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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
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        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Lysosome membrane lipid microdomains: novel regulators of chaperone-mediated autophagy.</fullName>
        </names>
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          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Lysosome membrane lipid microdomains: novel regulators of chaperone-mediated autophagy.</attribute>
            <attribute name="journal" nameAc="MI:0885">EMBO J. (0261-4189)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2006</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Kaushik S., Massey AC., Cuervo AM.</attribute>
            <attribute name="imex-exported">2009-01-08</attribute>
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        </bibref>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="10116">
            <names>
              <shortLabel>rat-liver</shortLabel>
              <fullName>rattus norvegicus liver</fullName>
              <alias type="synonym" typeAc="MI:1041">Rat</alias>
            </names>
            <tissue>
              <names>
                <shortLabel>liver</shortLabel>
                <fullName>liver</fullName>
              </names>
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        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>density sedimentation</shortLabel>
            <fullName>cosedimentation through density gradient</fullName>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
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            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
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          <attribute name="publication year" nameAc="MI:0886">2006</attribute>
          <attribute name="imex-exported">2009-01-08</attribute>
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      <interactor id="2">
        <names>
          <shortLabel>lamp2_rat</shortLabel>
          <fullName>Lysosome-associated membrane glycoprotein 2</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">Lysosomal membrane glycoprotein type B</alias>
          <alias type="gene name synonym" typeAc="MI:0302">LGP-96</alias>
          <alias type="gene name synonym" typeAc="MI:0302">LGP-110</alias>
          <alias type="gene name synonym" typeAc="MI:0302">CD107 antigen-like family member B</alias>
          <alias type="gene name" typeAc="MI:0301">Lamp2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Lamp-2</alias>
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            <shortLabel>protein</shortLabel>
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        </interactorType>
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            <shortLabel>rat</shortLabel>
            <fullName>Rattus norvegicus (Rat)</fullName>
            <alias type="synonym" typeAc="MI:1041">Rat</alias>
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        </organism>
        <sequence>MRLLSPVTGSKLVLLFLFLGAVRSDALKLNLTDSKGTCLYAEWEMNFTITYEALKVNETVTITVPDKVTYNGSSCGDDKNGAKIMIQYGSTLSWAVNFTKEASQYFINNITLSYNTNDTKTFPGAVPKGILTVIIPVGSQLPLGVIFKCSSVLTFNLSPVVQHYWGIHLQAFVQNGTVSKHEQVCKEDKTATTVAPIIHTTVPSPTTTLTPTSIPVPTPTVGNYTISNGNATCLLATMGLQLNITEEKVPFIFNINPAITNFTGSCQPQTAQLRLNNSQIKYLDFIFAVKNEKRFYLKEVNVNMYLANGSAFHVSNNNLSFWDAPLGSSYMCNKEQVVSVSRTFQINTFNLKVQPFNVTKGEYSTAQDCSADEDNFLVPIAVGAALGGVLILVLLAYFIGLKRHHTGYEQF</sequence>
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          <attribute name="crc64">001CFBA548AD3869</attribute>
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      </interactor>
      <interactor id="3">
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          <shortLabel>flot1_rat</shortLabel>
          <fullName>Flotillin-1</fullName>
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          <alias type="gene name synonym" typeAc="MI:0302">Reg2</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Reggie-2</alias>
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          </names>
        </organism>
        <sequence>MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQCLSEIEMAKAQRDYELKKATYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYRLERLAEAEKAQLIMQAEAEAESVRMRGEAEAFAVGARARAEAEQMAKKAEAFQMYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILSRLPESVERLTGVSISQVNHNKPLRTA</sequence>
        <attributeList>
          <attribute name="crc64">B3B54DDB81705EAF</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-11041-1">
        <names>
          <shortLabel>flot1-lamp2</shortLabel>
        </names>
        <xref>
          <primaryRef db="mint" dbAc="MI:0471" id="MINT-2982166" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8615788" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-11041-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-2982167" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>2</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>western blot</shortLabel>
                  <fullName>western blot</fullName>
                  <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
                  <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>subcellular prep</shortLabel>
                  <fullName>subcellular preparation</fullName>
                  <alias type="synonym" typeAc="MI:1041">subcellular prep</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0372" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537724" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
              <experimentalPreparation>
                <names>
                  <shortLabel>physiological level</shortLabel>
                  <fullName>physiological level</fullName>
                  <alias type="go synonym" typeAc="MI:0303">endogenous level</alias>
                  <alias type="go synonym" typeAc="MI:0303">endogenous</alias>
                  <alias type="synonym" typeAc="MI:1041">endogenous</alias>
                  <alias type="synonym" typeAc="MI:1041">endogenous level</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0222" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537793" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <stoichiometry value="1"/>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
          <participant id="6">
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-2982168" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <interactorRef>3</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>western blot</shortLabel>
                  <fullName>western blot</fullName>
                  <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
                  <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>subcellular prep</shortLabel>
                  <fullName>subcellular preparation</fullName>
                  <alias type="synonym" typeAc="MI:1041">subcellular prep</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0372" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537724" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
              <experimentalPreparation>
                <names>
                  <shortLabel>physiological level</shortLabel>
                  <fullName>physiological level</fullName>
                  <alias type="go synonym" typeAc="MI:0303">endogenous level</alias>
                  <alias type="go synonym" typeAc="MI:0303">endogenous</alias>
                  <alias type="synonym" typeAc="MI:1041">endogenous</alias>
                  <alias type="synonym" typeAc="MI:1041">endogenous level</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0222" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537793" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <stoichiometry value="1"/>
            <attributeList>
              <attribute name="comment" nameAc="MI:0612">Stoichiometry: 1.0</attribute>
            </attributeList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>colocalization</shortLabel>
            <fullName>colocalization</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0403" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608832" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">F1</attribute>
          <attribute name="remark-internal">EMBO-Journal</attribute>
          <attribute name="comment" nameAc="MI:0612">homomint</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>