMWESESDGGVGVGGGGGREYGDGVLSSNKHGGVKTDGFELRGQSWFVATDIPSDLLVKIGDMNFHLHKYPLLSRSGKMNRLIYESRDPDPTILILDDLPGGPEAFELASKFCYGVPVDLTATNISGLRCAAEYLEMTEDLEEGNLIFKTEAFLSYVVLSSWRDSILVLKSCEKLSPWAENLQIVRRCSESIAWKACSNPKGIRWAYTGKAPSPSTTNFAGSSPRWNESKDSSFYCSPSRNTNSQPVPPDWWFEDVSILRIDHFVRVITAIKVKGMRFELLGAVIMHYAGKWLPGLIKEGGVAIAPAMSSAIGGGLGLGGDEMSISCGSNSSGGSSGPDWKGGLHMVLSAGKTNGHQDSVACLAGLGISPKDQRMIVESLISIIPPQKDSVTCSFLLRLLRAANMLKVAPALITELEKRVGMQFEQATLQDLLIPGYNNKGETMYDVDLVQRLLEHFLVQEQTEGSSPSRMSPSPSQSMYADIPRGNNNNGGGGGGNNQNAKMRVARLVDSYLTEVARDRNLPLTKFQVLAEALPESARTCDDGLYRAIDSYLKAHPTLSEHERKRLCRVMDCQKLSMDACMHAAQNERLPLRVVVQVLFSEQVKISNALANTSLKESTTLGEAMGTYQPMIPNRKTLIEATPQSFQEGWAAAKKDINTLKFELETVKTKYVELQNEMEVMQRQFEKTGKVKNTPSSSAWTSGWKKLSKLTKMSGQESHDISSGGEQAGVDHPPPRKPRRWRNSIS DIP protein Q9FMF5 original sequence version: 93 7D91D4224A5F3549 nph3-nph1 1 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1553872]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 583-583, sequence length 558 [EBI-1553872]469-583 physical association physical association 4b significant interactions were only observed between coiled-coil-containing NPH3 polypeptide fragments and the NH2-terminal two-thirds of NPH1 nph3-nph1-1 2 4 unspecified role unspecified role bait bait region cellulose binding domain CBD tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1553889]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 745-745, sequence length 558 [EBI-1553889]469-745 ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association 4c The NPH3C2-CBD�dependent immunoprecipitation of NPH1 was not the result of nonspecific interaction with CBD, as a BTB/ POZ-containing NPH3-CBD fusion protein did not promote a similar immunoprecipitation response