IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. FEBS Lett. (0014-5793) 2001 Taniguchi Y., Yamaguchi A., Hijikata A., Iwasaki H., Kamagata K., Ishiura M., Go M., Kondo T. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species fig 1b, fig 2, table 3, fig 3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Taniguchi Y., Yamaguchi A., Hijikata A., Iwasaki H., Kamagata K., Ishiura M., Go M., Kondo T. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species fig 1b, fig 2, table 3, fig 3 FEBS Lett. (0014-5793) 2001 Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC. FEBS Lett. (0014-5793) 2001 Taniguchi Y., Yamaguchi A., Hijikata A., Iwasaki H., Kamagata K., Ishiura M., Go M., Kondo T. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species fig 1b, fig 2, table 3, fig 3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Taniguchi Y., Yamaguchi A., Hijikata A., Iwasaki H., Kamagata K., Ishiura M., Go M., Kondo T. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species GST- pull down analyzed by SDS-PAGE followed by autoradiography. fig 1b, fig 2, table 3, fig 3 FEBS Lett. (0014-5793) 2001 kaia_syne7 Circadian clock protein KaiA see0009 kaiA Synpcc7942_1218 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MLSQIAICIWVESTAILQDCQRALSADRYQLQVCESGEMLLEYAQTHRDQIDCLILVAANPSFRAVVQQLCFEGVVVPAIVVGDRDSEDPDEPAKEQLYHSAELHLGIHQLEQLPYQVDAALAEFLRLAPVETMADHIMLMGANHDPELSSQQRDLAQRLQERLGYLGVYYKRDPDRFLRNLPAYESQKLHQAMQTSYREIVLSYFSPNSNLNQSIDNFVNMAFFADVPVTKVVEIHMELMDEFAKKLRVEGRSEDILLDYRLTLIDVIAHLCEMYRRSIPRET DIP protein Q79PF6 original sequence version: 52 DIP protein Q79PF6 original sequence version: 58 DIP protein Q79PF6 original sequence version: 81 7C4F3B79FFDF59A2 kaic_syne7 Circadian clock protein kinase KaiC see0011 kaiC Synpcc7942_1216 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MTSAEMTSPNNNSEHQAIAKMRTMIEGFDDISHGGLPIGRSTLVSGTSGTGKTLFSIQFLYNGIIEFDEPGVFVTFEETPQDIIKNARSFGWDLAKLVDEGKLFILDASPDPEGQEVVGGFDLSALIERINYAIQKYRARRVSIDSVTSVFQQYDASSVVRRELFRLVARLKQIGATTVMTTERIEEYGPIARYGVEEFVSDNVVILRNVLEGERRRRTLEILKLRGTSHMKGEYPFTITDHGINIFPLGAMRLTQRSSNVRVSSGVVRLDEMCGGGFFKDSIILATGATGTGKTLLVSRFVENACANKERAILFAYEESRAQLLRNAYSWGMDFEEMERQNLLKIVCAYPESAGLEDHLQIIKSEINDFKPARIAIDSLSALARGVSNNAFRQFVIGVTGYAKQEEITGLFTNTSDQFMGAHSITDSHISTITDTIILLQYVEIRGEMSRAINVFKMRGSWHDKAIREFMISDKGPDIKDSFRNFERIISGSPTRITVDEKSELSRIVRGVQEKGPES DIP protein Q79PF4 original sequence version: 65 DIP protein Q79PF4 original sequence version: 71 DIP protein Q79PF4 original sequence version: 105 855DFE131EFFF3CF kaia-kaic-1 1 4 unspecified role unspecified role bait bait Q79PF4:p.Arg215Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R C Wed May 02 17:02:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg215cys' Tue Jun 30 17:06:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg215Cys' Q79PF4:p.Pro248Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P K Wed May 02 14:43:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro248lys' Tue Jun 30 18:15:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro248Lys' ckabd1 binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey Q79PF6:p.Tyr166Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 18:47:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr166cys' Tue Jun 30 19:56:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr166Cys' physical association physical association kaia-kaic-2 1 3 unspecified role unspecified role prey prey Q79PF6:p.Arg249His mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H Thu Jul 07 12:18:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg249his Wed May 02 15:31:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg249his' Tue Jun 30 18:52:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg249His' 4 unspecified role unspecified role bait bait Q79PF4:p.Thr495Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Thu Jul 07 12:06:48 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was thr495ala Wed May 02 16:00:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr495ala' Tue Jun 30 18:26:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr495Ala' ckabd2 binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Mutations G421R or Y442H on KaiC increases the interaction with KaiA. kaia-kaic-3 2 4 unspecified role unspecified role bait bait ckabd1 binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey 35s radiolabel tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association kaia-kaic-4 2 4 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ckabd2 binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey 35s radiolabel tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association