MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. FEBS Lett. (0014-5793) 2005 imex curation Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. peitanghuang@yahoo.com.cn, lubaisong@yahoo.com 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA Only protein-protein interactions mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. no myc-tagged proteins labelled using fluorescent secondary Abs. peitanghuang@yahoo.com.cn, lubaisong@yahoo.com Accepted 2011-AUG-25 by MGT 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. FEBS Lett. (0014-5793) 2005 imex curation Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. peitanghuang@yahoo.com.cn, lubaisong@yahoo.com 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. peitanghuang@yahoo.com.cn, lubaisong@yahoo.com Accepted 2011-AUG-25 by MGT 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. FEBS Lett. (0014-5793) 2005 imex curation Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. peitanghuang@yahoo.com.cn, lubaisong@yahoo.com 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. peitanghuang@yahoo.com.cn, lubaisong@yahoo.com Accepted 2011-AUG-25 by MGT 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. Yeast two-hybrid screens imply that GGNBP1, GGNBP2 and OAZ3 are potential interaction partners of testicular germ cell-specific protein GGN1. FEBS Lett. (0014-5793) 2005 imex curation Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. peitanghuang@yahoo.com.cn, lubaisong@yahoo.com 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang J., Wang Y., Zhou Y., Cao Z., Huang P., Lu B. no peitanghuang@yahoo.com.cn, lubaisong@yahoo.com Accepted 2011-AUG-25 by MGT 23-Sept-2011: Contacted by JYOTI. Mouse testis cDNA FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation ggnb1_mouse Gametogenetin-binding protein 1 Ggnbp1 protein protein mouse Mus musculus Mouse MAAPARTPRSRILGCSSMLRFLRNLVGSKGGSKSTNKPLTRSQPSSSWEQDVVSPMMGHQGGRGRKEPRAKVHSAASSNGKREPPPRVLSAAPSNPRHDAFELGTGDSGSQTLTSKDVPKLRAQGVEVTSVPLRGTWEVLEQLPEKKGEEEEPVGEVSGASDREHFGQALETEQGCLQWVPGPLALTPGAFIKEEEDEHCPIEFGDLKPSSCKVGSTPWNYLLGLYKQLQKSAMAKAQRPAAPQLALKDGLPHEEKGEREEAVDESCPKWCAPRASSDESCPKWCAPRASTYQSPLQKKFRSTDTVGFVESELKKILSVQREARLWKVGNPEGRELLTQPDITLEEAGMVDGQHLLLEEMDEMGNWPPPD ggnb2_mouse Gametogenetin-binding protein 2 Ggnbp2 Zfp403 Znf403 Dioxin-inducible factor 3 Protein ZNF403 protein protein mouse Mus musculus Mouse MARLVAVCRDGEEEFPFERRQIPLYIDDTLTMVMEFPDNVLNLDGHQNNGAQLKQFIQRHSMLKQQDLSIAMVVTSREVLSALSQLVPCVGCRRSVERLFSQLVESGNPALEPLTVGPKGVLSLTRSCMTDAKKLYTLFYVHGSKLNDMIDAIPKSKKNKRCQLHSLDTHKPKPLGGCWMDVWELMSQECRDEVVLIDSSCLLETLETYLRKHRFCTDCKNKVLRAYNILIGELDCSKEKGYCAALYEGLRCCPHERHIHVCCETDFIAHLLGRAEPEFAGGRRERHAKTIDIAQEEVLTCLGIHLYERLHRIWQKLRAEEQTWQMLFYLGVDALRKSFEMTVEKVQGISRLEQLCEEFSEEERVRELKQEKKRQKRKNRRKNKCVCDTPASLHTADEKAVSREKETDFIENSCNACGSAEDGETCVEVMVTSENTSCTCPSSGNLLGSPKIKKGMSPHCNGSDCGYSSSMEGSETGSREGSDVACTEGICNHDEHGEDSCVHHCEDKEDDGDSCVECWANSEENNIKGKNKKKKKKSKMLKCDEHIQKLGSCITDPGNRETSGNTMHTVFHRDKTKDAHPESCCSTEKGGQPLPWFEHRKSVPQFTEPTEMSFGPDSGKGAKSLVELLDESECTSDEEIFISQDEIQSFMANNQSFYSNREQYRQHLKEKFNKYCRLNDHKRPVCSGWLTTAGAN C4F3976169B8646C oaz3_mouse Ornithine decarboxylase antizyme 3 Oaz3 OAZ-t protein protein mouse Mus musculus Mouse MPCNRSRPSLYSLSYIKRGKTRNYLYPFWSPFAYYLYCYKYRITLREKMLPCCYKSITYKEQEDLTLRPHCCLPCSCLPCSCLQCSESLGGLQVGRSTAQEKDHSQLKELYSAGNLTVLSTDPLLHQDPVQLDFHFRLTPHSSAHWHGLLCDHRLFLDIPYQALDQGNRESLTATLEYVEEKTNVDSVFVNFQIDRKDRGALLRAFSYMGFEVVRPDHPALPPWDNVIFMVYPLERDLGHPGQ 146EAE4F9C465FD6 ggn_mouse Gametogenetin Ggn protein protein mouse Mus musculus Mouse MGNVQSEPSAGGGSRKEQASDRASDSRRTPLVEPEVTPSSPAMRLARGLGVWFPGSSGPPGLLIPPEPQASSSPLPLTLELPSPVTPPPEEAAAVSTPPPPPVGTLLPAPSKWRKPTGTSVPRIRGLLEASHRGQGDPPSLRPLPPLPRQLTEKDPVLRAPAPPPTPLEPRKQLPPAPSTCDPQPLSRRITLASSATSPTESQVRHSSEGQAAGGAHGGVPPQAGEGEMARSATSESGLSLLCKVTFKSGPHLSPTSASGPLAAKASPGAGGGGLFASSGAISYAEVLKQGPQPPGATRPLGEVPPGATRPLGEVPRAAQETEGGDGDGEGCSGPPSVPAPLARALPPPPYTTFPGSKPKFDWVSPPDGTERHFRFNGAVGGIGAPRRRTTTLSGPWGSPPPRSGQTHPSSGPRRPTPALLAPPMFIFPAPNNGEPVRPVPPSPQQIPPLPPPPPTPPATPPPAPPPTPQPPALPRTPILVARPPTPGPGHLESALAPTPPSTLSPTAAADQVPAATPATVTSQVPATATAELSPPMPQPKTRTRRNKGPRAARGVIREEGTSGDGPREPNTAPVTDSSSGGGGGGSNGTSTAGASNKGTARHWPPFEVLNSCPCKCYCRHQRRHRRLPRNVSAWLSTPTNHLSEPPWVATVKLAGSLVAGLEHYDLQATHST ggn-ggnb1 2 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Fig. 4 ggnb1-ggn-1 3 5 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 17 physical association physical association Fig. 1 ggn-ggnbp2-3 4 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 7A ggn-ggnbp2 2 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 4 ggn-oaz3 2 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association Fig. 4 ggn-ggnbp2-1 3 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 34 36 physical association physical association Fig 2 ggnbp2-ggn 3 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2 ggn-oaz3-1 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3 ggn-ggnbp1 1 5 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Fig. 6A-C, J-L ggn-ggnbp2-2 1 6 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 6D-F, M-O ggn-oaz3-3 1 7 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig.6 G-I, P-R ggnbp2-ggn-1 4 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 7A oaz3-ggn 4 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 7B ggn-oaz3-4 4 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 7B