IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular surface of tarantula toxins interacting with voltage sensors in K(v) channels. Molecular surface of tarantula toxins interacting with voltage sensors in K(v) channels. J. Gen. Physiol. (0022-1295) 2004 Wang JM., Roh SH., Kim S., Lee CW., Kim JI., Swartz KJ. The inhibitory activity of the toxin was examined against a mutant delat7 channel (to enable to subtract background conductance) in Xenopus oocyte using two-electrode voltage-clamp. The toxin was added to the recording chamber. xenla-oocyte Xenopus laevis oocyte Oocyte biochemical biochemical predetermined predetermined participant predetermined Wang JM., Roh SH., Kim S., Lee CW., Kim JI., Swartz KJ. The inhibitory activity of the toxin was examined against a mutant delat7 channel (to enable to subtract background conductance) in Xenopus oocyte using two-electrode voltage-clamp. The toxin was added to the recording chamber. Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). J. Gen. Physiol. (0022-1295) 2004 tx1_strgf Kappa-theraphotoxin-Scg1a SGTx1 SgTx-I protein protein strgf Stromatopelma calceatum griseipes (Feather leg baboon tarantula) Scodra griseipes TCRYLFGGCKTTADCCKHLACRSDGKYCAWDGTF 31B44D44BAF814A4 kcnb1_human Potassium voltage-gated channel subfamily B member 1 Voltage-gated potassium channel subunit Kv2.1 KCNB1 Delayed rectifier potassium channel 1 protein protein human Homo sapiens Human MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLEVCDDYSLDDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGENMGKKDKVQDNHLSPNKWKWTKRTLSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLEDMYNKMAKTQSQPILNTKESAAQSKPKEELEMESIPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLTSLPSKTGGSTAPEVGWRGALGASGGRFVEANPSPDASQHSSFFIESPKSSMKTNNPLKLRALKVNFMEGDPSPLLPVLGMYHDPLRNRGSAAAAVAGLECATLLDKAVLSPESSIYTTASAKTPPRSPEKHTAIAFNFEAGVHQYIDADTDDEGQLLYSVDSSPPKSLPGSTSPKFSTGTRSEKNHFESSPLPTSPKFLRQNCIYSTEALTGKGPSGQEKCKLENHISPDVRVLPGGGAHGSTRDQSI C4B426174ED0DEE4 tx1-kcnb1-1 1 2 unspecified role unspecified role neutral component neutral component P56855:p.Arg3Ala DDG>3.0, kd >5.10-4 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:09:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg3ala' Tue Jun 30 16:24:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg3Ala' P56855:p.Leu5Ala DDG>3.0, kd>5.10-4 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 19:12:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu5ala' Tue Jun 30 17:16:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu5Ala' P56855:p.Phe6Ala DDG>3.4, kd>10-3 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:13:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe6ala' Tue Jun 30 19:43:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe6Ala' P56855:p.Arg22Ala DDG>3.0, kd>5.10-4 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:12:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg22ala' Tue Jun 30 15:52:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg22Ala' P56855:p.Trp30Ala DDG=3.4, kd>10-3 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A Thu Jul 07 12:15:44 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was trp30ala Wed May 02 16:17:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp30ala' Tue Jun 30 16:41:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp30Ala' P56855:p.Asp31Ala DDG=1.4, Kd=3.10-5 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A Thu Jul 07 12:15:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was asp31ala Wed May 02 15:19:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp31ala' Tue Jun 30 19:16:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp31Ala' in vitro In vitro 3 unspecified role unspecified role neutral component neutral component xenla-oocyte Xenopus laevis oocyte Oocyte physical association physical association Kd for the following mutants is : kd>10-3 for W24A, kd >5.10-4 for R3A, L5A, R22A, kd= Kd=3.10-5 for D31A. Kd values were obtained by fitting a four site model.