MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR. PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR. EMBO J. (0261-4189) 2006 imex curation Nagano K., Bornhauser BC., Warnasuriya G., Entwistle A., Cramer R., Lindholm D., Naaby-Hansen S. 2009-01-08 Only protein-protein interactions mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nagano K., Bornhauser BC., Warnasuriya G., Entwistle A., Cramer R., Lindholm D., Naaby-Hansen S. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR. PDGF regulates the actin cytoskeleton through hnRNP-K-mediated activation of the ubiquitin E3-ligase MIR. EMBO J. (0261-4189) 2006 imex curation Nagano K., Bornhauser BC., Warnasuriya G., Entwistle A., Cramer R., Lindholm D., Naaby-Hansen S. 2009-01-08 Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nagano K., Bornhauser BC., Warnasuriya G., Entwistle A., Cramer R., Lindholm D., Naaby-Hansen S. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 mylip_mouse E3 ubiquitin-protein ligase MYLIP Mylip Inducible degrader of the LDL-receptor Myosin regulatory light chain-interacting protein RING-type E3 ubiquitin transferase MYLIP protein protein mouse Mus musculus Mouse MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKESLLAGHLQCSPEQAVELSALLAQTKFGDYNQNTAQYSYEDLCEKELSSSTLNSIVAKHKELEGISQASAEYQVLQIVSAMENYGIEWHAVRDSEGQKLLIGVGPEGISICKEDFSPINRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAGVVDLVSRSDQSPPSSPLKSSDSSMSCSSCEGLSCQQTRVLQEKLRKLKEAMLCMACCEEEINSTFCPCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI mint E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Stable or transient overexpression of MIR induces proteasomal degradation of MRLC inhibiting neurite outgrowth in presence of NGF. MIR counteracts the stabilization of MRLC by MIR-interacting saposin-like protein (MSAP/TMEM4) and reduces MSAP-stimulated neurite outgrowth 31C73572C95D0711 ml12b_mouse Myosin regulatory light chain 12B Mrlc2 Myosin regulatory light chain 2-B, smooth muscle isoform Myl12b Mylc2b Myosin regulatory light chain MRLC2 Myosin regulatory light chain 20 kDa protein protein mouse Mus musculus Mouse MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD DIP protein Q3THE2 original sequence version: 71 78FF911630F3870B myl12b-rps27a 2 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F3B F3C EMBO-Journal Immunostaining of immunoprecipitated (IP) MRLC with antibody against ubiquitin confirmed that PDGF induced ubiquitination of MRLC (Figure 3B and C). mint mylip-myl12b 1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F4C EMBO-Journal Intriguingly, MRLC phosphorylated at threonine 18 and serine 19 copurified with MIR in immunoprecipitates from PDGFstimulated, but not from -untreated cells (Figure 4C). homomint mint