UniProt UniProt http://www.uniprot.org Molecular organization of the Ndc80 complex, an essential kinetochore component. Molecular organization of the Ndc80 complex, an essential kinetochore component. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 imex curation Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 Only protein-protein interactions imex curation Molecular organization of the Ndc80 complex, an essential kinetochore component. Molecular organization of the Ndc80 complex, an essential kinetochore component. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 imex curation Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper chromatography chromatography technology column chromatography chromatography predetermined predetermined participant predetermined Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 Only protein-protein interactions imex curation Molecular organization of the Ndc80 complex, an essential kinetochore component. Molecular organization of the Ndc80 complex, an essential kinetochore component. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 imex curation Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Only protein-protein interactions in vitro In vitro chromatography chromatography technology column chromatography chromatography protein sequence protein sequence identification protein sequence Wei RR., Sorger PK., Harrison SC. schadmin@crystal.harvard.edu Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2005 Only protein-protein interactions imex curation nuf2_yeast Kinetochore protein NUF2 YOL069W NUF2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRNQDVFPILDLQELVICLQSCDFALATQENISRPTSDYMVTLYKQIIENFMGISVESLLNSSNQETGDGHLQEENENIYLDTLNVLVLNKICFKFFENIGVQDFNMTDLYKPEAQRTQRLLSAVVNYARFREERMFDCNSFILQMESLLGQLRSKFDDYNLIQQQLKQYEDVDGDNIPDEQELQKLEEQNKELEIQLKKLTKIQETLSIDYNDYKISKQSIFKDLEALSFQIVELESNRDKLIKISNTDMEELSEGIKELNDLLIQRKKTLDDLTAQQKNLQDTVTTFETIISELYDVLRIISSEVQESNRTETELVGLKQNLINNKLKLMNVLETGIMYKLEILQEQLDLQLKNLEKLSQDTKEESRLNDTKLMDLQIKYENEIKPKIDKTDIFIQEELISGKINKLNDEIKQLQKDFEVEVKEIEIEYSLLSGHINKYMNEMLEYMQ D58796E1B5D1E772 ndc80_yeast Kinetochore protein NDC80 NDC80 80 kDa spindle component protein HEC1 Nuclear division cycle protein 80 TID3 Two-hybrid interaction with DMC1 protein 3 YIL144W protein protein yeast Saccharomyces cerevisiae Baker's yeast MQSSTSTDQHVLHHMDPHRFTSQIPTATSSQLRRRNSTNQGLTDMINKSIARNTISGTGIPTGGINKNKRTRSTVAGGTNGTALALNDKSNSRNSVSRLSINQLGSLQQHLSNRDPRPLRDKNFQSAIQEEIYDYLKKNKFDIETNHPISIKFLKQPTQKGFIIIFKWLYLRLDPGYGFTKSIENEIYQILKNLRYPFLESINKSQISAVGGSNWHKFLGMLHWMVRTNIKLDMCLNKVDRSLINQNTQEITILSQPLKTLDEQDQRQERYELMVEKLLIDYFTESYKSFLKLEDNYEPSMQELKLGFEKFVHIINTDIANLQTQNDNLYEKYQEVMKISQKIKTTREKWKALKSDSNKYENYVNAMKQKSQEWPGKLEKMKSECELKEEEIKALQSNISELHKILRKKGISTEQFELQNQEREKLTRELDKINIQSDKLTSSIKSRKLEAEGIFKSLLDTLRQYDSSIQNLTRSRSQLGHNVNDSSLKINISENLLDRDFHEGISYEQLFPKGSGINESIKKSILKLNDEIQERIKTIEKDNITLEKDIKNLKHDINEKTQINEKLELELSEANSKFELSKQENERLLVAQRIEIEKMEKKINDSNLLMKTKISDAEELVTSTELKLEELKVDLNRKRYKLHQQVIHVIDITSKFKINIQSSLENSENELGNVIEELRNLEFETEHNVTN 7DBC492227A80093 7DBC492227A80093 spc24_yeast Kinetochore protein SPC24 SPC24 YM9718.16C YMR117C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQKDNLLDNPVEFLKEVRESFDIQQDVDAMKRIRHDLDVIKEESEARISKEHSKVSESNKKLNAERINVAKLEGDLEYTNEESNEFGSKDELVKLLKDLDGLERNIVSLRSELDEKMKLYLKDSEIISTPNGSKIKAKVIEPELEEQSAVTPEANENILKLKLYRSLGVILDLENDQVLINRKNDGNIDILPLDNNLSDFYKTKYIWERLGK 8BE4B8619DD4C8FB 8BE4B8619DD4C8FB spc25_yeast Kinetochore protein SPC25 YER018C SPC25 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASIDAFSDLERRMDGFQKDVAQVLARQQNHARQQLQQFQAEMRQLHNQHQHLIDELQRLATQRTALQQQIHAAQQATNTTREQWRSYHERESELSRRQSTLAAQSRELDSLLQQRGKECVQLRARWAAQSGNDAAEVALYERLLQLRVLPGASDVHDVRFVFGDDSRCWIEVAMHGDHVIGNSHPALDPKSRATLEHVLTVQGDLAAFLVVARDMLLASL 1BED6CCFF330A5D8 1BED6CCFF330A5D8 ndc80_nuf2_1 2 6 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 7 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper 5 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper 4 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association Tid3 complex Fig. 1A ndc80_nuf2_2 2 5 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 7 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper 4 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association Tid3 complex. Fig. 1B, 1C ndc80_nuf2_3 2 5 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 4 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association Fig. 1B, 1C spc24_spc25_1 2 6 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 7 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association Fig. 1B, 1C ndc80_nuf2_4 1 7 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 5 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper 4 unspecified role unspecified role neutral component neutral component trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association Rod like complex around 570 angstroms in length with globular domains at each end. Tid3 complex. Fig. 2 ndc80_nuf2_5 3 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 6 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) physical association physical association Tid3 complex. Fig. 3B