IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Multistep assembly of the protein import channel of the mitochondrial outer membrane. Multistep assembly of the protein import channel of the mitochondrial outer membrane. Nat. Struct. Biol. (1072-8368) 2001 Model K., Meisinger C., Prinz T., Wiedemann N., Truscott KN., Pfanner N., Ryan MT. Radiolabelled protein reassembled into complexes in the mitochondria. pfanner@uni-freiburg.de yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip weight by staining molecular weight estimation by staining weight by staining Model K., Meisinger C., Prinz T., Wiedemann N., Truscott KN., Pfanner N., Ryan MT. Radiolabelled protein reassembled into complexes in the mitochondria. Radiolabelled protein reassembled into complexes in the mitochondria. pfanner@uni-freiburg.de Accepted 2007-JAN-23 by JYOTI. Nat. Struct. Biol. (1072-8368) 2001 tom40_yeast Mitochondrial import receptor subunit TOM40 TOM40 ISP42 YMR203W YM8325.04 MOM38 Mitochondrial import site protein ISP42 Translocase of outer membrane 40 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAPTPLAEASQIPTIPALSPLTAKQSKGNFFSSNPISSFVVDTYKQLHSHRQSLELVNPGTVENLNKEVSRDVFLSQYFFTGLRADLNKAFSMNPAFQTSHTFSIGSQALPKYAFSALFANDNLFAQGNIDNDLSVSGRLNYGWDKKNISKVNLQISDGQPTMCQLEQDYQASDFSVNVKTLNPSFSEKGEFTGVAVASFLQSVTPQLALGLETLYSRTDGSAPGDAGVSYLTRYVSKKQDWIFSGQLQANGALIASLWRKVAQNVEAGIETTLQAGMVPITDPLMGTPIGIQPTVEGSTTIGAKYEYRQSVYRGTLDSNGKVACFLERKVLPTLSVLFCGEIDHFKNDTKIGCGLQFETAGNQELLMLQQGLDADGNPLQALPQL 6436FBBA4020464B tom22_yeast Mitochondrial import receptor subunit TOM22 MOM22 TOM22 MAS17 MAS22 YNL131W N1217 N1862 Mitochondrial 22 kDa outer membrane protein Translocase of outer membrane 22 kDa subunit Protein MAS17 Mitochondrial 17 kDa assembly protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLDRIVALKDIVPPGKRQTISNFFGFTSSFVRNAFTKSGNLAWTLTTTALLLGVPLSLSILAEQQLIEMEKTFDLQSDANNILAQGEKDAAATAN 6ACEC5D37D143CD3 6ACEC5D37D143CD3 tom20_yeast Mitochondrial import receptor subunit TOM20 MOM19 YGR082W TOM20 MAS20 Mitochondrial 20 kDa outer membrane protein Translocase of outer membrane 20 kDa subunit Protein MAS20 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQSNPILRGLAITTAIAALSATGYAIYFDYQRRNSPQFRKVLRQRAKEQAKMEEQAKTHAKEVKLQKVTEFLSMELAKDPIPSDPSEREATFTTNVENGERLSMQQGKELEAASKFYKALTVYPQPADLLGIYQRSIPEAIYEYIILMIAILPPANVASFVKGVVGSKAESDAVAEANDIDD 9F76FE6010278410 tom5_yeast Mitochondrial import receptor subunit TOM5 YPR133W-A TOM5 MOM8A Translocase of outer membrane 5 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MFGLPQQEVSEEEKRAHQEQTEKTLKQAAYVAAFLWVSPMIWHLVKKQWK E1419ED5FEC45E14 E1419ED5FEC45E14 tom5-tom40-1 1 2 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait physical association physical association 6A Radiolabeled Tom40 accumulated in the 400K complex of wild type mitochondria was efficiently coprecipitated by anti-Tom5 This interaction was seen in both the 100/250kDa complex and the 400kDa complex formed after 60 minutes. tom20-tom40-2 1 4 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 6A Significant amounts of Tom40 were also coprecipitated by anti-Tom20 and anti-Tom22 (Fig. 6a, lanes 8 and 9). This interaction was seen in the 400kDa complex which formed after 60 minutes. tom22-tom40-1 1 3 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 6A Significant amounts of Tom40 were also coprecipitated by anti-Tom20 and anti-Tom22 (Fig. 6a, lanes 8 and 9). This interaction was seen in the 400kDa complex which formed after 60 minutes.