UniProt UniProt http://www.uniprot.org The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. (0890-9369) 2003 McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu xenla Xenopus laevis (African clawed frog) African clawed frog fluorescence imaging fluorescence microscopy fluorescence imaging polyclonal immunost polyclonal antibody immunostaining polyclonal immunost McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu Accepted 2006-OCT-9 by JYOTI. Genes Dev. (0890-9369) 2003 The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. (0890-9369) 2003 McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu Accepted 2006-OCT-9 by JYOTI. Genes Dev. (0890-9369) 2003 The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. (0890-9369) 2003 McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu xenla-oocyte Xenopus laevis oocyte Oocyte cosedimentation cosedimentation polyclonal western polyclonal antibody western blot polyclonal western McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu Accepted 2006-SEP-26 by JYOTI. Genes Dev. (0890-9369) 2003 The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. (0890-9369) 2003 McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. pts7h@virginia.edu xenla-oocyte Xenopus laevis oocyte Oocyte molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column polyclonal western polyclonal antibody western blot polyclonal western McCleland ML., Gardner RD., Kallio MJ., Daum JR., Gorbsky GJ., Burke DJ., Stukenberg PT. A superose 6 column was used. pts7h@virginia.edu Accepted 2006-SEP-26 by JYOTI. Genes Dev. (0890-9369) 2003 ndc80_xenla Kinetochore protein NDC80 homolog kntc2 Kinetochore protein Hec1 Kinetochore-associated protein 2 ndc80 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRRSSVTNRQSLLPLRVQDANRMGLTTPQNKDRQGFGKLSMSKPHSGTSEKKTSFFGKRASNGTARTSQYGAFGGTEKIKDPRPLHDKAFIQQCIRQLCEFLNENGYSQALTVKSLQGPSTKDFLKIFAFIYTFICPNYENPESKFEEEIPRIFKELGYPFALSKSSMYTVGAPHTWPQIVAALVWLIDCVKLCCVLRSENPVFEDPPMGEQSENGIDFNQLFLDYTVRCYDQFMEGRDTFEEYDTDVCIRLKEAYHVDESNLEALQQESRRLMEEIERLEKEKENEPDRLASMRKLKASLQADIQKYQNYLTEIESHSTLLDQRVSSISEELEATELESRAIQQENLRLKNILDNQKYSVADIERIKYEETELQQTIAKLTKDLDEDKQQLWHEELKYAKMKESVETQLSEFHKIGRKVRLIPPTAEFANGYDFQIQCNLDSEQSSLMHYRNKINVPLVEILSQSEGHIASSTNKKMGVEDMVEQLNTLIGEKKNEVKVQKDEAQKLEEIYQQKVEESEEHEKRWISEIESLEKHRQLLESGVNKSLDEAMKDLQKEQQELQLVEHRTEEEMRQVENKLVRVVHAVANHVAVIEKHLEEKRLKVEREYEEFMKEDLLLDLRELLEKYKEKARVLDSL 6D725844D425F29E nuf2a_xenla Kinetochore protein Nuf2-A Cell division cycle-associated protein 1-A nuf2-a cdca1-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDKLTFPIFPAADLVNFFRQNILTGTEAKNFNKNDIFPNPKPEMVQKLYMRILQQVFNYGVEQFYMVPMDLDIQYPHLVEGFAPVANILKLMARFLPMCRVYDFHPSDVLNPKGKRTLHSLSGIVNFLHFSATRKEVYFEYCSSYKSALENVRQLQKANQEAEIKIEKLTTVPPEQQAEFKALSSEIHDLQQIISQEYRAKDVAFQEKIAQRKTEFAEKNKRLNEQKLAIATMKEEQERMKSQIVESPEQRKSKTERMKETVHRLKQARQETNDKCDYYRDRVAFACMWQTDVQGYLKKLQGIDTNLEIHRKIREEIRHSEEQVVNLNLELKSLSNEDAQLKRIILVKKEKLAKVDIKNKKKQEDFNQQKQEILEVCSRIQEKRQVVHGRVAQVLQEIQQTIGKKEQLLETTEAGKKKCQEVITDFRAALEKYHDSLQKASERSADRRREKIAELNRRLSRR 4C2E5299D9ED6CB0 kntc2-cdca1-7 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 1B cdca1-kntc2-2 2 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Fig. 1B kntc2-cdca1-8 3 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1C kntc2-cdca1-9 4 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component physical association physical association Supplementary Fig. 2A kntc2-cdca1-10 1 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Supplementary Fig. 2D