IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. J. Neurosci. (0270-6474) 1998 Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Drosophila head cDNA library used for screening. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Drosophila head cDNA library used for screening. J. Neurosci. (0270-6474) 1998 dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. J. Neurosci. (0270-6474) 1998 Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Drosophila head cDNA library used for screening. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Drosophila head cDNA library used for screening. J. Neurosci. (0270-6474) 1998 dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. J. Neurosci. (0270-6474) 1998 Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Drosophila head cDNA library used for screening. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Drosophila head cDNA library used for screening. J. Neurosci. (0270-6474) 1998 dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels. J. Neurosci. (0270-6474) 1998 Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Drosophila head cDNA library used for screening. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Xia X., Hirschberg B., Smolik S., Forte M., Adelman JP. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Drosophila head cDNA library used for screening. J. Neurosci. (0270-6474) 1998 slo_drome Calcium-activated potassium channel slowpoke Maxi K channel BK channel slo CG10693 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASGLIDTNFSSTLANGMSGCDQSTVESLADDPTDSPFDADDCLKVRKYWCFLLSSIFTFLAGLLVVLLWRAFAFVCCRKEPDLGPNDPKQKEQKASRNKQEFEGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFVDASSEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLDFNNAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSPDMQSWTNDYLRGTGMEMYTETLSPTFIGIPFAQATELCFSKLKLLLLAIEIKGAEEGADSKISINPRGAKIQANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKNLTVQPRSKFDDLDEHHPAPTFTPPELPKRVHVRGSVSGDITRDREDTNLLNRNVRRPNGTGNGTGGMHHMNNTAAAAAAAAAAGKQVNKVKPTVNVSRQVEGQVISPSQYNRPTSRSSGTGTQNQNGGVSLPAGIADDQSKDFDFEKTEMKYDSTGMFHWSPAKSLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSNDDPTLADKEAILASLNIKAMTFDDTIGVLSQRGPEFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTVESLSNRDRCRVGQISLYDGPLAQFGECGKYGDLFVAALKSYGMLCIGLYRFRDTSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQFDPGLEYKPPAVRAPAGGRGTNTQGSGVGGGGSNKDDNS 306A12234C63AD0D slip1_drome Slo-interacting protein 1 Slip1 dSLIP1 CG1783 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSIADVEYEYVVLKINGYDISHLSRYEAVQKFLQSKETLVVEIRRQKHNALDLELKHGSNAKISKVDNPGELSVLTDKSAEGTITAASASQQINCPSSTSLKEIETKTPVVLTLRARSHEDRLGSLQAASKETQTQSVVGTDVLKDNDLVNTITDNFIEHEHHLFEQCLEPEIDIEEVTLVKGVEQSSSNQIGLIVTSSGIQQSSTDTNKGDILGNVLEHSEDVFISGVQPESIAYRDGRLRQGDQILRINGLDVKNQEELETQIARSSTSVTLLVSRILYPEDDDDEDIHFEYANTFLPDDYTNVVDKLDKVLLTHVKSLEELSNKSAMQSDECYHIPEKNSSDSNVKISSLAKNIIEQSTKSCSKIKLRPNANLDYKKKFNLPLSQEEVHLQYEYDESEHIYETIPEDSESEPVYCSPYQRSNDKTSIGCSSPIASRPAESLERTMQQQTQRVAQWLGLKPQYQKTRQTLVGRPPPLKLVQQPTCSRVFTLRSTLTNTSASSSSGVAYSSYGQNNVVTGNAAAPGEEVDNSSSAYNTGDSNNSASPHQNTTNPDEAIATGRKLDSTVIDSPNDHLDATGVSTMLLLPFGKSGRIGLCSSNLPTAYVSERYTNVGSENEIHPLKSDIEILRVKPTDDSYSHCPQFNAPNLSSYHFVSSQEVANRCHISTSLQKNATLLNGESAEEIPMVWKVKRRPDGTRYIVKRPVRNRPQVALRKNMRYNEVTTTEDDTISEVKIGRYWTKEERKRHIERAREKRHHQTQQQQQ 8914206A7C34E46F slo-slip-1 2 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association slo-slip-2 2 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-538417]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 1164-1164, sequence length 767 [EBI-538417]665-1164 region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association slo-slip-5 1 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association slo-slip-4 3 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey c terminal binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-533109]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 774-774, sequence length 767 [EBI-533109]667-774 25 23 physical association physical association slo-slip 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait c terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association