MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. EMBO J. (0261-4189) 2006 imex curation Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. Only protein-protein interactions in vitro In vitro cosedimentation cosedimentation nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. EMBO J. (0261-4189) 2006 imex curation Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. EMBO J. (0261-4189) 2006 imex curation Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. EMBO J. (0261-4189) 2006 imex curation Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. Adaptor protein controlled oligomerization activates the AAA+ protein ClpC. EMBO J. (0261-4189) 2006 imex curation Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kirstein J., Schlothauer T., Dougan DA., Lilie H., Tischendorf G., Mogk A., Bukau B., Turgay K. EMBO J. (0261-4189) 2006 Only protein-protein interactions imex curation comk_bacsu Competence transcription factor comK Competence protein K BSU10420 protein protein bacsu Bacillus subtilis MSQKTDAPLESYEVNGATIAVLPEEIDGKICSKIIEKDCVFYVNMKPLQIVDRSCRFFGSSYAGRKAGTYEVTKISHKPPIMVDPSNQIFLFPTLSSTRPQCGWISHVHVKEFKATEFDDTEVTFSNGKTMELPISYNSFENQVYRTAWLRTKFQDRIDHRVPKRQEFMLYPKEERTKMIYDFILRELGERY mint Intermediate regulatory gene required for the expression of the late competence genes comC, comE, comG and the bdbDC operon. Receives signals from srfA, and possibly other regulatory COM genes, and transduces these signals to the late COM genes E028FC1F57D5736D meca1_bacsu Adapter protein MecA 1 mecA BSU11520 protein protein bacsu Bacillus subtilis MEIERINEHTVKFYMSYGDIEDRGFDREEIWYNRERSEELFWEVMDEVHEEEEFAVEGPLWIQVQALDKGLEIIVTKAQLSKDGQKLELPIPEDKKQEPASEDLDALLDDFQKEEQAVNQEEKEQKLQFVLRFGDFEDVISLSKLNVNGSKTTLYSFENRYYLYVDFCNMTDEEVENQLSILLEYATESSISIHRLEEYGKLIISEHALETIKKHFAS F6E377D8EA241B7D F6E377D8EA241B7D clpp_bacsu ATP-dependent Clp protease proteolytic subunit clpP BSU34540 yvdN Endopeptidase Clp Caseinolytic protease Stress protein G7 protein protein bacsu Bacillus subtilis MNLIPTVIEQTNRGERAYDIYSRLLKDRIIMLGSAIDDNVANSIVSQLLFLAAEDPEKEISLYINSPGGSITAGMAIYDTMQFIKPKVSTICIGMAASMGAFLLAAGEKGKRYALPNSEVMIHQPLGGAQGQATEIEIAAKRILLLRDKLNKVLAERTGQPLEVIERDTDRDNFKSAEEALEYGLIDKILTHTEDKK 5D4D4BD548DC45A0 mint Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). ClpXP is involved in the complete degradation of the Site-2 clipped anti-sigma-W factor rsiW. This results in the release of sigW and the transcription activation of the genes under the control of the sigma-W factor 5D4D4BD548DC45A0 clpc_bacsu Negative regulator of genetic competence ClpC/MecB clpC mecB BSU00860 protein protein bacsu Bacillus subtilis MMFGRFTERAQKVLALAQEEALRLGHNNIGTEHILLGLVREGEGIAAKALQALGLGSEKIQKEVESLIGRGQEMSQTIHYTPRAKKVIELSMDEARKLGHSYVGTEHILLGLIREGEGVAARVLNNLGVSLNKARQQVLQLLGSNETGSSAAGTNSNANTPTLDSLARDLTAIAKEDSLDPVIGRSKEIQRVIEVLSRRTKNNPVLIGEPGVGKTAIAEGLAQQIINNEVPEILRDKRVMTLDMGTVVAGTKYRGEFEDRLKKVMDEIRQAGNIILFIDELHTLIGAGGAEGAIDASNILKPSLARGELQCIGATTLDEYRKYIEKDAALERRFQPIQVDQPSVDESIQILQGLRDRYEAHHRVSITDDAIEAAVKLSDRYISDRFLPDKAIDLIDEAGSKVRLRSFTTPPNLKELEQKLDEVRKEKDAAVQSQEFEKAASLRDTEQRLREQVEDTKKSWKEKQGQENSEVTVDDIAMVVSSWTGVPVSKIAQTETDKLLNMENILHSRVIGQDEAVVAVAKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESIFGDEESMIRIDMSEYMEKHSTSRLVGSPPGYVGYDEGGQLTEKVRRKPYSVVLLDEIEKAHPDVFNILLQVLEDGRLTDSKGRTVDFRNTILIMTSNVGASELKRNKYVGFNVQDETQNHKDMKDKVMGELKRAFRPEFINRIDEIIVFHSLEKKHLTEIVSLMSDQLTKRLKEQDLSIELTDAAKAKVAEEGVDLEYGARPLRRAIQKHVEDRLSEELLRGNIHKGQHIVLDVEDGEFVVKTTAKTN 9592231886C2E870 mint Competence gene repressor; required for cell growth at high temperature. Negative regulator of comK expression. May interact with mecA to negatively regulate comK 9592231886C2E870 clpc-meca 3 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 direct interaction direct interaction F1B ClpC and mecA form a heterodimeric complex mint clpc-comk 3 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified mbp tagged mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 association association F4A complex conteinin clpC, mecA and ComK-MBP mint clpc-clpp-2 3 8 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 association association F4B complex consisting of clpC, mecA and ClpP mint clpc-meca-1 1 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 direct interaction direct interaction F2A ClpC and mecA form a heterodimeric complex mint clpc-meca-2 4 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 direct interaction direct interaction SF1A ClpC and mecA form a heterodimeric complex mint clpc-meca-3 2 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 colocalization colocalization SF1B ClpC and mecA form a heterodimeric complex mint meca-clpc 5 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Stoichiometry: 1.0 direct interaction direct interaction F5 ClpC and mecA form a heterodimeric complex mint clpc-comk-1 5 6 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 9 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 7 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 physical association physical association mint