IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. J. Exp. Med. (0022-1007) 2005 Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu 01-DEC-2006: Contacted by JYOTI. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu Accepted 2006-NOV-21 by DAVET. 01-DEC-2006: Contacted by JYOTI. J. Exp. Med. (0022-1007) 2005 Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. J. Exp. Med. (0022-1007) 2005 Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu 01-DEC-2006: Contacted by JYOTI. human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu Accepted 2006-NOV-21 by DAVET. 01-DEC-2006: Contacted by JYOTI. J. Exp. Med. (0022-1007) 2005 Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. J. Exp. Med. (0022-1007) 2005 Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu 01-DEC-2006: Contacted by JYOTI. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu Accepted 2006-NOV-21 by DAVET. 01-DEC-2006: Contacted by JYOTI. J. Exp. Med. (0022-1007) 2005 Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. J. Exp. Med. (0022-1007) 2005 Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu 01-DEC-2006: Contacted by JYOTI. human-calu_3 Homo sapiens lung adenocarcinoma calu_3 male caucasian lung adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu Accepted 2006-NOV-21 by DAVET. 01-DEC-2006: Contacted by JYOTI. J. Exp. Med. (0022-1007) 2005 Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. Lysophosphatidic acid inhibits cholera toxin-induced secretory diarrhea through CFTR-dependent protein interactions. J. Exp. Med. (0022-1007) 2005 Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu 01-DEC-2006: Contacted by JYOTI. crisp-kidney Cricetidae sp. kidney kidney [Renal] crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Li C., Dandridge KS., Di A., Marrs KL., Harris EL., Roy K., Jackson JS., Makarova NV., Fujiwara Y., Farrar PL., Nelson DJ., Tigyi GJ., Naren AP. Macromolecular complex assembly studied. Author correspondence LPA2 and CFTR was of human origin. Cell lysates from HT29-CL19A were used. anaren@utmem.edu Accepted 2006-NOV-21 by DAVET. 01-DEC-2006: Contacted by JYOTI. J. Exp. Med. (0022-1007) 2005 cftr_human Cystic fibrosis transmembrane conductance regulator CFTR ABCC7 cAMP-dependent chloride channel ATP-binding cassette sub-family C member 7 Channel conductance-controlling ATPase protein protein human Homo sapiens Human MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKNPKLINALRRCFFWRFMFYGIFLYLGEVTKAVQPLLLGRIIASYDPDNKEERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAGLGRMMMKYRDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAYVRYFNSSAFFFSGFFVVFLSVLPYALIKGIILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTETKKQSFKQTGEFGEKRKNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGPTLQARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVSLAPQANLTELDIYSRRLSQETGLEISEEINEEDLKECFFDDMESIPAVTTWNTYLRYITVHKSLIFVLIWCLVIFLAEVAASLVVLWLLGNTPLQDKGNSTHSRNNSYAVIITSTSSYYVFYIYVGVADTLLAMGFFRGLPLVHTLITVSKILHHKMLHSVLQAPMSTLNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRVFKFIDMPTEGKPTKSTKPYKNGQLSKVMIIENSHVKKDDIWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLLNERSLFRQAISPSDRVKLFPHRNSSKCKSKPQIAALKEETEEEVQDTRL 8D082AA2E768C065 sl9a2_human Sodium/hydrogen exchanger 2 SLC9A2 NHE2 Na(+)/H(+) exchanger 2 Solute carrier family 9 member 2 protein protein human Homo sapiens Human MEPLGNWRSLRAPLPPMLLLLLLQVAGPVGALAETLLNAPRAMGTSSSPPSPASVVAPGTTLFEESRLPVFTLDYPHVQIPFEITLWILLASLAKIGFHLYHKLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTDVFFLYLLPPIVLDAGYFMPTRPFFENIGTIFWYAVVGTLWNSIGIGVSLFGICQIEAFGLSDITLLQNLLFGSLISAVDPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIETIDVFAGIANFFVVGIGGVLIGIFLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIMAITACAMTMNKYVEENVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCLMWRALGVFVLTQVINRFRTIPLTFKDQFIIAYGGLRGAICFALVFLLPAAVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKRSNKKQQAVSEEIYCRLFDHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLEIKHAIEMAETGMISTVPTFASLNDCREEKIRKVTSSETDEIRELLSRNLYQIRQRTLSYNRHSLTADTSERQAKEILIRRRHSLRESIRKDSSLNREHRASTSTSRYLSLPKNTKLPEKLQKRRTISIADGNSSDSDADAGTTVLNLQPRARRFLPEQFSKKSPQSYKMEWKNEVDVDSGRDMPSTPPTPHSREKGTQTSGLLQQPLLSKDQSGSEREDSLTEGIPPKPPPRLVWRASEPGSRKARFGSEKP 17EE177DC3830D0A nhrf2_human Na(+)/H(+) exchange regulatory cofactor NHE-RF2 SLC9A3R2 NHERF2 Tyrosine kinase activator protein 1 SRY-interacting protein 1 Solute carrier family 9 isoform A3 regulatory factor 2 NHE3 kinase A regulatory protein E3KARP Sodium-hydrogen exchanger regulatory factor 2 protein protein human Homo sapiens Human MAAPEPLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHHQVVQRIKAVEGQTRLLVVDQETDEELRRRQLTCTEEMAQRGLPPAHDPWEPKPDWAHTGSHSSEAGKKDVSGPLRELRPRLCHLRKGPQGYGFNLHSDKSRPGQYIRSVDPGSPAARSGLRAQDRLIEVNGQNVEGLRHAEVVASIKAREDEARLLVVDPETDEHFKRLRVTPTEEHVEGPLPSPVTNGTSPAQLNGGSACSSRSDLPGSDKDTEDGSAWKQDPFQESGLHLSPTAAEAKEKARAMRVNKRAPQMDWNRKREIFSNF 4F5D341590D22ED7 lpar2_human Lysophosphatidic acid receptor 2 EDG4 Lysophosphatidic acid receptor Edg-4 LPAR2 LPA2 protein protein human Homo sapiens Human MVIMGQCYYNETIGFFYNNSGKELSSHWRPKDVVVVALGLTVSVLVLLTNLLVIAAIASNRRFHQPIYYLLGNLAAADLFAGVAYLFLMFHTGPRTARLSLEGWFLRQGLLDTSLTASVATLLAIAVERHRSVMAVQLHSRLPRGRVVMLIVGVWVAALGLGLLPAHSWHCLCALDRCSRMAPLLSRSYLAVWALSSLLVFLLMVAVYTRIFFYVRRRVQRMAEHVSCHPRYRETTLSLVKTVVIILGAFVVCWTPGQVVLLLDGLGCESCNVLAVEKYFLLLAEANSLVNAAVYSCRDAEMRRTFRRLLCCACLRQSTRESVHYTSSAQGGASTRIMLPENGHPLMDSTL DE225B0B3E9351B8 cftr-nhe2 2 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association 6E LPA2 forms a macromolecular complex with CFTR mediated through NHERF2. cftr-nhe2-1 4 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait association association 6E LPA2 forms a macromolecular complex with CFTR mediated through NHERF2 cftr-edg4 5 6 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6D cftr-slc9a3r2 1 8 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait c terminal tail sufficient to bind sufficient binding region sufficient to bind c-term range C-terminal range c-term range c-term range C-terminal range c-term range region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 6B Co-ordinates of C-terminal of P13569 not given by authors cftr-slc9a3r2-1 1 6 unspecified role unspecified role bait bait c terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal tail sufficient to bind sufficient binding region sufficient to bind c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9HBW0:p.Leu351Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:21:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu351ala' Tue Jun 30 18:09:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu351Ala' c terminal deletion required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) association association 6B A macromolecular complex was formed between maltose binding protein (MBP)-C�CFTR, glutathione S-transferase (GST)-NHERF2, and LPA2 (Fig. 6 B). A macromolecular complex was formed between maltose binding protein MBP-CFTR, glutathione S-transferase GST-NHERF2, the complex was immobilised on amylose beads and this was then used to pull down LPA2 from lysates. cftr-slc9a3r2-2 3 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell physical association physical association S2B Flag-tagged LPA2 binds GST-NHERF2 with the highest affinity. cftr-slc9a3r2-3 3 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal tail sufficient to bind sufficient binding region sufficient to bind c-term range C-terminal range c-term range c-term range C-terminal range c-term range mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell physical association physical association S2B GST-NHEFR2 binds directly to maltose-binding protein (MBP)-C-LPA2.