IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. J. Biol. Chem. (0021-9258) 2005 Diaz-Moreno I., Diaz-Quintana A., De la Rosa MA., Ubbink M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species m.ubbink@chem.leidenuniv.nl in vitro In vitro saturation binding saturation binding predetermined predetermined participant predetermined Diaz-Moreno I., Diaz-Quintana A., De la Rosa MA., Ubbink M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species The copper in plastocyanine was replaced by the redox inactive cadmium to allow titration studies with both oxidized and reduced Cytochrome f without interference from electron transfer. m.ubbink@chem.leidenuniv.nl Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Biol. Chem. (0021-9258) 2005 Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. Structure of the complex between plastocyanin and cytochrome f from the cyanobacterium Nostoc sp. J. Biol. Chem. (0021-9258) 2005 Diaz-Moreno I., Diaz-Quintana A., De la Rosa MA., Ubbink M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species m.ubbink@chem.leidenuniv.nl in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Diaz-Moreno I., Diaz-Quintana A., De la Rosa MA., Ubbink M. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species m.ubbink@chem.leidenuniv.nl Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Biol. Chem. (0021-9258) 2005 plas_nosso Plastocyanin petE protein protein anaso Anabaena sp. (strain PCC 7119) MKLIAASLRRLSLAVLTVLLVVSSFAVFTPSASAETYTVKLGSDKGLLVFEPAKLTIKPGDTVEFLNNKVPPHNVVFDAALNPAKSADLAKSLSHKQLLMSPGQSTSTTFPADAPAGEYTFYCEPHRGAGMVGKITVAG 17BE99202F8B796E q6h8m0_nosso Cytochrome f petA protein protein anaso Anabaena sp. (strain PCC 7119) MRNACTRARLTRTARAMVKTLFIAIASVTFFFTSDLALPQSAAAYPFWAQQTYPETPREPTGRIVCANCHLAAKPTEVEVPQSVLPDTVFKAVVKIPYDTSVQQVGADGSKVGLNVGAVLMLPEGFKIAPEDRIPEELKEEIGDVYFQPYGEDKDNIVIVGPLPGEQYQEIVFPVLSPNPANDKNIHFGKYSVHVGGNRGRGQVYPTGEKSNNNLYSAAATGTISKIAKQEGEDGSVKYLVDIKTESGEVVSDTIPAGPELIVSEGQAVTAGDALTNNPNVGGFGQLDAEIVLQDANRVGWLIAFVALVMLAQVMLVLKKKQVEKVQAAEMNF 523055089993C728 pete-peta-1 2 3 unspecified role unspecified role neutral component neutral component plastocyanin sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain val70 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain leu48 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pro71 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pro72 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain leu98 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain met100 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pro102 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pro125 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his126 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ala129 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lys69 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain lys96 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gln97 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain glu124 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component n-terminus cyt f sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tyr45 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain phe47 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain tyr146 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pro162 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain pro164 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 23 7 direct interaction direct interaction stoichiometry 1:1 The binding interface involves the hydrophobic areas close to the metal centers in both proteins. The buried surface area is 1100-1200 Angstroms. Prolines are unusually abondant in this protein complex interface. The inability of proline to form hydrogen bonds may be employed in a way to limit the affinity of transient protein complex. pete-peta-2 1 3 unspecified role unspecified role neutral component neutral component plastocyanin sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component n-terminus cyt f sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction stoichiometry 1:1 The binding constant is 1.6 10E3 M-1 The affinity is independent of the oxidation state of cytochrome f