UniProt UniProt http://www.uniprot.org The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA (1355-8382) 1998 Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. solution sedimentati cosedimentation in solution solution sedimentati western blot western blot Immuno blot Immuno blot Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). RNA (1355-8382) 1998 The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA (1355-8382) 1998 Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip edman degradation edman degradation Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). RNA (1355-8382) 1998 The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA (1355-8382) 1998 Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). RNA (1355-8382) 1998 The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA (1355-8382) 1998 Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC. Monoclonal antibody 12E12 was used for immunoprecipitation of nucleoplasmic preparations. It recognizes the A6 epitope of U1A protein (amino acids 103-115) and binds to U1A only when the protein is part of a non-snRNP complex that is not associated with U1 RNA. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). RNA (1355-8382) 1998 snrpa_human U1 small nuclear ribonucleoprotein A SNRPA protein protein human Homo sapiens Human MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK DIP protein P09012 original sequence version: 99 9426E83EE5A22894 sfpq_human Splicing factor, proline- and glutamine-rich SFPQ PSF Polypyrimidine tract-binding protein-associated-splicing factor DNA-binding p52/p100 complex, 100 kDa subunit 100 kDa DNA-pairing protein protein protein human Homo sapiens Human MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF DIP protein P23246 original sequence version: 182 6D8D5EA95E235847 snrpa-sfpq-1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association snrpa-sfpq-2 1 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association snrpa-sfpq-3 3 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association snrpa-sfpq-4 4 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association