IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Plant Cell (1040-4651) 2006 Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Plant Cell (1040-4651) 2006 Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). arath-leaf Arabidopsis thaliana leaf leaf leaf fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Plant Cell (1040-4651) 2006 Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). arath-leaf Arabidopsis thaliana leaf leaf leaf molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Plant Cell (1040-4651) 2006 Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Plant Cell (1040-4651) 2006 Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Song J., Lee MH., Lee GJ., Yoo CM., Hwang I. The authors supplied details of the anti-clathrin antibody This study was carried out in arabidopsis leaf protoplasts. TAIR identifiers were mapped to UniProt accession numbers using an index provided by Dr P Kersey. Arabidopsis clathrin was identified by sequence similarity search using soyabean clathrin (GenBank:U42608) as a query sequence. ihhwang@postech.ac.kr Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. The antibody used to detect arabidospsis clathrin protein was raised against soyabean clathrin protein (UniProt accession number Q39834). Plant Cell (1040-4651) 2006 ap2a1_arath AP-2 complex subunit alpha-1 At5g22770 Adaptor protein complex AP-2 subunit alpha-1 Alpha-adaptin 1 Clathrin assembly protein complex 2 alpha large chain 1 MDJ22.19 ALPHA-ADR Adaptor-related protein complex 2 subunit alpha-1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTGMRGLSVFISDVRNCQNKEAERLRVDKELGNIRTCFKNEKVLTPYKKKKYVWKMLYIHMLGYDVDFGHMEAVSLISAPKYPEKQVGYIVTSCLLNENHDFLKLAINTVRNDIIGRNETFQCLALTLVGNIGGRDFAESLAPDVQKLLISSSCRPLVRKKAALCLLRLFRKNPDAVNVDGWADRMAQLLDERDLGVLTSSTSLLVALVSNNHEAYSSCLPKCVKILERLARNQDVPQEYTYYGIPSPWLQVKAMRALQYFPTIEDPSTRKALFEVLQRILMGTDVVKNVNKNNASHAVLFEALSLVMHLDAEKEMMSQCVALLGKFISVREPNIRYLGLENMTRMLMVTDVQDIIKKHQSQIITSLKDPDISIRRRALDLLYGMCDVSNAKDIVEELLQYLSTAEFSMREELSLKAAILAEKFAPDLSWYVDVILQLIDKAGDFVSDDIWFRVVQFVTNNEDLQPYAASKAREYLDKIAIHETMVKVSAYILGEYGHLLARQPGCSASELFSILHEKLPTISTPTIPILLSTYAKLLMHAQPPDPELQKKVWAVFKKYESCIDVEIQQRAVEYFELSKKGPAFMDVLAEMPKFPERQSSLIKKAENVEDTADQSAIKLRAQQQPSNAMVLADQQPVNGAPPPLKVPILSGSTDPESVARSLSHPNGTLSNIDPQTPSPDLLSDLLGPLAIEAPPGAVSNEQHGPVGAEGVPDEVDGSAIVPVEEQTNTVELIGNIAERFHALCLKDSGVLYEDPHIQIGIKAEWRGHHGRLVLFMGNKNTSPLTSVQALILPPAHLRLDLSPVPDTIPPRAQVQSPLEVMNIRPSRDVAVLDFSYKFGANVVSAKLRIPATLNKFLQPLQLTSEEFFPQWRAISGPPLKLQEVVRGVRPLALPEMANLFNSFHVTICPGLDPNPNNLVASTTFYSESTGAILCLARIETDPADRTQLRMTVGTGDPTLTFELKEFIKEQLITVPMGSRALVPAAGPAPPVAQPPSPAALADDPGAMLAGLL 01E16E8A1DB0280D vti11_arath Vesicle transport v-SNARE 11 Vesicle transport v-SNARE protein VTI1a Vesicle soluble NSF attachment protein receptor VTI1a VTI11 VTI1A Protein SHOOT GRAVITROPISM 4 SGR4 ZIG ZIG1 MUL8_190 At5g39510 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSDVFDGYERQYCELSASLSKKCSSAISLDGEQKKQKLSEIKSGLENAEVLIRKMDLEARTLPPNLKSSLLVKLREFKSDLNNFKTEVKRITSGQLNAAARDELLEAGMADTKTASADQRARLMMSTERLGRTTDRVKDSRRTMMETEEIGVSILQDLHGQRQSLLRAHETLHGVDDNIGKSKKILTDMTRRMNKNKWTIGAIIIALIAAIFIILYFKLTK AFC1E86167981ED0 epn1_arath Clathrin interactor EPSIN 1 At5g11710 EPSINR1 EPSIN-related 1 T22P22.100 EPSIN1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDFMKVFDQTVREIKREVNLKVLKVPEMEQKVLDATDNEPWGPHGTALAEIAQATKKFSECQMVMSVLWTRLSETGKDWRYVYKALAVIDYLISNGSERAVDEIIEHTYQISSLTSFEYVEPNGKDVGINVRKKAENIVALLNNKEKISEIRDKAVANRNKYVGLSSTGITYKSGSSASFGGSFQSGSSNFDSYKDRDSREDKNDYESFQKSRRGVKTEEQSYTSKKSFSRYGSTDHDNLSSGKKSPDSAKHRSYVSAAPSNNDDDFDDFDPRGTSSNKPSTGSANQVDLFGGDLIGDFLDSGPTETSSTNNNENFQEADLFADAAFVSASAQGAEFGSQTQKQVDLFSASEPSVTVSSAPPTVDLFASSESVVSPEAKISIPESMATPNIVDPFAAVPMDNFDGSDPFGAFTSHSASVSTGPQAPSVHGSATNTTSPLSFADSKPQHLQKKDPFQVKSGIWADSLSRGLIDLNITAPKKASLADVGVVGDLSNEDGNKASAASYYSGWSMGAGSGLGKTGLYSTQQQQQQQQQQQAPDISDDFFSSLSNQRYQSGGFKQ 29272781B09D2629 clah1_arath Clathrin heavy chain 1 CHC1 F9F8.6 F11B9.30 At3g11130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAAANAPIIMKEVLTLPSVGIGQQFITFTNVTMESDKYICVRETAPQNSVVIIDMNMPMQPLRRPITADSALMNPNSRILALKAQVPGTTQDHLQIFNIEAKAKLKSHQMPEQVAFWKWITPKMLGLVTQTSVYHWSIEGDSEPVKMFDRTANLANNQIINYKCSPNEKWLVLIGIAPGSPERPQLVKGNMQLFSVDQQRSQALEAHAASFAQFKVPGNENPSILISFASKSFNAGQITSKLHVIELGAQPGKPSFTKKQADLFFPPDFADDFPVAMQVSHKFNLIYVITKLGLLFVYDLETASAIYRNRISPDPIFLTSEASSVGGFYAINRRGQVLLATVNEATIIPFISGQLNNLELAVNLAKRGNLPGAENLVVQRFQELFAQTKYKEAAELAAESPQGILRTPDTVAKFQSVPVQAGQTPPLLQYFGTLLTRGKLNSYESLELSRLVVNQNKKNLLENWLAEDKLECSEELGDLVKTVDNDLALKIYIKARATPKVVAAFAERREFDKILIYSKQVGYTPDYMFLLQTILRTDPQGAVNFALMMSQMEGGCPVDYNTITDLFLQRNLIREATAFLLDVLKPNLPEHAFLQTKVLEINLVTFPNVADAILANGMFSHYDRPRVAQLCEKAGLYIQSLKHYSELPDIKRVIVNTHAIEPQALVEFFGTLSSEWAMECMKDLLLVNLRGNLQIIVQACKEYCEQLGVDACIKLFEQFKSYEGLYFFLGSYLSMSEDPEIHFKYIEAAAKTGQIKEVERVTRESNFYDAEKTKNFLMEAKLPDARPLINVCDRFGFVPDLTHYLYTNNMLRYIEGYVQKVNPGNAPLVVGQLLDDECPEDFIKGLILSVRSLLPVEPLVAECEKRNRLRLLTQFLEHLVSEGSQDVHVHNALGKIIIDSNNNPEHFLTTNPYYDSKVVGKYCEKRDPTLAVVAYRRGQCDEELINVTNKNSLFKLQARYVVERMDGDLWEKVLTEENEYRRQLIDQVVSTALPESKSPEQVSAAVKAFMTADLPHELIELLEKIVLQNSAFSGNFNLQNLLILTAIKADPSRVMDYINRLDNFDGPAVGEVAVDAQLYEEAFAIFKKFNLNVQAVNVLLDNVRSIERAVEFAFRVEEDAVWSQVAKAQLREGLVSDAIESFIRADDTTQFLEVIRASEDTNVYDDLVRYLLMVRQKVKEPKVDSELIYAYAKIERLGEIEEFILMPNVANLQHVGDRLYDEALYEAAKIIYAFISNWAKLAVTLVKLQQFQGAVDAARKANSAKTWKEVCFACVDAEEFRLAQICGLNIIIQVDDLEEVSEYYQNRGCFNELISLMESGLGLERAHMGIFTELGVLYARYRYEKLMEHIKLFSTRLNIPKLIRACDEQQHWQELTYLYIQYDEFDNAATTVMNHSPEAWEHMQFKDIVAKVANVELYYKAVHFYLQEHPDIINDLLNVLALRLDHTRVVDIMRKAGHLRLIKPYMVAVQSNNVSAVNEALNEIYAEEEDYDRLRESIDLHDSFDQIGLAQKIEKHELVEMRRVAAYIYKKAGRWKQSIALSKKDNMYKDCMETASQSGDHDLAEQLLVYFIEQGKKECFATCLFVCYDLIRPDVALELAWINNMIDFAFPYLLQFIREYSGKVDELIKDKLEAQKEVKAKEQEEKDVMSQQNMYAQLLPLALPAPPMPGMGGGGYGPPPQMGGMPGMSGMPPMPPYGMPPMGGY 0850CAE77FE17616 vsr1_arath Vacuolar-sorting receptor 1 Epidermal growth factor receptor-like protein 1 BP80-like protein b Spot 3 protein VSR1 BP80B ELP ELP1 At3g52850 F8J2.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLGLFTLSFLLILNLAMGRFVVEKNNLKVTSPDSIKGIYECAIGNFGVPQYGGTLVGTVVYPKSNQKACKSYSDFDISFKSKPGRLPTFVLIDRGDCYFTLKAWIAQQAGAAAILVADSKAEPLITMDTPEEDKSDADYLQNITIPSALITKTLGDSIKSALSGGDMVNMKLDWTESVPHPDERVEYELWTNSNDECGKKCDTQIEFLKNFKGAAQILEKGGHTQFTPHYITWYCPEAFTLSKQCKSQCINHGRYCAPDPEQDFTKGYDGKDVVVQNLRQACVYRVMNDTGKPWVWWDYVTDFAIRCPMKEKKYTKECADGIIKSLGIDLKKVDKCIGDPEADVENPVLKAEQESQIGKGSRGDVTILPTLVVNNRQYRGKLEKGAVLKAMCSGFQESTEPAICLTEDLETNECLENNGGCWQDKAANITACRDTFRGRLCECPTVQGVKFVGDGYTHCKASGALHCGINNGGCWRESRGGFTYSACVDDHSKDCKCPLGFKGDGVKNCEDVDECKEKTVCQCPECKCKNTWGSYECSCSNGLLYMREHDTCIGSGKVGTTKLSWSFLWILIIGVGVAGLSGYAVYKYRIRSYMDAEIRGIMAQYMPLESQPPNTSGHHMDI 5410A089BA1E58FA ap1g1_arath AP-1 complex subunit gamma-1 At1g23900 Adaptor protein complex AP-1 large subunit gamma-1 Clathrin assembly protein complex 1 gamma-1 large chain Gamma-adaptin 1 T23E23.7 GAMMA-ADR Adaptor-related protein complex 1 subunit gamma-1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNPFSSGTRLRDMIRAIRACKTAAEERAVVRKECADIRALINEDDPHDRHRNLAKLMFIHMLGYPTHFGQMECLKLIASPGFPEKRIGYLGLMLLLDERQEVLMLVTNSLKQDLNHSNQYVVGLALCALGNICSAEMARDLAPEVERLIQFRDPNIRKKAALCSTRIIRKVPDLAENFVNAAASLLKEKHHGVLITGVQLCYELCTINDEALEYFRTKCTEGLIKTLRDITNSAYQPEYDVAGITDPFLHIRLLRLLRVLGQGDADASDLMTDILAQVATKTESNKNAGNAVLYECVETIMAIEDTNSLRVLAINILGRFLSNRDNNIRYVALNMLMKAITFDDQAVQRHRVTILECVKDPDASIRKRALELVTLLVNENNVTQLTKELIDYLEISDEDFKEDLSAKICFIVEKFSPEKLWYIDQMLKVLCEAGKFVKDDVWHALIVVISNASELHGYTVRALYKSVLTYSEQETLVRVAVWCIGEYGDLLVNNVGMLGIEDPITVTESDAVDVIEDAITRHNSDSTTKAMALVALLKLSSRFPSISERIKDIIVKQKGSLLLEMQQRAIEYNSIVDRHKNIRSSLVDRMPVLDEATFNVRRAGSFPASVSTMAKPSVSLQNGVEKLPVAPLVDLLDLDSDDIMAAPSPSGTDFLQDLLGVDLGSSSAQYGATQAPKAGTDLLLDILSIGTPSPAQNSTSSIGLLSIADVNNNPSIALDTLSSPAPPHVATTSSTGMFDLLDGLSPSPSKEATNGPAYAPIVAYESSSLKIEFTFSKTPGNLQTTNVQATFTNLSPNTFTDFIFQAAVPKFLQLHLDPASSNTLLASGSGAITQNLRVTNSQQGKKSLVMRMRIGYKLNGKDVLEEGQVSNFPRGL 3607846F5AACE546 epsin1-vt11 2 7 unspecified role unspecified role neutral component neutral component c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role neutral component neutral component c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 3Bm 3Bn 3Bo EPSIN1-positive punctate stains largely colocalised with those of VTI11:HA Q8VY07; Q9SEL6 transfection espin1-clathrin 5 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 4A 4B 4C GST:ESPIN1, but not GST alone, precipitated from plant extracts a 180kD protein species that was recognised by anti-clathrin antibody(Figure 4C), indicating that ESPIN1 bound clathrin. The identity of the protein pulled down by GST:ESPIN1 was assumed to be clathrin since , as the authors state, "GST:ESPIN1, but not GST alone, precipitated from plant extracts a 180kD protein species that was recognised by anti-clathrin antibody(Figure 4C), indicating that ESPIN1 bound clathrin." clathrin-espin1 5 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q0WNJ6:p.Leu470Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L R Wed May 02 20:15:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu470arg' Tue Jun 30 19:39:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu470Arg' ecoli Escherichia coli (strain K12) physical association physical association 4A 4B 4C GST:ESPIN1, but not GST alone, precipitated from plant extracts a 180kD protein species that was recognised by anti-clathrin antibody(Figure 4C), indicating that ESPIN1 bound clathrin. The identity of the protein pulled down by GST:ESPIN1 was assumed to be clathrin since , as the authors state, "GST:ESPIN1, but not GST alone, precipitated from plant extracts a 180kD protein species that was recognised by anti-clathrin antibody(Figure 4C), indicating that ESPIN1 bound clathrin." espin1-clathrin-1 2 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 4D Colocalisation of ESPIN1 with clathrin. Q8VY07 transfection clathrin-vsr1 3 10 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component physical association physical association S1 Clathrin was detected in a peak between 443 and 669kD(see Supplemetary Figure 1 online). Interestingly, VSR, the vacuolar cargo receptor, was eluted at the same postion. Clathrin and VSR1 eluted between 443 and 669kD. espin1-vsti11 4 8 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 5C ESPIN1 Binds to VSTI11 Q9SEL6 transformation vsti11-espin1 1 8 unspecified role unspecified role prey prey c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 5 In addition to to HA:VSTI11, ESPIN1:GFP was detected in the immunoprecipitaes. Q9SEL6; Q8VY07 transformation espin1-at1g23900 4 8 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 6A ESPIN1 Binds to gamma-ADR Q84K16 transformation at1g23900-espin1 4 11 unspecified role unspecified role bait bait n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 6B ESPIN1 Binds to gamma-ADR The authors state that the Putative gamma-adaptin , Q84K16, was expressed as the 'ear domain' of the protein tagged with the maltose binding protein epitope. The region of the ear domain was not given and could not be established. Q8VY07; Q84K16 transformation at5g22770-espin1 4 6 unspecified role unspecified role bait bait c terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 6B Interestingly, MBP:alphaADR(ear) also bound weakly to GST:ESPIN1. The authors state that the Putative alpha-adaptin , Q8LPL6, was expressed as the 'ear domain' of the protein tagged with the maltose binding protein epitope. The region of the ear domain was not given and could not be established. The authors describe the interaction between Q8LPL6 and Q8VY07 as 'weak'. Q8VY07; Q8LPL6 transformation espin1-vsr1 4 8 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 7 ESPIN1 Binds VSR1 via the ENTH Domain P93026 transformation espin1-vsr1-1 1 8 unspecified role unspecified role prey prey c terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role bait bait c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 7 In addition to VSR1:HA, EPSIN1:GFP was detected in the immunoprecipitates. P93026; Q8VY07 transformation