IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. EMBO J. (0261-4189) 2001 Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov EMBO J. (0261-4189) 2001 Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. EMBO J. (0261-4189) 2001 Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov EMBO J. (0261-4189) 2001 Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. EMBO J. (0261-4189) 2001 Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov EMBO J. (0261-4189) 2001 Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. EMBO J. (0261-4189) 2001 Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Brosh RM., von Kobbe C., Sommers JA., Karmakar P., Opresko PL., Piotrowski J., Dianova I., Dianov GL., Bohr VA. HeLa nuclear extracts were incubated with the GST-tagged bait fig 2a BroshR@grc.nia.nih.gov EMBO J. (0261-4189) 2001 wrn_human Werner syndrome ATP-dependent helicase WRN RECQ3 RECQL2 Exonuclease WRN RecQ protein-like 2 DNA helicase, RecQ-like type 3 protein protein human Homo sapiens Human MSEKKLETTAQQRKCPEWMNVQNKRCAVEERKACVRKSVFEDDLPFLEFTGSIVYSYDASDCSFLSEDISMSLSDGDVVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATDAYAGFIIYRNLEILDDTVQRFAINKEEEILLSDMNKQLTSISEEVMDLAKHLPHAFSKLENPRRVSILLKDISENLYSLRRMIIGSTNIETELRPSNNLNLLSFEDSTTGGVQQKQIREHEVLIHVEDETWDPTLDHLAKHDGEDVLGNKVERKEDGFEDGVEDNKLKENMERACLMSLDITEHELQILEQQSQEEYLSDIAYKSTEHLSPNDNENDTSYVIESDEDLEMEMLKHLSPNDNENDTSYVIESDEDLEMEMLKSLENLNSGTVEPTHSKCLKMERNLGLPTKEEEEDDENEANEGEEDDDKDFLWPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAHCISEWGHDFRDSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQITCTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQVTGELRKLNLSCGTYHAGMSFSTRKDIHHRFVRDEIQCVIATIAFGMGINKADIRQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNEKFRLYKLKMMAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDNCRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLVEVSRYNKFMKICALTKKGRNWLHKANTESQSLILQANEELCPKKLLLPSSKTVSSGTKEHCYNQVPVELSTEKKSNLEKLYSYKPCDKISSGSNISKKSIMVQSPEKAYSSSQPVISAQEQETQIVLYGKLVEARQKHANKMDVPPAILATNKILVDMAKMRPTTVENVKRIDGVSEGKAAMLAPLLEVIKHFCQTNSVQTDLFSSTKPQEEQKTSLVAKNKICTLSQSMAITYSLFQEKKMPLKSIAESRILPLMTIGMHLSQAVKAGCPLDLERAGLTPEVQKIIADVIRNPPVNSDMSKISLIRMLVPENIDTYLIHMAIEILKHGPDSGLQPSCDVNKRRCFPGSEEICSSSKRSKEEVGINTETSSAERKRRLPVWFAKGSDTSKKLMDKTKRGGLFS DIP protein Q14191 original sequence version: 125 DIP protein Q14191 original sequence version: 175 63F10D19E90AA461 fen1_human Flap endonuclease 1 Flap structure-specific endonuclease 1 Maturation factor 1 DNase IV FEN1 RAD2 protein protein human Homo sapiens Human MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK DIP protein P39748 original sequence version: 106 DIP protein P39748 original sequence version: 112 DIP protein P39748 original sequence version: 160 5154F2F6E57592C5 wrn-fen1-1 2 6 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association wrn-fen1-2 4 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction wrn-fen1-3 1 6 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction wrn-fen1-4 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Both proteins are endogenous