IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. Development (0950-1991) 2006 imex curation Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de 01-DEC-2006: Contacted by JYOTI. Returned mail. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight identificat confirmation by molecular weight weight identificat Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de Accepted 2006-NOV-20 by JYOTI. 01-DEC-2006: Contacted by JYOTI. Returned mail. Development (0950-1991) 2006 Only protein-protein interactions imex curation Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. Development (0950-1991) 2006 imex curation Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de 01-DEC-2006: Contacted by JYOTI. Returned mail. Only protein-protein interactions arath-leaf Arabidopsis thaliana leaf leaf leaf confocal microscopy confocal microscopy predetermined predetermined participant predetermined Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de Accepted 2006-NOV-20 by JYOTI. 01-DEC-2006: Contacted by JYOTI. Returned mail. Development (0950-1991) 2006 Only protein-protein interactions imex curation Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability. Development (0950-1991) 2006 imex curation Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de 01-DEC-2006: Contacted by JYOTI. Returned mail. Only protein-protein interactions arath-leaf Arabidopsis thaliana leaf leaf leaf fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Laubinger S., Marchal V., Le Gourrierec J., Gentilhomme J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U. Proteins were identified using references given in the paper. Prey proteins were fully labeled with 35Smethionine, while bait proteins were synthesized in the presence of a mixture of labeled and unlabelled methionine in order to facilitate protein detection after SDS-PAGE visualized using a phosphorimager. hoeckeru@uni-koln.de Accepted 2006-NOV-20 by JYOTI. 01-DEC-2006: Contacted by JYOTI. Returned mail. Development (0950-1991) 2006 Only protein-protein interactions imex curation spa1_arath Protein SUPPRESSOR OF PHYA-105 1 SPA1 F11C10.3/F11C10.4 At2g46340/At2g46350 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPVMERVAEETVATNNIQLKARVDDVPCNKLDARHNDMVIQSETANSDCPGSSAHRNVDLTKPPPPEEAAGAKLSVEELTLGNYRIVQGSNNTNVDSPRAGKFEHLYRLARGSAFRAGDGDLDSQPRDMDQMLSRIRQQLAGAPSERQNLKPFMSRRSDQNLEAFSERLRAAGENSIMNAPALISEGVQMKTPVSSSNFSQLLLKRAMKGKGVVGKNQETPPEFVSDQDLGSKEKKLDISKSPTPHDVLPLKSSPKGNGMVSHGDGNHSKSSIGISLREFLRSSYAKREKRHGLCLFRQLVELVDSAHSKRLFLLDLRPSLFTLVPSKKLRYIGNFGKNDLESDVDEDLNRRRPVVEESSSGGRDSKKRKMDLHLNSPGNQLQATSTGRPFKRKSPVIDLNMVDARNPDSCELQQQDYIKNLSVSSVSRKQSMSTWLEEQWYTCPEEINGEDIGEKSNIYALGVLLFELLCHCESGEMHAAMMADLRHRILPPTFLSKYPKEAGFCLWLLHPEPSSRPSARDILKSELICEDDSVKSTAAAEEISELLLHFLSSLEVQKKKKASKLLQDIQTLEDDIKEAERRYSSNVSLVRSHGAIEKRVQSSPLDEHCTTSSALFVPTANTDRLMSNIRQLEDAYFFMRSQINLSSSAATARSDKTLKDRDRCSENQNENQDMSTKGKSSDQLEVFFEGLCKFARYSKFETCGTIRSGDLLNSASVVCSLSFDPDEEHIAAAGISKKIKIFDFNAFMNESVGVHYPLVEMVNKSKLSCVCWNSYIKNYLASTDYDGVVQIWDAGTGQGFSQYTEHQKRAWSVDFSPSDPTKFVSGSDDCSVKLWSINEKRSLGTIWSPANVCCVQFSSYSNHLLAFGSADYKVYCYDLRYVKTPWCTLAGHEKAVSYVKFMDSETIVSASTDNSLKLWNLNKTNSSGLSPGACSLTYKGHTNQKNFVGLSVLDGYIACGSETNEVYSYYKSLPMPMTSYKFGSVDPISGNEYFDDNGQFVSSVCWRKKSNMLVAANSTGNMKLLKLV DIP protein Q9SYX2 original sequence version: 102 8DEDF32BB06B4A18 spa3_arath Protein SPA1-RELATED 3 SPA3 K7L4.16 At3g15354 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEGSSNSNSRGFNTSGVSDRNTEFLPVERLTTRSKPSSHVDEYVRSLFGSTSTHKSGEDDSLGIDPFVRSLEWGDVSLRQWLDKPERSVDVFECLHVFRQIVEIVNAAHSQGIVVHNVRPSCFVMSSFNHVSFIESASCSDSGSDSLEDGPISQKEIGSSRREEAVSKAIAIEEKGVYNKLLERKIEKLEEEKTQPFPMKHILAMETSWYTSPEEDFGSSSTCASDVYRLGVLLFELFCPVPSREEKSRTMSSLRHRVLPPQILLKCPKEASFCLWLLHPEPTCRPSMSDLLQSEFITEPRDNLEEREAAIELRDRIEEQESLLEFLLLIQQRKQESAYRLQDTVSLLSSDIEQVVKRQLILKKRGSSLSDFSKDDHQYTSGQPLMSFQANEEPSAFLASRKRVRQGILALENGVEVDEESQGSTLLESSRLMRNFKKLESVYFLTRRRQMKAAASGKSLTRHSPLSSENGRGSMIVSEKSSVSNPVAPKAFFNNDSRQGGWIDPFLEGLCRYLSFSQLRVKADLKQGDLLNSSNLVCALAFDREGELFATAGVNKKIKIFECNSIVNDNRDIHYPVVELAGRSKLSSLCWNSYIKSQIASSNFDGVVQIWDVARSQLVTEMKEHKKRVWSIDISSADPTLLASGSDDGTVKLWSINQGVSIGTIKTKANVCCVQFPSDSGRSLAFGSADHKVYYYDLRNPKIPLCTMIGHSKTVSYVKFVDSSTLVSSSTDNTLKLWDLSMSASGINESPLHSFTGHTNLKNFVGLSVSDGYIATGSETNEVFVYHKAFPMPVMSYMFNNTDSMSGLEVDDASQFISSICWRGQSSTLVAANSNGNIKILEMMT EB5AADEDFD16F316 spa4_arath Protein SPA1-RELATED 4 SPA4 At1g53090 F8L10.5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKGSSESSSRGLNNTSGVSEFCTDGSKSLSHIDYVRSLLGSHKEANLGGLDDDSIVRALECEDVSLRQWLDNPDRSVDAFECFHVFRQIVEIVNAAHSQGIVVHNVRPSCFVMSSFNNVSFIESASCSDSGSDEDATTKSREIGSSRQEEILSERRSKQQEEVKKQPFPMKQILAMEMSWYTSHEEDNGSLCNCASDIYRLGVLLFELFCPVSSREEKSRTMSSLRHRVLPPQILLNWPKEASFCLWLLHPEPSCRPSMSELLQSEFINEPRENLEEREAAMELRDRIEEQELLLEFLFLIQQRKQEAADKLQDTISLLSSDIDQVVKRQLVLQQKGRDVRSFLASRKRIRQGAETTAAEEENDDNSIDEESKLDDTLESTLLESSRLMRNLKKLESVYFATRYRQIKAATAAEKPLARYYSALSCNGRSSEKSSMSQPSKDPINDSRQGGWIDPFLEGLCKYLSFSKLRVKADLKQGDLLNSSNLVCAIGFDRDGEFFATAGVNKKIKIFECESIIKDGRDIHYPVVELASRSKLSGICWNSYIKSQVASSNFEGVVQVWDVARNQLVTEMKEHEKRVWSIDYSSADPTLLASGSDDGSVKLWSINQGVSIGTIKTKANICCVQFPSETGRSLAFGSADHKVYYYDLRNPKLPLCTMIGHHKTVSYVRFVDSSTLVSSSTDNTLKLWDLSMSISGINETPLHSFMGHTNVKNFVGLSVSDGYIATGSETNEVFVYHKAFPMPVLSYKFKTIDPVSELEVDDASQFISSVCWRGQSSTLVAANSTGNIKILEMV 5202D640029F512F spa2_arath Protein SPA1-RELATED 2 SPA2 F2P3.13 F2P3.14 T22B4.90 At4g11110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMDEGSVGDVSRIDEADVAHLQFKNSEQSFKPENIEVREVKEVQVQREAGSPDCSYGVIADFLDGKNGGDHVELIGNEPCSSRQNTNDEGDVVEELTVKTCEGSSMAIVGRPSSRARLEMNRSQFLHRFPLDGDLPGSSSMSKKVIDRGTVSILRNAGKMSLPETSNGQLAIIAVNGEANEHLTNVERNPVPVEALSHEGIKTKMLSQSGFSQFFVRKTLKGKGVTFRGPPNNRSKARNMDQQTVASSGSALVIANTSAKISSSIPLAAYDGLPCLPSNTSKPSSCANPSDTHRGCGGEGLSLREWLKSERQEVNKAECMYIFRQIVDHVDCSHSQGVVLCDLRPSSFKIFKENAVKYVVSGSQRESFDSNMNKETLSQLENPLVRRRLGDTSSLSIPAKKQKSSGPSSRQWPMFQRAGGVNIQTENNDGAIQEFHFRSSQPHCSTVACPFTSVSEQLEEKWYASPEELRGDMRSASSNIYSLGILLYELLSQFQCERAREAAMSDIRHRILPPKFLSENPKEAGFCLWLLHPESSCRPSTRDILQSEVVNGIPDLYAEGLSLSIEQEDTESELLQHFLFLSQEKRQKHAGNLMEEIASVEADIEEIVKRRCAIGPPSLEEASSSSPASSVPEMRLIRNINQLESAYFAARIDAHLPEARYRLRPDRDLLRNSDNTVAEVENSETWSSDDRVGAFFDGLCKYARYSKFETRGVLRTSELNNTSNVICSLGFDRDEDYFATAGVSKKIKIYEFNSLFNESVDIHYPAIEMPNRSKLSGVCWNNYIRNYLASSDYDGIVKLWDVTTGQAISHFIEHEKRAWSVDFSEACPTKLASGSDDCSVKLWNINERNCLGTIRNIANVCCVQFSPQSSHLLAFGSSDFRTYCYDLRNLRTPWCILSGHNKAVSYAKFLDNETLVTASTDNTLKLWDLKKTTHGGLSTNACSLTFGGHTNEKNFVGLSTSDGYIACGSETNEVYAYHRSLPMPITSYKFGSIDPISGKEIEEDNNLFVSSVCWRKRSNMVVSASSNGSIKVLQLV AB325211D6228E0A col1_arath Zinc finger protein CONSTANS-LIKE 1 COL1 At5g15850 F14F8_230 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLKVESNWAQACDTCRSAACTVYCRADSAYLCSSCDAQVHAANRLASRHERVRVCQSCERAPAAFFCKADAASLCTTCDSEIHSANPLARRHQRVPILPISEYSYSSTATNHSCETTVTDPENRLVLGQEEEDEDEAEAASWLLPNSGKNSGNNNGFSIGDEFLNLVDYSSSDKQFTDQSNQYQLDCNVPQRSYGEDGVVPLQIEVSKGMYQEQQNFQLSINCGSWGALRSSNGSLSHMVNVSSMDLGVVPESTTSDATVSNPRSPKAVTDQPPYPPAQMLSPRDREARVLRYREKKKMRKFEKTIRYASRKAYAEKRPRIKGRFAKKKDVDEEANQAFSTMITFDTGYGIVPSF 412C237A9CD30309 spa1-co 1 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 6B 6C The CCT domain of CO is necessary for the in vitro interaction with SPA1. The UniProt entry for CO protein has a length of 355 amino acids. However the paper describes the binding region of CO to SPA1 as being from amino acid residue 272 to residue 373. The UniProt coordinates were used to delimit the binding region of the CO protein to the SPA1 protein. spa2-co 1 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 5A SPA proteins interact with CO in vitro. spa3-co 1 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 5A SPA proteins interact with CO in vitro. spa4-co 1 6 unspecified role unspecified role bait bait gad tag GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 5A SPA proteins interact with CO in vitro. spa1-co-1 3 4 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 5C 5D FRET analysis by photoacceptor bleaching further confirms that SPA1 and CO interact in planta. spa1-co-2 2 8 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 5B Co-localization of CFP-SPA1 and YFP-CO in transiently transfected Arabidopsis leaf The proteins colocalised to the nucleus.