MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p. Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p. EMBO J. (0261-4189) 2006 imex curation Stroupe C., Collins KM., Fratti RA., Wickner W. 2009-01-08 Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom predetermined predetermined participant predetermined Stroupe C., Collins KM., Fratti RA., Wickner W. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p. Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p. EMBO J. (0261-4189) 2006 imex curation Stroupe C., Collins KM., Fratti RA., Wickner W. 2009-01-08 Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Stroupe C., Collins KM., Fratti RA., Wickner W. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation vps41_yeast Vacuolar protein sorting-associated protein 41 VPS41 FET2 Vacuolar morphogenesis protein 2 VAM2 D446 YD8554.13 YDR080W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTDNHQNDSVLDQQSGERTIDESNSISDENNVDNKREDVNVTSPTKSVSCISQAENGVASRTDESTITGSATDAETGDDDDDDDDDDDEDEDDEDEPPLLKYTRISQLPKNFFQRDSISSCLFGDTFFAFGTHSGILHLTTCAFEPIKTIKCHRSSILCINTDGKYFATGSIDGTVIIGSMDDPQNITQYDFKRPINSVALHSNFQASRMFVSGGMAGDVVLSQRNWLGNRIDIVLNKKKKKKTRKDDLSSDMKGPIMGIYTMGDLILWMDDDGITFCDVPTRSQLLNIPFPSRIFNVQDVRPDLFRPHVHFLESDRVVIGWGSNIWLFKVSFTKDSNSIKSGDSNSQSNNMSHFNPTTNIGSLLSSAASSFRGTPDKKVELECHFTVSMLITGLASFKDDQLLCLGFDIDIEEEATIDEDMKEGKNFSKRPENLLAKGNAPELKIVDLFNGDEIYNDEVIMKNYEKLSINDYHLGKHIDKTTPEYYLISSNDAIRVQELSLKDHFDWFMERKQYYKAWKIGKYVIGSEERFSIGLKFLNSLVTKKDWGTLVDHLNIIFEETLNSLDSNSYDVTQNVLKEWADIIEILITSGNIVEIAPLIPKKPALRKSVYDDVLHYFLANDMINKFHEYITKWDLKLFSVEDFEEELETRIEAASEPTASSKEEGSNITYRTELVHLYLKENKYTKAIPHLLKAKDLRALTIIKIQNLLPQYLDQIVDIILLPYKGEISHISKLSIFEIQTIFNKPIDLLFENRHTISVARIYEIFEHDCPKSFKKILFCYLIKFLDTDDSFMISPYENQLIELYSEYDRQSLLPFLQKHNNYNVESAIEVCSSKLGLYNELIYLWGKIGETKKALSLIIDELKNPQLAIDFVKNWGDSELWEFMINYSLDKPNFTKAILTCSDETSEIYLKVIRGMSDDLQIDNLQDIIKHIVQENSLSLEVRDNILVIINDETKKFANEFLKIRSQGKLFQVDESDIEINDDLNGVL E1E48B4D5A1A4005 E1E48B4D5A1A4005 vps33_yeast Vacuolar protein sorting-associated protein 33 VPS33 SLP1 Protein SLP1 Vacuolar morphogenesis protein 5 VAM5 L8084.15 YLR396C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNRFWNTKKFSLTNADGLCATLNEISQNDEVLVVQPSVLPVLNSLLTFQDLTQSTPVRKITLLDDQLSDDLPSALGSVPQMDLIFLIDVRTSLRLPPQLLDAAQKHNLSSLHIIYCRWKPSFQNTLEDTEQWQKDGFDLNSKKTHFPNVIESQLKELSNEYTLYPWDLLPFPQIDENVLLTHSLYNMENVNMYYPNLRSLQSATESILVDDMVNSLQSLIFETNSIITNVVSIGNLSKRCSHLLKKRIDEHQTENDLFIKGTLYGERTNCGLEMDLIILERNTDPITPLLTQLTYAGILDDLYEFNSGIKIKEKDMNFNYKEDKIWNDLKFLNFGSIGPQLNKLAKELQTQYDTRHKAESVHEIKEFVDSLGSLQQRQAFLKNHTTLSSDVLKVVETEEYGSFNKILELELEILMGNTLNNDIEDIILELQYQYEVDQKKILRLICLLSLCKNSLREKDYEYLRTFMIDSWGIEKCFQLESLAELGFFTSKTGKTDLHITTSKSTRLQKEYRYISQWFNTVPIEDEHAADKITNENDDFSEATFAYSGVVPLTMRLVQMLYDRSILFHNYSSQQPFILSREPRVSQTEDLIEQLYGDSHAIEESIWVPGTITKKINASIKSNNRRSIDGSNGTFHAAEDIALVVFLGGVTMGEIAIMKHLQKILGKKGINKRFIIIADGLINGTRIMNSIS ED78DF55109C20E7 ED78DF55109C20E7 vam6_yeast Vacuolar morphogenesis protein 6 Vacuolar protein sorting-associated protein 39 CVT4 VAM6 VPL18 VPL22 VPS39 YDL077C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRAQKLHSLKSSDITAILPTEQSQKLVLAKKNGDVEVYSRDGNTLKLFQVYPDLLQNAKNDPLPPVIENFYFANELSTIFAQCKETLILLSTTNLHEYDRIIDRRGINHCWLFERSHKNKEEKNTYLIYSTINTAKMRVLIWEGRTYKNMMEASLSYRKETIRSIYPGETGITLATDLGIYHWPYNKPSLIRIEKTVKNKFPKDMISALTELKEQAEKVIEKKPKKNSHFDAQSFSSMDRMSRKSSMSSLWYRTIRNERGNKIRYTFELDGNDATPMIIDGATKKIFKVELMHNNEEPFLIATDHATFSESNSEFDHMQYLSSNLLMLYNSSTIKFVDYENGFTFLQQKIPEGIKWVKNLSGTYFLVWTSNDEVQLFSYHVDDGSEDDDQESICGDINDPDFYQLWRKVLFYKFFIDSPHSKELCVSDNPEESLDICAMKLRDLTVMWCLRIFDKFQNYMVQLERSRNSRMIRSKCEEMIIKSIFDLFIKFWAPPQLVILKVFPSAISSLVLEITGQEHHCLLKEAEEVKETYDIPPHLLNRWCLPYLTDTRRHLQNLLSKENDDESRITWCYRDREIKQSFDFFLISNHDDVDLNTMLTLIDTVLFKCYLYYNPPMVGPFIRVENHCDSHVIVTELKIRHMFKDLIDFYYKRGNHEEALKFLTDLVDELENDNTDQKQRQKIDHGVKILVIYYLKKLSNPQLDVIFTYTDWLLNRHNDSIKEILSSIFFYDSQACSSRDHLKVYGYIKKFDKLLAIQYLEFAISTFRLEGNKLHTVLIKLYLENLDIPSTRIKLKSLLETTSVYEPRTILKLLNDAIESGSDQLPTNQLNFVKYLKIFPLSKLENHKEAVHILLDEIDDYKAATSYCNDVYQSDSTKGEELLLYLYSKLVSIYDSNRNSKLILNFLQDHGSKLNSAEIYKNLPQDISLYDIGRVVSQLLKKHTSKMDETRLEKALLQVELVATTYKLNERMSSYGVLSDSHKCPICKKVISNFGTDSISWFTREGRNIITHYNCGKVLQERFNAKNEKSSRIKQKTLGEVINELNNK EE8D94E7E2F5F078 EE8D94E7E2F5F078 pep3_yeast Vacuolar membrane protein PEP3 VAM8 VPS18 VPT18 L9634.2 YLR148W PEP3 Vacuolar protein sorting-associated protein 18 Vacuolar morphogenesis protein 8 Vacuolar protein-targeting protein 18 Carboxypeptidase Y-deficient protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MIKTRIEEVQLQFLTGNTELTHLKVSNDQLIVTTQRTIYRINLQDPAIVNHFDCPLSKELETIMNVHVSPMGSVILIRTNFGRYMLLKDGEFTQLNKIKNLDLSSLHWINETTFLMGIKKTPKLYRVELTGKDITTKLWYENKKLSGGIDGIAYWEGSLLLTIKDNILYWRDVTNMKFPLVLPDESEQFERLKHHAIKKFDSYNGLFAWVTSNGIVFGDLKEKQMEKDPASNNFGKFLSSSKVLLNFELPDYQNDKDHLIKDIVLTAFHILLLRKNTVTMVSQLNNDVVFHETIPRHQLTGSNTDSNEKFLGLVRDSVKETFWCFSNINVFEIIIENEPNSVWNLLVRDNKFDKALSLKGLTVREIESVKLSKAMYLFHTAKDFHSAAQTLGSMKDLSHFGEIALNFLQIKDYNDLNVILIKQLDNVPWKSTQVVLSSWIIWNFMKQLNDIELKINTTKPASTDEDNLLNWNLNLKEKSNELTKFLESHLEKLDNETVYQIMSKQNRQNELLIFASLINDMKFLLSFWIDQGNWYESLKILLTINNHDLVYKYSLILLLNSPEATVSTWMKIKDLDPNKLIPTILKFFTNWQNNSKLITNISEYPENYSLTYLKWCVREVPKMCNPIVYNSILYMMITDPRNDMILENDIIKFMKSNENKYDLNFQLRLSLKFKKTKTSIFLLTRLNLFEDAIDLALKNNLIDDCKVIVNDEILIEDYKLRKRLWLKIAKHLLLSMKDIDIKQLIRTILNDSNEILTIKDLLPFFNEYTTIANLKEELIKFLENHNMKMNEISEDIINSKNLKVEINTEISKFNEIYRILEPGKSCDECGKFLQIKKFIVFPCGHCFHWNCIIRVILNSNDYNLRQKTENFLKAKSKHNLNDLENIIVEKCGLCSDININKIDQPISIDETELAKWNE 37AE3C34AE0470B8 37AE3C34AE0470B8 vam7_yeast Vacuolar morphogenesis protein 7 VAM7 YGL212W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSEFWKLKTRLERDVGSTIPYDFPEKPGVLDRRWQRRYDDPEMIDERRIGLERFLNELYNDRFDSRWRDTKIAQDFLQLSKPNVSQEKSQQHLETADEVGWDEMIRDIKLDLDKESDGTPSVRGALRARTKLHKLRERLEQDVQKKSLPSTEVTRRAALLRSLLKECDDIGTANIAQDRGRLLGVATSDNSSTTEVQGRTNNDLQQGQMQMVRDQEQELVALHRIIQAQRGLALEMNEELQTQNELLTALEDDVDNTGRRLQIANKKARHFNNSA 2F992AEC0ACBC8FE 2F992AEC0ACBC8FE vps16_yeast Vacuolar protein sorting-associated protein 16 VPS16 VAM9 VPT16 YPL045W Vacuolar morphogenesis protein 9 Vacuolar protein-targeting protein 16 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKNPSFDWERLKDVFYRSRAIGELKWPTQYEEFKCALSLTVIAVEIQDFIQVYNYFGQLLGKINLQRIHEDIIKFEFDKDEKLILVTKSSIKIVKGWSPLTIESVPLQDPTIDTIWDYHNGIMLLAKSRDIYKLNGNEWELLYENKDKKYNLLTKNHWSCNDDSIILLDVDHVYQVSTSNGALLKLITDSSWHKVTISSRGFICLYNMKDNKLQIFRDPARILMEHNLDSTPDDICWCGNDTVACSFEDEIKLYGPDGLYVTFWYPFTVTNLRAEVDGLKVITTEKIYFLSRVQPQTSNIFRIGSTEPGAMLVDSFSLLEDHAPKAIEILKNFVLEKGVLDCIAAAIDEFEPKLQKMLLNAASYGKASLQYKSFDASIFVNACNTIKLLNCFRSFGIFLTVEEYRCISLKGVIDRLLKYHRYYECIQICKLANERFLLGYVFTEWAKDKIKGSPDMEDDELLDKIKSRLSVIDMTDTLQMVAVAKVAYLEGRFQLSRNLALLEKNEEARIEQLYNLDDDSIALKECIKVQNYSLTISLLIALSKKLTNSQLTKLLIIDMFNNPLYLYYMRMDKAYLYDFYRQTDRFIDLAHVLLQQGKEQQSLHSFLPQIKDLYSQVQNSEVVNNTIEQLQRQEKLWIYQESLGKRFAISFTNMTLDQTLSKLIETGQDKQVKEIVKKFKISEKKLYHLKCKTLVEAKKFDELLQFAQSRKSPIGYMPFYTYLKSRGHMDKASPYVNMIPGLSYQEKKKLYVECRGFRDAIQLAGKEKDIPGLKEIYNIIPPNEPELKALANETMSRI 6AE1ED43DB234137 pep5_yeast E3 ubiquitin-protein ligase PEP5 PEP5 END1 Histone E3 ligase PEP5 Vacuolar biogenesis protein END1 Vacuolar protein sorting-associated protein 11 Vacuolar morphogenesis protein 1 Vacuolar protein-targeting protein 11 Carboxypeptidase Y-deficient protein 5 VAM1 VPL9 VPS11 VPT11 YM9959.13 YMR231W RING-type E3 ubiquitin transferase PEP5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLSSWRQFQLFENIPIRDPNFGGDSLLYSDPTLCAATIVDPQTLIIAVNSNIIKVVKLNQSQVIHEFQSFPHDFQITFLKVINGEFLVALAESIGKPSLIRVYKLEKLPNREQLYHSQVELKNGNNTYPISVVSISNDLSCIVVGFINGKIILIRGDISRDRGSQQRIIYEDPSKEPITALFLNNDATACFAATTSRILLFNTTGRNRGRPSLVLNSKNGLDLNCGSFNPATNEFICCLSNFIEFFSSSGKKHQFAFDLSLRKRIFCVDKDHILIVTEETGVPTTSISVNELSPTIINRIFIIDAKNKIISLNFVVSSAIIDIFSTSQSGKNITYLLTSEGVMHRITPKSLENQINIIIQKELYPFALQLAKQHSLSPLDVQEIHKKYGDYLFKKGLRKEATDQYIQCLDVVETSEIISKFGVKEVPDPESMRNLADYLWSLIKNSISQRDHVTLLLIVLIKLKDVEGIDTFIQHFDRKGIWNEGVVMDDMDDVTFFYSDNDFFDLDLILELMKESDFKRLSYRLAKKYSKDSLIIVDILLNLLHNPVKAIKYIKSLPIDETLRCLVTYSKKLLEESPNETNALLIEVFTGKFKPSTFEVDLDRRDTTGDFSENIRTVFYSYKTFFNYMNSNGTSDAMSESSEASHEHEEPTYHPPKPSIVFSSFVTKPFEFVVFLEACLACYQQYEGFDEDRQVILTTLYDLYLNLAQNDVPERIDDWRSRATGVLRESNKLVYSAASNNTSKRVDNSIMLLISHMDQSSASAKDKTKIDIASFANDNPEMDLLSTFRAMTLNEEPSTCLKFLEKYGTEEPKLLQVALSYFVSNKLIFKEMGGNEVLKEKVLRPIIEGERMPLLDIIKALSRTNVAHFGLIQDIIIDHVKTEDTEIKRNEKLIESYDKELKEKNKKLKNTINSDQPLHVPLKNQTCFMCRLTLDIPVVFFKCGHIYHQHCLNEEEDTLESERKLFKCPKCLVDLETSNKLFEAQHEVVEKNDLLNFALNSEEGSRDRFKVITEFLGRGAISYSDITI E5216AD3C285CF34 E5216AD3C285CF34 vps33-pep5 1 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tagged molecule tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 9 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F1B EMBO-Journal Purified vacuoles bearing Vps33-SBP were lysed in detergent and subjected to streptavidin affinity chromatography. Only the other five known HOPS complex subunits co-purified at high levels with SBP-tagged Vps33 (Figure 1). mint vps33-pep3 1 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tagged molecule tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F1B EMBO-Journal Purified vacuoles bearing Vps33-SBP were lysed in detergent and subjected to streptavidin affinity chromatography. Only the other five known HOPS complex subunits co-purified at high levels with SBP-tagged Vps33 (Figure 1). homomint mint vps33-vps16 1 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tagged molecule tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 8 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F1B EMBO-Journal Purified vacuoles bearing Vps33-SBP were lysed in detergent and subjected to streptavidin affinity chromatography. Only the other five known HOPS complex subunits co-purified at high levels with SBP-tagged Vps33 (Figure 1). homomint mint vps33-vps41 1 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tagged molecule tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F1B EMBO-Journal Purified vacuoles bearing Vps33-SBP were lysed in detergent and subjected to streptavidin affinity chromatography. Only the other five known HOPS complex subunits co-purified at high levels with SBP-tagged Vps33 (Figure 1). homomint mint vps33-vam6 1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 4 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed tagged molecule tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F1B EMBO-Journal Purified vacuoles bearing Vps33-SBP were lysed in detergent and subjected to streptavidin affinity chromatography. Only the other five known HOPS complex subunits co-purified at high levels with SBP-tagged Vps33 (Figure 1). homomint mint vam7-vam6 2 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain range MINT-1898171: original sequence: 1-124 : MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSEFWKLKTRLERDVGSTIPYDFPEKPGVLDRRWQRRYDDPEMIDERRIGLERFLNELYNDRFDSRWRDTKIAQDFLQLSKP gst tagged gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F7 EMBO-Journal HOPS complex from vacuole lysates showed the strongest interaction with GST-Vam7p (Figure 7A). mint vam7-vps41 2 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain range MINT-1898188: original sequence: 1-124 : MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSEFWKLKTRLERDVGSTIPYDFPEKPGVLDRRWQRRYDDPEMIDERRIGLERFLNELYNDRFDSRWRDTKIAQDFLQLSKP gst tagged gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F7 EMBO-Journal HOPS complex from vacuole lysates showed the strongest interaction with GST-Vam7p (Figure 7A). mint vam7-vps33 2 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain range MINT-1898213: original sequence: 1-124 : MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSEFWKLKTRLERDVGSTIPYDFPEKPGVLDRRWQRRYDDPEMIDERRIGLERFLNELYNDRFDSRWRDTKIAQDFLQLSKP gst tagged gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 physical association physical association F7 EMBO-Journal HOPS complex from vacuole lysates showed the strongest interaction with GST-Vam7p (Figure 7A). mint vam7-pep3 2 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain range MINT-1898226: original sequence: 1-124 : MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSEFWKLKTRLERDVGSTIPYDFPEKPGVLDRRWQRRYDDPEMIDERRIGLERFLNELYNDRFDSRWRDTKIAQDFLQLSKP gst tagged gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F7 EMBO-Journal HOPS complex from vacuole lysates showed the strongest interaction with GST-Vam7p (Figure 7A). mint