UniProt UniProt http://www.uniprot.org Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. Nat. Cell Biol. (1465-7392) 2005 imex curation Jenny A., Reynolds-Kenneally J., Das G., Burnett M., Mlodzik M. marek.mlodzik@mssm.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Jenny A., Reynolds-Kenneally J., Das G., Burnett M., Mlodzik M. marek.mlodzik@mssm.edu Accepted 2006-NOV-23 by DAVET. 23-MAY-2007: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. Nat. Cell Biol. (1465-7392) 2005 imex curation Jenny A., Reynolds-Kenneally J., Das G., Burnett M., Mlodzik M. marek.mlodzik@mssm.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Jenny A., Reynolds-Kenneally J., Das G., Burnett M., Mlodzik M. marek.mlodzik@mssm.edu Accepted 2006-NOV-23 by DAVET. 23-MAY-2007: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. Diego and Prickle regulate Frizzled planar cell polarity signalling by competing for Dishevelled binding. Nat. Cell Biol. (1465-7392) 2005 imex curation Jenny A., Reynolds-Kenneally J., Das G., Burnett M., Mlodzik M. marek.mlodzik@mssm.edu 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jenny A., Reynolds-Kenneally J., Das G., Burnett M., Mlodzik M. marek.mlodzik@mssm.edu Accepted 2006-NOV-23 by DAVET. 23-MAY-2007: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2005 Only protein-protein interactions imex curation q7juf2_drome dgo Dgo Dmel\CG12342 EP(2)2619 EP2619 CG12342 Dmel_CG12342 DGO protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQHGSSRIPKQEALPNAISGPDSSNWTSLHEAVAAGDDRRVEGLLFSNADRLARESVQGNTPLHEAASRGFSRCVKLICTPPTPPASSGVGKAAKGQKDKDKDKNKGRVKVAHQTIEALHNSALGIANNEGLSALHLAAQNGHNQSSRELLLAGADPDVRNNYGDTPLHTACRYGHAGVSRILLSALCDPNKTNLNGDTALHITCAMGRRKLTRILLEADARLGIKNAQGDCPMHIAIRKNYREIIEILNTPKKIRNRKEKPKEGGSRSDKDRDRERDVVDKGINWSPYGCHYFPDPRAFPSPKLETLPKEPLKSGEQYFLDLAGHIHKGPVSVGNTCYCGPFFRHIENKLNCNRKSLKKYVHKTKERLGHKVQALAIKTNDQIEQLTRTMIEDRLRCESKRQHLSEFLRRGEPMRSTFDQQNKSSRIERTMSRCRSLELLENNHEGGRLTNSRSVDVLEDQAVEAIVHRSAEVQVDSDSDDDSNEARDEEEAEVGDKDQVQEEAEPEEHSKLDELKLDFLKVSERLGVLLEKTTLIMERDSEQESKRQLSSLSPPYNPHPRAESAQLREDKESVNSPNFDEYANVIRRYPNSSETSNPSQNSNSWDWEASPTGSNPHPDYNKYYQRIGRGENMLNSVIKALRKDASFADLGKEETAVNETAEGMGGDKLLYKEQASEAAGISNLMKRQPLCLEDNYPVMSNLFYSNPIMEYAEETEVRQNGNESMSKVEIRNSEIKCLKKPNAGQVRDMVAQLQNTIDSNRQDSQTAHVIAARSHSRLNNNFHHLEGTSSNPSPTYSIPHRSSAQFGLPERHIPKDAYFHELPHRPPIPQTPQRRLHSGYVPNASLYRSEDLYVGRIPHPNAPPRQVDYVDAVAYRPLQSPTFQAIVPPHVNQRPRNWPHPNLPSNEIDLDELSAVGLYNNVSSLV DIP protein Q7JUF2 original sequence version: 52 7743AE0772753E27 pric1_drome Protein prickle Protein spiny legs pk CG11084 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSLSTGGGAGGSSGGPGGADAAAAPAAGQATVTATGNMEPAMVPRTANLLACKQWWRVCFLYGDQQKYYRQLYSKAAAQRLADANQEPDNARDREYDTVDCDLIAGQLDAVEDADDGIDLGDHSSTPKGGATTAGRPLFPHSSSPRRSKKLLRSLRAHVRGEKLPKNDTTTANESSEVTQRNARVTVLDDPFLFGIDADHLGDLVVRGKRYSTLDATENMARFYAEQEATAQVLEIIEQEEESPEQEAPKPALPPKQKQQRPVPPLPPPPANRVTQDQGTQPAAPQVPLQPLTAGDLQFLNLSLRQRSLPRSMKPFKDAHDISFTFNELDTSAEPEVATGAAQQESNEPISRTPLTQISYLQKIPTLPRHFSPSGQGLATPPALGSGGMGLPSSSSASALYAAQAAAGILPTSPLPLQRHQQYLPPHHQQHPGAGMGPGPGSGAAAGPPLGPQYSPGCSANPKYSNAQLPPPPHHHHQLSPALSTPSPPSLLHHPAGGTSSASAHAPFLGGPHMDMQRQSHSDDDSGCALEEYTWVPPGLRPDQVRLYFSQIPDDKVPYVNSPGEQYRVRQLLHQLPPHDNEVRYCHSLTDEERKELRLFSTQRKRDALGRGNVRQLMSARPCDGCDDLISTGDIAVFATRLGPNASWHPACFACSVCRELLVDLIYFHRDGRMYCGRHHAETLKPRCSACDEIILADECTEAEGRAWHMNHFACHECDKQLGGQRYIMREGKPYCLHCFDAMFAEYCDYCGEAIGVDQGQMSHDGQHWHATDECFSCNTCRCSLLGRAFLPRRGAIYCSIACSKGEPPTPSDSSGTGMYTTPTPPTQRVRPHPQAPLPARIPSSHASSSPPMSPQQQQQHQATFNQAMYQMQSQQMEAAGGLVDQSKSYAASDSDAGVVKDLEHGGHMGGGDLTDFSGGRASSTSQNLSPLNSPGDFQPHFLPKPMELQRDGVYNFNEMSSNLDAAWSAKPTNSYHLQRQLLENPHTASMPELAGKLVAPPAHMQHLSQLHAVSSHQFQQHEYADILHPPPPPPGEIPELPTPNLSVASTALPPELMGSPTHSAGDRSLNTPMSTQSASHAPPHPVSILSGASSSSPMSGEPAKKKGVRFEGIPDTLPRSRSYSGNGAGTSGGGERERDRDKDKEGGGRHGHGHSSRRRRRRKSSSSSSHHRSGSGHRSHSTTRADTYAPAQPLSSSYQGPPSVLQAANLVHESPSRQQREREREREREESEESDVCSTCSSSSSSSEDYMMMYQLPQRRHYGGVRVSYVPNDALAYDRKRKPSELGGDKDKNCIIS 8BFAF1F75F352485 o45030_drome Vang-like protein Vang stbm CG8075 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MENESVKSEHSGRSRRSRNHNNNGGGGGGGGGGVSGGGGSVNNGYHRERDRSRHSHRSTHSSKSAKGFQRGDMAPYQTSVNMTGDGSHDGQEVIEVQILPQDENWGENTTAVTGNTSEQSISMEDINNMWHRESDKGFSFACRRYVESSFYFLLGCGAFFSPVAMVVMPYVGFFPSAFDHPEITQTVRTQLLACSEQCKGQLVSLAARLLLLAIGLWAVFMRRTSATMPRIFLYRALVLLLVTICTFAYWLFYIVQVTNGAKIVVETGGDAVDYKSLVGYATNFVDTLLFIHYVAVVLLELRHQQPCYYIKIIRSPDGVSRSYMLGQLSIQRAAVWVLQHYYVDFPIFNPYLERIPISVSKSQRNKISNSFKYYEVDGVSNSQQQSQSRAVLAANARRRDSSHNERFYEEHEYERRVKKRRARLITAAEEAFTHIKRIHNEPAPALPLDPQEAASAVFPSMARALQKYLRVTRQQPRHTFESILKHLAHCLKHDLSPRAFLEPYLTESPVMQSEKERRWVQSWSLICDEIVSRPIGNECTFQLIQNDVSLMVTVHKLPHFNLAEEVVDPKSNKFVLKLNSETSV DIP protein O45030 original sequence version: 45 71D94D545C3F1DFA dsh_drome Segment polarity protein dishevelled dsh CG18361 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDADRGGGQETKVIYHIDDETTPYLVKIPIPSAQVTLRDFKLVLNKQNNNYKYFFKSMDADFGVVKEEIADDSTILPCFNGRVVSWLVSADGTNQSDNCSELPTSECELGMGLTNRKLQQQQQQHQQQQQQQQQQHQQQQQQQQQQVQPVQLAQQQQQQVLHHQKMMGNPLLQPPPLTYQSASVLSSDLDSTSLFGTESELTLDRDMTDYSSVQRLQVRKKPQRRKKRAPSMSRTSSYSSITDSTMSLNIITVSINMEAVNFLGISIVGQSNRGGDGGIYVGSIMKGGAVALDGRIEPGDMILQVNDVNFENMTNDEAVRVLREVVQKPGPIKLVVAKCWDPNPKGYFTIPRTEPVRPIDPGAWVAHTQALTSHDSIIADIAEPIKERLDQNNLEEIVKAMTKPDSGLEIRDRMWLKITIPNAFIGADAVNWVLENVEDVQDRREARRIVSAMLRSNYIKHTVNKLTFSEQCYYVVNEERNPNLLGRGHLHPHQLPHGHGGHALSHADTESITSDIGPLPNPPIYMPYSATYNPSHGYQPIQYGIAERHISSGSSSSDVLTSKDISASQSDITSVIHQANQLTIAAHGSNKSSGSSNRGGGGGGGGGGNNTNDQDVSVFNYVL 0BA24ED350666CF5 dsh-dgo-1 1 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. S1A dgo-dsh-1 3 4 unspecified role unspecified role bait bait n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 3A dsh-dgo-2 2 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig. 3B, 3C dsh-pk-1 2 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 3B, 3D, 3E dgo competes with pk for binding to dsh. dsh-vang-1 2 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 3B revisit when authors give coordinates of strabismus c-terminal fragment dsh-dgo-3 2 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig. 3D dsh-dgo-4 2 4 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 7 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig. S1D pk competes with dgo for binding to dsh.