IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. Arch. Biochem. Biophys. (0003-9861) 2005 imex curation Bonsegna S., Slocombe SP., De Bellis L., Baker A. a.baker@leeds.ac.uk Whole plant arabidopsis cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Bonsegna S., Slocombe SP., De Bellis L., Baker A. a.baker@leeds.ac.uk Accepted 2006-OCT-4 by ORCHARD. Whole plant arabidopsis cDNA library Arch. Biochem. Biophys. (0003-9861) 2005 Only protein-protein interactions imex curation AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. Arch. Biochem. Biophys. (0003-9861) 2005 imex curation Bonsegna S., Slocombe SP., De Bellis L., Baker A. a.baker@leeds.ac.uk Whole plant arabidopsis cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bonsegna S., Slocombe SP., De Bellis L., Baker A. a.baker@leeds.ac.uk Accepted 2006-OCT-4 by ORCHARD. Whole plant arabidopsis cDNA library Arch. Biochem. Biophys. (0003-9861) 2005 Only protein-protein interactions imex curation AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5. Arch. Biochem. Biophys. (0003-9861) 2005 imex curation Bonsegna S., Slocombe SP., De Bellis L., Baker A. a.baker@leeds.ac.uk Whole plant arabidopsis cDNA library Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Bonsegna S., Slocombe SP., De Bellis L., Baker A. a.baker@leeds.ac.uk Accepted 2006-OCT-4 by ORCHARD. Whole plant arabidopsis cDNA library Arch. Biochem. Biophys. (0003-9861) 2005 Only protein-protein interactions imex curation lacs7_arath Long chain acyl-CoA synthetase 7, peroxisomal LACS7 F15A18.60 At5g27600 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEFASPEQRRLETIRSHIDTSPTNDQSSSLFLNATASSASPFFKEDSYSVVLPEKLDTGKWNVYRSKRSPTKLVSRFPDHPEIGTLHDNFVHAVETYAENKYLGTRVRSDGTIGEYSWMTYGEAASERQAIGSGLLFHGVNQGDCVGLYFINRPEWLVVDHACAAYSFVSVPLYDTLGPDAVKFVVNHANLQAIFCVPQTLNILLSFLAEIPSIRLIVVVGGADEHLPSLPRGTGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGSSVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFCSVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEKLGGRVRFMGSGASPLSPDVMDFLRICFGCSVREGYGMTETSCVISAMDDGDNLSGHVGSPNPACEVKLVDVPEMNYTSDDQPYPRGEICVRGPIIFKGYYKDEEQTREILDGDGWLHTGDIGLWLPGGRLKIIDRKKNIFKLAQGEYIAPEKIENVYTKCRFVSQCFIHGDSFNSSLVAIVSVDPEVMKDWAASEGIKYEHLGQLCNDPRVRKTVLAEMDDLGREAQLRGFEFAKAVTLVPEPFTLENGLLTPTFKIKRPQAKAYFAEAISKMYAEIAASNPIPSKL EAD95B9F50DA75DB pex5_arath Peroxisome biogenesis protein 5 At5g56290 PEX5 Peroxin-5 Peroxisomal targeting signal type 1 receptor Pex5p MXK23.3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAMRDLVNGGAACAVPGSSSSSNPLGALTNALLGSSSKTQERLKEIPNANRSGPRPQFYSEDQQIRSLPGSELDQPLLQPGAQGSEFFRGFRSVDQNGLGAAWDEVQQGGPMPPMGPMFEPVQPTFEGPPQRVLSNFLHSFVESSRGGIPFRPAPVPVLGLSQSDKQCIRDRSSIMARHFFADRGEEFINSQVNALLSSLDIDDGIQARGHVPGRFRELDDYWNESQAVVKPNLHPADNWAAEFNQHGMDHGGPDSWVQSFEQQHGVNGWATEFEQGQSQLMSSQMRSMDMQNIAAMEQTRKLAHTLSQDGNPKFQNSRFLQFVSKMSRGELIIDENQVKQASAPGEWATEYEQQYLGPPSWADQFANEKLSHGPEQWADEFASGRGQQETAEDQWVNEFSKLNVDDWIDEFAEGPVGDSSADAWANAYDEFLNEKNAGKQTSGVYVFSDMNPYVGHPEPMKEGQELFRKGLLSEAALALEAEVMKNPENAEGWRLLGVTHAENDDDQQAIAAMMRAQEADPTNLEVLLALGVSHTNELEQATALKYLYGWLRNHPKYGAIAPPELADSLYHADIARLFNEASQLNPEDADVHIVLGVLYNLSREFDRAITSFQTALQLKPNDYSLWNKLGATQANSVQSADAISAYQQALDLKPNYVRAWANMGISYANQGMYKESIPYYVRALAMNPKADNAWQYLRLSLSCASRQDMIEACESRNLDLLQKEFPL 12EF434E8C06DDFB lac7-pex5 1 5 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-993892]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 793-793, sequence length 728 [EBI-993892]330-793 4 unspecified role unspecified role bait bait physical association physical association Figure 2A lacs7-pex5-1 2 5 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-994025]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 793-793, sequence length 728 [EBI-994025]330-793 4 unspecified role unspecified role bait bait physical association physical association Figure 2B and Figure 3B Interaction between full length LACS7 and PEX5 proteins was judged to be stronger than interaction between full length LACS7 and truncated PEX5 (330-793) by beta-galasctosidase assay. pex5-lacs7 3 5 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-994056]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 793-793, sequence length 728 [EBI-994056]330-793 region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Figure 4 lacs7-pex5 3 5 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-994089]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 793-793, sequence length 728 [EBI-994089]330-793 region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Figure 4