IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks. Schizosaccharomyces pombe Rdh54 (TID1) acts with Rhp54 (RAD54) to repair meiotic double-strand breaks. Mol. Biol. Cell (1059-1524) 2003 imex curation Catlett MG., Forsburg SL. Strains were initially grown on plates lacking only leucine and tryptophan and then spotted to plates lacking leucine and tryptophan as well as either histidine (his-) or adenine (ade-), or assayed for beta-galactosidase activity (b-gal). forsburg@salk.edu 01-DEC-2006: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Catlett MG., Forsburg SL. Strains were initially grown on plates lacking only leucine and tryptophan and then spotted to plates lacking leucine and tryptophan as well as either histidine (his-) or adenine (ade-), or assayed for beta-galactosidase activity (b-gal). forsburg@salk.edu Accepted 2006-NOV-20 by JYOTI. 01-DEC-2006: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2003 Only protein-protein interactions imex curation rdh54_schpo Meiotic recombination protein rdh54 rdh54 SPAC22F3.03c ATP-dependent helicase rdh54 RAD54 protein homolog 2 Meiotically up-regulated gene 34 protein mug34 tid1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKRRATFQCPLIESTIKHQSTNYTKTETATTSHEVSENANEHKGKSNIDINKIAYYNVVWRKITMKKHKTWEGDGFLVREDSNLTLYNSDFTRLGSCHSRVPQIKEGELLRISGKEISIEKEISAESYEAGTHIDDSLRDVHMPEMKRKFKPPLRPNTMYQTISALPKPRHDPTVLGALVMSRPKTWDPRTHVDVVIDPFLSKHLYSHQREGVSFLYDCLLGMEGKCGYSAILADEMGLGKTLQTITVVWTLLKQSYYANRSSTINNAMVVAPVTLLKNWENEFYNWLGHERIHVYIARCAEDFQEFTSNKTYSIIITGYETVCTYLRNYGCGIDIDLLICDEAHRLKSMSSQTWITLNKLKTRKRLLLTGTPLQNDLSEYFSMVNFIIPGSLGTPNSFKAQYERPILRSRSMNASSRDISLGAARLQRLFEFTSNFTLRRKANILAKHLPPRTDIVLFIKPTHQQENVYGHVLDGFKSSVDQKGYYLKILTRLSKICNSTILLRNEKENFLSTELQDKHVFEQENMLLSSSKLQILAALLKSFQRGCQKAVIVSQYKETLELIELFLSILHVRFCKLLGSTPFSERDLIVHNFNTSSFKEFSVLLLSSKAGGCGLNLTGSTRLIIYEPSWNPAQDLQALSRIYRSGQKRPVCIYTFLSSGMLDERIFIRQNTKQGLSSSFIDSDASQKKNFFTGEDIKTLFSYSKTETCLTYELAFDSDEIDSLTKKKDPLTKIDHTIDSPNTKEKDKEISSWKKASEYMDTNLGKKCPENAFQGWTWQFPNDLSIMNGVEDYIPLEPLPINCLMHKKFE C5C88292A8E93604 rad54_schpo DNA repair protein rhp54 rhp54 rad54 SPAC15A10.03c RAD54 homolog 1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIQQPTTAKPRISTSSKLNTVLSKNKENVPGKLFKKFKCPSLVISEKRKELPLRKKPRVNYSEYGSVDGKYDSAYVSENVSGLATIKEANRLILNHERRDPSTVIKKQFSVPKPIKGHEDISKLCAHRPPPTLGMKRKVDFIPRPLYDPADEFAIVLYDPTTDADEIIPDIKEVLAEKRKKDELLKNRKGKKEISDSEPESDHDSCVSTDTVASCSTEQSLITSNTSKHRRPNKSLKDLLGIQKEKPPPPPVAVVIDPKLARILRPHQIEGVKFLYKCVTGRIDRCANGCIMADEMGLGKTLQCIALLWTLLKQSPQAGKPTIEKAIITCPSSLVKNWANELVKWLGKDAITPFILDGKSSKQELIMALQQWASVHGRQVTRPVLIASYETLRSYVEHLNNAEIGMLLCDEGHRLKNSDSLTFTALDKLNVQRRVILSGTPIQNDLSEYFSLLNFANPGLLGSRQEFRKNYEIPILKGRDADGTEKDKENGDAKLAELAKIVNRFIIRRTNDILSKYLPVKYEHVVFCNLSEFQLSLYKHFITSPEINKILRGTGSQPLKAIGLLKKICNHPDLLNLTEDLEGCEALFPPGFIPRELRGRDRNIDSSLSGKMLVLERMLYQIKQETDDKIVLISNYTSTLDLFEQLCRARGYKALRLDGTMNVNKRQRLVDTFNDPEKDAFVFLLSSKAGGCGINLIGANRLILFDPDWNPAADQQALARVWRDGQKKDCFVYRFIATGTIEEKIFQRQSHKQSLSSCVVDEAQDVERHFSLDNLRQLFQLNDHTVCETHETYKCKRCRDGKQFIRAPAMLYGDTSTWNHFTNPTLDRIEDHLLKREAGKQQVSTVFQYKSH 9DB3A247A57B0880 rti1_schpo DNA repair and recombination protein rti1 rti1 SPBC119.14 Rad twenty-two isogene 1 Rad22 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGSLPDQSSCEEFTDSQQDKMTKLLAMQLGPEYISRRSGPGGGSVTYLEAWKAIELANEIFGFNGWSSSIQDIHVDYVEETKEKKFNVGISVIVRVTLKDGSFHEDVGYGSIENCRVKALAYEKCKKEGTTDALKRALRNFGSSMGNCLYDKRYIQKILKMAPAQAEFNYDNLLRANKRPYARFAQKVSTPIESHANKSVKLEHKNSIEKKISNVDKPISDLIENDIHESLPALQNPPIQSHSETDLYADEELDSILMHHERPPIPESPRVEEFEELLNQFEGDEKVSVDKIDAHDKMTEAQVVKIPPVQFMNARVAAAENPHIKHEGMAFQLHKKSNSILKSSNIDHNRSMPIRRPSLTSNNSANTFSTK 62280F122A2991B0 rad51_schpo DNA repair protein rhp51 rhp51 rad51 SPAC644.14c RAD51 homolog protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MADTEVEMQVSAADTNNNENGQAQSNYEYDVNVQDEEDEAAAGPMPLQMLEGNGITASDIKKIHEAGYYTVESIAYTPKRQLLLIKGISEAKADKLLGEASKLVPMGFTTATEYHIRRSELITITTGSKQLDTLLQGGVETGSITELFGEFRTGKSQICHTLAVTCQLPIDMGGGEGKCLYIDTEGTFRPVRLLAVADRYGLNGEEVLDNVAYARAYNADHQLELLQQAANMMSESRFSLLVVDSCTALYRTDFSGRGELSARQMHLARFMRTLQRLADEFGIAVVITNQVVAQVDGISFNPDPKKPIGGNILAHSSTTRLSLRKGRGEQRICKIYDSPCLPESEAIFAINSDGVGDPKEIIAPV 9F26EB9FA4F3C2BA 9F26EB9FA4F3C2BA dmc1_schpo Meiotic recombination protein dmc1 dmc1 dmp1 SPAC8E11.03c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEEFAEGNDDEQMIFSDIEDLTAHGIGMTDIIKLKQAGVCTVQGVHMSTKRFLLKIKGFSEAKVDKLKEAASKMCPANFSTAMEISQNRKKVWSISTGSEALNGILGGGIQSMSITEVFGEFRCGKTQMSHTLCVTAQLPRDMGGAEGKVAFIDTEGTFRPDRIKAIAERFGVDADQAMENIIVSRAYNSEQQMEYITKLGTIFAEDGQYRLLIVDSIMALFRVDYSGRGELSERQQKLNIMLARLNHISEEFNVAVFVTNQVQADPGAAMMFASNDRKPVGGHVMAHASATRLLLRKGRGEERVAKLNDSPDMPEAECSYVITPGGIADVS 86B73EAA491F7B02 86B73EAA491F7B02 rad51_human DNA repair protein RAD51 homolog 1 RAD51 RAD51A RECA RAD51 homolog A protein protein human Homo sapiens Human MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD DIP protein Q06609 original sequence version: 154 DIP protein Q06609 original sequence version: 145 DIP protein Q06609 original sequence version: 122 DIP protein Q06609 original sequence version: 121 DIP protein Q06609 original sequence version: 125 DIP protein Q06609 original sequence version: 123 DIP protein Q06609 original sequence version: 144 DIP protein Q06609 original sequence version: 167 DIP protein Q06609 original sequence version: 169 26578E6206DEDEDA rad22_schpo DNA repair and recombination protein rad22 rad22 SPAC30D11.10 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSFEQKQHVASEDQGHFNTAYSHEEFNFLQSSLTRKLGPEYVSRRSGPGGFSVSYIESWKAIELANEIFGFNGWSSSIRSINVDFMDENKENGRISLGLSVIVRVTIKDGAYHEDIGYGSIDNCRGKASAFEKCKKEGTTDALKRALRNFGNSLGNCMYDKYYLREVGKMKPPTYHFDSGDLFRKTDPAARESFIKKQKTLNSTRTVNNQPLVNKGEQLAPRRAAELNDEQTREIEMYADEELDNIFVEDDIIAHLAVAEDTAHPAANNHHSEKAGTQINNKDKGSHNSAKPVQRSHTYPVAVPQNTSDSVGNAVTDTSPKTLFDPLKPNTGTPSPKFISARAAAAAEGVVSAPFTNNFNPRLDSPSIRKTSIIDHSKSLPVQRASVLPIIKQSSQTSPVSNNSMIRDSESIINERKENIGLIGVKRSLHDSTTSHNKSDLMRTNSDPQSAMRSRENYDATVDKKAKKG E6B8B538D28B899D rdh54-dmc1 1 6 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait physical association physical association Figure 3 On plates lacking histidine, and thus requiring activation of the least stringent reporter for cell growth, we saw that Rdh54 interacted with both Dmc1 and Rhp51. On selection for ade or for -galactasidase activity, however, Rdh54 only interacted with Dmc1 (Figure 3). These data suggest that Rdh54 can interact with both Dmc1 and Rhp51 but that the interaction is stronger between Rdh54 and Dmc1. rdh54-rad51 1 2 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Figure 3 On plates lacking histidine, and thus requiring activation of the least stringent reporter for cell growth, we saw that Rdh54 interacted with both Dmc1 and Rhp51. On selection for ade or for -galactasidase activity, however, Rdh54 only interacted with Dmc1 (Figure 3). These data suggest that Rdh54 can interact with both Dmc1 and Rhp51 but that the interaction is stronger between Rdh54 and Dmc1. This interaction was observed on a his- plate rad54-rad51 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Figure 3 Rhp54 interacted only with Rhp51 and not Dmc1 (Figure 3) dmc1-dmc1-4 1 6 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Figure 3 rhp51-rhp51-4 1 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Figure 3 Rhp51 also gave a positive signal with itself, with Rti1, and with Rad22 (Figure 3). rhp51-rti1 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Figure 3 Rhp51 also gave a positive signal with itself, with Rti1, and with Rad22 (Figure 3). This interaction was observed on an ade- plate. rhp51-rad22 1 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Figure 3 Rhp51 also gave a positive signal with itself, with Rti1, and with Rad22 (Figure 3).