UniProt UniProt http://www.uniprot.org Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes. Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes. J. Cell Biol. (0021-9525) 2004 Luhtala N., Odorizzi G. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Luhtala N., Odorizzi G. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Cell Biol. (0021-9525) 2004 bro1_yeast Vacuolar-sorting protein BRO1 ASI6 NPI3 VPS31 YPL084W BRO1 LPF2 BRO domain-containing protein 1 BCK1-like resistance to osmotic shock protein 1 Vacuolar protein-sorting-associated protein 31 Amino acid sensor-independent protein 6 Nitrogen permease inactivating protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKPYLFDLKLKDTEKLDWKKGLSSYLKKSYGSSQWRTFYDEKATSELDHLRNNANGELAPSSLSEQNLKYYSFLEHLYFRLGSKGSRLKMDFTWYDAEYSSAQKGLKYTQHTLAFEKSCTLFNIAVIFTQIARENINEDYKNSIANLTKAFSCFEYLSENFLNSPSVDLQSENTRFLANICHAEAQELFVLKLLNDQISSKQYTLISKLSRATCNLFQKCHDFMKEIDDDVAIYGEPKWKTTVTCKLHFYKSLSAYYHGLHLEEENRVGEAIAFLDFSMQQLISSLPFKTWLVEFIDFDGFKETLEKKQKELIKDNDFIYHESVPAVVQVDSIKALDAIKSPTWEKILEPYMQDVANKCDSLYRGIIPLDVYEKESIYSEEKATLLRKQVEETETANLEYSSFIEFTNLPRLLSDLEKQFSDGNIFSNTDTQGQLMRDQIQTWCKFIQTNEFRDIEEQMNKIVFKRKQILEILSALPNDQKENVTKLKSSLVAASNSDEKLFACVKPHIVEINLLNDNGKIWKKFDEFNRNTPPQPSLLDIDDTKNDKILELLKQVKGHAEDLRTLKEERSRNLSELRDEINNDDITKLLIINKGKSDVELKDLFEVELEKFEPLSTRIEATIYKQSSMIDDIKAKLDEIFHLSNFKDKSSGEEKFLEDRKNFFDKLQEAVKSFSIFASDLPKGIEFYDSLFNMSRDLAERVRVAKQTEDSTANSPAPPLPPLDSKASVVGGPPLLPQKSAAFQSLSRQGLNLGDQFQNLKISAGSDLPQGPGIPPRTYEASPYAATPTMAAPPVPPKQSQEDMYDLRRRKAVENEERELQENPTSFYNRPSVFDENMYSKYSS 34DE0CDD2F4AE55E ubp4_yeast Ubiquitin carboxyl-terminal hydrolase 4 DOA4 MUT4 SSV7 YDR069C YD9609.23C YD8554.02C D4270 NPI2 Ubiquitin-specific-processing protease 4 Deubiquitinating enzyme 4 Vacuole biogenesis protein SSV7 Ubiquitin thioesterase 4 DOS1 UBP4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEQNIISTIRDECIRHRSKYLTIAQLTAIAEAKINEFIITGKAKDQDLSSLLDKCIDILSIYKKNSKDIKNIISCKNKGAMISSNSVMIIQLNYVYYKVIHIIVTTNIPHLSEFAKIKLHKSTSDEGNGNNNNNEFQLMNIYNTLLETLLKDENIAKIKSFIKSSIKQTKLNHEQEECNLMRTGSYITSNQLNSLISSSANSASSQMEILLIDIRSRLEFNKSHIDTKNIICLEPISFKMSYSDHDLEKKSLITSPNSEIKMFQSRNLFKFIILYTDANEYNVKQQSVLLDILVNHSFEKPISDDFTKIFILESGFPGWLKSNYGRQVSSSFPSNNNIKDDSVYINGNTSGLSLQHLPKMSPSIRHSMDDSMKEMLVAPTPLNHLQQQQQQQSDNDHVLKRSSSFKKLFSNYTSPNPKNSNSNLYSISSLSISSSPSPLPLHSPDPVKGNSLPINYPETPHLWKNSETDFMTNQREQLNHNSFAHIAPINTKAITSPSRTATPKLQRFPQTISMNLNMNSNGHSSATSTIQPSCLSLSNNDSLDHTDVTPTSSHNYDLDFAVGLENLGNSCYMNCIIQCILGTHELTQIFLDDSYAKHININSKLGSKGILAKYFARLVHMMYKEQVDGSKKISISPIKFKLACGSVNSLFKTASQQDCQEFCQFLLDGLHEDLNQCGSNPPLKELSQEAEARREKLSLRIASSIEWERFLTTDFSVIVDLFQGQYASRLKCKVCSHTSTTYQPFTVLSIPIPKKNSRNNITIEDCFREFTKCENLEVDEQWLCPHCEKRQPSTKQLTITRLPRNLIVHLKRFDNLLNKNNDFVIYPFLLDLTPFWANDFDGVFPPGVNDDELPIRGQIPPFKYELYGVACHFGTLYGGHYTAYVKKGLKKGWLYFDDTKYKPVKNKADAINSNAYVLFYHRVYGV B863FE6062510F3C bro1-doa4 1 3 unspecified role unspecified role prey prey doa4-gfp C-terminus GFP tagged protein Doa4 tag tag certain certain sequence position certain certain certain sequence position certain 2 unspecified role unspecified role bait bait c-terminus C-terminus HA tagged protein Bro1 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag certain certain sequence position certain certain certain sequence position certain physical association physical association bro1-doa4-1 1 2 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged protein Bro1 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait doa4-gfp C-terminus GFP tagged protein Doa4 tag tag certain certain sequence position certain certain certain sequence position certain physical association physical association bro1-doa4-2 1 2 unspecified role unspecified role prey prey c-terminus C-terminus HA tagged protein Bro1 ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait doa4-gfp C-terminus GFP tagged protein Doa4 tag tag certain certain sequence position certain certain certain sequence position certain physical association physical association Cell lysates were centrifuged before immunoprecipitation to show that the interaction occurs at the membrane level, especially when protein vps4 is absent.