IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Rapid divergence in the course of Drosophila evolution reveals structural important domains of the Notch antagonist Hairless. Rapid divergence in the course of Drosophila evolution reveals structural important domains of the Notch antagonist Hairless. Dev. Genes Evol. (0949-944X) 1999 imex curation Marquart J., Alexief-Damianof C., Preiss A., Maier D. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Marquart J., Alexief-Damianof C., Preiss A., Maier D. Accepted 2008-FEB-9 by DAVET. Dev. Genes Evol. (0949-944X) 1999 davet Only protein-protein interactions imex curation suh_drome Suppressor of hairless protein Su(H) dRBP-JK J kappa-recombination signal-binding protein RBP-J kappa CG3497 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKSYSQFNLNAAAPPAIAYETTVVNPNGSPLDPHQQQQQQSQDMPHFGLPGPQPPSSQQQQQQLQVHHQQQQQQQQQQQQQQHQQQMQMSLLPGPYRPHIEEKKLTRDAMEKYMRERNDMVIVILHAKVAQKSYGNEKRFFCPPPCIYLFGSGWRRRYEEMLQQGEGEQGAQLCAFIGIGSSDQDMQQLDLNGKQYCAAKTLFISDSDKRKHFMLSVKMFYGNGHDIGVFNSKRIKVISKPSKKKQSLKNADLCIASGTNVALFNRLRSQTVSTRYLHVENGHFHASSTQWGAFTIHLLDDNESESEEFQVRDGYIHYGATVKLVCSVTGMALPRLIIRKVDKQMALLEADDPVSQLHKCAFYMKDTDRMYLCLSQEKIIQFQATPCPKEPNKEMINDGACWTIISTDKAEYQFYEGMGPVASPVTPVPIVNSLNLNGGGDVAMLELSGDNFTPHLQVWFGDVEAETMYRCTETLLCVVPEISQFRGEWLWVRQPTQVPISLVRNDGIIYATGLTFTYTPEPGPRPHCNTQAEDVMRARQNNNNNNITSISNNNNSNNAGSPAAGGGLQQQQQQHQALPSISEVQWNSHGSGLS DIP protein P28159 original sequence version: 142 145144F336CD8F4C hles_drome Protein hairless H CG5460 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALLNDVTSVAECNRQTTMTDEHKSNINSNSSHSSNNNNNGSSSNNDNNSNDDAASSSNSKNNNTSNESSHSNNNTSSIIAEAAAKFLLKNGLNGSSSTSYPPLPPPLPANLSRTTTPTTTTTPSSSSSTASNGFLPHAKTPKSSSIMAASAAVAASVVGATASKPTIDVLGGVLDYSSLGGAATGSLPTTAVVAAAAGTAKIGKGSNSGGSFDMGRTPISTHGNNSWGGYGGRLQFFKDGKFILELARSKDGDKSGWVSVTRKTFRPPSAATSATVTPTSAVTTAYPKNENSTSLSFSDDNSSIQSSPWQRDQPWKQSRPRRGISKELSLFFHRPRNSTLGRAALRTAARKRRRPHEPLTTSEDQQPIFATAIKAENGDDTLKAEAAEAVEIENVAVADTTTNEIKIEKPDTIKGEDDAERLEKEPKKAVSDDSESKEASPGQQVEPQPKDETVDVEMKMNTSEDEEPMTELPRITNAVNGDLNGDLKASIGKPKSKPKPKAKLSSIIQKLIDSVPARLEQMSKTSAVIASTTTSSDRIGGGLSHALTHKVSPPSSATAAGRLVEYHTQHVSPRKRILREFEKVSLEDNGCVNNGSGGASSGGAGGKRSRAKGTSTSSPAGKASPMNLAPPQGKPSPSPGSSSSSTSPATLSTQPTRLNSSYSIHSLLGGSSGSGSSSFSSSGKKCGDHPAAIISNVHHPQHSMYQPSSSSYPRALLTSPKSPDVSGSNGGGGKSPSHTGTKKRSPPYSAGSPVDYGHSFYRDPYAGAGRPSTSGSASQDLSPPRSSPASPATTPRTVPKKTASIRREFASPSASSSSCPSPGDRSASPPERRHMQQQPHLQRSSPLHYYMYPPPPQVNGNGSAGSPTSAPPTSNSSAAAVAAAAAAAAAYIPSPSIYNPYISTLAALRHNPLWMHHYQTGASPLLSPHPQPGGSAAAAAAAAAARLSPQSAYHAFAYNGVGAAVAAAAAAAAFGQPAPSPHTHPHLAHPHQHPHPAALTTHHSPAHLATPKLTDSSTDQMSATSSHRTASTSPSSSSASASSSAATSGASSSAMFHTSSLRNEQSSDLPLNLSKH E8B846C0E2E575D4 o97169_drohy H hairless protein protein drohy Drosophila hydei (Fruit fly) MTDERNGNDNKSNISTSSKNNSNISPTSIANHSSSNSAGSSSNNERLIAEAAAKFLLKNGINGTNTAAAAAAATLLTAAAAAVTFQLPAAPELKPTTSATAANATNSTTSSSTATSTSNGTTPALSSTKTNSSSNSSSSSLIMATASATALVAGGAGVTAPKAPADVMAGVLDYSALNSPNATKSSKFVGTGNGSSSFDMGRHPISMRSNNSMSGYGGRLQFFKDGKFILELARAKDGDKGGWVSVPRKPFRTPSAATSATVTPTSAVTTTYPKNENSTSLSFSDDNSSIQSSPWQRDQPWKQTRPRRGISKELSLYYQRPRYNVLSQSARQAAMRKRRRPYEPTIATENHQSIFERVSAQENGDETLKAVEVDTKAPEIQSDDIKSETIKEQLTEGKAKSDSKACSSDNKDLNDSSAKAEATLEKMNTSEDEDAMSVTVDDGAPTTSDCLVNGNTSEVNGEPKAHTKCETASEKDTRRTGTPATKTTTMMTDIRLKKQKPRAKLNSIIQKLIDGVPARLEQLSKTPAVAAATASAVASTADRISSNSSGGGAIGSLSHSLAHKVSPPSSAAAASRLVEYHHHHQHVSPRKRILREFEKVSLEDNNGCVNNGSGVGGGISGGAGGKRSRAKGSTATSAVTTKSMPINLAPPQAKVLSTPTPSTTSVATATVATQPTRLNSSYSIHSLLGGSGSSSSKESHDQPAAIISNVHHQHHHSLYASSASYPRSMRNSPNSPEMGGSNGGKSPSHAATKKQRSPPYAAAGSPLPIDYGRRTPPDSYKLAQAHGQQPQHHSFYAPYSGATVSPHIPQAGGGTGTRDLLSPPRSSSASPREPAAASVAVSTPHRTVPKKTASIRREFASPTASNSNSSSTANSCPSPGERSTSSPERRHLQLQRGSPSAVAAAAAGSPLHYYMYPPTQLNGSGTASPHGIAPNATPTTSSTAVAAAAAAAAAAAYIPSVVSPSLYHPYISTLAALRHNPLWVQHYQAGASPLLPPPPPHPHSHPQTSSAAGSRLSPQSPYHPFSYNGVNAAAVAVAAAAAASAFGQPTSNPHLGHPTHPGALAHQNTPPTHLAEHRTHTPKLTDICTDQMSAASSHRTTISSLPAGSSVSVTSAGATQGGGGAGSGSTNSVQSAMFQNSSLRNELSSDLPLNLSKH 0B611D3739CF397F h-su_h_-1 1 2 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait hairless domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5 we tested the ability of this D.hydei Hairless domain for the binding of the D. melanogaster Su(H) protein in a yeast two-hybrid assay. As shown in Fig. 5, a very strong binding is observed which is in the range of the corresponding D. melanogaster Su(H)-binding domain. h-su_h_-2 1 3 unspecified role unspecified role bait bait hairless domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role prey prey physical association physical association 5 we tested the ability of this D.hydei Hairless domain for the binding of the D. melanogaster Su(H) protein in a yeast two-hybrid assay. As shown in Fig. 5, a very strong binding is observed which is in the range of the corresponding D. melanogaster Su(H)-binding domain.