IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol. Biol. Cell (1059-1524) 2003 imex curation Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca fluca@vet.upenn.edu or charlie.boone@utoronto.ca Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Yeast cDNA library derived from pACT was used. Mol. Biol. Cell (1059-1524) 2003 Only protein-protein interactions imex curation RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol. Biol. Cell (1059-1524) 2003 imex curation Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca fluca@vet.upenn.edu or charlie.boone@utoronto.ca Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2003 Only protein-protein interactions imex curation RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. RAM: a conserved signaling network that regulates Ace2p transcriptional activity and polarized morphogenesis. Mol. Biol. Cell (1059-1524) 2003 imex curation Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca 23-MAY-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nelson B., Kurischko C., Horecka J., Mody M., Nair P., Pratt L., Zougman A., McBroom LD., Hughes TR., Boone C., Luca FC. Plasmids encoding positive interactors were sequenced. fluca@vet.upenn.edu,charlie.boone@utoronto.ca fluca@vet.upenn.edu or charlie.boone@utoronto.ca Accepted 2007-JUN-8 by DAVET. 23-MAY-2007: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2003 Only protein-protein interactions imex curation tao3_yeast Cell morphogenesis protein PAG1 YIL129C TAO3 PAG1 Protein TAO3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASRFTFPPQRDQGIGFTFPPTNKAEGSSNNNQISIDIDPSGQDVLEEINEAPLNTFPLHQSVTDAPIIDIPSPTDMSEGTSLNNQLLLRQQQQQGTGEGQALPPTFVEEQSDQNKISMLLPEQKQQRMQESAPPDITAKSVAEDYVTTLRQQMATDWKSPSEYALHILFTKFIRYAENKLNMCLQQLDMAEPPIVEILGEGVDPSFDEIIKSLGHIAKKKPKPVIDAMMFWRKTKSEAANSASEEMEKLLKEYEFEKAHPSQAHFLMNRRLSRSSSNTTSKYKHNNNTNNLPGMKRHVSSSFNNKVPLIKASSSNNSATSSPSIANSQLKSLENTIEVAKEEAFLADRKSLISIYILCRVLNEIVKQASSNEEEDLSDKLEEIVFTQLKTTDPLSISTSLIKSSNWNSFAELLGSMSEKKFLSVSDRFIADLEKIPAYIPPELEPSTHLLILGMRYLKLRNYPLEKFEESADFMKSLSKFFAKTENFPVCLAYAEVTNQLLLPLAGSLTAEVNHPTWVEAMSTLLNTAKRLQADSKYWVSGFKLTVSILCASPPDLFSKQWLSLLEANASKVKSKSLNERIIFAVGLSRLVWVYLYRCPETLNNTTRTLTKLLQLYLNTRKKENWITGDFGLLNPLTDALISIGFLHPNFLMEQALIPLIRQSFNGSNLENINYEKLILTINTYKGLLVTKERPRFPEDDNRLYELNLNNITVNQVQEASSINHTEISDYFYKLFLLLDSSIGSEVWSPENQHQKQSSNAFSPFGFSFSNDNDSSKNKSLYVILFGTIIEAIPCCLSISRTIPYKSTIEILSRNAVHSEVIISSSSQNALRALASKKNPYTLITWFAKYSFDFDEKTQSSYNMSYLSSKEYNRLLILYVELLECWLEEFQSSNKEENKKETGLDGIRLLPIDAEQEESNETEKLEWKNTVTVIEEVEGNGLFFLCSHDAKIRRLGIQILRIIFKFDEAMMEKTEKLSNGHSRSSSHFAADRGTRLIDLLNECNTTTLINPHKATLSAVEKTRFSRLNSKYKRGLLIKLAESEYGVDAALWQRAFPKLLALVFKTCPMAMALCRSIVCIRLVQVHEIILRVANDVDFKLKNVLPETIVNQWKLYLIAACTSLTSTFDQKLHIPSNIPQHGRKKSQQIFTVQHQKIKSAKSIFKMVLPLLNAKYIMIRDAIITGLSSMNINIFKAYVEAIDVFLVAWKEGSSNNQIRVEMFHILTILSPYLKSDMIFNDEWILRKLSEFLQKTKQFLEKDSVQISYEYQSLRSYFAGLILSYYMAVREHPLIDELFPFQARASCFNFLKEWCGYGEYEPISEERYAIMIKNTESGRDRTAITTGIEFQKNRLQMIVLETMVVLCSDPITQTLDDDLELPIVISFDTEDLLAWIEALFDSDNTTVKNLGVRALENLLDKNRENFKLFRDVAFQCVSHHSHPSVAVLYYTTLCKSVLKLDNLVLDEDELVSLGLYGLVADKEDTRTFAVDLLSAVETKLHNSSYTKVFKERLANSSKTVYKSTAKEISSIFAELLSQDLCLRIFSSLVRILDLFPFEIKRDLLVLMVPWVNKFTLKSLEELDTFMVLNNLFYITIDLNDSLPNEVEQLWISLGKGNSFQNIHVSLEYIINSSMNHCNPLFVQYARDIVLYLANIPGGIGLLDTLLNNLEPKYMVPLAKHTFNEPMNNNKYSFLGNIWERLNYNGKRIIFSKAQLSIIFLVNLLTNLSESVKAKIPLLLHMSICLLDHYVPLIHESACKIASTLIFGLAPSHEKSEETVKLLRNKHALWSYDNLMKKGARSPKTMDLLIRNIISIFSDLDEFQVTWQRIALKWATTCSVRHIACRSFQIFRSLLTFLDQEMLRDMLHRLSNTISDGNVDIQGFAMQILMTLNAIMAELDPTNLISFPQLFWSITACLSSIHEQEFIEVLSCLSKFISKIDLDSPDTVQCLVAIFPSNWEGRFDGLQQIVMTGLRSANSLEITWKFLDKLNLLKDSRIIANTESRLLFALIANLPRFLNAMDRKDFTGIQVAADSLIELANAYKQPSLSRLIDSLAKNKFRSKKDFMSQVVSFISRNYFPSYSAQTLVFLLGLLFNKIGWIRVQTLEILKYVFPLIDLRRPEFIGVGADLISPLLRLLFTEYEAKALEVLDCVPNVSGSKMDKDVLRITMGNKDVKDGDNATTTLFGLPEDSGWSVPMPTMTAATTRHNVHAVFMTCGTGKSDEVSAHGSDDMDAVIEFHADGDYELGRMDTIVEFHADGDYDLGRMDTNDSISVAEEKDASLSHMWAELDNLDSFFTKDTNVPNISSKMGMGIPHGRSDSIETTRTDQTFSFESAPQLYDKKVSVILNRSLSRTPSNVSFKTHLADSFAVKINRNGKPRI 08958191BE969C1B 08958191BE969C1B sog2_yeast Leucine-rich repeat-containing protein SOG2 SOG2 YOR353C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVATSSKRTLDPKEEHLPADKTSTNSSNTIISELATQEKSSSSGTTLKLIALNIKSISDEDVGYIQNVERLSLRKNHLTSLPASFKRLSRLQYLDLHNNNFKEIPYILTQCPQLEILDLSSNEIEALPDEISSFWQDNIRVLSLKDNNVTSIRNLKSITKLNKLSILDLEDNKIPKEELDQVQSYTPFHTGIPKEEYWAIAISRYLKDHPNLPTPEPKISRAAKRMGFINTNLSNGAMNENNIISLAPSANTTISASTAMVSSNQTSATSFSGTVNAESEQSGAVNGTELYNHTKYNDYFKRLSILPEESMSNGHQKISHAELVVSCRKLLFSFTECQQAIRKIASFCKEKAVAVNVVSLLYSVRSHTDNLVEVLQQTENEDESHDQALIKLCLTIITNFKQIITLLRKNFEIFFKEDDLCFIRMFYMTLMCAYMEMYNAWSFIKEDDQVSGSASKAPKKHSFSRHETSSSSITSGGGPAASTTSTHCSGNIKLLPKTRSTRTPSASALLSNSNILTGDTTAVPLLSPNLNGAHTHGPILGHQNAISNGSSQTNMNEVKTTSDTIPRQQLLQHNKSISDSKKESQAHEPKQHPVMTSSIINASNSNNVSNVNITPPPMNGGGAANSSANVVETNIDIQLYQTLSTVVKMVSVVYNQLTSEISKIAIASTMGKQILTDSLAPKIRDLTETCRQAMDLSKQLNERLNVLIPNDSNSEKYLTSLEKLKTWEIMNSFLKVIISILANTKIVMSDVPNLNELRPNLANLAKITKDVTVILDLSSYKAVSVSANSPE B0EA559AA4F66199 B0EA559AA4F66199 mob2_yeast CBK1 kinase activator protein MOB2 YFL035C YFL035C-A YFL034C-B MOB2 MPS1 binder 2 Maintenance of ploidy protein MOB2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFFNFKAFGRNSKKNKNQPLNVAQPPAMNTIYSSPHSSNSRLSLRNKHHSPKRHSQTSFPAQKSTPQSQQLTSTTPQSQQQEASERSESQQIMFLSEPFVRTALVKGSFKTIVQLPKYVDLGEWIALNVFEFFTNLNQFYGVVAEYVTPDAYPTMNAGPHTDYLWLDANNRQVSLPASQYIDLALTWINNKVNDKNLFPTKNGLPFPQQFSRDVQRIMVQMFRIFAHIYHHHFDKIVHLSLEAHWNSFFSHFISFAKEFKIIDRKEMAPLLPLIESFEKQGKIIYN CFBA15FF83BFAA33 CFBA15FF83BFAA33 cbk1_yeast Serine/threonine-protein kinase CBK1 CBK1 YNL161W N1727 Cell wall biosynthesis kinase protein protein yeast Saccharomyces cerevisiae Baker's yeast MYNSSTNHHEGAPTSGHGYYMSQQQDQQHQQQQQYANEMNPYQQIPRPPAAGFSSNYMKEQGSHQSLQEHLQRETGNLGSGFTDVPALNYPATPPPHNNYAASNQMINTPPPSMGGLYRHNNNSQSMVQNGNGSGNAQLPQLSPGQYSIESEYNQNLNGSSSSSPFHQPQTLRSNGSYSSGLRSVKSFQRLQQEQENVQVQQQLSQAQQQNSRQQQQQLQYQQQQQQQQQQQHMQIQQQQQQQQQQQQSQSPVQSGFNNGTISNYMYFERRPDLLTKGTQDKAAAVKLKIENFYQSSVKYAIERNERRVELETELTSHNWSEERKSRQLSSLGKKESQFLRLRRTRLSLEDFHTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAERDVLAGSDSPWVVSLYYSFQDAQYLYLIMEFLPGGDLMTMLIRWQLFTEDVTRFYMAECILAIETIHKLGFIHRDIKPDNILIDIRGHIKLSDFGLSTGFHKTHDSNYYKKLLQQDEATNGISKPGTYNANTTDTANKRQTMVVDSISLTMSNRQQIQTWRKSRRLMAYSTVGTPDYIAPEIFLYQGYGQECDWWSLGAIMYECLIGWPPFCSETPQETYRKIMNFEQTLQFPDDIHISYEAEDLIRRLLTHADQRLGRHGGADEIKSHPFFRGVDWNTIRQVEAPYIPKLSSITDTRFFPTDELENVPDSPAMAQAAKQREQMTKQGGSAPVKEDLPFIGYTYSRFDYLTRKNAL 87EBCD2C3C96EE11 87EBCD2C3C96EE11 kic1_yeast Serine/threonine-protein kinase KIC1 Kinase that interacts with CDC31 N-rich kinase 1 YHR102W KIC1 NRK1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTKPQNSKQGLAEGEMDVSSLFKRTEVIGRGKFGVVYKGYNVKTGRVYAIKVLNLDSDSDEVEDVQREIQFLASLKQISNITRYYGSYLKDTSLWIIMEHCAGGSLRSLLRPGKIDEKYIGVIMRELLVALKCIHKDNVIHRDIKAANVLITNEGNVKLCDFGVAAQVNQTSLRRQTMAGTPYWMAPEVIMEGVYYDTKVDIWSLGITTYEIATGNPPYCDVEALRAMQLIIKSKPPRLEDRSYSTSLKEFIALCLDEDPKERLSADDLLKSKFIRAHKATPTSILKELISRYLLFRDKNKNKYKIEGSIPENEPSKPSEAPKPSQNGGGDEAQKSIASNDNEIKRVNEGDVEMKWDFDSLSSSDYIIENNINLDALAEDNNEWATAQHDLFNYAYPDEDSYYFDPTSHNTRPFVYQGTTIGKGYPGTIAQNSTLNAPVTNNYTNSKYPSKMVAGTTNTSGTHTAGPMTSSKRLESKAPKQLLELFEDNEIITAENDVNTEAPKISKSISSLNAGNSSRDDFIPSISNEVNGNINNNKMRPHLPPLSSGNNYYSQSTPALPLLQTKFNKTSKGPPTSGLTTAPTSIEIEIPEELPNSALPTPASADPVLIPSTKARSSTVTAGTPSSSSSIQYKSPSNVPRRLTVSNNRPEHCPSTITNQKLGSAVASNSGISSTPNNSNNYNNNTDSENSRGSSGSNTANSTQMGITNPGNVTKLSTHKASSPSRPLFGVGTSPNRKPAGSPTQNIGHNSTHTNLAPPPTMKPMANSKDNKDILLQPLNSIPSSSTLNTISGNSSNNLTSSNYFSNEKEGSRVNGDFKRNNPNLKLQMPLPTPVVRNKLLDPNTATSQNNNGMPGSAGISTNENINQFGFNTSSASNIPVSMTPISEKHIDFGGKIKRSQSISNRKNSSASEHPLNILGSSVSGNVSGIGNNNVGSNNNSGPNNSVPLSANTGNTTIKANSTTIATSSSAAASTTAPISQQTIPSGTQFNHILSSAATAANSVNSLGFGMCPPPQSLQMEMFLDLESFLPGKQRRVDRKPQVLKELENLLQMFEEGLPCIEHALKEQLISTPIKDNEH 9989EAF315EE0B94 9989EAF315EE0B94 hym1_yeast Protein HYM1 YKL189W HYM1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFKKYKNQDLDMAFWWKKNPKTPSDYARLIIEQLNKFSSPSLTQDNKRKVQEECTKYLIGTKHFIVGDTDPHPTPEAIDELYTAMHRADVFYELLLHFVDLEFEARRECMLIFSICLGYSKDNKFVTVDYLVSQPKTISLMLRTAEVALQQKGCQDIFLTVGNMIIECIKYEQLCRIILKDPQLWKFFEFAKLGNFEISTESLQILSAAFTAHPKLVSKEFFSNEINIIRFIKCINKLMAHGSYVTKRQSTKLLASLIVIRSNNALMNIYINSPENLKLIMTLMTDKSKNLQLEAFNVFKVMVANPRKSKPVFDILVKNRDKLLTYFKTFGLDSQDSTFLDEREFIVQEIDSLPRIISSTTEVSNNNASSSNVASITSPSSVMNNQSSILTHSTSPDSR F48860754C892BA9 F48860754C892BA9 hym1-kic1-1 2 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. cbk1-cbk1 2 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. tao3-kic1-1 2 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. tao3-cbk1-1 2 7 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. hym1-kic1 3 9 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Figure 6B. tao3-cbk1-2 2 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. tao3-kic1 2 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. hym1-sog2 2 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Figure 6A. cbk1-cbk1-1 2 7 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. cbk1-kic1 2 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 6A. cbk1-cbk1-2 2 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association Figure 6A. cbk1-mob2 2 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association Figure 6A. cbk1-sog2 2 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Figure 6A. hym1-sog2-1 1 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association