UniProt UniProt http://www.uniprot.org A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell (0092-8674) 2005 Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2005 A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell (0092-8674) 2005 Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu xenla-oocyte Xenopus laevis oocyte Oocyte coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2005 A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell (0092-8674) 2005 Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2005 A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. Cell (0092-8674) 2005 Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu xenla-oocyte Xenopus laevis oocyte Oocyte coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Blower MD., Nachury M., Heald R., Weis K. fig 2a kweis@berkeley.edu Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Cell (0092-8674) 2005 if23a_xenla Insulin-like growth factor 2 mRNA-binding protein 3-A KH domain-containing transcription factor B3-A Vg1 localization element binding protein A Vg1 RNA-binding protein A RNA-binding protein Vera-A VLE-binding protein A VgLE-binding and ER association protein A 69 kDa RNA-binding protein A Trans-acting factor B3-A VICKZ family member 3-A IGF-II mRNA-binding protein 3-A igf2bp3-a vera-a vickz3-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MNKLYIGNLSENVSPTDLESLFKESKIPFTGQFLVKSGYAFVDCPDETWAMKAIDTLSGKVELHGKVIEVEHSVPKRQRSRKLQIRNIPPHLQWEVLDSLLAQYGTVENCEQVNTESETAVVNVTYANKEHARQGLEKLNGYQLENYSLKVTYIPDEMATPQAPSQQLQQQPQQQHPQGRRGFGQRGPARQGSPGAAARPKPQTEVPLRMLVPTQFVGAIIGKEGATIRNITKQTQSKIDIHRKENAGAAEKPITIHSTPEGCSAACKIIMEIMQKEAQDTKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQDLTLYNPERTITVKGSIEPCAKAEEEIMKKIRESYENDIAAMNLQAHLIPGLNLNALGLFPSSSSGMPPPSVGVPSPTSSTSYPPFGQQPESETVHLFIPALAVGAIIGKQGQHIKQLSRFAGASIKIAPAEGPDAKLRMVIITGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLETHIKVPSYAAGRVIGKGGKTVNELQNLTSAEVVVPRDQTPDENDEVVVKITGHFYASQLAQRKIQEILAQVRRQQQQQQKTVQSGQPQPRRK 54CEA7BFF0856DD6 q7zzy7_xenla Rae1 protein homolog rae1.S Gle2 Rae1 mRNA export factor mRNA-associated protein mrnp 41 XELAEV_18046216mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MTLFSTPGGFGTGGGSMFGSTATDNHNPMKDIEVASPPDDSISCLSFSPQTLPGNFLIAGSWANDVRCWEVQDNGQTIPKAQQMHTGPVQDVCWSDDGTKVFTASCDKTAKMWDLNSNQSIQIAQHDAPIKTVHWVKAPNYSCIMTGSWDKTLKFWDTRSPNPLLSIQLPERGYCADVVYPMAVVATAERGLIVYQLENQPSEFRRIDSPLKHQHRCVGIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTAAPQDIYAVNDIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPITACCFNHNGNIFAYSSSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK BFE42E2929C3C81E q6dcp4_xenla Nup98 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MFNKTFGSPFGTGNGAFGATSTFGQTTGFGTTPAAAFGSAGFGTNTTTGGLFGNTQTKPGSLFGANTFNQPATSSASSGFGFGASTGTTNSLFGSTNTGSSLFSTPSNAFAQTKPASFGNFGTSTSTGGLFGNTNTANPFSGTSASLFGASTFSSAPTGTTIKFNPQSGTDTMAKGGVTTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNPVGAPAGTGLFGTSAATSSAPTGIFGSTAANSFSFAGNKTTFGAAGTGAFAANTGGLFGQPTNPPAASLFNKPFGNAATTQSTGFSFGNTSTLGQAQTSTMGLFGANQATQAGGLFGATTNTNAAGAFGAGSSLFGQPNPAPFGGGSTLFGNKPAGFGTTTTTAPAFGTTTGGLFGNKATLTLGTNTNTSNFGFGANAAGTSLFGNKPATGTIGPSLGTSFGTALNPGQTSLFGGNQPKLTGTLGTGAFGGTGFNSTSTGLGFGAPQPAMATLTDPNASAAQQMFLQQQYNALRYSPFPDSPLFRNPISDPKKKEELLKPTNPAAQKAVLTPTHYKLTPRPAARVRPKALQNSGAAKSQLFDALDDDEPVLSSGVFMPKKSIKKLDLKYLKNSSSLFAQESKDVNEVVPPTPETQDRSVENHHADEKKKK BD50CEE0732297BC imb_xenla Importin subunit beta p97 Importin-90 Karyopherin subunit beta kpnb1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MELVTILEKTVSPDRNELEAAQKFLEQAAVENLPTFVVELSKVLANPANSQVARVAAGLQIKNPLTSRDPDVKAQYQQRWLAIDASARGEIKTYVLRTLGTESYRPSSASQCVAGIACAEITVNQWPQLIPQLVANVTDPNSTERMKESTLEAIGYICQDIDPEQLQHKSNEILTAIIQGMRKEEPSNNVRLAATNALLNSLEFTKANFDKESERHYIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITVEAMKNEIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPESCQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTVGRICELLPEAAINDVYLAPLLQCLIEGLGAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPSSYCLSSSFEVIVQKLLETTDRPDGHQNNLRSAAYEALMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQVESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGAEFLKYMEAFKPFLTIGLKNYAEYQVCLAAVGLVGDLCRALQSNILPFCDEMMQFLLENLGNENVHRSVKPQILSVFGDVALAIGGEFKKYLDVVLNTLQQASQAQVDKSDYDMVDYLNELREGCIEAYTGIIQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDSVVACGAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKTKTLATWATKELRKLKNQA 2F8FAACECFFA3AD9 q8ax85_xenla khsrp.S Khsrp khsrp protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSDYPGAQSGNRKDAFADAVQRARQIAAKIGGDVTGGVNNTPEFNFGGQKRQLEDGDMFGFPSPDQPECKKLATQPESMPPQLAPVHPPRSSSMTEEYRVPDGMVGLIIGRGGEQINKIQQESGCKVQISPDSGGMPERIVSLTGNPDAVQKAKMLLDDIVLRGRGGPPSQFHDSSNGQNGSLQEIMIPAGKAGLIIGKGGETIKQLQERAGVKMILIQDGSQNTNMDKPLRIVGEPFKVQQACEMVMDLLKERDQPNFDRNEYGTRGGGGGGGGGGGIDVPVPRHSVGVVIGRSGDMIKKIQNDAGVRIQFKQDDGTGPDKIAHIMGPPDRCEHAASIISDLLQSLRTGPPGPPGPGMPPGGRGRGRGQGPWGPPGGEMTFSIPTHKCGLVIGRGGENVKAINQQTGAFVEISRQPPPNGDPNFKMFIIRGNPQQIDHAKQLIEEKIEGPLCPIGPGPPGPGPAGPMGPYNPGPYQPGPPGGPPHQGPPPPAAHQYPPQGWGSTYPQWGQPPAAHDPTKPPAPTDPSAAWAAYYSHYYQQPSAPVPGQPPAVPAPPPQGEPPQQPPSANQPDYTKAWEEYYKKMGQQTPQPAGQPDYTKAWEEYYKKQAATPGAPAAAVAAAAATAAPPAAQPDYSAAWAEYYRQQAAYYGQAPGAPPTQPPSAQPGPQAQ F9F7D5F5DE9B0F72 l14aa_xenla Protein LSM14 homolog A-A lsm14a-a lsm14a rap55a-a RNA-associated protein 55A-A protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPIPPRDEIFEYIIFRGSDIKDLTVCEPPKPQCSLPQDPAIVQSSLGSSSASSFQSVSSYGPFGRMPAYSQFNTGPLVGPQFGAVGVGSSLTSFGAETTSSTSLPPSSAVGTSFTQEARTLKTQSSQGQSSSPLDSLRKSPNIEQAVQTAAAPHAPSTATVGRRSPVLSRPVPSSIQKTAESPEQRKGELHKMQRPDIDQLKNDKNDPSKRQPVLSALQPRRGRGGNRGGRGRFGVRRDGPMKFEKDFDFESANAQFNKEEIDREFHNKLKIKDDKPEKPVNGEDKTDSVVDTQNSEGNAEEEEVLAGGVCYYDKTKSFFDNISCDDNRDRRQTWAEERRINVETFGLPLRSNRGRGGFRGRGGGMGFRGGRGRGGERRGAPGGGGFGPARGFRGGFRGGRGGREFADYEYRKDNKVAA 67A85C7E2FE93398 ilf3a_xenla Interleukin enhancer-binding factor 3-A ilf3-a ilf3 ubp3 Double-stranded RNA-binding protein 4F.1 CCAAT box transcription factor subunit protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRPMRIFLNDDRHVMAKHSVVYPTQEELEAVQNMVSHTERALKAVSDWIDQQEKDCSGEQEQPMAEETETTEEGKDSEMKTGENPTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCRDKPTISLLKRVADNLVLQFETVSEDKYEVIQNIREALIVVKSTKEPPLTLNIRLTSPLVREEMEKLSAGETLTVSDPPDVLDRHKCLAALASLRHAKWFQARANGLKSCVIVIRVLRDLCTRVPTWEPLRGWPLELLCEKAIGTANRPMGAGEALRRVLECLSSGILMPDGSGLYDPCEKDASDALEHLERQQREDITQSAQHALRLAAFGQLHKVLGMDPLPSKMPKKTKVETPVIDYTVQIPPSTTYAMPPLKRPIEEDGDDKSPSKKKKKIQKKDEKSEPPQVMNALMRLNQLKPGLQYKLISQTGPVHAPVFTMSVEVDDKTFEASGPSKKTAKLHVAVKVLQDMGLPTGMEEKEEGTDESEQKPVVQTPAQPDDSAEVDSAALDQAESAKQQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVEVDGVKFQGSGSNKKVAKAYAALSALEKLFPDYTMYTEAPKKKRPPMMPRGGPKFAGKHNQGFGMMYSEVPPPQAMRGRGRGGMNRGRGRGRGGFGGGYMNSGGYGGGYGGNYNYQTSATAGYSQFYSNGGASGNAGGGGAGTGGYSSYYQGDSYSAPTPPKPFVNKKPPPPQQQQQQQPPPQHASNPPKPSYNQGYQGHQGGQQQQQQQQQQQTYNQNQYSNYGPPQKQKGGYNQGAQGAGSGGSYNYSNSYTGGTAPLGTAVERVQEGEAAALTQRQALVTTQAHTPAMVEPAVLRRTKVTRSLITIKVPPDRTTVALQIITSSLREELGVTAGIQNTT 1AD98A9488345C85 tacc3_xenla Transforming acidic coiled-coil-containing protein 3 Cytoplasmic polyadenylation element-binding protein-associated factor Maskin tacc3 maskin protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSLQIINDENAGNDITAEKFDLLLNPQTTGRPSILRPSQKDNLPPKPALKSTKVTFQTPMRDPQTLRIMTPSVANKPENVFLLEDCTQALEQLHLSLPSSCGPNPVEINVSVSNNQPDSEELPVRTTGAYSIDFDNFDDINPFKSKTQMLNSPIKADLPSLTENIETTTPVVPADEASKQMVSLNLSAANLDSPVTVQFSSESGSIGVSEKTALDDTLPLSESGIKDLQGLNASSNNIEEPVPLDKDICSNNEKDDATMADSTCEGTSDAHTSSNNIEEPVTLDKDICSSNEKDNAAVADSTCEGTSDAQSPLPIPKSSYSFDPDQFDMMNPFKTGGSKLQNSPAGKKQTPPSADLNTAKTEPVKLEFNFGDGDVSERKPPPKKLGKRPLLKTAAKKPSPKPEIASEKQEQQTAKPSEDEAIVPKASYKFDWEKFDDPNFNPFGCGGSKISSSPKGQKIANEQPSACTQGSKPEAECTASDMAPAENADEKDHGEIEPSQDSGAAEDRSQAEDQSVALSKVEVPHEQTTDCSPVENETQPEVSLINEEPSQKEVEHTSSDMTPPEINGTDSEFKLATEADFLLAADMDFKPASEIFSEGFRQPVEIDYLENFGTNSFKESVLRKQSLYLKFDPLLRESPKKSAAGINLLPSVPLKCSSDLFGAIPEANFPLIPSIENEEKPKGLDLLGTFTVADTALLIVDAPSSVAVPNPFLSTSDAIVEMLKYSQKDMDAAIEAVRLEVQEKDLEVLEWKTKHEKLYLEYVEMGKIIAEFEGTITQILEDSQRQKETAKLELNKVLQEKQQVQVDLNSMETSFSELFKRLEKQKEALEGYRKNEEALKKCVEDYLVRIKKEEQRYQALKAHAEEKLNRANEEIAHVRSKAKSEATALQATLRKEQMKIQSLERSLEQKSKENDELTKICDDFILKMEKI DIP protein Q9PTG8 original sequence version: 30 B0BF1F2AA4901439 kpnb1-rae1-1 1 6 unspecified role unspecified role prey prey xenla-oocyte Xenopus laevis oocyte Oocyte 8 unspecified role unspecified role bait bait n-terminal s-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-618948]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 601-601, sequence length 260 [EBI-618948]71-601 n-teminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association By RanQ69L-GTP. rae1-kpnb1-1 4 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association By RanQ69L-GTP. kpnb1-rae1-2 3 8 unspecified role unspecified role bait bait n-terminal s-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction By GST-Nup98. rae1-kpnb1-2 2 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association RNAse treatment of this complex abrogates mitotic spindle organizing activity. Other uncharacterized subunits may exist.