IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A proteomic analysis of 14-3-3 binding proteins from developing barley grains. A proteomic analysis of 14-3-3 binding proteins from developing barley grains. Proteomics (1615-9853) 2006 imex curation Alexander RD., Morris PC. Corresponding author contacted regarding incorrect GI identifiers. Revisit when true GI numbers supplied. Extracts were obtained from developing barley grains 14 to 21 days post anthesis and isolated starch granules. Table 1 Originally 52 protein GI accession numbers were provided in Table 1. These were extracted from the text and mapped to Uniprot accession numbers using a perl script. Three GI numbers were incorrect, identifiers ACC synthase GI:112713 identifies a protein from winter squash not barley as stated in Table 1. This is the case for GI:339055 Photosystem 1 reaction centre subunit psaK identifies a human protein and alpha amylase inhibitor GI:578524 which identifies a protein from Tetrahymena capricornis, Chlorophyll ab binding protein GI:3153150 which identifies the protein in Acetabularia mediterranea. One protein were not represented in Uniprot GI:23476275 Soluble starch synthase II. 13 proteins were identified as homologues in rice, arabidopsis and spinach. After correspondence with the authors 1 more protein was added, but four proteins identities remained unresolved. p.c.morris@hw.ed.ac 100..100 100..110 2006-05-19: Only interaction involving solely Proteins were exported. Only protein-protein interactions 1 in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom partial id prot seq partial identification of protein sequence partial id prot seq Alexander RD., Morris PC. Corresponding author contacted regarding incorrect GI identifiers. Revisit when true GI numbers supplied. Extracts were obtained from developing barley grains 14 to 21 days post anthesis and isolated starch granules. Extracts were obtained from developing barley grains 14 to 21 days post anthesis and isolated starch granules. Table 1 Originally 52 protein GI accession numbers were provided in Table 1. These were extracted from the text and mapped to Uniprot accession numbers using a perl script. Three GI numbers were incorrect, identifiers ACC synthase GI:112713 identifies a protein from winter squash not barley as stated in Table 1. This is the case for GI:339055 Photosystem 1 reaction centre subunit psaK identifies a human protein and alpha amylase inhibitor GI:578524 which identifies a protein from Tetrahymena capricornis, Chlorophyll ab binding protein GI:3153150 which identifies the protein in Acetabularia mediterranea. One protein were not represented in Uniprot GI:23476275 Soluble starch synthase II. 13 proteins were identified as homologues in rice, arabidopsis and spinach. After correspondence with the authors 1 more protein was added, but four proteins identities remained unresolved. p.c.morris@hw.ed.ac Accepted 2006-APR-25 by JYOTI. Proteomics (1615-9853) 2006 100..100 100..110 2006-05-19: Only interaction involving solely Proteins were exported. Only protein-protein interactions 1 imex curation adh2_horvu Alcohol dehydrogenase 2 ADH2 protein protein horvu Hordeum vulgare (Barley) Barley MATAGKVIKCKAAVAWEAGKPLSMEEVEDAPPQAMEVRDKILYTALCHTDVYFWEANGQTPVFPRILGHEAGGIVESVGEGVTELVPGDHVLPVFTGECKECAHCMSEESNLCDLLRINVDRGVMIDDGQSRFTIDGKPIFHFLGTSTFSEYTVIHVGCVAKIDPEAPLDKVCLLSCGISTGLGATLNVTKPKKGMTVAIFGLGAVGLAAMEGARMSGASRIIGVDLNPAKHEQAKKFGCTDFVNPKDHTKPVQEVIVEMTDGGVNRAVECTGNADAMISAFECVHDGWGVAHKEAVFKTHPMNFLNERTLRGTFFGNYKPRTGLPGVVDMYMRKELELDKFITHSLPFSQINTAFDLMLRGEGLRCVIRSEE 97EA42A3EA3937E9 pdi_horvu Protein disulfide-isomerase PDI Endosperm protein E-1 protein protein horvu Hordeum vulgare (Barley) Barley MAISKVWISLLLALAVVLSAPAARAEEAAAAEEAAAPEAVLTLHADNFDDAIGQHPFILVEFYAPWCGHCKSLAPEYEKAAQLLSKHDPAIVLAKVDANDEKNKPLAGKYEVQGFPTLKIFRNGGKSIQEYKGPREAEGIVEYLKKQVGPASKEIKAPEDATYLEDGKIHIVGVFTEFSGPEFTNFLEVAEKLRSYYDFGHTVHANHLPRGDAAVERPVVRLFKPFDELVVDSKDFDVSALEKFIDASSTPKVVIFDKNPDNHPYLLKFFQSNAPKAMLFLNFSTGPFESFKSAYYGAVEEFSGKDVKFLIGDIESSQGAFQYFGLKVDQAPLILIQDGDSKKFLKEHVEAGQIVAWLKDYFDGKLTPFRKSEPIPEANNEPVKVVVADNVHDVVFKSGKNVLIEFYAPWCGHCKKLAPILDEAAATLQSEEDVVIAKMDATENDVPGEFDVQGYPTLYFVTPSGKKVSYEGGRTADEIVDYIRKNKETAGQAAAATEKAAEPAATEPLKDEL 03BBAE2EE164F972 q8l4x2_horvu RGH2b RGH2a protein protein horvu Hordeum vulgare (Barley) Barley MYGVWFASGRNMEMMEAPVSASQGAMRSLPRKLESLLSGPNQGLRPEEKKKLRFLQADLQGLIDSYLFEPSEVESPASTASFWMKDVRDLSYDIDDFIYELGHVAVSGARIGAAQIQKVPGVKISRFPDKLKRRQWISDEISGFRTRVKEAIQRHKTYLGGCRWRPSSSSSQHSAPSPCGRAAIRLVGMDSPVEQLCGWLANDGQPEHKVASIVGTSGVGKTTLARQVYHKLGGQFECRAFVQASPKQDMKMLLTSILSQVRRHHLPDSFDVHKLLFEIEAHLQDKTYIIVIDNLQASSTWDIINHALPKENCCSRILTTTEVDAIAQTCCADTSKYIFKKESLSEDESRELFLGTVFGHEAGCPQVLKEVSNEIIKRSGGLPLAIIILASLLVGQPASSIEHWNHIKNSLSSDSSTNTSLEGIKQVLNLGYEYLPHYLKACMLYLCMYEEDCIIWKDDLVKQWIAEGFIHAMDGNGGKEVARSYFDELVNRGMIQPMDINCNDEILSFSVHTMVLHFIRYKSVEENFSIALDHSQTTIRLADKVRRLALHFGNVEHATPPMPASMRLSQVRSLAFSGLLKCMPSFVEFRFIQVLILKLWADPDNRSDNFIGSDDLSGNITEPDDLSDNLTEHDDLSLNLSEISELFRLRYFHLDACHMSVELPTQMKRLKDLVACEIDANVTAVPSDIVDLPGLFFLSIPSEARLPTGIGRMTSLCTLGIIDLSNNSTENIMCLGELTNLQDLRLTCSTLQADNLEKNLECLGLIIRKLSNLKCVTLVPVVSSHVKTQDDASSSRMTISWDGFTTVSLSPALLQRLELSRRCFILSSLPEWTKDLTNLGILKMAVSKVSSEDVVILKGLPSLTALSLFVWTAPTRRIIFDNEGFLVLKYFKFVCVAPCLSFLDGAMPKVHNLKLGFNANRMEQYSLVAAGFEHLIGLKKISIKIGGAGADECDRRSAQLVLTDAISKHPNTSIISVQWVNSKFCGEQDVNAGESSSYQQDTGNLLFPSHLTLCPFNSVCC 3AB181D086516D27 fdh_horvu Formate dehydrogenase, mitochondrial NAD-dependent formate dehydrogenase protein protein horvu Hordeum vulgare (Barley) Barley MAAMWRAAARQLVDRAVGSRAAHTSAGSKKIVGVFYQAGEYADKNPNFVGCVEGALGIRDWLESKGHHYIVTDDKEGFNSELEKHIEDMHVLITTPFHPAYVTAEKIKKAKTPELLLTAGIGSDHIDLPAAAAAGLTVARVTGSNTVSVAEDELMRILILLRNFLPGYQQVVKGEWNVAGIAHRAYDLEGKTVGTVGAGRYGRLLLQRLKPFNCNLLYHDRLQINPELEKEIGAKFEEDLDAMLPKCDVVVINTPLTEKTRGMFNKEKIAKMKKGVIIVNNARGAIMDTQAVADACSSGHIAGYGGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQLRYAAGVKDMLDRYFKGEEFPVENYIVKEGELASQYK E28C1FE24E9225C7 ino1_horvu Inositol-3-phosphate synthase Myo-inositol 1-phosphate synthase protein protein horvu Hordeum vulgare (Barley) Barley MFIESFRVESPKVRYGAGEIESEYRYDTTELVHESHDGASKWVVRPKSVNYHFKTNTTVPKLGVMLVGWGGNNGSTLMAGVIANREGISWATKDKVQQANYFGSLTQASTIRVGSYNGEEIYAPFKSLLPMVNPDDLVFGGWDISSMNLADAMTRAKVLDIDLQKQLRPYMESMCPLPGIYDPDFIAANQGSRANNVIKGTKKEQMEQVIKDIREFKEKNKVDKVVVLWTANTERYSNVSVGLNDTTENLLASVDKNEAEISPSTLYAIACVMEGVPFINGSPQNTFVPGLIDLAIKNDCLIGGDDFKSGQTKMKSVLVDFLVGAGIKPTSIVSYNHLGNNDGMNLSAPQTFRSKEISKSNVVDDMVSSNAILYEPGEHPDHVVVIKYVPYVGDSKRAMDEYTSEIFMGGKSTIVLHNTCEDSLLRAPIILDLVLLAELSTRIQLKAEGEDKLHSFHPVATILSYLTKAPLVPPGTPVVNALAKQRAMLENIMRACVGLAPENNMILEYK EA63138121692724 tpis_horvu Triosephosphate isomerase, cytosolic protein protein horvu Hordeum vulgare (Barley) Barley MGRKFFVGGNWKCNGTVEQVEAIVQTLNAGQIVSPDVVEVVVSPPYVFLPIVKAKLRPEIQVAAQNCWVKKGGAFTGEVSAEMLANLGVPWVILGHSERRSLLGESSEFVGEKVAYALAQGLKVIACVGETLEQREAGSTMEVVAEQTKAIAGKIKDWSNGVVAYEPVWAIGTGKVATPAQAQEVHANLRDWLKTNVSPEVAESTRIIYGGSVTGASCKELAAQADVDGFLVGGASLKPEFIDIINAAAVKSA 023B216A6577EA5F o81135_horvu LTP-ne protein protein horvu Hordeum vulgare (Barley) Barley MAMRKTKELLLVAMMLALVVAARAAPCEVGQLTVCMPAITTGAKPSGACCANLGAQQGCFCQYAKDPALGRYITSPHARDTLLSCGLAVPRC B6A3F562FF6EC2D9 q42847_horvv Lipoxygenase LoxC protein protein horvd Hordeum vulgare var. distichum (Two-rowed barley) MLGVGGIVSDLTGGIRGAHLKGSVVLMRKNALDFNDFGAHVMDGVTELLGRGVTCQLISSTNVDHNNGGRGKVGAEANLEQWLLPTNLPFITTGENKFAVTFDWSVDKLGVPGAIIVKNNHASEFFLKTITLDNVPGRGTIVFVANSWVYPQAKYRYNRVFFANDTYLPHQMPAALKPYRDDELRNLRGDDQQGPYLDHDRVYRYDVYNDLGDSRDVLGGSKDLPYPRRCRTGRKPSDSKPDHESRLLLLVQNVYVLRDELFGHLKQSDLLGYTLKGWLDGIILAIRTYVDLSPGEFDSFADILKLYEGGIKLPNIPALEEVRKRFPLQLVKDLIPKGGDFLLKLPKPEIIKVDQKAWMTDEEFAREMLAGVNPMMIKRLTEFPPKSTLDPSKYGDHTSTMTEEHVAKSLEGLTVQQALAGNRLYIVDQHDNLMPFLIDINNLDASFVYATRTLLFLRGDGTLAPVAIELSSPLIQGELTTAKSAVYTPQHAGVEGWIWQLAKAYASVNDYGWHQLISHWLNTHAVMEPFVIATNRQLSVTHPVYKLLHPHYRDTMNINARARGLLINAGGVIEMTVFPHKHAMPMSSMVYKHWNFTEQALPADLIKRGMAVEDASSPHKVRLLIKDYPYATDGLAVWDAIEQWVSDYLTIYYPNDGVLQGDVELQAWWKEVREVGHGDLKDAAWWPKMQTVAELIKACATIIWTGSALHAAVNFGQYPYSGYHPNKPSASRRPMPVQGSEEYAELERDPEKAFIRTITSQFHALVGISLMEILSKHSSDEVYLGQHDTPAWTSDAKALEAFKRFGAKLEGIEKQVVAMNSDPQLKNRTGPAKFPYMLLYPNTSDHTGQAEGLTARGIPNSISI A15DA282FB7F9973 amy6_horvu Alpha-amylase type B isozyme AMY1.6 1,4-alpha-D-glucan glucanohydrolase Clones GRAMY56 and 963 protein protein horvu Hordeum vulgare (Barley) Barley MANKHMSLSLFIVLLGLSCSLASGQVLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGVTHVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKAVKAIADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYPDGTGNRPTRTRADFGAAPDIDHLNPRVQKELVEWLNWLRTDDGFDGWRFDFAKGYSADVAKIYVDRSEPSFAVAEIWTSLAYGGDGKPNLNQDPHRQELVNWVNKVGGSGPATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPGNPCIFYDHFFDWGLKEEIDRLVSIRTRQGIHSESKLQIMEADADLYLAEIEGKVIVKLGPRYDVGHLIPEGFKVVAHGNDYAVWEKV 1C924CA6319D5262 g3pc2_horvu Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic GAPC protein protein horvu Hordeum vulgare (Barley) Barley VNDPFITTDYMTYMFKYDTVHGQWKHHEVKVKDSKTLLFGEKEVAVFGCRNPEEIPWAAAGAEYVVESTGVFTDKDKAAAHIKGGAKKVIISAPSKDAPMFVCGVNEKEYKSDIDIVSNASCTTNCPAPLAKVINDRFGIVEGLMTTVHAMTATQKTVDGPSSKDWRGGRAASFNIIPSSTGAAKAVGKVLPELNGKLTGMAFRVPTVDVSVVDLTVRLAKPATYEQIKAAIKEESEGNLKGILGYVDEDLVSTDFQGDSRSSIFDAKAGIALNDNFVKLVSWYDNEWGYSTRVVDLIRHMHSTK 4F82FA362F222AC5 q42852_horvu Peroxidase protein protein horvu Hordeum vulgare (Barley) Barley SSNVQSLLALLGRLGLDATDLVTISGGHTIGLAHCSSFEDRLFPRPDPTISPTFLSRLKRTCPAKGTDRRTVLDVRTPNVFDNKYYIDLVNREGLFVSDQDLFTNAITRPIVERFAQSQQDFFEQFGVSIGKMGQMRVRTSDQGEVRRNCSVRNPGPGADALQLPSLVQTIVDEAAGSIG B3F60662702AC8F6 o65817_horvu Chlorophyll a-b binding protein, chloroplastic CP29 protein protein horvu Hordeum vulgare (Barley) Barley LYPGGSYFDPLGLAADPEKKETLQLAEIKHARLAMVAFLGFAVQAAATGKGPLNNWATHLSDPLHTTIFDTFGSS 5442D30B9E61057D q9ztb7_horvu 1,4-alpha-glucan branching enzyme sbeIIa protein protein horvu Hordeum vulgare (Barley) Barley MAEVNMTGGAAEKLESSEPTQGIAETITDGVTKGVKELVVGEKPQVVPKPGDGQKIYEIDPTLKDFRSHLDYRYSEYKRIRAAIDQHEGGLEVFSRGYEKLGFTRSAKGITYREWAPGAHSAALVGDFNNWNPNADTMTRDDYGVWEIFLPNNADGSPAIPHGSRVKIRMDTPSGVKDSISAWIKFSVQAPGEIPFNGIYYDPPEEEKYVFQHPQPKRPESLRIYESHIGMSSPEPKINSYANFRDEVLPRIKRLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPEDLKSLIDRAHELGLLVLMDIVHSHSSNNTLDGLNGFDGTDTHYFHGGPRGHHWMWDSRLFNYGSWEVLRFLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQMTFTGNYGEYFGFATDVDAVVYLMLVNDLIHGLYPDAVSIGEDVSGMPTFCIPVPDGGVGFDYRLHMAVADKWIELLKQSDESWKMGDIVHTLTNRRWLEKCVTYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPSTPRIDRGIALHKMIRLVTMGLGGEGYLNFMGNEFGHPEWIDFPRGPQTLPTGKVLPGNNNSYDKCRRRFDLGDADFLRYRGMQEFDQAMQHLEEKYGFMTSEHQYVSRKHEEDKVIIFERGDLVFVFNFHWSNSKKDYRVGCSKPGKYKVALDSDDALFGGFSRLDHDVDYFTTEHPHDNRPRSFSVYTPSRTAVVYALTE DE4F0EEDB0ACAF93 q9m5a3_horvu Starch synthase, chloroplastic/amyloplastic SSI protein protein horvu Hordeum vulgare (Barley) Barley MAATGVGAGCLAPSVRLRADPAARATACVVRARLRRVARGRYVAELSREGPAARPAQQLAPPVVPGFLAPPPPAPAQSPAPTQPPLPDAGVGELAPDLLLEGIAEDSIDTIVVAASEQDSEIMDANDQPLAKVTRSIVFVTGEAAPYAKSGGLGDVCGSLPIALAARGHRVMVVMPRYLNGTSDKNYAKALYTGKHIKIPCFGGSHEVTFFHEYRDNVDWVFVDHPSYHRPGSLYGDNFGAFGDNQFRYTLLCYAACEAPLILELGGYIYGQSCMFVVNDWHASLVPVLLAAKYRPYGVYRDSRSTLVIHNLAHQGVEPASTYPDLGLPPEWYGALEWVFPEWARRHALDKGEAVNFLKGAVVTADRIVTVSQGYSWEVTTAEGGQGLNELLSSRKSVLNGIVNGIDINDWNPTTDKCLPHHYSVDDLSGKAKCKAELQRELGLPVREDVPLIGFIGRLDYQKGIDLIKMAIPDLMREDVQFVMLGSGDPVFEGWMRSTESSYKDKFRGWVGFSVPVSHRITAGCDILLMPSRFEPCGLNQLYAMQYGTVPVVHGTGGLRDTVETFNPFGAKGEEGTGWAFSPLTVEKMLWALRTAISTFREHKPSWEGLMKRGMTKDHTWDHAAEQYEQIFEWAFVDQPYVM C3842A78AF5220EB q9fuk7_horvu Beta-amylase protein protein horvu Hordeum vulgare (Barley) Barley VNVKGNYVQVYVMLPLDAVSVNNRFEKGDELRAQLRKLVEAGVDGVMVDVWWGLVEGKGPKAYDWSAYKQLFELVQKAGLKLQAIMSFHQCGGNVGDAVNIPIPQWVRDVGTRDPDIFYTDGHGTRNIEYLTLGVDNQPLFHGRSAVQMYADYMTSFRENMKDFLDAGVIVDIEVGLGPAGEMRYPSYPQSHGWSFPGIGEFICYDKYLQADFKAAAAAVGHPEWEFPNDVGQYNDTPERTQFFRDNGTYLSEKGRFFLAWYSNNLIKHGDRILDEANKVFLGYKVQLAIKISGIHWWYKVPSHAAELTAGYYNLHDRDGYRTIARMLKRHRASINFTCAEMRDLEQSSQAMSAPEELVQQVLSAGWREGLNVACENALPRYDPTAYNTILRNARPHGINQSGPPEHKLFGFTYLRLSNQLVEGQNYVNFKTFVDRMHANLPRDPYVDPMAPLPRSGPEISIEMILQAAQPKLQPFPFQEHTDLPVGPTGGMGGQAEGPTCGMGGQVKGPTGGMGGQAEDPTSGIGGELPATM 793031ED53FFACB5 q84ng8_horvu protein protein horvu Hordeum vulgare (Barley) Barley METRPWLLRLIAILTTTTALLLHPSTSSSVSTAHDLPALLSFKSLITKDPLGALSSWTTNGSTHGFCSWTGVECSSAHPGHVKALRLQGLGLSGTISPFLGNLSRLRALDLSGNKLQGQIPSSIGNCFALRTLNLSVNSLSGAIPPAMGNLSKLLVLSVSKNDISGTIPTSFAGLATVAVFSVARNHVHGQVPPWLGNLTALEDLNMADNIMSGHVPPALSKLINLRSLTVAINNLQGLIPPVLFNMSSLECLNFGSNQLSGSLPQDIGSMLPNLKKFSVFYNRFEGQIPASLSNISSLEHLSLHGNRFRGRIPSNIGQSGRLTVFEVGNNELQATESRDWDFLTSLANCSSLLLVNLQLNNLSGILPNSIGNLSQKLEGLRVGGNQIAGLIPTGIGRYLKLAILEFADNRFTGTIPSDIGKLSNLKELSLFQNRYYGEIPSSIGNLSQLNLLALSTNNLEGSIPATFGNLTELISLDLASNLLSGKIPEEVMRISSLALFLNLSNNLLDGPISPHIGQLANLAIIDFSSNKLSGPIPNALGSCIALQFLHLQGNLLQGQIPKELMALRGLEELDLSNNNLSGPVPEFLESFQLLKNLNLSFNHLSGPVPDKGIFSNASVISLTSNGMLCGGPVFFHFPTCPYPSPDKLASHKLLQILVFTAVGAFILLGVCIAARCYVNKSRGDAHQDQENIPEMFQRISYTELHSATDSFSEENLVGRGSFGSVYKGTSGSGANLITAAVKVLDVQRQGATRSFISECNALKMIRHRKLVKVITVCDSLDHSGNQFKALVLEFIPNGSLDKWLHPSTEDEFGTPNLMQRLNIALDVAEALEYLHDHIDPPIVHCDVKPSNILLDDDMVAHLGDFGLAKIIRAEKSKQSLADQSCSVGIKGTIGYVAPEYGTGTEISVEGDVYSYGVLLLEMLTGRRPTDPFFSDTTNLPKYVEMACPGNLLETMDVNIRCNQEPQAVLELFAAPVSRLGLACCRGSARQRIKMGDVVKELGAIKQIIMASQNYASWSTKLY 09EB62D3C41141C6 sus2_horvu Sucrose synthase 2 SS2 Sucrose-UDP glucosyltransferase 2 protein protein horvu Hordeum vulgare (Barley) Barley MGETAGERALSRVHSVRERIGHSLSAHTNELVAVFSRLVNQGKGMLQPHQITAEYNAAIPEAEREKLKNTPFEDLLRGAQEAIVIPPWVALAIRPRPGVWEYVRVNVSELGVEELSVLRYLQFKEQLANGSTDNNFVLELDFGPFNASFPRPSLSKSIGNGVQFLNRHLSSKLFHDKESMYPLLNFLRAHNYKGMTMMLNDRIRSLGTLQGALRKAETHLSGLPADTPYTEFHHRFQELGLEKGWGDCAQRASETIHLLLDLLEAPDPSSLEKFLGTIPMVLNVVILSPHGYFAQANVLGYPDTGGQVVYILDQVRAMENEMLLRIKQQGLDITPKILIVTRMLPDAHGTTCGQRLEKVLGTEHTHILRVPFKTEDGIVRKWISRFEVWPYLEAYTDDVAHEIAGELQANPDLIIGNYSDGNLVACLLAHKLGVTHCTIAHALEKTKYPNSDLYWKKFEDHYHFSCQFTADLIAMNHADFIITSTFQEIAGNKDTVGQYESHMAFTMPGLYRVVHGIDVFDPKFNIVSPGADMSIYFPYTEQQKRLTSLHTEIEELLFSDVENAEHKFVLKDKKKPIIFSMARLDRVKNMTGLVEMYGRNPRLQELVNLVVVCGDHGKVSKDKEEQVEFKKMFDLIEKYNLSGHIRWISAQMNRVRNGELYRYICDMKGAFVQPAFYEAFGLTVIEAMTCGLPTFATAYGGPAEIIVNGVSGYHIDPYQNDKASALLVGFFGKCQEDPSHWNKISQGGLQRIEEKYTWKLYSERLMTLSGVYGFWKYVSNLDRRETRRYLEMLYALKYRKMAATVPLAVEGETSGE CB90A9C42AAB1080 q84kc6_horvu rga S-9201 protein protein horvu Hordeum vulgare (Barley) Barley MAHVAGLLASAVVSAVGNKLGSAIGDEVTMLWSFKDDLKDMKDTLESMEAVLKDAERRSVKEELVRLWLNRLKHAAYDISYMLDEFQANSEPASRKMIGKLDCFAIAPKITLAYKMKKMRGQLRKIKEDHESFKFTHANSSLINVHQLPDPRETSSNVVESLIIGREKDRMNVLSLLSTSNNIKEDFTVLPICGLGGIGKTTLAQLVFNDAQFNDYHRVWVYVSQVFDLNKIGNSIISQVSGKGSEHSHTLQHISKQLKDLLQDKKTLIVLDDLWETGYFQLDQLKLMLNVSTKMKVLVTTRSIDIARKMGNVGVEPYMLDPLDNDMCWRIIKQSSRFQSRPDKEQLEPNGQKIARKCGGLPLAAQALGFLLSGMDLSEWEAICISDIWDEPFSDSTVLPSLKLSYNTLTPYMRLCFAYCGIFPKGHNISKDYLIHQWIALGFIEPSNKFSAIQLGGKYVRQFLGMSFLHHSKLPETFGNAMFTMHDLVHDLARSVITEELVVFDAEIVSDNRIKEYCIYASLTNCNISDHNKVRKMTTIFPPKLRVMHFSDCKLHGSAFSFQKCLRVLDLSGCSIKDFASALGQLKQLEVLIAQKLQDRQFPESITRLSKLHYLNLSGSRGISEIPSSVGKLVSLVHLDLSYCTNVKVIPKALGILRNLQTLDLSWCEKLESLPESLGSVQNLQRLNLSNCFELEALPESLGSLKDVQTLDLSSCYKLESLPESLGSLKNVQTLDLSRCYKLVSLPKNLGRLKNLRTIDLSGCKKLETFPESFGSLENLQILNLSNCFELESLPESFGSLKNLQTLNLVECKKLESLPESLGGLKNLQTLDFSVCHKLESVPESLGGLNNLQTLKLSVCDNLVSLLKSLGSLKNLQTLDLSGCKKLESLPESLGSLENLQILNLSNCFKLESLPESLGRLKNLQTLNISWCTELVFLPKNLGNLKNLPRLDLSGCMKLESLPDSLGSLENLETLNLSKCFKLESLPESLGGLQNLQTLDLLVCHKLESLPESLGGLKNLQTLQLSFCHKLESLPESLGGLKNLQTLTLSVCDKLESLPESLGSLKNLHTLKLQVCYKLKSLPESLGSIKNLHTLNLSVCHNLESIPESVGSLENLQILNLSNCFKLESIPKSLGSLKNLQTLILSWCTRLVSLPKNLGNLKNLQTLDLSGCKKLESLPDSLGSLENLQTLNLSNCFKLESLPEILGSLKKLQTLNLFRCGKLESLPESLGSLKHLQTLVLIDCPKLEYLPKSLENLSGNRF B833D10CBFAF76B6 spz4_horvu Serpin-Z4 PAZ1 HorvuZ4 BSZ4 Protein Z4 Major endosperm albumin protein protein horvu Hordeum vulgare (Barley) Barley MATTLATDVRLSIAHQTRFALRLRSAISSNPERAAGNVAFSPLSLHVALSLITAGAAATRDQLVAILGDGGAGDAKELNALAEQVVQFVLANESSTGGPRIAFANGIFVDASLSLKPSFEELAVCQYKAKTQSVDFQHKTLEAVGQVNSWVEQVTTGLIKQILPPGSVDNTTKLILGNALYFKGAWDQKFDESNTKCDSFHLLDGSSIQTQFMSSTKKQYISSSDNLKVLKLPYAKGHDKRQFSMYILLPGAQDGLWSLAKRLSTEPEFIENHIPKQTVEVGRFQLPKFKISYQFEASSLLRALGLQLPFSEEADLSEMVDSSQGLEISHVFHKSFVEVNEEGTEAGAATVAMGVAMSMPLKVDLVDFVANHPFLFLIREDIAGVVVFVGHVTNPLISA 023B8E52475ABA56 q9lkw4_horvu UDP-glucose-fructose-phosphate glucosyltransferase protein protein horvu Hordeum vulgare (Barley) Barley LEEELSLDASEIVIASTRQEIEEQWNLYDGFEVILARKLRARVKRGANCYGRYMPRMVIIPPGVEFGHIVHDFDIDGEEENHGPASEDPPIWSQIMRFFTNPRKPMILAVARPYPEKNITTLVKAFGECRPLRELANLTLIMGNREAISKMHNTSASVLTSVLTLIDEYDLYGQVAYPKHHKHSEVPDIYRLATRTKGAFVNVAYFEQFGVTLIEAAMNGLPVIATKNGAPVEIHQVLNNGLLVDPHDQNAIADALYKLLSEKQLWSRCRENGLKNIHQFSWPEHCKNHLSRILTLGMRSPAVGSKEERSKAPISGRKHIIVISVDSVNKEDLVRIIRNAIEAAHTENTPALTGFVLSTSLTISEICSLLVSVGMHPAGFDAFICNSGSSIYYPSYSGNTPSNSKVTHVIDRNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRIERQMIFEDSEHSSTYCLAFKVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVTPIHASRSQAIRYLFVRWGIELPNIVVIVGESGDSDYEELLGGLHRTIILKGDFNIAANRIHTVRRYPLQDVVALDSSNIIEVQGCTTEDIKSALRHIGVPTQ 5DCA523130B47170 1433a_horvu 14-3-3-like protein A protein protein horvu Hordeum vulgare (Barley) Barley MSTAEATREENVYMAKLAEQAERYEEMVEFMEKVAKTADVGELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEAYVASIKEYRTRIETELSKICDGILKLLDSHLVPSATAAESKVFYLKMKGDYHRYLAEFKAGAERKEAAENTLVAYKSAQDIALADLPTTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDSLGEESYKDSTLIMQLLRDNLTLWTSDNAEEGGDEIKEAASKPEGEGHS DAE722EA5D416DAA q9lly2_horvu AGL97 protein protein horvu Hordeum vulgare (Barley) Barley MATRSLLLLCLCLCLFAPRLCSSKEEGPLAAGGYRVSRVAVDDGGRRLRAEAAAATGGASSTGDVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPADVLHDAPPASSAPLQGSRVLSAAGSDLVLTVHASPFRFTVSRRSTGDILFDTAPGLVFRDKYLEVTSALPAGRASLYGLGEHTKSSFRLRHNDSFTLWNADIGASYVDVNLYGSHPFYMDVRPPGTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIGRPAPMPYWSFGFHQCRYGYLNVSDLERVVAGYAKARIPLEVMWTDIDYMDGFKDFTLDRVNFTAAELRPFVDRLHRNAQKYVLILDPGIRIDATYGTFVRGMQQDIFLKRNGTNFVGNVWPGDVYFPDFMHPRAAEFWAREISLFRRTIPVDGLWIDMNEISNFYNPEPMNALDDPPYRINNDGTGRPINNKTVPASAVHYGGVTEYDAHNLFGLLEARATHRALLRDTGRRPFVLSRSTFVGSGRYTAHWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELCGRWIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASARKALGLRYQLLPYFYTLMYEAHMTGAPIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGATTVDAYFPAGRWYSLYDYSLAVATRTGKHVTLPAPADTVNVHVAGGTILPLQQSALTTSRARRTAFHLLVALAEDGTASGDLFLDDGESPEMGGRSDWSMVRFSCEMGSDGAIKVKSEVVHNSYAQSRTLVISKVVLMGHRSPAAPKKLTVHVNSAEVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFELKVAMSY AF9235ECE7D15B44 nia7_horvu Nitrate reductase [NAD(P)H] NAR-7 protein protein horvu Hordeum vulgare (Barley) Barley MAASVEYNRQVSAHPWPTNAQPKAAFDLFSSSGGGRRRSGADSDSDDEDSVPPDWRSLYSPRLDVEPSVKDPRDEATSDAWVKRHPALVRLTGKHPFNSEPPLPRLMSHGFITPVPLHYVRNHGAVPKADWSTWTVEVTGLVKRPVKFTMEELVTGFQAVEFPVTLVCAGNRRKEQNMVRQSSGFNWGPGAISTTVWRGVRLRDVLRRCGVMGAGAASNVCFEGAEDLPGGGGCKYGTSLRRSVAMDPARDVILAYMQNGEPLAPDHGFPVRVIVPGFIGGRMVKWLKRIVVACNESESYYHYRDNRVLPSHVDAELANAEAWWYKPECMINELNINSVITTPGHDEVLPINALTTQKPYTMKGYAYSGGGRKVTRVEVTLDGGETWQVCDLEHPERPTKYGKYWCWCFWSVEVEVLELLGAKEMAVRAWDEALNTQPERLIWNLMGMMNNCWFRVKINVCRPHKGEIGLVFDHPTQPGNQSGGWMARQKHIETSETTQGTLKRSTSTPFMSTASAQFTMSEVRRHASKDSAWIVVHGHVYDCTAFLKDHPGGADSILINAGSDCTEEFDAIHSAKARGLLEMYRVGELIVTGNDYSPQSSNADLAAIVEAPAVVVPRLPASAVALANPREKVRCRLVDKKSMSHNVRLFRFALPSPDQKLGLPVGKHVYVCASTGGKLCMRAYTPTSSVEEVGHVELLIKIYFKDEDPKFPAGGLMSQYLDALPLGAPVDIKGPVGHIEYAGRGAFTVGGERRFARRLAMVAGGTGITPVYQVIQAVLRDQPDDTTEMHLVYANRTEDDMLLREEIDRWAAANPARLKVWYVVSKVGRPEDAWEYGVGRVDEQVLREHLPLGGDGETLALVCGPPAMLECTVRPGLEKMGYDLDKDCLVF AA47EC52FC1EFD13 q42846_horvu Lipoxygenase LoxB protein protein horvu Hordeum vulgare (Barley) Barley MLLHGLVDRLTGKNKEAWKEGKIRGTAVLVKSDVLDLGDFHASLLDGVHKILGKDDGVSFRLVSATAPDPQNGSRGKVGKPAHLEEMVVTMKSTAAGESVFKVTFEWDDSQGVPGAVIVRNTYRSEYLLKTLTLHGVPGKGTVVFVANSWIYPNVDRLFFANDTYLPSKMPALLVQYRQDELNNLRGDDTTGEYKEADRVYRYDYYNDLGEPDNDNPRPVLGGTQELPYPRRCRTGRPPTETDPRSESRIPKYKIKEALNIYVPRDERFGHLKLSDFLGYSLKAITEAILPITRTYVDSTPKEFDSFQDIYNLYDGLLKVPDNQHLKELKNKSLQFIKKSLAVAGDDSKLALPHVIKSDQYAWRSDEEFAREMLAAVDPVCIRRLTKFPVKKYLDPSVYGDQSSTITEDQIQLNLEDGLTVRQAMDKKRLFILDHHDNFMPFLDRINKLEGNYIYASRTLLFLKADGTLKPLAIELSQPHPDGIQHGAKSTVYLPADINSGVDGQIWQLAKAYASVDDSAWHQLISHWLNTHAVIEPFVIATNRQLSVVHPVHKLLSPHYRDTLNINALARTTLINAGGVFELTVFPGQYALEMSAVVYKNWKLTEQGLPDDLVKRGMAVPDESSPYGIRLLIKDYPYAVDGLVIWWAIERWVNEYLAIYYPNDGVLRADKELEEWWKEVREVGHGDLKDADWWPKMVTVQELAKTCTTIIWVASALHAAVNFGQYPYAGYLPNRPTVSRRPMPKEGDEEYEQLKEGGEAADMVFIHTITSQFQTILGITLIEILSKHSSDEVYLGQRDTPEWTSDAKALEAFKRFGSRLVEIEKRILDMNKDPALKNRNGPVKMPYMLLYPNTSDANGEKALGLTAMGIPNSVSI 5EF5B57FF641B56B q70yj0_horvu Mitogen-activated protein kinase protein protein horvu Hordeum vulgare (Barley) Barley MGGGNGIVDGFRRLFHRRTPSGSVLGSSNQSSAGEDSSELEAVEDLDLVGLRPIRVPKRKMPLPVESHKKNIMEKEFFTEYGEASQYQIQEVVGKGSYGVVAAAIDTRTGERVAIKKINDVFEHVSDATRILREVKLLRLLRHPDVVEIKHIMLPPSRREFQDIYVVFELMESDLHQVIRANDDLTAEHYQFFLYQLLRALKYIHGANVFHRDLKPKNILANADCKLKICDFGLARVSFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIEIWSIGCIFAELLTGRPLFPGKNVVHQLDIITDLLGTPSSETLSRIRNEKARRYLRYMRKKHPVPLTQKFPNADPLAVRLLGRLLAFDPKDRPSAEEALADPYFASLANVEREPSRHPISKLEFEFERRKVTKDDVRELIYREILEYRPQMLEEYMKGGDQISFLYPSGVDRFKRQFAHLEEHYSKGERGSPLQRKHASLPRQRVGASNDSNNEQHISDQEMSAEPDAHGAVSPQKPQDAPGVGQNGLSPTSLSSRTYLKSASISASKCVVVNPNKQPEYDDAISEETEGAVDGLSEKVSKMHA 92D1ECB2A2629BFE ssg1_horvu Granule-bound starch synthase 1, chloroplastic/amyloplastic WAXY 259I16.5 Granule-bound starch synthase I protein protein horvu Hordeum vulgare (Barley) Barley MAALATSQLATSGTVLGVTDRFRRPGFQGLRPRNPADAALGMRTIGASAAPKQSRKAHRGSRRCLSVVVSATGSGMNLVFVGAEMAPWSKTGGLGDVLGGLPPAMAANGHRVMVVSPRYDQYKDAWDTSVISEIKVADEYERVRFFHCYKRGVDRVFIDHPWFLEKVRGKTKEKIYGPDAGTDYEDNQQRFSLLCQAALEAPRILNLNNNPYFSGPYGEDVVFVCNDWHTGLLACYLKSNYQSNGIYRTAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCELDNIMRLTGITGIVNGMDVSEWDPTKDKFLAVNYDITTALEAKALNKEALQAEVGLPVDRKVPLVAFIGRLEEQKGPDVMIAAIPEILKEEDVQIILLGTGKKKFEKLLKSMEEKFPGKVRAVVRFNAPLAHQMMAGADLLAVTSRFEPCGLIQLQGMRYGTPCVCASTGGLVDTIVEGKTGFHMGRLSVDCNVVEPADVKKVATTLKRAVKVVGTPAYQEMVKNCMIQDLSWKGPAKNWEDVLLELGVEGSEPGIVGEEIAPLAMENVAAP 0B0B3DE6A8217934 q6bct3_horvu 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase HvMS protein protein horvu Hordeum vulgare (Barley) Barley MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMAGAGIKYIPSNTFAYYDQVLDTTAMLGAVPDRYSWTGGEINLSTYFSMARGNATVPAMEMTKWFDTNYHFIVPELGPDTKFSYSSHKAINEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVEKSFSPLSLLSSILPVYKEVIAELKAAGASWIQFDEPTLVKDLESHQLAAFSAAYTELESTLSGLNVLVETYFADVPAESYKTLTSLSSVTAYGFDLERGTKTLELVKSGFPAGKYLFAGVVDGRNIWADDLAASLATLQSLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDDEIKSWLAFAAQKVVEVNALAKALAGQKDEAYFAANAAALASRRSSPRVTNEEVQKAATALKGSDHPRATTVSARLDAQQKKLNLPVLPTTTIGSFPQTVELRRVRREYKAKKISEEEYTNAIKEEISKVVKIQEELDIDVLVHGEPERNDMVEYFGEQLSGFTFTANGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSKMAQSMTARPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKKEVEDLEAGGIQVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIQSIINMDADVITIENSRSDEKLLSVFREGVVYGAGIGPGVYDIHSPRIPSKEEIADRVNKMLAVLDTNILWVNPDCGLKTRKYAEVKPALTNMVEAAKQIRAELAKAQ 67577F655DAC446A q7dmk2_horvu protein protein horvu Hordeum vulgare (Barley) Barley SSEAAGPDGTLVRRYTTGPLCRWLTKDRGDGTLSPFAVFVVDPDHLFPWHHIAEAVTAGGPSAFERTQKWPYYEYMGKNQRLGTLFDNAMAQHSVILVTKMLERFKGFDGVQRLVDVGGGTGSTLGMITSKYKHMTGINYDLPHVIAQGLPLPGVEHVAGDMYESIPTGDAVLLQWITLMLNDDEFVKILSNCHNALPKDGKVIVVDGILPENPDSSLTARDAFTLDIIMFVLFKGAKQRTEKEFARLAKQAGFTGGIKKTYIFFNFYALEFTK E272DD57631FA1EC matk_horvu Maturase K matK ycf14 Intron maturase protein protein horvu Hordeum vulgare (Barley) Barley MEKFEGYSEKQKSRQQYFVYPLLFQEYIYAFAHDYGLNGSEPVEIVSWNNKKFSSLLVKRLIIRMYQQNFLDNSVNHPNQDRLLDYKIFFYSEFYSQILSEGFAIVVEIPFSLRELSCPKEKEIPKFQNLRSIHSIFPFLEDKFLHLDYLSHIEIPYPIHLEILVQLLQYRIQDVPSLHLLRFFLNYYSNWNSFITSMKSILFFQKENKRLVKFLYNSYVSEYEFFLLFLRKQSSCLPLAYSGTFLERIHFSRKMEHFGIMYPGFSRKTLWFFMDPLIHYVRYQGKAILASKGSFFLKKKWKCYLINFWQYYFFFWTQPRRIHINQLANSCFDFMGYLSSVPKSPLLVRNQMLENSFLIDTRMKKFDTIVPATLLIGYLSKAQFCTGSGHPISKPIWTDLSDWDILDRFGRICRNLFHYYSGSSKKRTLYRLKYILRLSCARTLARKHKSTVRTFMQRLGSAFLEEFFTEEEQVFSLMFTKTTLFSFSGSHTERIWYLDIIGINDLVNPLN 41809CFC4653080A o23812_horvu Ids1 MT1b3 protein protein horvu Hordeum vulgare (Barley) Barley MSCSCGSSCGCGSNCNCGKMYPDLEEKSGATMQVTVIVLGVGSAKVQFEEAAESGEAAHGCSCGANCKCNPCNC F86C8CC3ED58B1C6 14-3-3 1 30 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 5 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 17 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 3 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 28 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 13 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 24 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 31 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 20 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 6 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 8 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 12 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 4 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 2 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 16 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 15 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 23 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 27 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 7 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 10 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 14 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 22 unspecified role unspecified role bait bait c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecobb Escherichia coli (strain B / BL21) 29 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 19 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 26 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 11 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 21 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 25 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 18 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley 9 unspecified role unspecified role prey prey horvu Hordeum vulgare (Barley) Barley association association The participant EBI-906214(Q84NG8) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later.