UniProt UniProt http://www.uniprot.org TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. Curr. Biol. (0960-9822) 2003 imex curation Bellanger JM., Gonczy P. pierre.gonczy@isrec.unil.ch 21-Dec-2010: Contacted by JYOTI. Only protein-protein interactions caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. fluorescence imaging fluorescence microscopy fluorescence imaging polyclonal immunost polyclonal antibody immunostaining polyclonal immunost Bellanger JM., Gonczy P. pierre.gonczy@isrec.unil.ch Accepted 2006-OCT-9 by DAVET. 21-Dec-2010: Contacted by JYOTI. Curr. Biol. (0960-9822) 2003 Only protein-protein interactions imex curation TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. Curr. Biol. (0960-9822) 2003 imex curation Bellanger JM., Gonczy P. pierre.gonczy@isrec.unil.ch 21-Dec-2010: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bellanger JM., Gonczy P. pierre.gonczy@isrec.unil.ch Accepted 2006-OCT-9 by DAVET. 21-Dec-2010: Contacted by JYOTI. Curr. Biol. (0960-9822) 2003 Only protein-protein interactions imex curation TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. TAC-1 and ZYG-9 form a complex that promotes microtubule assembly in C. elegans embryos. Curr. Biol. (0960-9822) 2003 imex curation Bellanger JM., Gonczy P. pierre.gonczy@isrec.unil.ch 21-Dec-2010: Contacted by JYOTI. Only protein-protein interactions caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Bellanger JM., Gonczy P. pierre.gonczy@isrec.unil.ch Accepted 2006-OCT-9 by DAVET. 21-Dec-2010: Contacted by JYOTI. Curr. Biol. (0960-9822) 2003 Only protein-protein interactions imex curation tacc1_caeel Transforming acid coiled-coil-containing protein 1 2P40 tac-1 Y54E2A.3 protein protein caeel Caenorhabditis elegans MSLNTTFTKEDGTEVVIPFNGSQNGHPENEEPEVEEAAEPSSSVETLCGATRGDIIVMKHTTKALTELIERLLHSDEFEVRRCSNGQIISQGRCNGTTPGNGIGGGGASSEELEKALKDRDAARAEADKLHANYATLFASFNTVREAANDIRGEYEDARDKLKLAAAEVDEWQAKFLAVKDNANSELERASVEYDDLLRSHDENTKGLRLRVKRQEIELSSKNDEIKVLTNRVSELSQICDQLLNDVDVSDGMSVISTDA 31D20FF3E5AF562B zyg9_caeel Zygote defective protein 9 zyg-9 F22B5.7 protein protein caeel Caenorhabditis elegans MSNWDYLDEVDILPKLPPNFDELRESKKWQERKEALEALLKVLTDNERLSTKASYAELIGHLQMVLAKDANINCQALAAKCIGKFATGLRAKFSSFAGPLLPVIFEKMKEKKPMLREPLVDCSNEVGRTMQSLETGQEDILAALAKPNPQIKQQTALFVARQLDLVVPAKQPKGFIKAVVPVFGKLTGDADQDVREASLQGLGAVQRIIGDKNVKNLLGDASSDEGKMKKIGEYAEKSTASFAEEQAKNAPPVAPTSSTPSASAASGDPSGGTATAVVSSGAPVAEADPWDFLDAFDVLSKMPDGFDTNIESKKWQERKEALEGLLQLITANPKLDPKANYGALVERLQKVLEKDANINVAALAANCITGIANGLRTKFQPFAVSVTPIIFEKFKEKKPTLRDPLVACIDAVVATTNLEAVGEIVLAALGKPNPSIKTQTDLFLQRCFMKLNSQTMPKKTLKTLIPSLIKHSGDSDSEVREASYAAMGAMMRAIGEKPSLQLLADIASDNLKMSKIKEFHQKALDEAGPAEIAEMVKSIHKADAPPAAAPPKKTAPPKKQPEDEEVVEEEDEPLKPPPGDKKKKVPVKENEENEPPVVAPKAELLLSDNEDKKQRIKEEKQLKLVKWNFQAPTDEHISQLQTLLGNQAKVSLMSQLFHKDFKQHLAALDSLVRLADTSPRSLLSNSDLLLKWCTLRFFETNPAALIKVLELCKVIVELIRDTETPMSQEEVSAFVPYLLLKTGEAKDNMRTSVRDIVNVLSDVVGPLKMTPMLLDALKSKNARQRSECLLVIEYYITNAGISPLKSLSVEKTVAPFVGDKDVNVRNAAINVLVACFKFEGDQMWKAAGRMADKDKSLVEERIKRTGVKPGSGVVTSPPTGGPKILVPQQQGSVVRRPASRSRTREPEPEEVQSDTFTIRQDTMPPKTSSRYALRDDVFSSAMGRLDGTQVITPPQPVNGWSNNTFQMKRTNSSSSISSIDTSDQIQRSINNISSSLADVAQDAMFQVTYVLNQPEQRHLVDRRADLVFRASAAQLDLVIEEFNAGRDVSGTMDACTQMLFILMGGVETEHGLEPLNASPDTVKAIISSVLRCIIQIGNTESGYGMARSLNRLAMRLIYRVELSNLLCGLILAMTESLQMNTGITELVSKLSSKWCDELEKRRAQLRASDIVDSFNAFYVCALTELKMDISDSHILIVDNYLERVILQQGDVVLDAARRLSRPHMHLTSMINKILQMMRERKIDPIMPGTLEARMPQEDEAVVVRSGVQVSIDNILRDTSMAVKHIEQLNILIASSDRSWNEYMEYLKNNPMGELIKELVGECSRKKRIDFNLSHVVKSSMAVFKAMAATGPVQEEGRITPTDINRMDTMIVGTPLSRGDATITRARGNMIRPKRTTLSRDQMANIRHTLDRVKNH DIP protein G5EEM5 original sequence version: 30 9518FFA233ABB3A6 tac-1_zyg-9_1 1 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. S2A tac-1_zyg-9_2 2 4 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes physical association physical association Fig. 5A, 5B tac-1_zyg-9_3 3 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 5C zyg-9_tac-1_1 3 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 5C