IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A cellular cofactor for the constitutive transport element of type D retrovirus. A cellular cofactor for the constitutive transport element of type D retrovirus. Science (0036-8075) 1997 Tang H., Gaietta GM., Fischer WH., Ellisman MH., Wong-Staal F. RNA selection : biotin labeled RNA were synthesized using Biotin-rUTP and incubated with streptavidin-conjugated agarose beads. The beads were then incubated with a cell lysate. table 1, fig 1a fwonfstaal@ucsd.edu in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa western blot western blot Immuno blot Immuno blot Tang H., Gaietta GM., Fischer WH., Ellisman MH., Wong-Staal F. RNA selection : biotin labeled RNA were synthesized using Biotin-rUTP and incubated with streptavidin-conjugated agarose beads. The beads were then incubated with a cell lysate. table 1, fig 1a fwonfstaal@ucsd.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 1997 A cellular cofactor for the constitutive transport element of type D retrovirus. A cellular cofactor for the constitutive transport element of type D retrovirus. Science (0036-8075) 1997 Tang H., Gaietta GM., Fischer WH., Ellisman MH., Wong-Staal F. RNA selection : biotin labeled RNA were synthesized using Biotin-rUTP and incubated with streptavidin-conjugated agarose beads. The beads were then incubated with a cell lysate. table 1, fig 1a fwonfstaal@ucsd.edu human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Tang H., Gaietta GM., Fischer WH., Ellisman MH., Wong-Staal F. RNA selection : biotin labeled RNA were synthesized using Biotin-rUTP and incubated with streptavidin-conjugated agarose beads. The beads were then incubated with a cell lysate. table 1, fig 1a fwonfstaal@ucsd.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 1997 A cellular cofactor for the constitutive transport element of type D retrovirus. A cellular cofactor for the constitutive transport element of type D retrovirus. Science (0036-8075) 1997 Tang H., Gaietta GM., Fischer WH., Ellisman MH., Wong-Staal F. RNA selection : biotin labeled RNA were synthesized using Biotin-rUTP and incubated with streptavidin-conjugated agarose beads. The beads were then incubated with a cell lysate. table 1, fig 1a fwonfstaal@ucsd.edu in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Tang H., Gaietta GM., Fischer WH., Ellisman MH., Wong-Staal F. RNA selection : biotin labeled RNA were synthesized using Biotin-rUTP and incubated with streptavidin-conjugated agarose beads. The beads were then incubated with a cell lysate. table 1, fig 1a fwonfstaal@ucsd.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. Science (0036-8075) 1997 cte_mpmv constitutive transport element (CTE) in the 3'UTR of Simian Mason-Pfizer virus rna ribonucleic acid RNA rna mpmv Mason-Pfizer monkey virus (MPMV) Simian Mason-Pfizer virus MPMV The CTE sequence is found in the 3' UTR of the viral RNA and corresponds to the 8005-8240 of M12349 EMBL entry. Cis-acting sequence interacting with cellular nuclear export proteins to regulate the viral RNA splicing and export. dhx9_human ATP-dependent RNA helicase A DHX9 DDX9 NDH2 DExH-box helicase 9 RNA helicase A LKP Nuclear DNA helicase II DEAH box protein 9 Leukophysin protein protein human Homo sapiens Human MGDVKNFLYAWCGKRKMTPSYEIRAVGNKNRQKFMCEVQVEGYNYTGMGNSTNKKDAQSNAARDFVNYLVRINEIKSEEVPAFGVASPPPLTDTPDTTANAEGDLPTTMGGPLPPHLALKAENNSEVGASGYGVPGPTWDRGANLKDYYSRKEEQEVQATLESEEVDLNAGLHGNWTLENAKARLNQYFQKEKIQGEYKYTQVGPDHNRSFIAEMTIYIKQLGRRIFAREHGSNKKLAAQSCALSLVRQLYHLGVVEAYSGLTKKKEGETVEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEILEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERGEEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAGIRGISHVIVDEIHERDINTDFLLVVLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFVPPPKDKKKKDKDDDGGEDDDANCNLICGDEYGPETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLLRLGGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCIFYVGDAICTIAAATCFPEPFINEGKRLGYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEAEIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQVFTNTGPDNNLDVVISLLAFGVYPNVCYHKEKRKILTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGEKIRTRAISAKGMTLVTPLQLLLFASKKVQSDGQIVLVDDWIKLQISHEAAACITGLRAAMEALVVEVTKQPAIISQLDPVNERMLNMIRQISRPSAAGINLMIGSTRYGDGPRPPKMARYDNGSGYRRGGSSYSGGGYGGGYSSGGYGSGGYGGSANSFRAGYGAGVGGGYRGVSRGGFRGNSGGDYRGPSGGYRGSGGFQRGGGRGAYGTGYFGQGRGGGGY DIP protein Q08211 original sequence version: 114 DIP protein Q08211 original sequence version: 177 A607DA8F4C4B217A cte_srv1 constitutive transport element (CTE) in the 3'UTR of Simian SRV-1 type D retrovirus rna ribonucleic acid RNA rna srv1 Simian retrovirus SRV-1 The CTE sequence is found in the 3' UTR of the viral RNA corresponding to 7620-7859 fragment of the M11841 EMBL entry. Cis-acting sequence interacting with cellular nuclear export proteins to regulate the viral RNA splicing and export. cte-dhx9-8 3 4 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association Export of unspliced viral RNA. cte-dhx9-9 3 5 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 6 unspecified role unspecified role bait bait in vitro In vitro physical association physical association Export of unspliced viral RNA. cte-dhx9-12 1 5 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait in vitro In vitro physical association physical association Export of unspliced viral RNA. cte-dhx9-11 2 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Export of unspliced viral RNA.