UniProt UniProt http://www.uniprot.org The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. Mol. Cell (1097-2765) 2006 imex curation VanDemark AP., Blanksma M., Ferris E., Heroux A., Hill CP., Formosa T. Purified Replication factor-A (RPA) and SPT16-POB3 have been mixed and probed with antisera. chris@biochem.utah.edu 26-JUL-2004: Contacted by JYOTI Only protein-protein interactions in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot VanDemark AP., Blanksma M., Ferris E., Heroux A., Hill CP., Formosa T. Purified Replication factor-A (RPA) and SPT16-POB3 have been mixed and probed with antisera. chris@biochem.utah.edu Accepted 2006-JUL-14 by ORCHARD. 26-JUL-2004: Contacted by JYOTI Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition. Mol. Cell (1097-2765) 2006 imex curation VanDemark AP., Blanksma M., Ferris E., Heroux A., Hill CP., Formosa T. Purified Replication factor-A (RPA) and SPT16-POB3 have been mixed and probed with antisera. chris@biochem.utah.edu 26-JUL-2004: Contacted by JYOTI Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot VanDemark AP., Blanksma M., Ferris E., Heroux A., Hill CP., Formosa T. Purified Replication factor-A (RPA) and SPT16-POB3 have been mixed and probed with antisera. chris@biochem.utah.edu Accepted 2006-JUL-14 by ORCHARD. 26-JUL-2004: Contacted by JYOTI Mol. Cell (1097-2765) 2006 Only protein-protein interactions imex curation pob3_yeast FACT complex subunit POB3 YML069W Facilitates chromatin transcription complex subunit POB3 POB3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTDFDRIYLNQSKFSGRFRIADSGLGWKISTSGGSAANQARKPFLLPATELSTVQWSRGCRGYDLKINTKNQGVIQLDGFSQDDYNLIKNDFHRRFNIQVEQREHSLRGWNWGKTDLARNEMVFALNGKPTFEIPYARINNTNLTSKNEVGIEFNIQDEEYQPAGDELVEMRFYIPGVIQTNVDENMTKKEESSNEVVPKKEDGAEGEDVQMAVEEKSMAEAFYEELKEKADIGEVAGDAIVSFQDVFFTTPRGRYDIDIYKNSIRLRGKTYEYKLQHRQIQRIVSLPKADDIHHLLVLAIEPPLRQGQTTYPFLVLQFQKDEETEVQLNLEDEDYEENYKDKLKKQYDAKTHIVLSHVLKGLTDRRVIVPGEYKSKYDQCAVSCSFKANEGYLYPLDNAFFFLTKPTLYIPFSDVSMVNISRAGQTSTSSRTFDLEVVLRSNRGSTTFANISKEEQQLLEQFLKSKNLRVKNEDREVQERLQTALGSDSDEEDINMGSAGEDDESVDEDFQVSSDNDADEVAEEFDSDAALSDAEGGSDEERPSKKPKVE 02C04F620C7B1450 02C04F620C7B1450 rfa1_yeast Replication factor A protein 1 RFA1 BUF2 YAR007C FUN3 Replication protein A 69 kDa DNA-binding subunit Single-stranded DNA-binding protein DNA-binding protein BUF2 RPA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSVQLSRGDFHSIFTNKQRYDNPTGGVYQVYNTRKSDGANSNRKNLIMISDGIYHMKALLRNQAASKFQSMELQRGDIIRVIIAEPAIVRERKKYVLLVDDFELVQSRADMVNQTSTFLDNYFSEHPNETLKDEDITDSGNVANQTNASNAGVPDMLHSNSNLNANERKFANENPNSQKTRPIFAIEQLSPYQNVWTIKARVSYKGEIKTWHNQRGDGKLFNVNFLDTSGEIRATAFNDFATKFNEILQEGKVYYVSKAKLQPAKPQFTNLTHPYELNLDRDTVIEECFDESNVPKTHFNFIKLDAIQNQEVNSNVDVLGIIQTINPHFELTSRAGKKFDRRDITIVDDSGFSISVGLWNQQALDFNLPEGSVAAIKGVRVTDFGGKSLSMGFSSTLIPNPEIPEAYALKGWYDSKGRNANFITLKQEPGMGGQSAASLTKFIAQRITIARAQAENLGRSEKGDFFSVKAAISFLKVDNFAYPACSNENCNKKVLEQPDGTWRCEKCDTNNARPNWRYILTISIIDETNQLWLTLFDDQAKQLLGVDANTLMSLKEEDPNEFTKITQSIQMNEYDFRIRAREDTYNDQSRIRYTVANLHSLNYRAEADYLADELSKALLA 7EB8DFA6910EF8A8 7EB8DFA6910EF8A8 pob3-rfa1-1 1 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 5B rfa1-pob3-rfa1-1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 5B pob3-rfa1-2 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q04636:p.Gln308Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Q R Wed May 02 19:34:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln308arg' Tue Jun 30 18:13:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln308Arg' Q04636:p.Gln308Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Q K Wed May 02 20:29:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln308lys' Tue Jun 30 20:16:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln308Lys' physical association physical association 5C Histidine-tagged versions of POB3 used to show interaction: POB3 fragment aa237-477, SPT16-POB3 with or without indicated POB3 mutations and SPT16-POB3(phe133ser).