IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation. Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation. Development (0950-1991) 2004 Han C., Belenkaya TY., Khodoun M., Tauchi M., Lin X., Lin X. linyby@chmcc.org 01-DEC-2006: Contacted by JYOTI. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Han C., Belenkaya TY., Khodoun M., Tauchi M., Lin X., Lin X. linyby@chmcc.org Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Development (0950-1991) 2004 Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation. Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation. Development (0950-1991) 2004 Han C., Belenkaya TY., Khodoun M., Tauchi M., Lin X., Lin X. linyby@chmcc.org 01-DEC-2006: Contacted by JYOTI. drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Han C., Belenkaya TY., Khodoun M., Tauchi M., Lin X., Lin X. linyby@chmcc.org Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Development (0950-1991) 2004 ext1_drome Exostosin-1 ttv CG10117 DEXT1 Protein tout-velu protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQAKKRYILVFVSCAFLAYAYFGGYRLKVSPLRPRRAQHESAKDGGVQPHEQLPSFLGAHDMQELQLLQSNQSKSLDSSKHLVTRKPDCRMETCFDFTRCYDRFLVYIYPPEPLNSLGAAPPTSANYQKILTAIQESRYYTSDPTAACLFVLGIDTLDRDSLSEDYVRNVPSRLARLPYWNNGRNHIIFNLYSGTWPDYAENSLGFDAGEAILAKASMGVLQLRHGFDVSIPLFHKQFPLRAGATGTVQSNNFPANKKYLLAFKGKRYVHGIGSETRNSLFHLHNGRDMVLVTTCRHGKSWRELQDNRCDEDNREYDRYDYETLLQNSTFCLVPRGRRLGSFRFLEALQAGCIPVLLSNAWVLPFESKIDWKQAAIWADERLLLQVPDIVRSIPAERIFALRQQTQVLWERYFGSIEKIVFTTFEIIRERLPDYPVRSSLVWNSSPGALLTLPTFADSSRYMPFLLNSMGAEPRHNYTAVIYVQIGAALGPNAALYKLVRTITKSQFVERILVLWAADRPLPLKKRWPPTSHIPLHVISLGGSTRSQGAGPTSQTTEGRPSISQRFLPYDEIQTDAVLSLDEDAILNTDELDFAYTVWRDFPERIVGYPARAHFWDDSKNAWGYTSKWTNYYSIVLTGAAFYHRYYNYLYTNWLSLLLLKTVQQSSNCEDILMNLLVSHVTRKPPIKVTQRKGYKDRETGRSPWNDPDHFIQRQSCLNTFAAVFGYMPLIRSNLRMDPMLYRDPVSNLRKKYRQIELVGS F6369F0BA206DEA9 o02393_drome Cnx99A CG11958 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAWKMGGNRAATLALLFASSLLLLSSANAADLDTESDDFEDGYVEDVQEEPAVIGGDEKLAYESPVIDAKKFHFADHFDDVEESRKRWVLSQAKKDDIAEEISKYDGIWNWESPQRIVWANDLGLVLKSKAKHAAIAAPLRKPFEFKSDKPLVVQYEVTLQEGQECGGSYLKLLSAGKDTEQLKAFNDKTPYTIMFGPDKCGNDVKMHFIFRHVNPINGTITEKHCNKPKNRLEEPFKDKLPHLYQLVVRPDNSFEIRVDHKIINEGSLLTDFKPPVNPPAEIDDPNDHKPESWDEREKIPDPTAHKPEDWDEDAPPQLPDTDAVMPNGWLEDEPDMIFDPTATKPEDWDAEIDGEWEAPLVDNPVCEKAPGCGKWKAPLIPNPNYKGKWRAPMIENPNYQGKWAPRKIPNPDFFEDLKPFQMTPISAVGLELWSMSSDILFDNLIITDDVEVARDFAANSFDIKRRYIDRESDSFVNKVVELAKANPSIWGIGLVAIVALVALTIYCRFGTAKSQDSAAKKAAAEAKKSDDPQPDDEPEAEEESDERAAGDTSKESTPLSASPKKNQKSDLDDNEEESKAAESREPAQTEESNTKTRKRQARKE 8ED9359840E121FF ext2_drome Exostosin-2 sotv Ext2 Protein sister of tout-velu CG33038 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTKIKNLLSFVTQSRAISHTNPREHILNCLTYGLLVIVALCAGFLLWDLSSSPRDGFFHGKRDSHTLILDLEHIQELAVNPEAEQRARNVNCTFWDCLNIYKCEHDRLKVYIYPLQEFVDEQSDKTATTLSSEYFQILEAVLKSRYYTSNPNEACLFLPSLDLLNQNVFDKHLAGAALASLDFWDRGANHIIFNMLPGGAPSYNTVLDVNTDNAIIFGGGFDSWSYRPGFDVAIPVWSPRLVRQHAHATAQRKFLLVVAQLNILPRFVRTLRELSLAHSEQLLLLGACENLDLTMRCPLSQHHKSLEYPRLLSRGKFCLLGRSLRMGQPDLVEIMSQHCIPVIAVDNYVLPFEDVIDWSLASVRIRENELHSVMQKLKAISSVKIVEMQKQVQWLFSKYFKDLKTVTLTALEVLESRIFPLRARSSRQWNTIDTNARSTFNPLFLPSLAPKSQGFTAVILTYDRVESLFLLIQKLAVVPSLQSILVIWNNQKKSPPHLSTFPSISKPLKIRQTKENKLSNRFYPYPEIETEAILTIDDDIIMLTTDELDFGYEVWREFPDHIVGFPSRIHVWENVTMRWHYESEWTNQISMVLTGAAFHHKYWSHMYTHAMPGDIKDWVDEHMNCEDIAMNFLVANITNNPPIKVTPRKKFKCPECTNTEMLSADLNHMRERSACIDRFSKIYGRMPLRTVEFRADPVLFRDNFPDKLKRYNDIGSL A3894493C16EF7DC ttv-ext 2 5 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 7A Upon immunoprecipitation of Myc-tagged Ttv from the cellular lysate of transfected cells, the Sotv protein could be detected by western blotting in the immunoprecipitate Interaction between Ttv and Sotv was not observed when cellular lysates from individually transfected cells were mixed and immunoprecipitated, this indicated that in vivo presence of both proteins was necessary from the interaction. ttv-ext-1 2 3 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 7A Upon immunoprecipitation of Myc-tagged Ttv from the cellular lysate of transfected cells, the Sotv protein could be detected by western blotting in the immunoprecipitate Interaction between Ttv and Sotv was not observed when cellular lysates from individually transfected cells were mixed and immunoprecipitated, this indicated that in vivo presence of both proteins was necessary fro the interaction. ttv-ext2 1 3 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 7F we found that Ttv and Sotv protein staining were virtually identical, and that they were concentrated in certain compartment(s). ttv-cnx99a 1 3 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component colocalization colocalization 7E Myc-tagged Ttv proteins were present in both endoplasmic reticulum (ER) and Golgi (Fig. 7D-E''). ER marker Calnexin was used.