IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination. Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination. Eukaryotic Cell (1535-9778) 2005 imex curation Schimanski B., Nguyen TN., Gunzl A. In some cases the tag used was a derivative of the original TAP tag where the calcium binding peptide was replaced by the 12 amino acid epitope protein C (ProtC) for a ProC-TEV-ProtA tag. This tag is referred to as PTP in the text. An anti-ProtC matrix replaced the calmodulin affinity resin for purification of proteins. Figure 2(B). Figure 3 (B) and (D). gunzl@uchc.edu Only protein-protein interactions 9tryp Trypanosoma brucei affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Schimanski B., Nguyen TN., Gunzl A. In some cases the tag used was a derivative of the original TAP tag where the calcium binding peptide was replaced by the 12 amino acid epitope protein C (ProtC) for a ProC-TEV-ProtA tag. This tag is referred to as PTP in the text. An anti-ProtC matrix replaced the calmodulin affinity resin for purification of proteins. Figure 2(B). Figure 3 (B) and (D). gunzl@uchc.edu Accepted 2006-MAR-16 by JYOTI. Eukaryotic Cell (1535-9778) 2005 Only protein-protein interactions imex curation q387x6_tryb2 Plastid-encoded RNA polymerase subunit alpha Tb10.61.1040 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVKVYNLQRRTETLPTVRRTAPNAEELEYQSHLSRPAGAGDGEPTRRVETITFDMDNVSPPVANMFRRLMLTEVPVFAFDRILIEHNDGVVPDELLAHRIGLVPLAGPVSSMQYITDSASVGFDNLDPERVLLFELDVTAKRDVPTTSVYSGHLKWQPIAKQEELAARSEDDRVFLVHPDIVLTRLGPGQRLKLRAIAIKGIGAVHAKWTPVAACFYEMKQAKAGAEDSDGRASGRGRSRSGRGRVDANEEESNATRPRSASGNHSHEPHRETVNEVIAKRDTSSVRFTVESIGQLYAVDVFRQALILFAERIRDLAVRIRSTEPRMTGAAATAFTE 40342EB5863B53EE q4gyy6_tryb2 Tubulin beta chain TB927.1.2330 TB927.1.2350 TB927.1.2370 TB927.1.2390 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MREIVCVQAGQCGNQIGSKFWEVISDEHGVDPTGTYQGDSDLQLERINVYFDEATGGRYVPRSVLIDLEPGTMDSVRAGPYGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELIDSVLDVCCKEAESCDCLQGFQICHSLGGGTGSGMGTLLISKLREQYPDRIMMTFSIIPSPKVSDTVVEPYNTTLSVHQLVENSDESMCIDNEALYDICFRTLKLTTPTFGDLNHLVSAVVSGVTCCLRFPGQLNSDLRKLAVNLVPFPRLHFFMMGFAPLTSRGSQQYRGLSVPELTQQMFDAKNMMQAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQNKNSSYFIEWIPNNIKSSVCDIPPKGLKMAVTFIGNNTCIQEMFRRVGEQFTLMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATIEEEGEFDEEEQY 628D04DB20170A4B q57ul1_tryb2 Tb927.8.5390 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSTVNRLQCIGKLGEGTYGVVYKARELATGQIVAYKRIIVGSEDDGAPSTAIREIALLKVLRHNNIVRLYDVLFEPPKLTLIFEYCEYDLRRFMHKHDSRVRTQVKEILKQVLLGLRYMHQRSVVHRDLKPDNIFVRVDGISGSEGSEMSVSSRETEVVNTRGHWDDIDEAANCPKNANPTENPNARIASEDHGADGTVPSGSTTPTSQLVIKLGDYGMARIESIPVKKYSHDVASLWYRSPDALLGSAMYGFAVDLWSVGCIFAEMVTGAPLIRGNTDVDQLLKTFKLLGTPTPETWPSMKNCPKAVQLLKAAAELARGEPAENGKKTKNSRGVHKQHERNLPNVQKTATGTSHLSAVMRVGIDGESGCVADPRDELYHFPPELCFPSAFDEYVKASGFQGRVGVEGTDFLRRLIRYEPSQRMSVQEALSHPFVSNTVALMQRPLDVMASMLRQSLREHSVYP C9C52DC40AEA98D0 q383n7_tryb2 Tb11.01.2190 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSAPMARRDYFSMGSRCCIACGQWIPSEHDMPRSGSAAAGGLQCRRCNVVQPERPMPDFEKASEVTTAALLFTKAEIDAAWKSVKHSLEEARREDVGVAQTDNRVEEEAFCEKCGVPRKCKVFARQIRSADEGQTVFYQCTSCDSEWQLNS 3E75208B2FF13FB8 q4krh6_9tryp protein protein 9tryp Trypanosoma brucei MQQRLVLIATDFVTLYQEALSRQLLTPAALTPDAFKDLFDRINVEYMHYAGAGATQPYFEDVVENLLQLAAAYITLPPDAAPNSRAFGVYLTFFLYATQPAIETSPVKVQISLGTLQRYVEDIDSTARDNQGVITSLGCRVSDGEKRLLLALHKSGALKVMPFIDDSLYVRTLIEVHEQAGLPLLTCVAPQRSNPSPHIALEGGTCVDDDLSNQLHAYREMRRRINTESLLKRK 26D8850FD1A58276 q387i8_tryb2 DNA-directed RNA polymerase subunit beta Tb11.03.0450 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSTKNSVVGSASGSHASTAGETVTSTRSGTSSRHSRQRRQQYDYVKNPSEPMTAMERLKLVRCSIARLRYLGERHRHTSKLDTTGGDRLLSFFEGLRKRVLKLEYNPRVVAKSKVGAPKHLDYIRGHANALLGEASTIISECEQAMDRSEAVYEGLAYHQLQDTHRELENLVGLLSGAPSDMESAGSLKLLADLVSPHVAEFDAFAEQRVPEFLQELKNEGFLFSSVINDSQQPANAIGLLSVFIVDVAIGVRPGRSARLVIQHALHNLARLKSRVDKADLTLGAEEDSSNNEHHRMQSLEFEVIRGRLREAKEELERIRDKWDHDEGDIFSRAGTGVQGLQSIIDAKRVKESTVIEEAQAIHDRTVYACLPFASPQYCRIRSETYSQPCHVRVGFKFVAKGDGSVSRTKTQLIYNLMPEFLILMRQQGIAPSDQERFATLAPSKYELWLSLGELPEMVLGARCALRGVNQRLDHFRALEEQKEVGGYFVLRGGERILRNLILQRCNVPLNIERERFATVDSFFSPRAVIIRCKRPSGLTVQNYFYYATKGEVIFTFARRVVWNIPALLVLSALNTRHRTSLDIYKLLTIGLTNGRSPHAARVEALLQHHSQMPYSTLDNFLDYLAVVGLMYRRYHESSFIFRFLPQYHTSFATQHDAWYGLFMLRRHMLPHLNTAEATPDLPPTATSREIREWLSTGLQEELAAKFDALIGILRQLLVFNDGATGNQGNDVLAYQEVFTVSQVLIGAFEVSLMKTMGAVTFRMAGQLSPEIFQATMCLAETPIREAANTLEKLNQLLEYSCRNNGPDPLITLNRLLLTGNFALDREEDFYSPQTAGWAVMTEHLNFYRSFEQLMCVHRGKSIAEMRSSDVRRYPCEAYGFICMVHSPDGEDCGVLNHLSISTVLSASPRVGSSQYKILLAFIEEELKNVRNTTSLISCVDQLVGTVPVWLEGRLLGYLSPNEALSASKALTQKKALRSRGVVGVTGIVRRDSISSLNTMEVVYIPPGSKDPMGLYIFYQYGRLMRPVLQIESPSRGGDLPFPVVYLGTWEQSWRDIAAVPSDLFDGFDQLRRKYEFMEQNGFNILSFTSSTIPFFEHNCSPRNLFQCGLSKQTAGTQLQTQAWRRDAKLFRMYYPQRYISRTLPMDYFGLDDVSLGVNAVVAILAYTGYDLDDAMILNSTAKQFGMLNAGVTVAKIVKASGKGDSNDVFVFHNCLPDGQPFTPELDRHGLPKKRAVPNATAYFSFDTDHKYPSLRDSSAVYCCAKRFEYIDPLTNEKTYEYTRHQITKWRHFDKGEDAWVHSVIPLTYDGPDPTSVLVLFRIPRPPVIGDKFSSRHGQKGTLPLDIRSHDLPFSTRDGITPDIIINPHAFPSRMTVGMVLEMMGAKLGCIQGRLCDHSAWSVIDEQPRSAKTIGDALEALGYNRYGREKLIDGITGEEIEAEVLVGISGYQRLRHMVSDKWQARARTDSHTHRAVTKTGQPVKGRKRHGGVRVGEMERDALLSHGVAEVVIDRLLHVSDKTKAFICVECGSMLSIYERHATEYSTWKTCKFCSAGLHETDDTIAFVEIPQVLRLWATELTSVGIRVVLKTSETSL 752E09C31486729B q57u75_tryb2 Tb927.5.3910 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MRRNNRTRCTIDDVNGMLARNAQLRNALQERYKQLKMRYEQLAALRAALSPSRGVTLRKLGVRQETADGAEEVRFLDDYTTGGVGNPPFRDAGIYSAKNIVCYAPVPPTRDELRWKGVTLAFPQLAYVHSLAMLPETANSFSKALQWSREEDSALREQVHAYKGARCGPSFWKALGAPGQSRFEVASHYIRLQQLGLIEPGKNDKTMMWIPEEQRDIALREAVWRHLGDEGGIMAAYVEIISVAARKCVYLSEVTCNESLVFPPYVWVKRTTFNWLTSLLIQKAKAPLVRCEGEFSDDTPLCMHFKSDISLKLSAEDMMACLLAFKGEVFGEVGGLRFIERAFLPKNRTYALKATDFMAKREGKKHKLDEEV F3AB148B0615B3F6 q387v6_tryb2 Tb10.61.0780 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MNGRYARQSGCFRGKRPREITTQKLFNRAIEHFQSIYNDSVERHERETFASRAVFEKQCGVDREHFNRAHGSLAKHGKGKDFALVLEQFENEEAKLQREHEEELQRVYPLAFLELDTAAVLSLTLIPRLKSKLDLLERQLTQRRKFTNQHQGDAAARGSHSRDVDPPVDAKESDDKNGADGVPSELNDAKTGIEEDDVGTLCVGRGAYITGDEPSEVEESDSSALVQVLDTSVEDGKVGTGTGGGFRNHVHRLVSRGLGNILSIPTDVMRAGAADFARIGLLLNTESRRQRRDERRKFLRSHMEVCGPEGKTIGKWELKRLLQEGKEDKLKQEEIFAALEAAKTMPEFTGSDMNANIVESMENDGAPRKTAGASTASRETGDTTASETVVKRHTKIVLRGNGSRRDDGVPFTRKDFEWTSLGLDDESIGEGSRMEKLAESYMWPKRTEDRIPHILCGEVIPTYFRVRQYQLDTSSPTTATVQMSATGEDGCPSTLLDDLAEATQLDPVREGMSNGEKGVVTKKSPLVFRIPFRAKSVWLKVDTLGDFIASQVKFFGAVTSREI 7B6450F6BF5D59F2 q38bb7_tryb2 Tb10.70.2360 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MYRESLLGVALGATLAELESDCLLTEGQKQQLWEIFDIAMDRTLAEAPVRSQVRVRTSAPSLVGHATLGGESGLSSIDAPTLSTRPAEMEFPLPPSEVTHTVATTPCDVLDEEVLYPVYRVKDGMWTILLKDPILELTDATGSTETIRLDYLKVYLKEVVSRSATSVSASSRKKRKRSRP 7D17AD2821BF4FF5 q4gyy5_tryb2 Tubulin alpha chain TB927.1.2340 TB927.1.2360 TB927.1.2380 TB927.1.2400 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MREAICIHIGQAGCQVGNACWELFCLEHGIQPDGAMPSDKTIGVEDDAFNTFFSETGAGKHVPRAVFLDLEPTVVDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVYHAVGGGTGSGLGALLLERLSVDYGKKSKLGYTVYPSPQVSTAVVEPYNSVLSTHSLLEHTDVAAMLDNEAIYDLTRRNLDIERPTYTNLNRLIGQVVSSLTASLRFDGALNVDLTEFQTNLVPYPRIHFVLTSYAPVISAEKAYHEQLSVSEISNAVFEPASMMTKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWSPTGFKCGINYQPPTVVPGGDLAKVQRAVCMIANSTAIAEVFARIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESADMDGEEDVEEY 68BF6E3EFED70D72 q387r9_tryb2 TATA box-binding protein-like 1 TBP-like factor Tb10.61.0330 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDNDFTGNFDMDENDFFIDEDEGDMDFLQRAAGASGVYVDAAPKVVMENNEQMKGECSDRVAEVNIQELFPNADPATVPLVVGIIAQAKLGVGVDLRALSCATRNVEFTPRVRCPAATLRLHEPNAVVQVRTSGLLGIIGSTSVGEARQATELAARIIRKALNLNFSSVQFRVRSLMARFDLCHPIRLDELAKHEGVFCSYEPDRFSGCIVRLSGSSRGNQWQVCCTVFVTGKVIVLGARSREELLDAFYTVLPIIAQYAKR FB8333746411E73A q388r6_tryb2 Tb10.389.0120 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDSVKSFKMIRLYRAFNTVIQMAIDRGFVVSHPSDVANAIRDSRLYDITDGLNYQWFTQRCSSYLQRHRQTTAEEEEEAKQEGRPGKDPSKSKRTKALRDASQDFLYSSLELVCKGSSSRIGFGGGAISCKTEDEVDGRGPTAEPSSRKKNETILCAFFVQSLGVDVLNNLREVAQRRNASSMIVVVGSKVRGGRRSIREAGGGLHPGTTGQVIRIQVFEEDELAYNISRHQSVPKHVPMTDVEVRAFLEQRKLMITQLPRILNDDPMVEYLDLPRGSIIRIERRTDEGSTYEMYRHVI 6D8B18E1150051E7 q38e05_tryb2 Tb09.211.1510 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPPKQLVDPFSLQVGNKKETTLANVAAHTAVTLTDIRRRNPQLDVYKISDKLPAGTIVHLPQTLSERGNPYGCFFTIGEAEDAMRKLEARLDRLLEEEGPNLPELPTRRVLSKMERIVTQVKEEGERQLARVREEYSECERRPALLQFLVDLEPVKRLREQLDEEYDGRKLRLKVMDEGNSELFRQAEPRYPNKGIAASLRHNVIDTSEDFCLTEHSHRWEFTFFTPGSTTEPQETWAVLSCQSLGTLLDTFVCRNCIPVNISKNAFFFIGGTFYVDNRHAGEGGEDYEDLTAPIRHFDPCGEGASTEGETRQKNIAFGNCPVKYVSQTTFGDLNLRLGEYGVMRHLGWCNHYFYLSSVTSLRGFDRDDHTRAAYPQRVMKTPTRVVRCRLCRSHPATVVCYNDEISPESPCPYCVPCFELLHATDEGEVEEGKFFAIRLPQGKYYTA 0794FB7766906A6E snap50 1 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait n terminal ProtC-TEV-ProtA tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association snap50-1 1 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association snap50-2 1 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait c terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association rpa1 1 11 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association tfall-1 1 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey association association