IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell (0092-8674) 2002 Li J., Wen J., Lease KA., Doke JT., Tax FE., Walker JC. Figure 8(b) walkerj@missouri.edu in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li J., Wen J., Lease KA., Doke JT., Tax FE., Walker JC. Figure 8(b) walkerj@missouri.edu Cell (0092-8674) 2002 BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell (0092-8674) 2002 Li J., Wen J., Lease KA., Doke JT., Tax FE., Walker JC. Figure 8(b) walkerj@missouri.edu arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li J., Wen J., Lease KA., Doke JT., Tax FE., Walker JC. Transgenic arabidopsis plants expressing tagged protein constructs were used in this study. Figure 8(b) walkerj@missouri.edu Cell (0092-8674) 2002 bak1_arath BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 BAK1 SERK3 At4g33430 F17M5.190 Somatic embryogenesis receptor-like kinase 3 ELG Somatic embryogenesis receptor kinase 3 Protein ELONGATED protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITGAIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR DIP protein Q94F62 original sequence version: 119 DIP protein Q94F62 original sequence version: 129 DIP protein Q94F62 original sequence version: 94 DIP protein Q94F62 original sequence version: 100 DIP protein Q94F62 original sequence version: 139 DIP protein Q94F62 original sequence version: 121 DIP protein Q94F62 original sequence version: 141 DIP protein Q94F62 original sequence version: 113 547B3F38FB46E1DD brl1_arath Serine/threonine-protein kinase BRI1-like 1 BRL1 At1g55610 F20N2.4 BRASSINOSTEROID INSENSITIVE 1-like protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKQRWLLVLILCFFTTSLVMGIHGKHLINDDFNETALLLAFKQNSVKSDPNNVLGNWKYESGRGSCSWRGVSCSDDGRIVGLDLRNSGLTGTLNLVNLTALPNLQNLYLQGNYFSSGGDSSGSDCYLQVLDLSSNSISDYSMVDYVFSKCSNLVSVNISNNKLVGKLGFAPSSLQSLTTVDLSYNILSDKIPESFISDFPASLKYLDLTHNNLSGDFSDLSFGICGNLTFFSLSQNNLSGDKFPITLPNCKFLETLNISRNNLAGKIPNGEYWGSFQNLKQLSLAHNRLSGEIPPELSLLCKTLVILDLSGNTFSGELPSQFTACVWLQNLNLGNNYLSGDFLNTVVSKITGITYLYVAYNNISGSVPISLTNCSNLRVLDLSSNGFTGNVPSGFCSLQSSPVLEKILIANNYLSGTVPMELGKCKSLKTIDLSFNELTGPIPKEIWMLPNLSDLVMWANNLTGTIPEGVCVKGGNLETLILNNNLLTGSIPESISRCTNMIWISLSSNRLTGKIPSGIGNLSKLAILQLGNNSLSGNVPRQLGNCKSLIWLDLNSNNLTGDLPGELASQAGLVMPGSVSGKQFAFVRNEGGTDCRGAGGLVEFEGIRAERLERLPMVHSCPATRIYSGMTMYTFSANGSMIYFDISYNAVSGFIPPGYGNMGYLQVLNLGHNRITGTIPDSFGGLKAIGVLDLSHNNLQGYLPGSLGSLSFLSDLDVSNNNLTGPIPFGGQLTTFPVSRYANNSGLCGVPLRPCGSAPRRPITSRIHAKKQTVATAVIAGIAFSFMCFVMLVMALYRVRKVQKKEQKREKYIESLPTSGSCSWKLSSVPEPLSINVATFEKPLRKLTFAHLLEATNGFSAETMVGSGGFGEVYKAQLRDGSVVAIKKLIRITGQGDREFMAEMETIGKIKHRNLVPLLGYCKVGEERLLVYEYMKWGSLETVLHEKSSKKGGIYLNWAARKKIAIGAARGLAFLHHSCIPHIIHRDMKSSNVLLDEDFEARVSDFGMARLVSALDTHLSVSTLAGTPGYVPPEYYQSFRCTAKGDVYSYGVILLELLSGKKPIDPGEFGEDNNLVGWAKQLYREKRGAEILDPELVTDKSGDVELFHYLKIASQCLDDRPFKRPTMIQLMAMFKEMKADTEEDESLDEFSLKETPLVEESRDKEP 8C4DD9231A466AF7 brl1-bak1 1 3 unspecified role unspecified role prey prey gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q94F62:p.Lys317Glu Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain K E Wed May 02 16:55:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys317glu' Tue Jun 30 19:49:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys317Glu' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait mbp-tag maltose binding protein used as a tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9ZWC8:p.Lys911Glu Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain K E Wed May 02 16:41:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys911glu' Tue Jun 30 17:10:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys911Glu' ecoli Escherichia coli (strain K12) physical association physical association Mutations which removed kinase activity of the protein reduced the interaction between the proteins. There appears to not to be a MBP-tagged term in the feature drop down list - revisit when available brl1-bak1-1 2 4 unspecified role unspecified role bait bait flag tag Flag tagged protein flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey gfp-tag green florescent protein tagged protein gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association