IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. Neuron (0896-6273) 1999 imex curation Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. Mutation details from PMID:12972609 davie@hms.harvard.edu orchard Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. davie@hms.harvard.edu Accepted 2007-DEC-23 by ORCHARD. Neuron (0896-6273) 1999 orchard Only protein-protein interactions imex curation The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. Neuron (0896-6273) 1999 imex curation Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. Mutation details from PMID:12972609 davie@hms.harvard.edu orchard Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. davie@hms.harvard.edu Accepted 2007-DEC-23 by ORCHARD. Neuron (0896-6273) 1999 ORCHARD Only protein-protein interactions imex curation The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. Neuron (0896-6273) 1999 imex curation Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. Mutation details from PMID:12972609 davie@hms.harvard.edu orchard Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. davie@hms.harvard.edu Accepted 2007-DEC-22 by ORCHARD. Neuron (0896-6273) 1999 ORCHARD Only protein-protein interactions imex curation The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. The tyrosine kinase Abl and its substrate enabled collaborate with the receptor phosphatase Dlar to control motor axon guidance. Neuron (0896-6273) 1999 imex curation Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. Mutation details from PMID:12972609 davie@hms.harvard.edu orchard Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Wills Z., Bateman J., Korey CA., Comer A., Van Vactor D. Autoradiography followed by western blotting used for identifying proteins davie@hms.harvard.edu Accepted 2007-DEC-22 by ORCHARD. Neuron (0896-6273) 1999 ORCHARD Only protein-protein interactions imex curation larp_drome La-related protein 1 larp dLarp CG42551 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSKEETPSTGGAAPVATVAASGSATTSYANVVQNLETKKSGTSNTTTTTARATVDNKENQPNLKSFKSWSEEAAAAEAAGESPIVGSVSLAQTGEKRAAASGGDNTAENSSELDDNNDFVPVLSHHRRDRKKARKEKPRDQQPAGGRGDGQKPQQAGGRRVPGSSDTERKSVRPRAPRGGSPRKSAAGAAGAAGSNAGAVSSAVPGGPKVHKDRKDTSPSASIEGAGSSGNEAKSADGDQQAGEKGAAGAAPPPKRFIAAPPPKVNAWKISAKQSGSPKAGTSPLDKRVLQPKAQQQQQQTKQTASQNNNAQNTASNKKTQQQQQPQQGATTTTQTTTAAAAAATAAAAATAATTSAAAATSSAAAVPAATTETATTTTVDASQADALAKVVVKDKKKVNQKASDFSNVGDWPTLIGGTSGSGKATSNEPKRNPTKKQQSAKTAAAVAASSNTTSSEVATESNVAGTTSSSNSNPSSSTTTTNTTTNSQATTAPVASTSHDAKAQRDAEPAANSAAGTTGTAAGPALTKKIPKHKWRPLPIDLAKSSRPKPIGGRPNRRFSDDIADQRRPPRVYHDRQPGAGGAGVESRHPHAGRHPYGSRPATATSERVDSWRSSSSTTTAAFDEQRSGAAGGAGAAGTGVGSATRGPRRYRTPYRGGRQGRGGFSRQGPGRPTYRIPRHLLASGEYANYLPADAAGADSQSSYVLMGTHYFGNVPAAYIELDANSIKEAIKKQVEYYFSVDNLTGDFFLRRKMDPEGYIPVTLIASFHRVLALTTDVAVIVNAIKESDKLELFEGYKVRTKTTPTTWPITEVPEVNEGEPKAIGTLEQEQLEQNDGQEKLEEQTEADSPPPILTSAMATKPLNSIPPPPMPRNPQNLVPKMLQDKQQSRSSTIAALNSVNAISALTQQVEGGAAELAGHLSGLAESVKPKSTSTPDKRNAASAGNGAGSAAALVAEPEGIWKEVKRRSKTNAIKENATTPPQQQQPPLSQTLNNNNDNVKTNNTSSKSKSSSNNAPSNASSSATVCVTTNNASSATKATTKTTTTSTATTTTNNNIKSGNAAYSKTHSKSSSKTAAPPSAQCHAEKEELDFQFDEELMDPLPPGTGRINNFTENFSDDDESDYEFADRDINKLLIVAQVGRAPKHEGYDRTADFTSRTKITQDLENIINDGLVNYEEDLWTTTNVVADYKTVNVISQADFEKLAGGRNKSVLPPQVVPPPPPFEEDLDETLVGDTTLNSTLNNTLKSRRARFYAAPNSHSIDPRTPRKRKLRHTANPPVEAHVGWLLDTVEHRPRTTSMGSSAGTSPTASSYGSFGSSVPQSLPVFQHPSHALLKENNFTQQAYHKYHSRCLKERRRLGYGQSQEMNTLYRFWSFFLRENFNKSMYNEFRSLALEDAGNGFRYGLECLFRFFSYGLEKKFRPNIYQDFQDETIADYETGQLYGLEKFWAFLKYYKNGEKLEVQPKLAEYLKSFKNIEDFRVVEPEINEMLQGVGSLNPGRQLNRHRSVSESDGTAVIAAGGRRLNTTITNRSDYVGRLLQQQHQQQQQQQHHQYQQGYGGYNQQQNRRRTGSFGSTTVRIRSGSLGNKPQVANRNQGSQHELRRGGSNSGLAPHKRQQQQKPKPGAGSQTGSTRATTSAAATATTAASAATSTAATPAVTVSSGSSSSKK D865CDA3103B1429 lar_drome Tyrosine-protein phosphatase Lar Protein-tyrosine-phosphate phosphohydrolase dLAR Lar CG10443 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGLQMTAARPIAALSLLVLSLLTWTHPTIVDAAHPPEIIRKPQNQGVRVGGVASFYCAARGDPPPSIVWRKNGKKVSGTQSRYTVLEQPGGISILRIEPVRAGRDDAPYECVAENGVGDAVSADATLTIYEGDKTPAGFPVITQGPGTRVIEVGHTVLMTCKAIGNPTPNIYWIKNQTKVDMSNPRYSLKDGFLQIENSREEDQGKYECVAENSMGTEHSKATNLYVKVRRVPPTFSRPPETISEVMLGSNLNLSCIAVGSPMPHVKWMKGSEDLTPENEMPIGRNVLQLINIQESANYTCIAASTLGQIDSVSVVKVQSLPTAPTDVQISEVTATSVRLEWSYKGPEDLQYYVIQYKPKNANQAFSEISGIITMYYVVRALSPYTEYEFYVIAVNNIGRGPPSAPATCTTGETKMESAPRNVQVRTLSSSTMVITWEPPETPNGQVTGYKVYYTTNSNQPEASWNSQMVDNSELTTVSELTPHAIYTVRVQAYTSMGAGPMSTPVQVKAQQGVPSQPSNFRATDIGETAVTLQWTKPTHSSENIVHYELYWNDTYANQAHHKRISNSEAYTLDGLYPDTLYYIWLAARSQRGEGATTPPIPVRTKQYVPGAPPRNITAIATSSTTISLSWLPPPVERSNGRIIYYKVFFVEVGREDDEATTMTLNMTSIVLDELKRWTEYKIWVLAGTSVGDGPRSHPIILRTQEDVPGDPQDVKATPLNSTSIHVSWKPPLEKDRNGIIRGYHIHAQELRDEGKGFLNEPFKFDVVDTLEFNVTGLQPDTKYSIQVAALTRKGDGDRSAAIVVKTPGGVPVRPTVSLKIMEREPIVSIELEWERPAQTYGELRGYRLRWGVKDQALKEEMLSGPQMTKKRFDNLERGVEYEFRVAGSNHIGIGQETVKIFQTPEGTPGGPPSNITIRFQTPDVLCVTWDPPTREHRNGIITRYDVQFHKKIDHGLGSERNMTLRKAVFTNLEENTEYIFRVRAYTKQGAGPFSDKLIVETERDMGRAPMSLQAEATSEQTAEIWWEPVTSRGKLLGYKIFYTMTAVEDLDDWQTKTVGLTESADLVNLEKFAQYAVAIAARFKNGLGRLSEKVTVRIKPEDVPLNLRAHDVSTHSMTLSWSPPIRLTPVNYKISFDAMKVFVDSQGFSQTQIVPKREIILKHYVKTHTINELSPFTTYNVNVSAIPSDYSYRPPTKITVTTQMAAPQPMVKPDFYGVVNGEEILVILPQASEEYGPISHYYLVVVPEDKSNLHKIPDQFLTDDLLPGRNKPERPNAPYIAAKFPQRSIPFTFHLGSGDDYHNFTNRKLEREKRYRIFVRAVVDTPQKHLYTSSPFSEFLSLDMREAPPGERPHRPDPNWPAEPEVSVNRNKDEPEILWVVLPLMVSTFIVSTALIVLCVVKRRRQPCKTPDQAAVTRPLMAADLGAGPTPSDPVDMRRLNFQTPGMISHPPIPISEFANHIERLKSNDNQKFSQEYESIEPGQQFTWDNSNLEHNKSKNRYANVTAYDHSRVQLPAVEGVVGSDYINANYCDGYRKHNAYVATQGPLQETFVDFWRMCWELKTATIVMMTRLEERTRIKCDQYWPTRGTETYGQIFVTITETQELATYSIRTFQLCRQGFNDRREIKQLQFTAWPDHGVPDHPAPFLQFLRRCRALTPPESGPVIVHCSAGVGRTGCYIVIDSMLERMKHEKIIDIYGHVTCLRAQRNYMVQTEDQYIFIHDAILEAIICGVTEVPARNLHTHLQKLLITEPGETISGMEVEFKKLSNVKMDSSKFVTANLPCNKHKNRLVHILPYESSRVYLTPIHGIEGSDYVNASFIDGYRYRSAYIAAQGPVQDAAEDFWRMLWEHNSTIVVMLTKLKEMGREKCFQYWPHERSVRYQYYVVDPIAEYNMPQYKLREFKVTDARDGSSRTVRQFQFIDWPEQGVPKSGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRSGVFITLSIVLERMQYEGVLDVFQTVRILRSQRPAMVQTEDQYHFCYRAALEYLGSFDNYTN DIP protein P16621 original sequence version: 161 DIP protein P16621 original sequence version: 123 DF1F676A94050F2B ptp10_drome Tyrosine-protein phosphatase 10D Receptor-linked protein-tyrosine phosphatase 10D Ptp10D CG1817 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLYQLSKATTRIRLKRQKAVPQHRWLWSLAFLAAFTLKDVRCADLAISIPNNPGLDDGASYRLDYSPPFGYPEPNTTIASREIGDEIQFSRALPGTKYNFWLYYTNFTHHDWLTWTVTITTAPDPPSNLSVQVRSGKNAIILWSPPTQGSYTAFKIKVLGLSEASSSYNRTFQVNDNTFQHSVKELTPGATYQVQAYTIYDGKESVAYTSRNFTTKPNTPGKFIVWFRNETTLLVLWQPPYPAGIYTHYKVSIEPPDANDSVLYVEKEGEPPGPAQAAFKGLVPGRAYNISVQTMSEDEISLPTTAQYRTVPLRPLNVTFDRDFITSNSFRVLWEAPKGISEFDKYQVSVATTRRQSTVPRSNEPVAFFDFRDIAEPGKTFNVIVKTVSGKVTSWPATGDVTLRPLPVRNLRSINDDKTNTMIITWEADPASTQDEYRIVYHELETFNGDTSTLTTDRTRFTLESLLPGRNYSLSVQAVSKKMESNETSIFVVTRPSSPIIEDLKSIRMGLNISWKSDVNSKQEQYEVLYSRNGTSDLRTQKTKESRLVIKNLQPGAGYELKVFAVSHDLRSEPHAYFQAVYPNPPRNMTIETVRSNSVLVHWSPPESGEFTEYSIRYRTDSEQQWVRLPSVRSTEADITDMTKGEKYTIQVNTVSFGVESPVPQEVNTTVPPNPVSNIIQLVDSRNITLEWPKPEGRVESYILKWWPSDNPGRVQTKNVSENKSADDLSTVRVLIGELMPGVQYKFDIQTTSYGILSGITSLYPRTMPLIQSDVVVANGEKEDERDTITLSYTPTPQSSSKFDIYRFSLGDAEIRDKEKLANDTDRKVTFTGLVPGRLYNITVWTVSGGVASLPIQRQDRLYPEPITQLHATNITDTEISLRWDLPKGEYNDFDIAYLTADNLLAQNMTTRNEITISDLRPHRNYTFTVVVRSGTESSVLRSSSPLSASFTTNEAVPGRVERFHPTDVQPSEINFEWSLPSSEANGVIRQFSIAYTNINNLTDAGMQDFESEEAFGVIKNLKPGETYVFKIQAKTAIGFGPEREYRQTMPILAPPRPATQVVPTEVYRSSSTIQIRFRKNYFSDQNGQVRMYTIIVAEDDAKNASGLEMPSWLDVQSYSVWLPYQAIDPYYPFENRSVEDFTIGTENCDNHKIGYCNGPLKSGTTYRVKVRAFTGADKFTDTAYSFPIQTDQDNTSLIVAITVPLTIILVLLVTLLFYKRRRNNCRKTTKDSRANDNMSLPDSVIEQNRPILIKNFAEHYRLMSADSDFRFSEEFEELKHVGRDQPCTFADLPCNRPKNRFTNILPYDHSRFKLQPVDDDEGSDYINANYVPGHNSPREFIVTQGPLHSTRDDFWRMCWESNSRAIVMLTRCFEKGREKCDQYWPNDTVPVFYGDIKVQILNDSHYADWVMTEFMLCRGSEQRILRHFHFTTWPDFGVPNPPQTLVRFVRAFRDRIGAEQRPIVVHCSAGVGRSGTFITLDRILQQINTSDYVDIFGIVYAMRKERVWMVQTEQQYICIHQCLLAVLEGKENIVGPAREMHDNEGYEGQQVQLDENGDVVATIEGHLSHHDLQQAEAEAIDDENAAILHDDQQPLTSSFTGHHTHMPPTTSMSSFGGGGGGHTNVDAPDRXHSVVNQSDNNNSVVIVLVDNKPSSMICKDSKGGNIDVLESQQQQQQQQQQQPNQGGHNITTISAINGYNTLQHRRKSQLITFSSSSCDIKNSLSHEYINGSNGSAANGPPSSGSGSGSGPGSNRASRANVRLSFAEEDVMILPQNHSQQSNHQDDEVFTRRRSLLEVEIGVEVGEDGELAPHEMEEDLEEEDEDEELYMHDEFETHIDTKSNNANDDSGGGSYEDSHALHSSLGGSNRNSLEKDDDDIEVDVISTDVSCYDQLLGSSCNTRNGDDDDIATLVGDGDYSTTKLSKASRLSGAGVGGLVVSGGGGGTAIGGGIAVNGGGVLGNGVGSEAGGGIIYANPFMDDEGIAESGM 76FAAA08565AE8DA ena_drome Protein enabled CG15112 ena enb protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAMKKLYAKTSFTSKKPSSAANSTPILAYHQQQHQQPGNGICEFQVVAPGHSGELMIRRSQSMHHKMSPPVGGLGSKSEYYSIEELQELDLLDYRHPMYHHYQQQELRQRYHEHEQLVLQLPKATSPKAGPIYEAPQRSQQQQDQMLYVPTAAQRDSSSSAAATSIASSSTLTSSPSPSSSSSLIFSTLRKCVSPSNPSVNPNQPSKTQPSKLGCSMSFSIRTTTATAATAAAANAATATLSTQQQQQQAQQQHKQHLYSNIHHYLIRQQQQKQHYTLQRRHNSVKDKFIGGITTIFAEQSIIGARASVMVYDDNQKKWVPSGSSSGLSKVQIYHHQQNNTFRVVGRKLQDHEVVINCSILKGLKYNQATATFHQWRDSKFVYGLNFSSQNDAENFARAMMHALEVLSGRVANNPGGPPTNGNGYEEDMGYRTMTSEDAAILRQNNSIGGHVTPSAQTPTSQTNQNNIPQSPPTPQGHHRTSSAPPAPQPQQQQQQQQAQQMGQPGSHYGPTGNGPTSNGLPQQVNSQIPPAPQQQPQQQQFQQQQQQQQYQQMVQAGYAPSQQYQQPHYVLSNSNPNLTVHQYPTQQAQQQPPQAPQPPLQNGGMYMVGHGHLPSSASANSVVYASQQQMLPQAHPQAPQAPTMPGPGYGGPPVPPPQQQAENPYGQVPMPPPMNPSQQQQPGQVPLNRMSSQGGPGGPPAPAPPPPPPSFGGAAGGGPPPPAPPQMFNGAPPPPAMGGGPPPAPPAPPAMGGGPPPAPGGPGAPPPPPPPPGLGGAPKKEDPQADLMGSLASQLQQIKLKKNKVTTSAPENSGSSTSSGGSGNYGTIGRSSNGMASMMDEMAKTLARRRAQAEKKDPDPEAEVKKRPWEKSNTLPHKLSGGAGSGSAGSGHEGANGNSGGAGSNTTNSGGESPRPMRKRFGSASEETILKVNGDGLSLALSNGDLDTLKAEIVREMRLEIQKVKNEIIDAIKSEFNRR 7B084A0AEC098297 abl_drome Tyrosine-protein kinase Abl Protein abelson Abl ABL-1 Dash CG4032 D-ash protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGAQQGKDRGAHSGGGGSGAPVSCIGLSSSPVASVSPHCISSSSGVSSAPLGGGSTLRGSRIKSSSSGVASGSGSGGGGGGSGSGLSQRSGGHKDARCNPTVGLNIFTEHNEALLQSRPLPHIPAGSTAASLLADAAELQQHQQDSGGLGLQGSSLGGGHSSTTSVFESAHRWTSKENLLAPGPEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSDSGNVGWVPSNYVTPLNSLEKHSWYHGPISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRISEDPDGKVFVTQEAKFNTLAELVHHHSVPHEGHGLITPLLYPAPKQNKPTVFPLSPEPDEWEICRTDIMMKHKLGGGQYGEVYEAVWKRYGNTVAVKTLKEDTMALKDFLEEAAIMKEMKHPNLVQLIGVCTREPPFYIITEFMSHGNLLDFLRSAGRETLDAVALLYMATQIASGMSYLESRNYIHRDLAARNCLVGDNKLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGVLLWEIATYGMSPYPAIDLTDVYHKLDKGYRMERPPGCPPEVYDLMRQCWQWDATDRPTFKSIHHALEHMFQESSITEAVEKQLNANATSASSSAPSTSGVATGGGATTTTAASGCASSSSATASLSLTPQMVKKGLPGGQALTPNAHHNDPHQQQASTPMSETGSTSTKLSTFSSQGKGNVQMRRTTNKQGKQAPAPPKRTSLLSSSRDSTYREEDPANARCNFIDDLSTNGLARDINSLTQRYDSETDPAADPDTDATGDSLEQSLSQVIAAPVTNKMQHSLHSGGGGGGIGPRSSQQHSSFKRPTGTPVMGNRGLETRQSKRSQLHSQAPGPGPPSTQPHHGNNGVVTSAHPITVGALDVMNVKQVVNRYGTLPKGARIGAYLDSLEDSSEAAPALPATAPSLPPANGHATPPAARLNPKASPIPPQQMIRSNSSGGVTMQNNAAASLNKLQRHRTTTEGTMMTFSSFRAGGSSSSPKRSASGVASGVQPALANLEFPPPPLDLPPPPEEFEGGPPPPPPAPESAVQAIQQHLHAQLPNNGNISNGNGTNNNDSSHNDVSNIAPSVEEASSRFGVSLRKREPSTDSCSSLGSPPEDLKEKLITEIKAAGKDTAPASHLANGSGIAVVDPVSLLVTELAESMNLPKPPPQQQQKLTNGNSTGSGFKAQLKKVEPKKMSAPMPKAEPANTIIDFKAHLRRVDKEKEPATPAPAPATVAVANNANCNTTGTLNRKEDGSKKFSQAMQKTEIKIDVTNSNVEADAGAAGEGDLGKRRSTGSINSLKKLWEQQPPAPDYATSTILQQQPSVVNGGGTPNAQLSPKYGMKSGAINTVGTLPAKLGNKQPPAAPPPPPPNCTTSNSSTTSISTSSRDCTSRQQASSTIKTSHSTQLFTDDEEQSHTEGLGSGGQGSADMTQSLYEQKPQIQQKPAVPHKPTKLTIYATPIAKLTEPASSASSTQISRESILELVGLLEGSLKHPVNAIAGSQWLQLSDKLNILHNSCVIFAENGAMPPHSKFQFRELVTRVEAQSQHLRSAGSKNVQDNERLVAEVGQSLRQISNALNR 14287B02CC8FE86B ptp69_drome Tyrosine-protein phosphatase 69D Protein-tyrosine-phosphate phosphohydrolase Ptp69D DPTP CG10975 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALLYRRMSMLLNIILAYIFLCAICVQGSVKQEWAEIGKNVSLECASENEAVAWKLGNQTINKNHTRYKIRTEPLKSNDDGSENNDSQDFIKYKNVLALLDVNIKDSGNYTCTAQTGQNHSTEFQVRPYLPSKVLQSTPDRIKRKIKQDVMLYCLIEMYPQNETTNRNLKWLKDGSQFEFLDTFSSISKLNDTHLNFTLEFTEVYKKENGTYKCTVFDDTGLEITSKEITLFVMEVPQVSIDFAKAVGANKIYLNWTVNDGNDPIQKFFITLQEAGTPTFTYHKDFINGSHTSYILDHFKPNTTYFLRIVGKNSIGNGQPTQYPQGITTLSYDPIFIPKVETTGSTASTITIGWNPPPPDLIDYIQYYELIVSESGEVPKVIEEAIYQQNSRNLPYMFDKLKTATDYEFRVRACSDLTKTCGPWSENVNGTTMDGVATKPTNLSIQCHHDNVTRGNSIAINWDVPKTPNGKVVSYLIHLLGNPMSTVDREMWGPKIRRIDEPHHKTLYESVSPNTNYTVTVSAITRHKKNGEPATGSCLMPVSTPDAIGRTMWSKVNLDSKYVLKLYLPKISERNGPICCYRLYLVRINNDNKELPDPEKLNIATYQEVHSDNVTRSSAYIAEMISSKYFRPEIFLGDEKRFSENNDIIRDNDEICRKCLEGTPFLRKPEIIHIPPQGSLSNSDSELPILSEKDNLIKGANLTEHALKILESKLRDKRNAVTSDENPILSAVNPNVPLHDSSRDVFDGEIDINSNYTGFLEIIVRDRNNALMAYSKYFDIITPATEAEPIQSLNNMDYYLSIGVKAGAVLLGVILVFIVLWVFHHKKTKNELQGEDTLTLRDSLSRALFGRRNHNHSHFITSGNHKGFDAGPIHRLDLENAYKNRHKDTDYGFLREYEMLPNRFSDRTTKNSDLKENACKNRYPDIKAYDQTRVKLAVINGLQTTDYINANFVIGYKERKKFICAQGPMESTIDDFWRMIWEQHLEIIVMLTNLEEYNKAKCAKYWPEKVFDTKQFGDILVKFAQERKTGDYIERTLNVSKNKANVGEEEDRRQITQYHYLTWKDFMAPEHPHGIIKFIRQINSVYSLQRGPILVHCSAGVGRTGTLVALDSLIQQLEEEDSVSIYNTVCDLRHQRNFLVQSLKQYIFLYRALLDTGTFGNTDICIDTMASAIESLKRKPNEGKCKLEVEFEKLLATADEISKSCSVGENEENNMKNRSQEIIPYDRNRVILTPLPMRENSTYINASFIEGYDNSETFIIAQDPLENTIGDFWRMISEQSVTTLVMISEIGDGPRKCPRYWADDEVQYDHILVKYVHSESCPYYTRREFYVTNCKIDDTLKVTQFQYNGWPTVDGEVPEVCRGIIELVDQAYNHYKNNKNSGCRSPLTVHCSLGTDRSSIFVAMCILVQHLRLEKCVDICATTRKLRSQRTGLINSYAQYEFLHRAIINYSDLHHIAESTLD 61214ADC778D319A lar-abl 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cytoplasmic region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association 2 Endogenous Abl protein expressed in S2 cell lysates associates specifically with GST fusion proteins that contain the cytoplasmic domain of Dlar, as visualized by Western blot with anti-Abl antibodies lar-abl-1 4 9 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined d2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2B Purified, recombinant Drosophila Abl B or v Abl C binds to GST Dlar in vitro, as assessed by a pull down assay in which dAbl protein has been prelabeled with g32P ATP before mixing with the fusion proteins. v-abl is not adequately defined. ena-lar 1 8 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined d2 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 5 We exposed equal amounts of different GST PTP fusion proteins to Drosophila S2 cell lysates and found that endogenous Ena protein associates with a Dlar full length cytoplasmic domain (GST Dlar D1 D2) or with D2 alone but not comparably with wild type D1 ena-lars-1 4 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined d1d2 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1533760]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 1452-1452, sequence length 1403 [EBI-1533760]1452-2030 ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction 5 Therefore, we repeated the GST pull down assays using purified, recombinant Ena protein in the absence of other Drosophila proteins. Purified Ena bound to the Dlar cytoplasmic domain (Figure 5B) lar-abl-2 3 9 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 enzyme target enzyme target substrate substrate unspecified role unspecified role d2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2E The phosphorylation of GST fusion proteins by purified d-Abl is shown following a kinase assay with gamma32P-ATP v-abl is not adequately defined. Phosphorylation of Larp ptp69d-abl 3 10 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined d2 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1533996]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 1480-1480, sequence length 1462 [EBI-1533996]1191-1480 ecoli Escherichia coli (strain K12) 9 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 2F The phosphorylation of GST fusion proteins by purified d-Abl is shown following a kinase assay with gamma32P-ATP v-abl is not adequately defined. Phosphorylation of PTP69d ena-lar-1 2 6 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined d1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cys1670ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain C S Wed Jul 06 17:06:07 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'cys ser' ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection dephosphorylation dephosphorylation reaction dephosphorylation 6 Abl and Ena Are Substrates for the Dlar Phosphatase In Vitro Loss of phosphate group. lar-abl-3 2 9 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 enzyme enzyme unspecified role unspecified role P16621:p.Cys1670Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:30:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys1670ser' Tue Jun 30 18:05:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys1670Ser' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined d1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) dephosphorylation dephosphorylation reaction dephosphorylation 6 Abl and Ena Are Substrates for the Dlar Phosphatase In Vitro Loss of phosphorylation. ena-ptp10d 1 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association 5 Purified, recombinant Ena (arrow) binds to GST�Dlar D1�D2 (lane 2), and binds weakly to GST�DPTP10D Weak interaction was observed. ena-ptp69d-1 1 8 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined d2 region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) physical association physical association 5 Ena bound effectively to the D2 domain of DPTP69D