MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it SUMO conjugation attenuates the activity of the gypsy chromatin insulator. SUMO conjugation attenuates the activity of the gypsy chromatin insulator. EMBO J. (0261-4189) 2006 imex curation Capelson M., Corces VG. 2009-01-08 Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Capelson M., Corces VG. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation SUMO conjugation attenuates the activity of the gypsy chromatin insulator. SUMO conjugation attenuates the activity of the gypsy chromatin insulator. EMBO J. (0261-4189) 2006 imex curation Capelson M., Corces VG. 2009-01-08 Only protein-protein interactions drome-larval _immaginal_disc Drosophila melanogaster larva_ imaginal_disc larval imaginal disc fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Capelson M., Corces VG. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation o97102_drome Small ubiquitin-related modifier smt3 Smt3 DmSUMO-1 Dm0342 DmSmt3 Dmel\CG4494 Dmsmt3 SMT3 SUMO Sumo anon-EST:Posey240 dSmt3 dsmt3 l(2)04493 l(2)SH0182 l(2)SH2 0182 CG4494 sumo Dmel_CG4494 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSDEKKGGETEHINLKVLGQDNAVVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGAP E49DA04C24A13CA5 mmd4_drome Modifier of mdg4 mod(mdg4) bpd doom E(var)3-93D CG32491 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MADDEQFSLCWNNFNTNLSAGFHESLCRGDLVDVSLAAEGQIVKAHRLVLSVCSPFFRKMFTQMPSNTHAIVFLNNVSHSALKDLIQFMYCGEVNVKQDALPAFISTAESLQIKGLTDNDPAPQPPQESSPPPAAPHVQQQQIPAQRVQRQQPRASARYKIETVDDGLGDEKQSTTQIVIQTTAAPQATIVQQQQPQQAAQQIQSQQLQTGTTTTATLVSTNKRSAQRSSLTPASSSAGVKRSKTSTSANVMDPLDSTTETGATTTAQLVPQQITVQTSVVSAAEAKLHQQSPQQVRQEEAEYIDLPMELPTKSEPDYSEDHGDAAGDAEGTYVEDDTYGDMRYDDSYFTENEDAGNQTAANTSGGGVTATTSKAVVKQQSQNYSESSFVDTSGDQGNTEAQAATSASATKIPPRKRGRPKTKVEDQTPKPKLLEKLQAATLNEEASEPAVYASTTKGGVKLIFNGHLFKFSFRKADYSVFQCCYREHGEECKVRVVCDQKRVFPYEGEHVHFMQASDKSCLPSQFMPGESGVISSLSPSKELLMKNTTKLEEADDKEDEDFEEFEIQEIDEIELDEPEKTPAKEEEVDPNDFREKIKRRLQKALQNKKK 79CC78E52D2ADAF5 cp190_drome Centrosome-associated zinc finger protein CP190 Cp190 E(mod)4-1 CG6384 dMAP190 Protein enhancer of mod(mdg4)4-1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGEVKSVKVDNWGVFFLQKLQNFFNKTDYCDLTLQFRDNSQLKVHRLVLSACTDYFNVLEQTCEIVDDALIMPNEFQADVVVPIVNFMYTGTLEFELKMYGKLLRTAKEMNMTVLLKLLEAHRRTMENVNRQQRPPSPKGIRRRTVGQPSSGLPQQRVLGPSPQSRNVATPIAQRANTQRGSTGNTMSRTSGGSNRSPYGDSSNVKQEPTSPFEQLRKGYNNNKRPAQTSLLSPPSKKPSLEEVKEFAEQQRMRKQIAAEYGDNDPEYDGGMLYDDVHAGDDDDDDMPPQPSTSKQQSPQGTQTQLEHGSTTIILKQDSPSQTPTIIVKDSSNAKLNHTKIIAEVLRQYPHIVKGHKNIKLKIMPNTPAAPTEKSAPATVKPPANQSSATTSPHKKLHVSFKADKSTPLITAQQKAASSQQKSGTSQTTGNQGTGANPPANTAAAQKRRIDSKTMHALIAQGAENTTGPWLCLRCGVNGRPISIPSYRGFRRHLINTHKETIDPALCEHCGWRSVNNRELHFHMYMEHQTKSLLYTFAECALCNQSYRTKGELEAHINEVHTDDNKQQCIYCNKVFEQELQLYRHMKSYHKEQALEDGIIDETDEEFLGSQDEEEEAEGDEEQEPEQTGKVRILSDISLPATSAITVQQAQQEQLQEEDVEQVQQEVKFVGADGNEVELTDEQRKEILSQLNQQQAGATAGGVVMVLSEPEAEHVKQETDEKSLAGTEEEYDDSQIYSELGAADSVESAKKNIADESKESIDNLEWAENLIAESEEQSNKEPKSDKPRDDISEKLKELTGDWTEDENDDDVDDKPATAELASELANKDPEPTVHEEEDDIDLALQSLHKGPEEATEEKASEESVTSADDAVDAVPNINSQPEKMDVDSEAADEKASKAEVQIKKEAELENDQEEFIKEDSPIPHSDSVAELREAVTASEGEDDVHLEADNIRKELLDELIAEAEKPDQEKDIVQSEENATTEALDRSVTDEDDLVPPTQVSTEQMEIDEPAAEKAAENNEDTRTADEKEAVEDKPNQTQDVTTAEKPTLESAKAGDEATSGEAASVDKVKSLISEWGDDDEDEDENGVSAAAKEEL 5CD5C5492B948D39 q7knm2_drome lwr semi Ubc9 DUbc9 DmUbc9 Dmel\CG3018 Dmubc9 FBgn0010602 Iwr Lwr Ubc 9 Ubc-9 UbcD9 dUBC9 dUbc9 dip4 hbl i105 i56 l(2)01519 l(2)02858 l(2)05486 l(2)05487 ubc9 Dmel_CG3018 UBC9 Ubc9 CG3018 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGIAITRLGEERKAWRKDHPFGFVARPAKNPDGTLNLMIWECAIPGKKSTPWEGGLYKLRMIFKDDYPTSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNIKDPAQAEAYTIYCQNRLEYEKRVRAQARAMAATE 86FE1870845CFEE3 mod_mdg4_-smt3 2 3 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell Stoichiometry: 1.0 4 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell Stoichiometry: 1.0 colocalization colocalization F3A EMBO-Journal A fraction of Mod(mdg4)2.2 sites, which are found exclusively at the border regions, colocalize with SUMO, as revealed by costaining of polytene chromosomes with anti-SUMO and anti-Mod(mdg4)2.2 antisera (Figure 3A) mint cp190-smt3 2 5 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell Stoichiometry: 1.0 3 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell Stoichiometry: 1.0 colocalization colocalization F3B EMBO-Journal Similarly, SUMO is detected at a subset of CP190 chromatin binding sites homomint mint lwr-mod_mdg4_ 1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 direct interaction direct interaction F1D EMBO-Journal We confirmed this association for Mod(mdg4)2.2 and Ubc9 using a GST pull-down assay (Figure 1D, left panel). mint