UniProt UniProt http://www.uniprot.org Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast. Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast. J. Cell Biol. (0021-9525) 2001 imex curation Farkasovsky M., Kuntzel H. kuentzel@em.mpg.de 25-AUG-2004: Contacted by JYOTI. Returned mail. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Farkasovsky M., Kuntzel H. kuentzel@em.mpg.de Accepted 2006-AUG-1 by JYOTI. 25-AUG-2004: Contacted by JYOTI. Returned mail. J. Cell Biol. (0021-9525) 2001 Only protein-protein interactions imex curation bni1_yeast Protein BNI1 PPF3 SHE5 BNI1 YNL271C N0646 Synthetic lethal 39 Sensitive to high expression protein 5 Pointed projection formation protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLKNSGSKHSNSKESHSNSSSGIFQNLKRLANSNATNSNTGSPTYASQQQHSPVGNEVSTSPASSSSFRKLNAPSRSTSTEARPLNKKSTLNTQNLSQYMNGKLSGDVPVSSQHARSHSMQSKYSYSKRNSSQASNKLTRQHTGQSHSASSLLSQGSLTNLSKFTTPDGKIYLEMPSDPYEVEVLFEDIMYKRNIFQSLSEDKQEALMGYSIEKKWLIVKQDLQNELKKMRANTTSSSTASRTSMASDHHPILTANSSLSSPKSVLMTSASSPTSTVYSNSLNHSTTLSSVGTSTSKGKKLVSGSLKKQPSLNNIYRGGAENNTSASTLPGDRTNRPPIHYVQRILADKLTSDEMKDLWVTLRTEQLDWVDAFIDHQGHIAMANVLMNSIYKTAPRENLTKELLEKENSFFKCFRVLSMLSQGLYEFSTHRLMTDTVAEGLFSTKLATRKMATEIFVCMLEKKNKSRFEAVLTSLDKKFRIGQNLHMIQNFKKMPQYFSHLTLESHLKIIQAWLFAVEQTLDGRGKMGSLVGASDEFKNGGGENAILEYCQWTMVFINHLCSCSDNINQRMLLRTKLENCGILRIMNKIKLLDYDKVIDQIELYDNNKLDDFNVKLEANNKAFNVDLHDPLSLLKNLWDICKGTENEKLLVSLVQHLFLSSSKLIEENQNSSKLTKQLKLMDSLVTNVSVASTSDEETNMNMAIQRLYDAMQTDEVARRAILESRALTKKLEEIQAERDSLSEKLSKAEHGLVGQLEDELHERDRILAKNQRVMQQLEAELEELKKKHLLEKHQQEVELRKMLTILNSRPEESFNKNEGTRGMNSSLNSSEKANIQKVLQDGLSRAKKDYKDDSKKFGMTLQPNKRLKMLRMQMENIENEARQLEMTNFAEFEKDRLEPPIHIKKPKVKKMKNKDRKPLVKPQEADVNKLNDLRRALAEIQMESNDISKFNVEERVNELFNEKKSLALKRLKELETKYKGFGIDFNVDEIMDSPKKNTGDVETEEDANYASLDPKTYQKKLDEINRITDQLLDIQTQTEHEIQVEEDGESDLSSSSSDDESEEIYQDASPTQELRSEHSELSSGSGPGSFLDALSQKYGTGQNVTASAAFGENNNGSGIGPLHSKVEKTFMNRLRKSTVSSAPYLEELTQKVNKVEPYEQNEDEGLDKKSLPENSTASAASAFDKAEKDMRQHVENGKQGRVVNHEEDKTADFSAVSKLNNTDGAEDLSTQSSVLSSQPPPPPPPPPPVPAKLFGESLEKEKKSEDDTVKQETTGDSPAPPPPPPPPPPPPMALFGKPKGETPPPPPLPSVLSSSTDGVIPPAPPMMPASQIKSAVTSPLLPQSPSLFEKYPRPHKKLKQLHWEKLDCTDNSIWGTGKAEKFADDLYEKGVLADLEKAFAAREIKSLASKRKEDLQKITFLSRDISQQFGINLHMYSSLSVADLVKKILNCDRDFLQTPSVVEFLSKSEIIEVSVNLARNYAPYSTDWEGVRNLEDAKPPEKDPNDLQRADQIYLQLMVNLESYWGSRMRALTVVTSYEREYNELLAKLRKVDKAVSALQESDNLRNVFNVILAVGNFMNDTSKQAQGFKLSTLQRLTFIKDTTNSMTFLNYVEKIVRLNYPSFNDFLSELEPVLDVVKVSIEQLVNDCKDFSQSIVNVERSVEIGNLSDSSKFHPLDKVLIKTLPVLPEARKKGDLLEDEVKLTIMEFESLMHTYGEDSGDKFAKISFFKKFADFINEYKKAQAQNLAAEEEERLYIKHKKIVEEQQKRAQEKEKQKENSNSPSSEGNEEDEAEDRRAVMDKLLEQLKNAGPAKSDPSSARKRALVRKKYLSEKDNAPQLLNDLDTEEGSILYSPEAMDPTADTVIHAESPTPLATRGVMNTSEDLPSPSKTSALEDQEEISDRARMLLKELRGSDTPVKQNSILDEHLEKLRARKERSIGEASTGNRLSFK 0C5A9D5280A5858F 0C5A9D5280A5858F tba3_yeast Tubulin alpha-3 chain YM7056.02C YML124C TUB3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MREVISINVGQAGCQIGNACWELYSLEHGIKEDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDYIEVGADSYAEEF 189889B795E72448 189889B795E72448 num1_yeast Nuclear migration protein NUM1 NUM1 YDR150W PAC12 YD8358.06 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSHNNRHKKNNDKDSSAGQYANSIDNSLSQESVSTNGVTRMANLKADECGSGDEGDKTKRFSISSILSKRETKDVLPEFAGSSSHNGVLTANSSKDMNFTLELSENLLVECRKLQSSNEAKNEQIKSLKQIKESLSDKIEELTNQKKSFMKELDSTKDLNWDLESKLTNLSMECRQLKELKKKTEKSWNDEKESLKLLKTDLEILTLTKNGMENDLSSQKLHYDKEISELKERILDLNNENDRLLISVSDLTSEINSLQSNRTERIKIQKQLDDAKASISSLKRKVQKKYYQKQHTSDTTVTSDPDSEGTTSEEDIFDIVIEIDHMIETGPSVEDISEDLVKKYSEKNNMILLSNDSYKNLLQKSESASKPKDDELMTKEVAENLNMIALPNDDNYSKKEFSLESHIKYLEASGYKVLPLEEFENLNESLSNPSYNYLKEKLQALKKIPIDQSTFNLLKEPTIDFLLPLTSKIDCLIIPTKDYNDLFESVKNPSIEQMKKCLEAKNDLQSNICKWLEERNGCKWLSNDLYFSMVNKIETPSKQYLSDKAKEYDQVLIDTKALEGLKNPTIDFLREKASASDYLLLKKEDYVSPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELQRKYSELEKEVEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDVEFLKEKSAKLGHTVVSSEEYSELQRKYSELEKEVEQPSLAYLVEHAKATDHHLLSDSAYEELVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENSDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEELVKCKENPDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEELVKCKENPDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDVEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLAYLVEHAKATDHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNEAYSELEKKLEQPSLEYLVEHAKATNHHLLSDSAYEDLVKCKENPDMEFLKEKSAKLGHTVVSNKEYSELEKKLEQPSLEYLVKHAEQIQSKIISISDFNTLANPSMEDMASKLQKLEYQIVSNDEYIALKNTMEKPDVELLRSKLKGYHIIDTTTYNELVSNFNSPTLKFIEEKAKSKGYRLIEPNEYLDLNRIATTPSKEEIDNFCKQIGCYALDSKEYERLKNSLENPSKKFIEENAALLDLVLVDKTEYQAMKDNASNKKSLIPSTKALDFVTMPAPQLASAEKSSLQKRTLSDIENELKALGYVAIRKENLPNLEKPIVDNASKNDVLNLCSKFSLVPLSTEEYDNMRKEHTKILNILGDPSIDFLKEKCEKYQMLIISKHDYEEKQEAIENPGYEFILEKASALGYELVSEVELDRMKQMIDSPDIDYMQEKAARNEMVLLRNEEKEALQKKIEYPSLTFLIEKAAGMNKILVDQIEYDETIRKCNHPTRMELEESCHHLNLVLLDQNEYSTLREPLENRNVEDLINTLSKLNYIAIPNTIYQDLIGKYENPNFDYLKDSLNKMDYVAISRQDYELMVAKYEKPQLDYLKISSEKIDHIVVPLSEYNLMVTNYRNPSLSYLKEKAVLNNHILIKEDDYKNILAVSEHPTVIHLSEKASLLNKVLVDKDDFATMSRSIEKPTIDFLSTKALSMGKILVNESTHKRNEKLLSEPDSEFLTMKAKEQGLIIISEKEYSELRDQIDRPSLDVLKEKAAIFDSIIVENIEYQQLVNTTSPCPPITYEDLKVYAHQFGMELCLQKPNKLSGAERAERIDEQSINTTSSNSTTTSSMFTDALDDNIEELNRVELQNNEDYTDIISKSSTVKDATIFIPAYENIKNSAEKLGYKLVPFEKSNINLKNIEAPLFSKDNDDTSVASSIDLDHLSRKAEKYGMTLISDQEFEEYHILKDNAVNLNGGMEEMNNPLSENQNLAAKTTNTAQEGAFQNTVPHNDMDNEEVEYGPDDPTFTVRQLKKPAGDRNLILTSREKTLLSRDDNIMSQNEAVYGDDISDSFVDESQEIKNDVDIIKTQAMKYGMLCIPESNFVGASYASAQDMSDIVVLSASYYHNLMSPEDMKWNCVSNEELQAEVKKRGLQIALTTKEDKKGQATASKHEYVSHKLNNKTSTVSTKSGAKKGLAEAAATTAYEDSESHPQIEEQSHRTNHHKHHKRQQSLNSNSTSKTTHSSRNTPASRRDIVASFMSRAGSASRTASLQTLASLNEPSIIPALTQTVIGEYLFKYYPRLGPFGFESRHERFFWVHPYTLTLYWSASNPILENPANTKTKGVAILGVESVTDPNPYPTGLYHKSIVVTTETRTIKFTCPTRQRHNIWYNSLRYLLQRNMQGISLEDIADDPTDNMYSGKIFPLPGENTKSSSKRLSASRRSVSTRSLRHRVPQSRSFGNLR EB4E48F950621142 EB4E48F950621142 kar9_yeast Karyogamy protein KAR9 YPL269W KAR9 Cortical protein KAR9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNDGPRSMTIGDDFQENFCERLERIHNTLHSINDCNSLNESTTSISETLLVQFYDDLENVASVIPDLVNKKRLGKDDILLFMDWLLLKKYMLYQFISDVHNIEEGFAHLLDLLEDEFSKDDQDSDKYNRFSPMFDVIEESTQIKTQLEPWLTNLKELLDTSLEFNEISKDHMDTLHKIINSNISYCLEIQEERFASPIRHTPSFTLEQLVKLLGTHTETTEPKVPKFSPAEDILSRKFLNLKKNIPPIEKSLTDILPQRIVQFGHRNITNITTLQTILQKKYELIMKDYRFMNSEFRELKVELIDKRWNILFINLNHELLYILDEIERLQSKLLTTKYTKDITIRLERQLERKSKTVSKTFNIIYRALEFSLLDAGVASKTNELAQRWLNIKPTADKILIKSSASNKIATSKKKIPKPKSLGFGRPNSVIGTITQDFQERVAINEGDSNKTPENSTTVALKGKKLGKALLQKMNIKPATSPNSSNAINPFFDPESPNKGKLILSSVPPLPYDETDETTLRVSRGENEKSPDSFITSRHENKVQITETPLMAKNKSVLDIEKDKWNHYRSLPSRIPIYKDKVVKVTVENTPIAKVFQTPPTKITTPNSQVWVPSTRRRTRLRPPTPLSQLLSPREGRLDKTPTY F14D4D570AC2B5A3 pac11_yeast WD repeat-containing protein PAC11 PAC11 YDR488C protein protein yeast Saccharomyces cerevisiae Baker's yeast MERLKQLEEKRRQLKELRERRKQASLFPGSETMGHHPTEVHAKATMVSVSVQTDMEEGSKIQEPQSAYLRRKEVITYDKGIQTDQIEEEQLQENENHTTTDAVAIETTAADENNKDKAENDQPRLELAKPFLVEEAAATLSNASFARLETEVSASGQQAPSNMQQDKDNLMQWNMVSENLQSETDCDCIAQEYDPGKGVLVVVYLRLPPADLQYASSEAAWSVVNVVKCDNASGRNGLLIDMVEFRGTRIMTATILRRYHPESNVISILLATLTGKIILYELRLKQKKPETPVVYVVQRNMVARHYFQHPVVAVIETSSVQDQERVLVAADNGNIMELSCLDLTVLRKPQQLRPVPLSQLLSLENDTCTYTERLQRLAKFDEVGIACMAYTSEDPQYVWIGGEDGGIYKVFWDQPGPLYLSLDNNGFQPAENHSTRVTGLEFHWDDARRLMLLLSCSTDWTVRLWDARAGKAIIGAPLLLGGPVLRARWLEKNNGGENSRTLRCQVWCADGRLVVVNWAFDAKTSLYTATVIS 2037021799414E9A 2037021799414E9A num1-pac11 1 6 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 7A The interaction requires the presence of protein DYN1. num1-tub3 1 4 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 7B num1-tub3-1 1 4 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 7B num1-kar9 1 5 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 10 num1-bni1 1 2 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 10