IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). J. Biol. Chem. (0021-9258) 2002 Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu J. Biol. Chem. (0021-9258) 2002 Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). J. Biol. Chem. (0021-9258) 2002 Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu J. Biol. Chem. (0021-9258) 2002 Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). J. Biol. Chem. (0021-9258) 2002 Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu J. Biol. Chem. (0021-9258) 2002 Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). J. Biol. Chem. (0021-9258) 2002 Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu in vitro In vitro pull down pull down monoclonal western monoclonal antibody western blot monoclonal western Piechotta K., Lu J., Delpire E. Library -mouse brain 1A eric.delpire@vanderbilt.edu J. Biol. Chem. (0021-9258) 2002 s12a2_mouse Solute carrier family 12 member 2 Basolateral Na-K-Cl symporter Slc12a2 Nkcc1 Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 protein protein mouse Mus musculus Mouse MEPGPARPRLAPAARPGWGRAAGCRRRGGPARHGRASGQEDATTAGRQAGGGVRGEGTPAAGDGLGRPLGPTPSQSRFQVDPVSENAGRAAAAAAAAAAAAAAAGAAGKETPAAGKAGGESGVAKGSEEAKGRFRVNFVDPAASSSADDSLSDAAGVGGDGPNVSFQNGGDTVLSEGSSLHSGGGSGHHQQYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPVLLVRGNHQSVLTFYS 4DE24CC8070D42F2 stk39_mouse STE20/SPS1-related proline-alanine-rich protein kinase Serine/threonine-protein kinase 39 Stk39 Spak protein protein mouse Mus musculus Mouse MAEPSGSPVHVQLSQQAAPVTAAAATAPAAATSAPAPAPAPAPAASAAPAPAPAAAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAASQEKSRRVKEENSEISVNAGGIPEQIQSLSVHDSQAQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS DIP protein Q9Z1W9 original sequence version: 77 66085A90554311D8 q924n4-2 Solute carrier family 12 member 6 KCC3b Slc12a6 Kcc3 Electroneutral potassium-chloride cotransporter 3 K-Cl cotransporter 3 protein protein mouse Mus musculus Mouse MPHFTVTKVEDPEEGAAGPLSPEPSSAEVKARIQDPQEPDPSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS Produced by alternative promoter usage. 8CEA6F2DC4103DB2 oxsr1_mouse Serine/threonine-protein kinase OSR1 Oxidative stress-responsive 1 protein Oxsr1 Osr1 protein protein mouse Mus musculus Mouse MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKERVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEPTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKILQRAPTISERSKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGRAAISQLRSPRVKDSLSSSELFAAAEPMGTLLQVPEQISAHLPQPAGQMPTQPAQVSLLPPAEPAKPAQAQSSGERSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS 72A09F0D732375E1 q924n4-1 Solute carrier family 12 member 6 KCC3a Slc12a6 Kcc3 Electroneutral potassium-chloride cotransporter 3 K-Cl cotransporter 3 protein protein mouse Mus musculus Mouse MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSLATVALDPSSDRTSNPQDVTEDPSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS 74287FECDBC473BC s12a1_human Solute carrier family 12 member 1 Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 Kidney-specific Na-K-Cl symporter SLC12A1 NKCC2 protein protein human Homo sapiens Human MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGGIRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS C1C868B7F8B563AC slc12a6-stk39-1 3 9 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis range range certain certain sequence position certain physical association physical association slc12a6-stk39-2 2 9 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-621026]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 557-557, sequence length 556 [EBI-621026]461-557 physical association physical association slc12a6-oxsr1-2 2 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association slc12a6-stk39-5 2 7 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association slc12a2-stk39-1 2 5 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association slc12a1-stk39-1 2 10 unspecified role unspecified role bait bait Q13621:p.Arg20Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:54:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg20ala' Tue Jun 30 18:20:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg20Ala' Q13621:p.Phe21Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 19:41:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe21ala' Tue Jun 30 18:22:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe21Ala' Q13621:p.Val23Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 17:18:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val23ala' Wed May 02 17:38:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val23ala' Tue Jun 30 18:30:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val23Ala' Q13621:p.Ser24Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:34:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser24ala' Tue Jun 30 17:26:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser24Ala' Q13621:p.Val25Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 18:32:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val25ala' Tue Jun 30 19:08:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val25Ala' Q13621:p.Asn27Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 14:50:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn27ala' Tue Jun 30 19:05:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn27Ala' Q13621:p.Glu28Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:14:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu28ala' Tue Jun 30 19:01:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu28Ala' n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association Sequence of S12a1 given in paper conflicts with that in entry at positions 19 and 24. slc12a6-oxsr1-1 2 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait Q924N4-1:p.Arg14Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:36:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg14ala' Tue Jun 30 17:30:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg14Ala' Q924N4-1:p.Phe15Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 19:43:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe15ala' Tue Jun 30 18:24:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe15Ala' Q924N4-1:p.Val17Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 17:09:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val17ala' Tue Jun 30 17:26:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val17Ala' Q924N4-1:p.Pro19Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A Wed May 02 19:09:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro19ala' Tue Jun 30 19:10:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro19Ala' Q924N4-1:p.Lys21Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:30:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys21ala' Tue Jun 30 20:17:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys21Ala' Q924N4-1:p.Ile22Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A Wed May 02 17:53:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile22ala' Tue Jun 30 19:36:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile22Ala' n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association slc12a2-oxsr1-1 2 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association slc12a6-oxsr-1 2 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association slc12a1-oxsr1-1 2 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q13621:p.Arg20Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:15:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg20ala' Tue Jun 30 16:45:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg20Ala' Q13621:p.Phe21Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 20:01:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe21ala' Tue Jun 30 18:22:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe21Ala' Q13621:p.Val23Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 20:10:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val23ala' Tue Jun 30 15:47:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val23Ala' Q13621:p.Ser24Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:47:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser24ala' Tue Jun 30 18:06:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser24Ala' Q13621:p.Val25Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 20:32:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val25ala' Tue Jun 30 16:15:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val25Ala' Q13621:p.Asn27Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A Wed May 02 16:38:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn27ala' Tue Jun 30 18:50:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn27Ala' Q13621:p.Glu28Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:24:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu28ala' Tue Jun 30 17:50:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu28Ala' physical association physical association Sequence of S12a1 given in paper conflicts with that in entry at positions 19 and 24. slc12a6-stk39-3 2 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q924N4-1:p.Arg14Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:55:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg14ala' Tue Jun 30 17:09:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg14Ala' Q924N4-1:p.Phe15Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:17:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe15ala' Tue Jun 30 19:51:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe15Ala' Q924N4-1:p.Val17Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A Wed May 02 18:12:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val17ala' Tue Jun 30 16:10:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val17Ala' Q924N4-1:p.Pro19Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A Wed May 02 18:27:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro19ala' Tue Jun 30 17:21:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro19Ala' Q924N4-1:p.Lys21Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:09:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys21ala' Tue Jun 30 15:42:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys21Ala' Q924N4-1:p.Ile22Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A Wed May 02 18:24:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile22ala' Tue Jun 30 17:54:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile22Ala' physical association physical association slc12a6-stk39-6 4 6 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis range range certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q924N4-1:p.Phe15Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:08:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe15ala' Tue Jun 30 16:51:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe15Ala' ecobb Escherichia coli (strain B / BL21) physical association physical association slc12a2-stk39-2 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association