MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Identification and characterization of a G-protein regulatory motif in WAVE1. Identification and characterization of a G-protein regulatory motif in WAVE1. FEBS Lett. (0014-5793) 2006 imex curation Song KS., Peterson YK., Freidman A., Blumer JB., Sato M., Lanier SM. 2009-01-08 Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Song KS., Peterson YK., Freidman A., Blumer JB., Sato M., Lanier SM. FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation Identification and characterization of a G-protein regulatory motif in WAVE1. Identification and characterization of a G-protein regulatory motif in WAVE1. FEBS Lett. (0014-5793) 2006 imex curation Song KS., Peterson YK., Freidman A., Blumer JB., Sato M., Lanier SM. 2009-01-08 Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Song KS., Peterson YK., Freidman A., Blumer JB., Sato M., Lanier SM. FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation arpc2_human Actin-related protein 2/3 complex subunit 2 ARPC2 ARC34 PRO2446 Arp2/3 complex 34 kDa subunit protein protein human Homo sapiens Human MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR DIP protein O15144 original sequence version: 99 DIP protein O15144 original sequence version: 122 DIP protein O15144 original sequence version: 119 DIP protein O15144 original sequence version: 140 3BA57121BE9A05F2 gnai3_rat Guanine nucleotide-binding protein G(i) subunit alpha-3 G(i) alpha-3 Gnai3 Gnai-3 protein protein rat Rattus norvegicus (Rat) Rat MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTSELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQTNYIPTQQDVLRTRVKTTGIVETHFTFKELYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEVYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY A65D63CE5A32C777 gbb1_rat Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Transducin beta chain 1 Gnb1 protein protein rat Rattus norvegicus (Rat) Rat MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN DIP protein P54311 original sequence version: 123 DIP protein P54311 original sequence version: 96 896CBD32D2686598 wasf1_rat Wiskott-Aldrich syndrome protein family member 1 Wasf1 Wave1 Protein WAVE-1 protein protein rat Rattus norvegicus (Rat) Rat MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNVLTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDADLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLSRAEERVLVRPHEPPPPPPMHAAGDARPTPTCVSSAAGLIENRPQSPAAGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPAPALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVAALAHPPSGLHPTPSPAPGPHAPLMPPSPPSQVLPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE DIP protein Q5BJU7 original sequence version: 54 mint Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton 5682117C6281D401 actb_rat Actin, cytoplasmic 1 Actb Beta-actin protein protein rat Rattus norvegicus (Rat) Rat MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 6AFD05CA94E360E2 wasf1-gnai3 2 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F3A The interaction is regulated by the presence of GTPγS. Endogenous Giα3 and WAVE1 were coimmunoprecipitated from rat brain lysates and this interaction was disrupted by activation of G-proteins with GTPγS (see Fig. 3A), indicating that the interaction is regulated. FEBS-Letters mint wasf1-actb 2 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F3A The interaction is regulated by the presence of GTPγS. F3: Rat brain lysates (2 mg) (A) were preincubated with 100 μM GDP or 100 μM GTPγS and 25 mM MgCl2 at 24 °C for 30 min prior to immunoprecipitation. Membrane transfers were first blotted with Giα3 antisera (1:5000) and then stripped and sequentially reprobed with antibodies or sera recognizing actin (1:1000). FEBS-Letters mint wasf1-gnb1 2 6 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F3A The interaction is regulated by the presence of GTPγS. F3:Rat brain lysates (2 mg) (A)were preincubated with 100 μM GDP or 100 μM GTPγS and 25 mM MgCl2 at 24 °C for 30 min prior to immunoprecipitation. Membrane transfers were first blotted with Giα3 antisera (1:5000) and then stripped and sequentially reprobed with antibodies or sera recognizing Gβ (1:5000). FEBS-Letters mint wasf1-gnb1-1 1 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F3B F3: COS-7 cells lysates (B) were preincubated with 100 μM GDP or 100 μM GTPγS and 25 mM MgCl2 at 24 °C for 30 min prior to immunoprecipitation. Membrane transfers were first blotted with Giα3 antisera (1:5000) and then stripped and sequentially reprobed with antibodies or sera recognizing Gβ (1:5000). FEBS-Letters mint wasf1-gnai3-1 1 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q5BJU7:p.Gln517Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A Tue Jul 12 10:23:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:03:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln517ala' Tue Jun 30 16:40:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln517Ala' range MINT-1795858: original sequence: 517-517 : KGIQLRKVEEQREQEAKHER flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell Stoichiometry: 1.0 physical association physical association F3B The interaction is regulated by the presence of GTPγS. The specificity of the interaction between WAVE1 and Giα3 was further addressed by cotransfection of COS7 cells with FLAG-tagged WAVE1 and Giα3. Transfected Giα3 and WAVE1 also coimmunoprecipitated and this was disrupted by addition of GTPγS (see Fig. 3B). FEBS-Letters mint wasf1-actb-1 1 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5BJU7:p.Gln517Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A Tue Jul 12 10:22:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:03:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln517ala' Tue Jun 30 19:44:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln517Ala' range MINT-1795873: original sequence: 517-517 : KGIQLRKVEEQREQEAKHER Stoichiometry: 1.0 7 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F3B The interaction is regulated by the presence of GTPγS and occurs only if also Gialpha3 is overexpressed. COS-7 cells lysates (B) were preincubated with 100 μM GDP or 100 μM GTPγS and 25 mM MgCl2 at 24 °C for 30 min prior to immunoprecipitation. Membrane transfers were first blotted with Giα3 antisera (1:5000) and then stripped and sequentially reprobed with antibodies or sera recognizing actin (1:1000), p34 Arc (1:1000). FEBS-Letters mint wasf1-arpc2 1 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q5BJU7:p.Gln517Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A Tue Jul 12 10:57:14 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:52:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln517ala' Tue Jun 30 18:00:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln517Ala' range MINT-1795906: original sequence: 517-517 : KGIQLRKVEEQREQEAKHER flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 3 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 physical association physical association F3B The interaction is regulated by the presence of GTPγS and occurs only if also Gialpha3 is overexpressed. COS-7 cells lysates (B) were preincubated with 100 μM GDP or 100 μM GTPγS and 25 mM MgCl2 at 24 °C for 30 min prior to immunoprecipitation. Membrane transfers were first blotted with Giα3 antisera (1:5000) and then stripped and sequentially reprobed with antibodies or sera recognizing p34 Arc (1:1000). FEBS-Letters mint