UniProt UniProt http://www.uniprot.org Identification of Cox20p, a novel protein involved in the maturation and assembly of cytochrome oxidase subunit 2. Identification of Cox20p, a novel protein involved in the maturation and assembly of cytochrome oxidase subunit 2. J. Biol. Chem. (0021-9258) 2000 imex curation Hell K., Tzagoloff A., Neupert W., Stuart RA. The chemical cross-linker SPDP was added to isolaed mitochondria following the synthesis of mitochondrial translation products in the presence of 35S methionine. The cross-linked adducts were identified after coimmunoprecipitation using antiserum specific for COX20 or COX2. rosemary.stuart@marquette.edu 13-SEP-2006: Contacted by JYOTI Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Hell K., Tzagoloff A., Neupert W., Stuart RA. The chemical cross-linker SPDP was added to isolaed mitochondria following the synthesis of mitochondrial translation products in the presence of 35S methionine. The cross-linked adducts were identified after coimmunoprecipitation using antiserum specific for COX20 or COX2. rosemary.stuart@marquette.edu Accepted 2006-AUG-24 by JYOTI. 13-SEP-2006: Contacted by JYOTI J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation Identification of Cox20p, a novel protein involved in the maturation and assembly of cytochrome oxidase subunit 2. Identification of Cox20p, a novel protein involved in the maturation and assembly of cytochrome oxidase subunit 2. J. Biol. Chem. (0021-9258) 2000 imex curation Hell K., Tzagoloff A., Neupert W., Stuart RA. The chemical cross-linker SPDP was added to isolaed mitochondria following the synthesis of mitochondrial translation products in the presence of 35S methionine. The cross-linked adducts were identified after coimmunoprecipitation using antiserum specific for COX20 or COX2. rosemary.stuart@marquette.edu 13-SEP-2006: Contacted by JYOTI Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast crosslink cross-linking study crosslink predetermined predetermined participant predetermined Hell K., Tzagoloff A., Neupert W., Stuart RA. The chemical cross-linker SPDP was added to isolaed mitochondria following the synthesis of mitochondrial translation products in the presence of 35S methionine. The cross-linked adducts were identified after coimmunoprecipitation using antiserum specific for COX20 or COX2. rosemary.stuart@marquette.edu Accepted 2006-AUG-24 by JYOTI. 13-SEP-2006: Contacted by JYOTI J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation cox2_yeast Cytochrome c oxidase subunit 2 COX2 OXI1 Q0250 Cytochrome c oxidase polypeptide II protein protein yeast Saccharomyces cerevisiae Baker's yeast MLDLLRLQLTTFIMNDVPTPYACYFQDSATPNQEGILELHDNIMFYLLVILGLVSWMLYTIVMTYSKNPIAYKYIKHGQTIEVIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGYQWYWKYEYSDFINDSGETVEFESYVIPDELLEEGQLRLLDTDTSMVVPVDTHIRFVVTAADVIHDFAIPSLGIKVDATPGRLNQVSALIQREGVFYGACSELCGTGHANMPIKIEAVSLPKFLEWLNEQ BA5717C37330DF41 cox20_yeast Cytochrome c oxidase assembly protein COX20, mitochondrial COX20 YD9934.15c YDR231C protein protein yeast Saccharomyces cerevisiae Baker's yeast MRWWPWSNQTEDQKQQQQPQGKADGDRVLTNYSRGQKILLEDTPPKFADDLSNSQLAKKQERATLKEAWDSIRWSDFSLQKLTSIPCFRDAGMLGFSSMFLMGSIIFIYHKSPTKATNWAMSSLILGSIVGWEQCRLKRQKSFQIAQLAKETVAKKEKPMLHNVPHDPSLPGQWEAAKNEKQSQFEQSNQNLSQASSEKKWYKFW 1E1893856FF8456D cox2-cox20 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association No interaction was detected in mitochondria from strains deleted of OAX1, suggesting that the interaction occurs following OXA1-dependent export across the inner membrane 7A, 7B cox2-cox20-1 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 7C cox2-cox20-2 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association No interaction was detected in mitochondria from strains deleted of OAX1, suggesting that the interaction occurs following OXA1-dependent export across the inner membrane 7A, 7B